• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.131-139
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• 2014

This study was performed to determine the effect of dietary supplementation of procyanidin on growth performance, blood characteristics, and immune function in growing pigs. In experiment 1 (Exp. 1), thirty-two crossbred pigs with an initial BW of $19.2{\pm}0.3$ kg were allocated into 4 treatments for an 8-wk experiment: i) CON (basal diet), ii) MOS 0.1 (basal diet+0.1% mannanoligosaccharide), iii) Pro-1 (basal diet+0.01% procyanidin), and iv) Pro-2 (basal diet+0.02% procyanidin). Pigs fed Pro-1 and Pro-2 diets had greater (p<0.05) gain:feed ratio compared with those fed CON or MOS 0.1 diets. Serum creatinine concentration was less (p<0.05) in Pro-2 treatment than those in CON, MOS 0.1 and Pro-1 treatments. In Exp. 2, twelve pigs (BW $13.4{\pm}1.3$ kg) received basal diet with i) 0 (CON), ii) 0.02% (Pro-0.02%), and iii) 0.04% procyanidin (Pro-0.04%) for 4 wk. Concentration of platelets was lower (p<0.05) in the Pro-0.04% group compared to CON at 24 h after lipopolysaccharide (LPS) challenge. In addition, secretion of cytokines from cultured peripheral blood mononuclear cells (PBMC) in the presence or absence of procyanidin was examined. The levels of interleukin (IL)-$1{\beta}$, IL-6 and tumor necrosis factor (TNF)-${\alpha}$ were lower (p<0.05) in Pro (LPS-stimulated PBMCs+procyanidin) than those in CON (LPS-stimulated PBMCs+PBS) at 4 h after LPS challenge. These data suggest that dietary addition of procyanidin improves feed efficiency and anti-inflammatory cytokines of pigs.

• Korean Journal of Veterinary Research
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• v.53 no.4
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• pp.211-216
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• 2013

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.25 no.1
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• pp.83-94
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• 2015

Deduplication, which is a technique of eliminating redundant data by storing only a single copy of each data, provides clients and a cloud server with efficiency for managing stored data. Since the data is saved in untrusted public cloud server, however, both invasion of data privacy and data loss can be occurred. Over recent years, although many studies have been proposed secure deduplication schemes, there still remains both the security problems causing serious damages and inefficiency. In this paper, we propose secure and efficient client-side deduplication with Key-server based on Bellare et. al's scheme and challenge-response method. Furthermore, we point out potential risks of client-side deduplication and show that our scheme is secure against various attacks and provides high efficiency for uploading big size of data.

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.11B
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• pp.689-697
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• 2005

The IETF has created the v6ops Working Group to assist IPv6 transition and propose technical solutions to achieve it. But it's quite problem which security consideration for a stage of IPv4/IPv6 transition and co-existence. There are new security problem threat that it caused by the characteristics of heterogeneity. In this paper, we describe IPv6 transition mechanisms and analyze security problem for IPv6 transition mechanism. also we propose security consideration and new security mechanism. We analyzed DoS and DRDoS in 6to4 environment and presented a address sanity check as a solution. We also showed an attack of address exhaustion in address allocation server. To solve this problem, we proposed challenge-response mechanism in DSTM.

• Toxicological Research
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• v.18 no.2
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• pp.205-213
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• 2002

This study was undertaken to develop a subacute murine model for predicting occurrence of systemic anaphylaxis and to evaluate efficacy of various immunological parameters as the monitoring indices for the occurrence of anaphyalxis. The murine anaphyalxis model was developed through intraperitoneally sensitizing 100 $\mu\textrm{g}$ ovalbumin (OVA) in the presence of 1 mg alum and 300 ng cholera toxin twice a week interval followed by challenging 500 $\mu\textrm{g}$. OVA intravenously. Typical anaphylaxis symptoms were demonstrated at the both BALB/c mice, a strain prone to type-2 response, and C57BL/6 mice. a strain prone to type-1 response. Level of plasma histamine was approximately 50-fold or 30-fold higher in the mice sensitized with OVA than the mice sensitized with alum plus cholera toxin or the saline-treated mice after OVA challenge, respectively. Sensitization and challenge with OVA significantly enhanced plasma leukotriene $B_4$ level but not IgE levels in comparison with the control mice, which indicated the role of leukotriene $B_4$ for progression of anaphyalxis. Furthermore, among mice suffered from anaphylaxis, levels of OVA-specific IgGl were significantly higher in the BALB/c mice than in the C57BL/6 mice, which implied the genetic susceptibility for the induction of systemic anaphylaxis. Conclusively, simultaneous evaluation of histamine, leukotriene $B_4$, and allergen-specific IgG isotype may serve as more efficient monitoring tool for vaccine-related anaphyalxis.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.6
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• pp.83-89
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• 2012

This paper proposes the design of a security-enhanced RFID authentication protocol which meets the privacy protection for tag bearers. The protocol which uses AES(Advanced Encryption Standard) cipher algorithm is based on a three-way challenge response authentication scheme. In addition, three different types of protocol packet formats are also presented by extending the ISO/IEC 18000-3 standard for realizing the security-enhanced authentication mechanism in RFID system environment. Through the comparison of security, it was shown that the proposed scheme has better performance in user data confidentiality, Man-in-the-middle replay attack, and replay attack, and forgery resistance, compared with conventional some protocols. In order to validate the proposed protocol, a digital Codec of RFID tag is also designed based on the protocol. This Codec has been described in Verilog HDL and also synthesized using Xilinx Virtex XCV400E device.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.3
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• pp.627-632
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• 2005

Almost all network systems provide an authentication mechanism based on user ID and password. In such system, it is easy to obtain the user password using a sniffer program with illegal eavesdropping. The one-time password and challenge-response method are useful authentication schemes that protect the user passwords against eavesdropping. In client/ server environments, the one-time password scheme using time is especially useful because it solves the synchronization problem. It is the stability that is based on Square Root problem, and we would like to suggest OPI(One Pass Identification), enhancing the stability for all of the well-known attacks by now including Free-playing attack, off-line Literal attack, Server and so on. OPI does not need to create the special key to read the password. OPI is very excellent in identifying the approved person within a very short time.

• Journal of Internet Computing and Services
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• v.11 no.4
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• pp.23-32
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• 2010

In this paper, we first propose the security enhancing of authentication protocol for Hash based RFID tag, and then a digital Codec for RFID tag is designed based on the proposed authentication protocol. The protocol is based on a three-way challenge response authentication protocol between the tags and a back-end server. In order to realize a secure cryptographic authentication mechanism, we modify three types of the protocol packets which defined in the ISO/IEC 18000-3 standard. Thus active attacks such as the Man-in-the-middle and Replay attacks can be easily protected. In order to verify effectiveness of the proposed protocol, a digital Codec for RFID tag is designed using Verilog HDL, and also synthesized using Synopsys Design Compiler with Hynix $0.25\;{\mu}m$ standard-cell library. Through security analysis and comparison result, we will show that the proposed scheme has better performance in user data confidentiality, tag anonymity, Man-in-the-middle attack prevention, replay attack, forgery resistance and location tracking.

• Journal of Veterinary Science
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• v.22 no.2
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• pp.15.1-15.13
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• 2021

Background: Attenuated Salmonella strain can be used as a vector to transport immunogens to the host antigen-binding sites. Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats. Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination. Results: Among these vaccines inoculated at 5 × 10⁹ CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the non-vaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions. Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.