• Title/Summary/Keyword: Challenge stress

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Major Fe-Superoxide Dismutase (FeSOD) Activity in Pseudomonas putida is Essential for Survival Under Conditions of Oxidative Stress During Microbial Challenge and Nutrient Limitation

  • Kim, Young-Cheol;Kim, Cheol-Soo;Cho, Baik-Ho;Anderson, Anne-J.
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.859-862
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    • 2004
  • An isolate of Pseudomonas putida has been found to aggressively colonize root tips and induce plant resistance to Fusarium wilt. However, P. putida mutants lacking Fe-superoxide dismutase (SOD) or both FeSOD and MnSOD activities are less competitive in root tip colonization. In the current study, the growth of an FeSOD mutant was found to be more sensitive than that of the wild-type or a MnSOD mutant to oxidative stress imposed by paraquat treatment and culturing with the soil fungus Talaromyces flavus, which generates reactive oxygen species. Also, the loss of culturability with an aging stationary-phase culture was greater for a double SOD mutant than an FeSOD mutant, while no reduction in culturability was observed with the wild-type and a MnSOD mutant under the same protracted stationary-phase conditions. Accordingly, it was concluded that FeSOD activity is the major form of SOD in P. putida and plays an essential role in survival under stress conditions when increased oxidative stress is encountered.

An Exploratory Study on Stress Coping of Small Business Owners: Focus on Korean Retail Distributors

  • Mikyoung KIM;Jaeseung MOON;Suyeong KIM
    • Journal of Distribution Science
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    • v.22 no.9
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    • pp.73-84
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    • 2024
  • Purpose: This study aims to comprehensively analyze what small business owners experience in relation to stressors, consequences, coping methods, and situational factors. Research design, data and methodology: In this study, qualitative research methodology was used to conduct in-depth interviews with 15 small business owners who started franchise convenience stores, and classify stressors and consequences, coping methods, and situational factors based on the transactional theory of stress. Results: The results of this study are as follows. First, stressors were classified into challenge stressor and hindrance stressor. Second, stress coping methods were classified into problem-focused and emotion-focused coping methods. Among them, SBOs mainly used problem-focused coping methods. Third, consequences were classified into physiological, emotional, and behavioral tension. Lastly, the moderating factorsin the process of stress coping were resilience, social support, and positive traits. Conclusion: Theoretically, the stressors and coping methods that small business owners experience during the start-up process were confirmed from an integrated theoretical perspective. Practically, building trust relationships with SBOs and providing realistic job introductions are required at the head office level, and on the individual side, efforts are required to strike an appropriate balance between behavioral and emotional stress coping and to form a consensus with family members.

Gene Expression Analyses in Hypothalami of Immobilization-stressed and BoshimgeonbiTang-treated Mice Using cDNA Microarray (구속 스트레스 (immobilization stress)를 가한 rat의 hypothalamus에서의 유전자 발현 및 포심건비탕의 항스트레스 효과에 관한 cDNA microarray 분석)

  • Lee Han Chang;Yeam Mi Jung;Kim Gun Ho;Choi Kang Duk;Lee Seoung Hee;Shim Insop;Lee Hye Jung;Hahm Dae Hyun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.6
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    • pp.1393-1403
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    • 2003
  • The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-Tang on the stress were studied with cDNA microarray analyses on hypothalamus using an immobilization-stress mouse as stress model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hours once a day, and this challenge was repeated for seven consecutive days. The body weights of the immobilization-stress mice were diminished about 25 percent degree as compared to normal ones. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with CyDye/sup TM/ fluorescence dyes (Amersham Bioscience Co., NJ), and then hybridized to cDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix 4000 series scanner and GenePix Pro/sup TM/ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis- and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 3.5 fold. The 20 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Hspe1 (protein folding), Aatk (apoptosis), Dffa (apoptosis), Itgb1 (cell adhesion), Vcam1 (cell adhesion), Fkbp5 (protein folding), BDNF (neuron survival) were restored to the normal one by the treatment of Boshimgeonbi-Tang.

Efficient membrane element for cyclic response of RC panels

  • Tesser, Lepoldo;Talledo, Diego A.
    • Computers and Concrete
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    • v.20 no.3
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    • pp.351-360
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    • 2017
  • This paper presents an efficient membrane finite element for the cyclic inelastic response analysis of RC structures under complex plane stress states including shear. The model strikes a balance between accuracy and numerical efficiency to meet the challenge of shear wall simulations in earthquake engineering practice. The concrete material model at the integration points of the finite element is based on damage plasticity with two damage parameters. All reinforcing bars with the same orientation are represented by an embedded orthotropic steel layer based on uniaxial stress-strain relation, so that the dowel and bond-slip effect of the reinforcing steel are presently neglected in the interest of computational efficiency. The model is validated with significant experimental results of the cyclic response of RC panels with uniform stress states.

