• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.247-256
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• 2021

The recombinant lysozyme-HJL34 proteins were expressed and purified using commercial Escherichia (E.) coli expression system. Stx2e⁺ F18⁺ E. coli, Actinobacillus pleuropneumoniae (APP), Streptococcus (S.) suis, and Clostridium (C.) perfringens strains were isolated from pigs. The minimum inhibitory concentrations (MICs) of the recombinant lysozyme-HJP34 proteins were examined by means of the microtiter plate method, according to the NCCLS recommendations. The possibility of its as the alternatives to antibiotics was tested in piglets. The MICs were determined as 75 ㎍/mL, 300 ㎍/mL, 75 ㎍/mL, 35.5 ㎍/m against Stx2e⁺ F18⁺ E. coli, APP, S. suis, C. perfringens, respectively. A total of 25 piglets were divied 5 groups. The piglets in group A~C were fed with commercial feed and those in groups D, E were fed with commercial feedstuff. All piglets in groups B~E were challenged with virulent Stx2e⁺ F18⁺ E. coli, APP, S. suis strains. Groups C and D were treated with antimicrobial from 24 h after challenge. All piglets in group B died within 3 days after challenge. Among 5 piglets in groups C and D piglets, 80% survived after challenge. Among group E piglets, 60% were alive until the end of this study. Therefore, this study indicates that recombinant lysozyme-HJP34 proteins is a suitable possibility as a feed additive for reduction of diseases by bacterial pathogens in piglet feed.

• Journal of Veterinary Science
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• v.23 no.3
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• pp.49.1-49.13
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• 2022

Background: Porcine circovirus type 2 (PCV2) and Mycoplasma hyopneumoniae (MHP) are economically significant pathogens in the pig industry. The use of combined vaccines against PCV2 and MHP is one of the most effective ways of protecting pigs from both diseases, and it has become a part of general management. Objectives: This study evaluated the efficacy of two new bivalent vaccines of PCV2 and MHP (Myco-X and Myco-XD) in SPF pigs. Myco-X and Myco-XD are a combined vaccine of MHP with PCV2b and PCV2d, respectively. Methods: Sixteen pigs were divided into four groups: Myco-X-vaccinated challenged, Myco-XD-vaccinated challenged, unvaccinated challenged, and unvaccinated unchallenged. Two milliliters of Myco-X were administered intramuscularly, and 0.5 mL of Myco-XD was injected intradermally at 3 wk of age. The pigs were challenged with virulent PCV2d via the intramuscular and intranasal route 4 wk post-vaccination. Results: All vaccinated pigs showed effective reduction of the clinical signs, the PCV2d load in the blood and nasal swab samples, as well as lung and lymphoid tissue lesions in the challenge test. Compared to unvaccinated challenged animals, the vaccinated challenged animals showed significantly higher (p < 0.05) levels of anti-PCV2 IgG, PCV2d-specific interferon-γ (IFN-γ), and anti-MHP IgG. Conclusions: Based on clinical, microbiological, serological, and pathological assessments, this study confirmed that both combined vaccines could protect pigs against PCV2 infection caused by PCV2d. On the other hand, further research on the efficacy evaluation of these new vaccines against the MHP challenge and PCV2d/MHP co-challenge is needed.

• Fisheries and Aquatic Sciences
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• v.25 no.4
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• pp.243-249
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• 2022

Bacillus SW1-1 is a probiotic isolated from shrimp intestines. We investigated the effects of Bacillus SW1-1 coated diets on the growth, feed utilization, innate immunity, hematological parameters and resistance to Edwardsiella tarda in olive flounder (Paralichthys olivaceus). A commercial diet was used as the control (AP0) and two other diets were prepared by coating 0.25% (AP25) or 0.50% (AP50) probiotic powder which contains 1.0 × 10⁷ CFU/g Bacillus SW1-1. Four replicate groups of olive flounder (153 ± 2 g) were fed one of the diets for 12 weeks. Growth performance and feed utilization of the fish were not significantly affected by the dietary Bacillus SW1-1. After the challenge with E. tarda, AP50 group showed significantly higher survival than AP0 and AP25 groups. Innate immunity and anti-oxidant capacity of the fish were not significantly affected after the feeding trial. However, after the E. tarda challenge, the innate immune parameters (immunoglobulin, lysozyme and anti-protease) were significantly improved in fish fed AP25 and AP50 diets compared to those in fish fed AP0 diet. After the challenge test, significantly lower glucose level was observed in AP50 group compared to AP0 group. These results indicate that dietary supplementation of Bacillus SW1-1 could increase the disease resistance of olive flounder against E. tarda infection. The optimum coating levels of Bacillus SW1-1 needs to be further elucidated.

