• Journal of the Korean Operations Research and Management Science Society
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• v.31 no.1
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• pp.91-103
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• 2006

Given a bill of materials (BOM) tree T labeled by the breadth first search (BFS) order from node 0 to node n and a general network ${\Im}=(V,A)$, where V={1,2,...,m} is the set of production facilities and A is the set of arcs representing transportation links between any of two facilities, we assume that each node of T stands for not only a component. but also a production stage which is a possible stocking point and operates under a periodic review base-stock policy, We also assume that the random demand which can be achieved by a suitable service level only occurs at the root node 0 of T and has a normal distribution $N({\mu},{\sigma}^2)$. Then our integrated model of facility location problems and safety stock optimization problem (FLP&SSOP) is to identify both the facility locations at which partitioned subtrees of T are produced and the optimal assignment of safety stocks so that the sum of production cost, inventory holding cost, and transportation cost is minimized while meeting the pre-specified service level for the final product. In this paper, we first formulate (FLP&SSOP) as a nonlinear integer programming model and show that it can be reformulated as a 0-1 linear integer programming model with an exponential number of decision variables. We then show that the linear programming relaxation of the reformulated model has an integrality property which guarantees that it can be optimally solved by a column generation method.

• Journal of Microbiology and Biotechnology
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• v.26 no.8
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• pp.1398-1403
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• 2016

The simultaneous detection and accurate identification of hepatitis A virus (HAV) is critical in food safety and epidemiological studies to prevent the spread of HAV outbreaks. Towards this goal, a one-step duplex reverse-transcription (RT)-PCR method was developed targeting the VP1/P2B and VP3/VP1 regions of the HAV genome for the qualitative detection of HAV. An HAV RT-qPCR standard curve was produced for the quantification of HAV RNA. The detection limit of the duplex RT-PCR method was 2.8 × 10¹ copies of HAV. The PCR products enabled HAV genotyping analysis through DNA sequencing, which can be applied for epidemiological investigations. The ability of this duplex RT-PCR method to detect HAV was evaluated with HAV-spiked samples of fresh lettuce, frozen strawberries, and oysters. The limit of detection of the one-step duplex RT-PCR for each food model was 9.4 × 10² copies/20 g fresh lettuce, 9.7 × 10³ copies/20 g frozen strawberries, and 4.1 × 10³ copies/1.5 g oysters. Use of a one-step duplex RT-PCR method has advantages such as shorter time, decreased cost, and decreased labor owing to the single amplification reaction instead of four amplifications necessary for nested RT-PCR.

• Journal of Radiation Protection and Research
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• v.13 no.2
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• pp.41-51
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• 1988

In order to develop a food-chain computer code suitable to the environmental conditions of Korea, the FOOD III code was partially modified. The excution results for Korean male-adult were compared to those from the Canadian version of FOOD III to deduce a more realistic approach in dose assessment. The amounts of Mn-54, Co-58, Co-60, I-131 and I-132 released from Kori unit 1 in1984 were used as the source terms for the sample calculation. The maximum atmospheric dispersion factor(X/Q) value on the site boundary was applied. Through the code modification, organ doses decreased by about $20{\sim}70%$ and the effective committed dose equivalent by about 40% to be $7.935{\times}10^{-6}Sv/y$ which is 0.16% of the ICRP limit, $5{\times}10^{-3}Sv/y$.

• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.160-170
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• 2019

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

• Journal of Food Hygiene and Safety
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• v.36 no.1
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• pp.93-99
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• 2021

This study was undertaken to compare the efficacy of different sample preparation (stomaching, pulsifying, and sonication) and DNA extraction methods (boiling and commercial kit) for detection of enterotoxin-producing Clostridium perfringens from produce by polymerase chain reaction (PCR). Each produce type was inoculated at concentrations of 102, 103, 104, 105, 106, and 107 spores/g. Produce inoculated with spores was treated with three sample preparation methods, and DNA was extracted by boiling method and a commercial kit, followed by PCR. The detection limit of stomached samples was lower than that of pummeled and sonicated samples by 10-100 times. Moreover, the DNA extraction efficiency of the commercial kit was found to be superior to that of boiling. In particular, the PCR efficiency of cherry tomato and perilla leaf samples was greatly affected by sample preparation and DNA extraction method. These data suggest that DNA extraction with a commercial kit after pulsification is an optimum sample preparation method for detection of C. perfringens by PCR.

