• Journal of the Korean Society for Precision Engineering
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• v.31 no.1
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• pp.59-66
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• 2014

The central seam, the vertical 'line' between doors, in the front view of a refrigerator must have its gap and flush within certain ranges to meet functional and aesthetic requirements. The conventional criteria for gap and flush control in the industry are to keep the gap and flush within certain ranges at each of various points along the seam. For aesthetics, however, the uniformity of the gap is also as important because a 'tapered' seam is negatively perceived by human eyes. This paper shows a case study of tolerance design for a refrigerator door system. It presents a step-by-step procedure, which consists of datum flow chain analysis, identification of assembly features, computer modeling of feature tolerances, assembly operations and measurements, tolerance simulation, and tolerance adjustments based on the simulation results. It is found that extra care may need to be used to satisfy the aesthetical criterion for gap uniformity.

• Journal of the Optical Society of Korea
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• v.10 no.3
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• pp.105-111
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• 2006

A novel image quality evaluation method, which is based on combination of the rigorous grating diffraction theory and the ray-optic method, is proposed. It is applied for design optimization and, tolerance analysis of optical imaging systems implementing diffractive optical elements (DOE). The evaluation method can predict the quality and resolution of the image on the image sensor plane through the optical imaging system. Especially, we can simulate the effect of diffraction efficiencies of DOE in the camera lenses module, which is very effective for predicting different color sense and MTF performance. Using this method, we can effectively determine the fabrication tolerances of diffractive and refractive optical elements such as the variations' in profile thickness, and the shoulder of the DOE, as well as conventional parameters such as decenter and tilt in optical-surface alignments. A DOE-based 2M-resolution camera lens module designed by the optimization process based on the proposed image quality evaluation method shows ${\sim}15%$ MTF improvement compared with a design without such an optimization.

• Journal of Soil and Groundwater Environment
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• v.21 no.5
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• pp.16-24
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• 2016

A new strain of Pseudomonas sp. was isolated from mercury (Hg)-contaminated sites in Taiwan. This bacterium removed more than 80% of Hg present in the culture medium at 12 h incubation and was chosen for further analysis of the molecular mechanisms of Hg tolerance/removal abilities in this Pseudomonas sp. We used RNA-seq, one of the next-generation sequencing methods, to investigate the transcriptomic responses of the Pseudomonas sp. exposed to 60 mg/L of Hg²⁺. We de novo assembled 4,963 contigs, of which 10,533 up-regulated genes and 5,451 down-regulated genes were found to be regulated by Hg. The 40 genes most altered in expression levels were associated with tolerance to Hg stress and metabolism. Functional analysis showed that some Hg-tolerant genes were related to the mer operon, sulfate uptake and assimilation, the enzymatic antioxidant system, the HSP gene family, chaperones, and metal transporters. The transcriptome were analyzed further with Gene Ontology (GO) and Cluster of Orthologous Groups (COGs) of proteins and showed diverse biological functions and metabolic pathways under Hg stress.

• Journal of Pharmaceutical Investigation
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• v.30 no.1
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• pp.47-50
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• 2000

A new technique has been developed for the preparation of Lactobacillus microcapsules to enhance the stability against high temperature, humidity, gastric acid and bile acid. Employing fluidized bed coating, primary sub-coating was processed in non-organic solvent system, so that Lactobacillus did not directly contact with organic solvent. Secondary enteric-coating was processed in organic solvent with low temperature $(below\;33^{\circ}C)$ technique, which minimized the heat labilability of Lactobacillus. Survival rate of Lactobacillus within microcapsule was not less than 95% and acid tolerance was above 30% in the artificial gastric acid. Further more it was dissolved in the artificial intestine juice within 2-3 hr. Average size of Lactobacillus microcapsules was $450\;{\mu}m$(25-50 mesh) and its viability was above 90% in the direct tableting.