• Journal of the Korean Home Economics Association
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• v.50 no.7
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• pp.1-12
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• 2012

The purpose of this study was to identify the factor structure of self-efficacy, consumer behavior, and the learning effects of the unit 'Consumption Life' and to study the effects of self-efficacy on the consumer behavior, and the learning effects of the unit 'Consumption Life'. Questionnaires were administered to 370 female middle school students living in the Metropolitan City of Daegu. The data was analyzed by using the frequency, descriptive statistics, factor analysis, reliability analysis, multiple regression, and t-test. The findings are as follow. Self-efficacy was composed of five factors such as the capability in work performance, rational performance, fear, anxiety, and the ability to challenge oneself. Consumer behavior was composed of five factors such as emphasis on product display, emphasis on information, emphasis on fashion, emphasis on appearance, and the products/information exchange. The learning effects of the unit 'Consumption Life' was composed of two factors in the economical consumption, and rational consumption. The effects of consumer behavior and the learning effects of the unit 'Consumption Life' on each of the self-efficacy factors like the capability in work performance, rational performance, fear, anxiety, the ability to challenge oneself were explained by factors such as emphasis on product display, emphasis on information, emphasis on fashion, emphasis on appearance and products/information exchange, and economical consumption and rational consumption.

• IEMEK Journal of Embedded Systems and Applications
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• v.14 no.6
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• pp.329-338
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• 2019

Today's embedded software (SW) developments are mostly preceded by composing Software Requirement Specification (SRS). In particular, in the domain of weapon systems, it is essential to have a systematic method for the verification of the SW functionality. To be more specific, it is crucial to check if the SW functionality is implemented as described in SRS, so-called SW suitability verification. Unfortunately, existing static or dynamic SW testing methods are not sufficient to evaluate suitability with SRS since those testings only verify the robustness of the SW codes. In this paper, we propose an automatic embedded SW suitability verification framework which is based on a structured SRS. The major challenge in the automation of this verification framework is how to get rid of ambiguities in SRS. In order to overcome this challenge, we propose a structured SRS description framework and the supporting toolchain for that. We show how the proposed framework is applied to an actual SRS of a weapon system.

• Journal of the Korean Home Economics Association
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• v.43 no.1 s.203
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• pp.53-67
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• 2005

It has been suggested that children's reasons for reading are various; reading efficacy, challenge, curiosity, involvement, importance, recognition, competition, compliance, grades, avoidance and social interactions. To extend previous studies in which only one or two motivational constructs were studied in relation to reading abilities, this study was carried out to examine 11 inter-relationships of motivational constructs and their relationships to reading abilities. Using the MRQ of Wigfield & Guthrie(1997), and the Basic Learning Skills Test of the Korea Educational Development Institute(1989), 334 elementary school children were measured for their reading motivation and abilities. The results showed that 11 motivational constructs were interrelated, which were also related with reading abilities. Out of 11 motivational constructs, importance was able to predict the reading abilities in multiple-regression analyses. From F-tests, those groups with high reading efficacy, challenge, curiosity, involvement, recognition, competition and social interactions outperformed those with low motivational constructs. The findings of this study confirm that children read for various reasons, and internal motivation and social interactions are significantly related with reading abilities. It is proposed that the internal reasons for reading are also more significant factors in explaining reading abilities than external reasons. Finally, cultural influences on reading motivation and comprehension are also discussed.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1998.04a
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• pp.291-300
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• 1998

