• Knowledge Management Research
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• v.14 no.1
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• pp.39-55
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• 2013

As the importance of intellectual property rights for the 21st century challenge became prominent, many companies have been trying to secure many rights competitively. In particular, application numbers of patent that represents technology has been increased continuously. Korean companies were not exception; mainly in large companies, there have been continued the efforts to grow the number of patent applications in quantitative volume. But the issues that how viable patents the companies have, how effectively the companies manage, and how economically usable the patents are, are totally different from quantitative management level. As such, the issue is connected to how to assess the patent management level of companies. On the other side of quantitative growth of patent that companies hold, there are some problems such as the difficulties to determine if the patents hold substantial values, and the difficulties to determine whether the patent are managed effectively. In addition, as the numbers of patent application and registration of companies are increased, the cost for patent holding and managing increase. It is required to pay continuous attention to the cost of patent management because patent registration fee has a property that increases rapidly with time and burden for patentee become heavier. As a result of analysis and interpretation, we confirmed that quantitative management, particularly the number of patent applicant does not make positive impact on how to use patent after application. Rather, it is observed that the economical usage is influenced positively by the efforts of patent applicants such as considering for the time of patent examination, paying attention to receive patent registration decision. Therefore, this study shows that efforts patent applicants provide in management level after application time are important to maintain the value of patents.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2006.11a
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• pp.305-309
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• 2006

Construction management system has been needed to be anchored and revitalize in these days, since it was entered into a domestic market. We could consider application of CM services as a challenge of construction project system and a way to get to an advanced nation in construction field. Application of CM services could be possible in public projects by a regime. However, in case of private construction projects, it is hard to apply it without understanding of substantial necessity and propriety. so in this study, we find a application method of CM services to private small-midium construction projects, and make CM services item table of CM services for revitalization by examining the cases that applied to in private small-medium construction projects.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.3
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• pp.386-393
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• 2013

This experiment was conducted to evaluate anti-Salmonella enteritidis (anti-SE) bacteriophage as feed additives to prevent Salmonella enteritidis in broilers. The experimental diets were formulated for 2 phases feeding trial, and 3 different levels (0.05, 0.1 and 0.2%) of anti-SE bacteriophage were supplemented in basal diet. The basal diet was regarded as the control treatment. A total of 320 1-d-old male broilers (Ross 308) were allotted by randomized complete block (RCB) design in 8 replicates with 10 chicks per pen. All birds were raised on rice hull bedding in ambient controlled environment and free access to feed and water. There were no significant differences in body weight gain, feed intake and feed conversion ratio (FCR) at terminal period among treatments (p>0.05). Relative weights of liver, spleen, abdominal fat and tissue muscle of breast obtained from each anti-SE bacteriophage treatment were similar to control, with a slightly higher value in anti-SE bacteriophage 0.2%. In addition, a numerical difference of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) and LDL cholesterol level was observed in the 0.2% anti-SE bacteriophage application even though blood profiles were not significantly affected by supplemented levels of anti-SE bacteriophage (p>0.05). In the result of a 14 d record after Salmonella enteritidis challenge of 160 birds from 4 previous treatments, mortality was linearly decreased with increasing anti-SE bacteriophage level (p<0.05), and Salmonella enteritidis concentration in the cecum was decreased with increasing levels of anti-SE bacteriophage (p<0.05). Based on the results of this study, it is considered that supplementation of 0.2% anti-SE bacteriophage may not cause any negative effect on growth, meat production, and it reduces mortality after Salmonella enteritidis challenge. These results imply to a possible use of anti-SE bacteriophage as an alternative feed additive instead of antibiotics in broilers diet.

• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.291-297
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• 2021

Severe outbreaks of porcine epidemic diarrhoea virus (PEDV) have continued to re-emerge worldwide. Because of the high mortality rate of suckling piglets in PEDV outbreaks, the disease causes significant economic losses in the pig industry. The limited pre-existing immunity against this virus is thought to cause an explosive increase in infection in pig farms. This study aimed to evaluate the clinical symptoms of PEDV after intentional exposure (feedback). During the first few days of the outbreak in a breeding pig farm, 14 sows showed watery diarrhoea, and the disease subsequently spread rapidly throughout the barn. Pigs that were intentionally exposed to PEDV (n=251) showed watery diarrhoea (46.6%), reduced appetite (17.5%), and vomiting (6.0%). However, 75 exposed pigs (29.9%) showed no clinical signs of disease. Four weeks after the feedback challenge, 34 sows gave birth to litters of piglets, which survived with no diarrhoea. Five weeks after the start of the outbreak, PEDV was not detected in any of the examined samples, including environmental swabs. Thus, early diagnosis, prompt establishment of herd immunity, and strict application of biosecurity are good practices to reduce the mortality rates among new-born piglets and control economic losses in pig farms showing PEDV outbreaks.

• International Journal of Contents
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• v.8 no.2
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• pp.67-74
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• 2012

Technological substitution is the process by which a radical technology replaces the dominant technology in an industry. The processes of diffusion and substitution have been modeled extensively (Technology & innovation, 2010). However, the formulation of classical quantitative models encompasses only part of the theoretical space. These models impose many simplified constraints to the achievement of analytical resolution. The interior design organization needs to establish a set of technical system requirements by describing the scope of the accessibility needs of the organization against current technology use. Because of complicated design resources and ongoing advances in design technologies, design systems face the challenge of prioritizing new technologies for supporting. The problem is small design organization administration often displays a lack of concern toward the evaluation of technology integration. In this paper, I will identify the influence of a design organization's technology, and predict how future technology will inform, support, and potentially hinder productivity, culture, and work satisfaction within a design organization in the industry. In addition, I will use current design organizational behavior and leadership models to support my predictions. Finally, I will examine a proven approach to assist designers with evaluating technology integration in interior design organization. The goal is to develop a high quality, professional development scorecards for the evaluation. I will conduct both the evaluation of technology integration and CRM performance evaluation is recommended to assess the effectiveness of technology integration. Therefore, the evaluation of integration technologies oriented design hold the promise of solving the organization application integration challenge. The evaluation of integration technology is a significant pattern for processing such a vision. The careful selection of an integration technology for this purpose is crucial in contributing toward the success of such an interior design organization endeavor.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.489-499
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• 2019

Subunit vaccines are safer and more stable than live vaccines although they have the disadvantage of eliciting poor immune response. To develop a subunit vaccine, an effective delivery system targeting the key elements of the protective immune response is a prerequisite. In this study, oxidized carbon nanospheres (OCNs) were used as a subunit vaccine delivery system and tuberculosis (TB) was chosen as a model disease. TB is among the deadliest infectious diseases worldwide and an effective vaccine is urgently needed. The ability of OCNs to deliver recombinant Mycobacterium tuberculosis (Mtb) proteins, Ag85B and HspX, into bone marrow derived macrophages (BMDMs) and dendritic cells (BMDCs) was investigated. For immunization, OCNs were mixed with the two TB antigens as well as the adjuvant monophosphoryl lipid A (MPL). The protective efficacy was analyzed in vaccinated mice by aerosol Mtb challenge with a virulent strain of Mtb and the bacterial burdens were measured. The results showed that OCNs are highly effective in delivering Mtb proteins into the cytosol of BMDMs and BMDCs. Upon immunization, this vaccine formula induced robust Th1 immune response characterized by cytokine profiles from restimulated splenocytes and specific antibody titer. More importantly, enhanced cytotoxic $CD8^+$ T cell activation was observed. However, it did not reduce the bacteria burden in the lung and spleen from the aerosol Mtb challenge. Taken together, OCNs are highly effective in delivering subunit protein vaccine and induce robust Th1 and $CD8^+$ T cell response. This vaccine delivery system is suitable for application in settings where cell-mediated immune response is needed.