Finite Element Analysis of Porthole Extrusion Process for Al Suspension Arm (서스펜션 암의 포트홀 다이 압출공정 유한요소 해석)

  • Joe, Y.J.;Lee, S.K.;Kim, B.M.;Oh, K.H.;Park, S.W.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2006.05a
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    • pp.247-250
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    • 2006
  • The growing demand for more fuel-efficient vehicles to reduce energy consumption and air pollution is a challenge for the automotive industry. The characteristic properties of aluminum, high strengrth stiffness to weight ratio, good formability, good corrosion resistence, and recycling potential make it the ideal candidate to replace heavier materials in the car to respond to the weight resuction demand within the automotive industry. In this paper, A series of compression test was carried out to find the flow stress of A6082 at 300, 400 and $500^{\circ}C$, then we tried to estimate weldability, extrusion load and effective stress of die in the aluminum extrusion process through the 3D FE simulation at non-steady state for aluminum automotive parts.

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항공기 날개 보의 중량경감용 천공 형상연구

  • Lee, Si-Hun;Gong, Du-Hyeon;Sin, Sang-Jun
    • Proceeding of EDISON Challenge
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    • 2017.03a
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    • pp.304-310
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    • 2017
  • In this paper, various webs of I-shaped beam used in aircraft spars are examined. Under the assumption that an aircraft spar is a cantilevered beam with a constant cross-section and is subjected to only bending, four kinds of webs are analyzed for three different sizes. To enable comparison, each hole has the same area and are subjected to the same load by using EDISON 2D Continuum analysis. While circular hole is the most often used, elliptic one is obtained with the minimum von-Mises stress by about 40% decreased. To verify the results gathered by EDISON, comparison was made with ANSYS and analytic predictions obtained with the stress intensity factor K. As comparison shows insignificant discrepancies, it is concluded that a well-designed beam with elliptic holes will be the most efficient spar regarding weight to rigidity ratio in terms of the bending stress.

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풍력 블레이드의 전단 웹 모양의 최적화를 위한 구조 해석에 대한 연구

  • Kim, Jin-Myeong;Song, Seong-Il
    • Proceeding of EDISON Challenge
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    • 2015.03a
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    • pp.217-222
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    • 2015
  • In this paper, a study was conducted for the optimization through shear web of shape the Edison program in wind power blade. We measured the displacement and stress distribution through two optimization methods to select the model with the smallest displacement and stress values. Before running the analysis, We try to find the inflection point through the shear web of the model and then analyze by introducing the geometric nonlinearity. The first optimization variables are introduced by the pitch angle and three web shapes. Third model such a honeycomb structure is good way to get an advantage for bending test. According to a method of previous optimization, third model is chosen and then the thickness of the web and blade as a variable is introduced, it is extracted as a result of displacement and the maximum stress per mass.

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압축하중을 받는 단순 코일 스프링에 관한 해석 결과 및 분석

  • Yun, Jong-Seon;Lee, Nam-Ju
    • Proceeding of EDISON Challenge
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    • 2015.03a
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    • pp.263-265
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    • 2015
  • In this paper, a coil spring of the suspension components of the car is calculated through FEM(Finite Element Method) when a portion of the one is applied by an external load. we analyze the situation by using 'Large Deformation Analysis SW' in the EDISON structural dynamics server. Results of the analysis are about a displacement of the upper spring after deformation and total mass, and we use them to calculate the spring constant and maximum von-Mises stress by using Hooke's law and von-Mises stress equation. Finally, we visualize the relationship between the calculated spring constant and the mass through graphs and this data are beneficial for industries related to the spring.

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Optimal Shape Design of Legs and Stretcher Parts of Chair (의자 다리와 스트레쳐를 중심으로 한 형상 최적화 설계)

  • Lee, Young-Min;Jeong, Hoon
    • Proceeding of EDISON Challenge
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    • 2016.03a
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    • pp.256-261
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    • 2016
  • In this paper, Shape optimal design for a chair with 4 legs and 2 stretchers consisting of stainless steel was conducted. The shape was transformed by identifying stress and deformation for the part of leg and stretcher. In addition, load condition and mesh was designed using Hypermesh. The stress analysis was carried out using CSD_Elast that is one of EDISON program. In seat test, Maximum equivalent stress was showed at the contact part between seat and legs. As a result, a leg cross-section with rectangular and arch was designed. And optimal height of stretcher was found to reduce a deformation. Also, maximum deformation was reduced by designing a stretcher with ellipse cross-section. So, Optimal chair having 4 legs with rectangular cross section and 2 stretchers with ellipse cross section was shown to satisfy the safety ratio.

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Improving productivity in rabbits by using some natural feed additives under hot environmental conditions - A review

  • Magdy Abdelsalam;Moataz Fathi
    • Animal Bioscience
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    • v.36 no.4
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    • pp.540-554
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    • 2023
  • Heat stress is a major challenge to animal production in tropical and subtropical climates. Rabbits suffer from heat stress more than farm animals because they have few sweat glands, and their bodies are covered with thick fur. Intensive farming relies on antibiotics as antimicrobials or growth promoters to increase animals' productivity and health. However, the European Union and many countries have banned or restricted the use of antibiotics in animal feed for human health concerns. Several studies have found that replacing antibiotics in rabbit feed with natural plants or feed additives increases productivity and improves immune capacity, especially under heat stress conditions. Growth performance, immune response, gut microflora, and carcass yield may be increased in rabbits fed a diet supplemented with some natural plants and/or propolis. In this review article, we discuss and summarize the effects of some herbs and plant extracts as alternative feed additives on rabbit productivity, especially for those raised under hot ambient temperatures.