• Journal of the Optical Society of Korea
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• v.18 no.6
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• pp.720-731
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• 2014

There are many kinds of optical systems to widen a field of view. Fisheye lenses with view angles of 180 degrees and omni-directional systems with the view angles of 360 degrees are recognized as proper systems to widen a field of view. In this study, we proposed a new optical system to overcome drawbacks of conventional omni-directional systems such as a limited field of view in the central area and difficulties in manufacturing. Thus we can eliminate the undesirable reflection components of the omni-directional system and solve the primary drawback of the conventional system. Finally, tolerance analysis using Zernike polynomial coefficients was performed to confirm the productivity of the new optical system. Furthermore, we established a method of optical axis alignment and compensation schemes for the proposed optical system as a result of tolerance analysis. In a sensitivity calculation, we investigated performance degradation due to manufacturing error using Code V(R) macro function. Consequently, we suggested compensation schemes using a lens group decentering. This paper gives a good guidance for the optical design and tolerance analysis including the compensation method in the extremely wide angle system.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.302-310
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• 2016

A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a 'rain-out shelter' using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmodulinelike protein (Cam7) and galactinol synthase (Gols4) droughtrelated genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.

• Korean Journal of Computational Design and Engineering
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• v.18 no.5
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• pp.359-366
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• 2013

In the lower arm design process, a tolerance optimization of the variance of design variables should be preceded before manufacturing process, since it is very cost-effective compared to a strict management of tolerance of products. In this study, a design of experiment (DOE) based on response surface model (RSM) was carried out to find optimized design variables of the lower arm, which can meet a given requirement of probability constraint for the process capability index (Cpk) of the weight and maximum stress. Then, the design space was explored by using the central composite design method, in which the 2nd order Taylor expansion was applied to predict a standard deviation of the responses. The optimal solutions satisfying the probability constraint of the Cpk were found by considering both of the mean value and the standard deviation of the design variables.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.67-72
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• 2005

Overwintering capacity, closely related to winter hardiness, of Chinese milk vetch planted with different sowing times and sowing practices was investigated to improve the incorporation into cropping system in Korea. The tolerance to low temperature was evaluated with $LT_50$ using leaf disc leaching method. Dry weight of CMV was reduced remarkably with delayed planting from Sep. 5 to Oct. 20. The differences in tolerance to freezing temperature were not conspicuous among CMV genotypes, however, the differences between genotype (collections at different regions) were due to the plant architecture, mainly to the leaf angle. The crouching genotype collected at central region of Korean peninsula, which showed excellent freezing tolerant, has planophile leaves. The feature of internal constituents of CMV genotypes did not show any noticeable differences with respect to the freezing tolerance which evaluated by leaf disc leaching experiment. To overcome the poor overwintering capacity, tolerant genotype should be developed by selection with considering the plant architecture. The reduction of CMV growth during overwintering period was ameliorated with furrow-sowing under late-sown condition, therefore, when the CMV is inevitably sown late after recommended time, the seeds should be sown on furrow to overcome the cold stress.

• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.53-58
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• 2002

Adverse environmental conditions such as drought, high salt and cold/freezing are major factors that reduces crop productivity worldwide. According to a survey, 50-80% of the maximum potential yield is lost by these "environmental or abiotic stresses", which is approximately ten times higher than the loss by biotic stresses. Thus, improving stress-tolerance of crop plants is an important way to improve agricultural productivity, In order to develop such stress-tolerant crop plants, we set out to identify key stress signaling components that can be used to develop commercially viable crop varieties with enhanced stress tolerance. Our primary focus so far has been on the identification of transcription factors that regulate stress responsive gene expression, especially those involved in ABA-mediated stress response. Be sessile, plants have the unique capability to adapt themselves to the abiotic stresses. This adaptive capability is largely dependent on the plant hormone abscisic acid (ABA), whose level increases under various stress conditions, triggering adaptive response. Central to the response is ABA-regulated gene expression, which ultimately leads to physiological changes at the whole plant level. Thus, once identified, it would be possible to enhance stress tolerance of crop plants by manipulating the expression of the factors that mediate ABA-dependent stress response. Here, we present our work on the isolation and functional characterization of the transcription factors.n factors.

• Proceedings of the Korean Society of Crop Science Conference
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• 2001.05a
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• pp.154-155
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• 2001