• STI Policy Review
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• v.3 no.2
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• pp.130-151
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• 2012

Fraunhofer was founded in 1949 and grew into Europe's largest application-oriented research organization. Fraunhofer currently employs over 20,000 members in Germany, is internationally networked, and manages an R&D budget of over 1,8 Billion Euros per year. An important step for Fraunhofer to become an integral component of the German innovation system was the introduction of the Fraunhofer Model of financing based on a performance-related system of financial management. The underlying model of the allocation and distribution of public funding to Fraunhofer that is subsequently allotted to specific research groups is one of the success factors of Fraunhofer. Fraunhofer is proud of its decentralized organizational model. Fraunhofer is comprised of 60 Institutes in Germany working in different fields, under one legal framework, and with a strong brand value. Every Fraunhofer Institute is affiliated with a German University and every institute director simultaneously holds a chair at the affiliated university. It is a challenge for the headquarter organization to balance the intended competition of individual Fraunhofer Institutes with complementarity cooperation in science among Fraunhofer-Institutes, especially when coming from different knowledge domains; however, this goal results in a significant advantage. The unique strengths of Fraunhofer offer system solutions in a world with increasingly complex R&D challenges. While growing to become the largest organization on Europe to focus on applied research it is the challenge to remain an agile organization that is flexible in organizational structure. Fraunhofer has reached a well-recognized position in the European innovation landscape. It is often referred to by science and governments as a role model for innovation policy and a key element of the latest successes in the German economy that has recovered quicker from the latest economic crisis than most other western economies. The paper explains Fraunhofer as an organizational paradigm and its underlying management model to elaborate on the challenges of managing a research organization. We wish to show how it is possible to transfer the management model and philosophy of Fraunhofer to innovation systems with different framework conditions and challenges. A universal conclusion may be drawn based on the description of Fraunhofer; however, changes in existing structures and innovation systems cannot be implemented over night.

• IMMUNE NETWORK
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• v.5 no.2
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• pp.110-116
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• 2005

Background: As an attempt to develop a strategy to improve the protective immune response to GM-CSF-secreting CT26 (GM-CSF/CT26) tumor vaccine, we have investigated whether the apoptogenic treatment of GM-CSF/CT26 prior to vaccination enhances the induction of anti-tumor immune response in mouse model. Methods: A carcinogeninduced mouse colorectal tumor, CT26 was transfected with GM-CSF gene using a retroviral vector to generate GM-CSF-secreting CT26 (CT26/GM-CSF). The CT26/GM-CSF was treated with ${\gamma}$-irradiation or mitomycin C to induce apoptosis and vaccinated into BALB/c mice. After 7 days, the mice were injected with a lethal dose of challenge live CT26 cells to examine the protective effect of tumor vaccination in vivo. Results: Although both apoptotic and necrotic CT26/GM-CSF vaccines were able to enhance anti-tumor immune response, apoptotic CT26/GM-CSF induced by pretreatment with ${\gamma}$-irradiation (50,000 rads) was the most potent in generating the anti-tumor immunity, and thus 100% of mice vaccinated with the apoptotic cells remained tumor free for more than 60 days after tumor challenge. Conclusion: Apoptogenic pretreatment of GM-CSF-secreting CT26 tumor vaccine by ${\gamma}$-irradiation (50,000 rads) resulted in a significant enhancement in inducing the protective anti-tumor immunity. A rapid induction of apoptosis of CT26/GM-CSF tumor vaccine at the vaccine site might be critical for the enhancement in anti-tumor immune response to tumor vaccine.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.10 no.4
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• pp.21-32
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• 2000

We have seen a lot of developments on computer application areas with the wide spread use of computers and the rapid growth of communication network. It is necessary to use a cryptographic technique for electronic voting, but, at present, despite of its importance electronic voting protocols so far have many shortcomings. In this paper, with the assumption of a trustable voting centers we propose a large-scale and practical electronic voting protocol satisfying protocol requirements, such as secureness, fairness, privacy of voter and correctness. Voters are able to get a vote without revealing their voted information by using the blinding technique. We can find the injustice between a voter and the tallier by using undeniable challenge and responsible protocol. Also, we proposes a secure protocol that compensates a integrity of electronic voting and protects a privacy of voter from outer attacks as using a anonymity of voter.

• Journal of Microbiology and Biotechnology
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• v.27 no.4
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• pp.718-724
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• 2017

The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the C-terminus of scFv57R in order to help it resist adverse stress and improve the stability and half-life. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.