This paper demonstrates how ambient vibration measurements at a limited number of locations can be effectively utilized to estimate parameters of a finite element model of a large-scale structural system involving a large number of elements. System identification using ambient vibration measurements presents a challenge requiring the use of special identification techniques, which ran deal with very small magnitudes of ambient vibration contaminated by noise without the knowledge of input farces. In the present study, the modal parameters such as natural frequencies, damping ratios, and mode shapes of the structural system were estimated by means of appropriate system identification techniques including the random decrement method. Moreover, estimation of parameters such as the stiffness matrix of the finite element model from the system response measured by a limited number of sensors is another challenge. In this study, the system stiffness matrix was estimated by using the quadratic optimization involving the computed and measured modal strain energy of the system, with the aid of a sensitivity relationship between each element stiffness and the modal parameters established by the second order inverse modal perturbation theory. The finite element models thus identified represent the actual structural system very well, as their calculated dynamic characteristics satisfactorily matched the observed ones from the ambient vibration test performed on a large-scale structural system subjected primarily to ambient wind excitations. The dynamic models identified by this study will be used for design of an active mass damper system to be installed on this structure fer suppressing its wind vibration.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2021.05a
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• pp.614-616
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• 2021

The world faces difficulties in terms of eye care, including treatment, quality of prevention, vision rehabilitation services, and scarcity of trained eye care experts. However, it is difficult to develop a method for classifying various ocular diseases because the existing dataset for retinal image disclosure does not consist of various diseases found in clinical practice. We propose a method for classifying ocular diseases using the Retinal Fundus Multi-disease Image Dataset (RFMiD), a dataset published in the ISBI-2021 challenge. Our goal is to develop a robust and generalizable model for screening retinal images into normal and abnormal categories. The performance of the proposed model shows a value of 0.9782 for the test dataset as an area under the curve (AUC) score.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.3
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• pp.251-260
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• 1998

It is well known that the clinical test for responsibility of accurate fertilization capacity in male partners is very important to diagnose and treat the infertility. However, it has been reported that the traditional semen analysis cannot accurately predict fertilization and pregnancy potential. The present study was performed to evaluate the acrosomal reaction to ionophore challenge (ARIC) test as a prognostic indicator for fertilization of sperm and oocyte in an in vitro fertilization and embryo transfer (IVF-ET) program. From March 1996 to Februry 1997, 30 couples undergoing IVF program were allocated to this study group. All female partners in the study group were 35 years old or less and their serum level of basal follicle stimulating hormone (FSH) and estradiol $(E_2)$ were normal. All the male partners have normal parameters of semen analysis. The ARIC tests were performed on the day of ovum pick up and in vitro insemination in all the male partners. The controlled ovarian hyperstimulation (COH) using luteal long protocol of gonadotropin releasing hormone (GnRH) agonist was used in all couples for IVF-ET. The acrosomal reaction with $10{\mu}l$ of 10% DMSO was induced spontaneously in $10.1{\pm}9.8%$, and acrosomal reaction with calcium ionophore A 23187 was induced in $27.4{\pm}18.1%$, and the ARIC value was $17.4{\pm}16.2%$. There were no significant correlation between the ARIC value and the fertilization rate ($r^2$=0.044, p=0.268). There were also no significant correlation between the ARIC value and the percentage of the grade I, II embryos ($r^2$=0.046, p=0.261). On the basis of above results, it was suggested that ARIC test might not be a useful prognostic indicator for fertilization in IVF-ET in male partners with normal parameters of conventional semen analysis. We guessed that IVF-ET could be performed to the patients primarily without universal appilcation of ARIC test to all male partenrs, and if fertilization failure occurs, the micro assisted fertilization (MAF) such as intracytoplsmic sperm injection (ICSI) might be used as an alternative mode of treatment with acceptable success rate.

• Korean Journal of Veterinary Service
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• v.24 no.1
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• pp.69-82
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• 2001

The result of studying the pathogenicity of Salmonella typhimurium and S enteritidis isolated from domestic animals in Gyeongbuk province were summarized as follows. In Congo-red binding test, S typhimurium had much more rough types than S enteritidis. In colicin production test, 4 strains of S typhimurium were positive but all of S enteritidis were negative. In hemolysin production test, all of S typhimurium and S enteritidis were negative. In Guinea pig serum resistant test, all of S typhimurium and S enteritidis were positive. As a result of pathogenicity test to mice, 54.4% of mice were died. Therefore, S typhimurium and S enteritidis were considered as highly pathogenic. S typhimurium DT104 and S enteritidis PT4 were more pathogenic to mice than other phage types of same serovar. S typhimurium and S enteritidis were considered not so pathogenic for 6-day-old chickens. The recovery rates of Salmonella stains from mice and chickens inoculated were 96.8%, and 54%, respectively. In chickens, proportional to the time from 2 weeks after challenge inoculation, the recovery rates were noticeably decreased.