• Journal of Construction Engineering and Project Management
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• v.4 no.1
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• pp.37-44
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• 2014

Comparatively, the construction industry has, on average, a higher rate of fatal and major injuries, work induced ill-health and damage to properties than other industrial sectors; and this is a source of concern to industry stakeholders. The study showed that although subcontracting could be contributory to an increase in workplace accidents in the construction industry, contractual aspects of subcontracting arrangements (such as the power imbalance that exist along the client-customer interface) also present opportunities for improvements in health and safety management (HSM) practices in organisations. This conclusion was reached after an analysis of a questionnaire survey (with a 27% response rate) that assessed the attitudes and perception to health and safety issues.

• Journal of information and communication convergence engineering
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• v.15 no.4
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• pp.227-231
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• 2017

Recently, as the number of safety accidents caused by reagents increases, researches on a system that can reduce safety accidents are underway. Existing systems managed reagent cabinet through various sensors. On the other hand, there are disadvantages in that countermeasures against simultaneous danger situations are insufficient at multi-reagents cabinet. In order to solve this problem, this paper proposes a system to manage the reagents cabinet through danger priority. Danger priorities are selected through domestic chemical accident cases and the Chemical Safety Management Act. If a danger situation occurs in the reagent cabinet, make sure it is from a single or multiple reagent cabinets. For multiple reagent cabinets, compare the reagent cabinet priorities and run the device sequentially from the reagent cabinet with the highest priority. Thus, by operating the device according to the danger priority, the chain reaction can be prevented in advance and the reagent cabinet can be safely managed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.4
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• pp.457-466
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• 2015

In this paper, the academic research on the value chain and the supply chain of offshore business are conducted for the investigation of the offshore business comprehensive configuration. Then, the assembly and ergonomic simulation was conducted for the purpose of advanced validation of offshore project.. With respect to the simulation analysis, the assembly simulation model(w.r.t. drill ship) and the ergonomic simulation model(w.r.t. FPSO) are constructed. Through the assembly simulation of drillship, the production processes could be validated in terms of availability. Also, the problem of topside design of FPSO was found by ergonomic simulation.

• Journal of the Korea Safety Management & Science
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• v.5 no.2
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• pp.129-143
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• 2003

Bsusiness-to- Business transactions requires interactions between diverse application s and business. This gives rise to the need to for a common standard and protocol for exchanging busienss messages. and RosettaNet is the leading industry organization focused on developing B2B process standard for a global supply chain. This paper is intended as an investigation of Build-up Method of SCM Connection applying EC Standard(Rossetta-net) to reduce the integration effort, and improve extensibility and interoperability of an eBusiness infrastructure.-Intiative way for E-commerce applications to quickly incorporate new standards. Supply Chain Management (SCM) helps companies plan and execute supply to meet customer demand. The researches of modem scientists have thrown new light on the subject. but, The study of SCM for EC Standard has been strangely neglected by critics. Consequently, existing SCM system, which require to introduce the functions of EC, needs to be changed. Let me summarize the main points that have been made in this paper. The sharing information for connection and the Rosetta-net standard of the existing SCM system, and PIP 3A4, order management module, of the Rosetta-net specifications for connection by the Rosetta-net. Lastly, That shows the process of the gateway for connecting among the existing SCM systems. This provides felxibility in incorporating any new emerging open standard.

• Journal of the Korea Safety Management & Science
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• v.14 no.2
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• pp.91-101
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• 2012

To decrease employees' job stress in the viewpoint of occupational health is very important to improve the productivity and quality of labor. In this study, the job stress, and it's influence on job commitment and organizational commitment were investigated by the questionnaire survey of 259 industrial workers. As results, the majority of workers were under job stress resulted from job demand, job control, and coworker's support. The work times per a week and night work had significant relationships with job demand, and the maximum negative relationship between the work times per a week and job continuous commitment was found. Job demand had the significant relationship with job affective and normative commitment, and job control had the significant relationship with job normative commitment and organizational affective commitment. Moreover, job affective and normative commitment had the significant relationship with organizational continuous commitment. Finally, it was found that cyclic organic chain was composed of work times per a week, night work, salary, job demand, job control, job affective and normative commitment, and organizational affective and continuous commitment. The results of this study indicates that reducing cyclic organic chain is urgently necessary to increase employees' job satisfaction and company commitment.