• BMB Reports
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• v.40 no.4
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• pp.467-474
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• 2007

Autosomal recessive polycystic kidney disease (ARPKD) is one of the important genetic disorders in pediatric practice. Mutation of the polycystic kidney and hepatic disease gene 1 (PKHD1) was identified as the cause of ARPKD. The gene encodes a 67-exon transcript for a large protein of 4074 amino acids termed fibrocystin, but its function remains unknown. The neoplastic-like in cystic epithelial proliferation and the epidermal growth factor/epidermal growth factor receptor (EGF/EGFR) axis overactivity are known as the most important characteristics of ARPKD. Since the misregulation of $Ca^{2+}$ signaling may lead to aberrant structure and function of the collecting ducts in kidney of rat with ARPKD, present study aimed to investigate the further mechanisms of abnormal proliferation of cystic cells by inhibition of PKHD1 expression. For this, a stable PKHD1-silenced HEK-293T cell line was established. Then cell proliferation rates, intracellular $Ca^{2+}$ concentration and extracellular signal-regulated kinase 1/2 (ERK1/2) activity were assessed after treatment with EGF, a calcium channel blocker and agonist, verapamil and Bay K8644. It was found that PKHD1-silenced HEK-293T cell lines were hyperproliferative to EGF stimulation. Also PKHD1-silencing lowered the intracellular $Ca^{2+}$ and caused EGF-induced ERK1/2 overactivation in the cells. An increase of intracellular $Ca^{2+}$ in PKHD1-silenced cells repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation. Thus, inhibition of PKHD1 can cause EGF-induced excessive proliferation through decreasing intracellular $Ca^{2+}$ resulting in EGF-induced ERK1/2 activation. Our results suggest that the loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD by modulation of intracellular $Ca^{2+}$.

• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.741-746
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• 2008

The objective of this study was to estimate the effects of change in body weight on racing time in Thoroughbred racehorses, using total 8,197 horses and 155,656 racing records collected from Korea Racing Association(KRA). The average body weight of the racehorses was 449kg, and the average body weights of stallion, gelding and mare were 460kg, 454kg and 441kg, respectively. Body weight of stallion was the greatest. The considerable loss of body weight was observed in March, April and May for mare, and in May, Jun and July for stallion and gelding. Overall, the body weight of the racehorses decreased in spring and summer, and increased in autumn and winter. The estimated heritability and repeatability for racing time were 0.237 and 0.525, respectively. The heritability for body weight estimated from total records was 0.612. The estimated heri- tabilities for body weight were 0.472, 0.578 and 0.555 for gelding, stallion and mare, respectively. As the change of body weight was greater than ±10kg, the racing time increased significantly. When the body weight changed more than ±20kg in comparison to the changes of body weight of ±5kg, the racing time increased by 0.3 second. When the change of body weight was ±0.5% of body weight, the racing time was the best(fastest). When the change of body weight was more than ±2.5%, racing performance decreased considerably.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.90-95
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• 1997

A study was conducted to investigate the effects of rice bran dietary fiber on wheat flour rheology and wet noodle quality. More than 99% of rice bran dietary fiber contained smaller particle size than $250\;{\mu}m$. The initial pasting temperature, peak and final viscosities in amylograph, and the water absorption and dough stability in farinograph increased with the increase of rice bran dietary fiber concentration. The lightness values decreased with the increase of rice bran dietary fiber concentration in raw noodles as well as cooked noodles. The addition of rice bran dietary fiber was not effective on the cooked weight and volume of cooked noodles. However, the cooking loss of cooked noodles was the highest in control, and increased with the increase of rice bran dietary fiber concentration. Most of texture parameters (hardness, adhesiveness, gumminess and chewiness) of cooked noodles increased, but the cohesiveness decreased with the increase of rice bran dietary fiber concentration. There were no significant differences in appearance, taste and acceptability of cooked noodles between control and noodles containing 3% and 6% rice bran dietary fiber.

• Clinics in Shoulder and Elbow
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• v.11 no.2
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• pp.150-157
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• 2008

Purpose: We wanted to evaluate the results of surgical treatment for fracture of the scapular body. Materials and methods: The subjects of this study were seven patients with fracture of the scapular body and all these patients were managed surgically. We considered the operative indications as being 100% translation of the lateral border, or $25^{\circ}$ of angular deformity of the glenoid, or 1cm medialization or the variant of the double disruption of the superior scapular suspensory complex. There were five males and two females, and their ages ranged between 40 and 58 years (mean age: 49) with average follow up of 11 months (6~24 months). Two patients sustained multiple fractures of the ribs and spines. The surgical results were evaluated according to the subjective satisfaction, the UCLA score and the Korean shoulder score. Results: The mean UCLA score was 29 (17~33) and the mean Korean shoulder score was 86 (63~94). The self assessment for subjective satisfaction was 7.7 (4~9). There were two complications; one case of screw pull-out without the loss of the fracture fixation and the other case with screw penetration of the glenoid cavity. Conclusion: Surgical treatment may be effective for the management of severely displaced fracture of the scapular body in order to prevent the impairment of the shoulder function caused by the altered glenohumeral and scapulothoracic kinematics.

• Korean Journal of Medicinal Crop Science
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• v.19 no.6
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• pp.456-463
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• 2011

The present study examined the effects of Korean white ginseng (WG, Panax ginseng C. A. Meyer) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered with saline or WG (WG 100 or 300 mg/kg, p.o.) daily for 21 days. The cognitive improving efficacy of WG on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing immunohistochemistries on choline acetyltransferase (ChAT), acetylcholinesterase (AchE), cAMP responsive element binding protein (CREB) and brain derived neurotrophic factor (BDNF). The rats treated with TMT injection (control group) showed impaired learning and memory of the tasks, but the rats treated with TMT injection and WG administration produced significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd and 4th days compared to that of the control group. In the retention test, the WG 100 and WG 300 groups showed significantly increased crossing number around the platform compared to that of the control group (p < 0.001). Consistently with the behavioral data, result of immunohistochemistry analysis showed that WG 100 mg/kg significantly alleviated the loss of BDNF-ir neurons in the hippocampus compared to that of the control group (p < 0.01). Also, treatment with WG has a trend to be increased the cholinergic neurons in the hippocampal CA1 and CA3 areas as compared to that of the control group. These results suggest that WG may be useful for improving the cognitive function via regulation of neurotrophic activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.2
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• pp.211-216
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• 2009

This study was conducted to determine whether Pleurotus ostreatus (oyster mushroom), cultivated in various ratios with herbal extract remnants instead of cotton supplemented with nutrients (the control), improved mycelial growth, mushroom yields and longevity during storage. In addition, we investigated the transfer of medicinal herb components into the mushrooms since they contained non-specific medicinal herbs and their composition could not be controlled. Mushrooms cultivated with 70% and 100% medicinal herb remnants had faster growth rates, higher yields and less failure in the development of the fruit body than the control group. There were no differences in HPLC chromatogram among the methanol extracts of Pleurotus ostreatus in all groups. In addition, glycyrrhizin, an indicative compound of licorice which was a major herb among the herbal remnants, was not detected in any of the extracts. Pleurotus ostreatus that was cultivated with 70% and 100% herbal extract remnants had improved storage longevity in comparison with the control. They exhibited the least weight loss during storage among the groups and they maintained firmness in the stipe and pileus. However, the sources of media did not alter the color difference of the stipe and pileus or the quality index of the outward appearance during storage. In conclusion, cultivating media that contained over 70% of medicinal herb extract remnants increased the growth rates and yields of Pleurotus ostreatus. In addition, these mushrooms had enhanced storage longevity due to their firmness. Therefore, medicinal herb extract remnants should be utilized in the cultivating media of various mushrooms.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.31-41
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• 2016

Damage to structures, such as bridge piers, are increasing rapidly due to the debris moving along rivers at the time of flooding. Therefore, the debris fin, debris deflector and debris sweeper, which are debris reduction systems, were produced in this study and an accumulation experiment was carried out on the experimental channel according to the existence of the reduction system. The debris fin is the reduction system that creates parallel flow on debris accumulated on the bridge to pass through the bridge, which was produced using wood. In addition, the debris deflector was produced using steel pipes and it has the type of detouring the direction of debris. The debris sweeper passes the debris using the magnetic force rotation of a screw-shaped cylindrical structure by water flow and it was produced using acrylic material. The experiment was carried out by analyzing the level of accumulation according to the hardness and dropping method of the debris and comparing the accumulation rate of reduction systems, and the experiment was carried out 5 times. According to the experimental results, there was a difference in the accumulation rate according to the type of reduction system and the shape of debris, and it often depended significantly on the initial shape of debris accumulation. The direct debris reduction effect on the bridge was higher in the order of the debris deflector, debris sweeper and debris fin, but in case of the debris deflector, damage, such as stream turbulence, changes in water level and river bed, and the loss of deflector can occur due to debris accumulated directly on the debris deflector. Therefore, it is necessary to design the debris deflector considering these issues.

• Clinical and Experimental Pediatrics
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• v.46 no.12
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• pp.1274-1278
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• 2003

Syncope in children and adolescents have a common occurrence according for up to 15% before adulthood. Micturition syncope, a kind of situational syncope, can be considered a form of reflex syncope. It can typically occur in healthy young men after rising from bed in the early morning who experience sudden loss of consciousness during or immediately after urination. The mechanism of micturition syncope is not completely understood, but it has been suggested that vasovagal reflex mediated bradycardia and peripheral vasodilation and decreased venous return due to Valsalva effect and standing position lead to the decrease in cerebral blood flow resulting in syncope. The causes of syncope are variable. So complete history taking, physical examination, electrocardiography, exercise stress test, echocardiography, head-up tilt table test, electroencephalography(EEG), brain magnetic resonance image and urodynamic study should be required for the diagnosis of micturition syncope. There were several reports about micturition syncope. However, literature of micturition syncope at the pediatric age has rarely been reported in Korea so far. Therefore, we report a case of a 9-year-old boy with micturition syncope with typical EEG findings of high amplitude delta wave and flattening during syncope.

• Korean Journal of Food Science and Technology
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• v.31 no.4
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• pp.957-963
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• 1999

Effects of cryoprotectants on protein denaturation of soybean curd, tofu, during frozen storage were examined. A whole-coagulated non-press tofu was prepared by adding 2% of isolated soybean protein to soy milk in order to prevent loss of added cryoprotectants. The cryoprotectants added were glocose, glycerol, sorbitol, propylene glycol, and tripolyphosphate. The texture characteristics of soybean curds before and after frozen storage were measured by sensory evaluation and Texture analyzer, and the results were evaluated by response surface methodology (RSM). Glucose, glycerol, sorbitol, and sodium tripolyphosphate were effective as single cryoprotectant, and the mixtures of glucose and sodium tripolyphosphate, and sorbitol and propylene glycol were also effective in minimizing textural change during freezing. Overall, the mixture of cryoprotectants were more effective than single cryoprotectant. According to the RSM, the maximum effect of cryoprotectants in minimizing textural changes during freezing was obtained with the mixture of 2.1% glucose, 6.7% glycerol, 2.1% sorbitol, 0.4% propylene glycol, and 0.3% sodium tripolyphosphate. However, considering the sensory acceptability, the optimum use of cryoprotectants in frozen tofu was 1% glucose, 2% glycerol, 1% sorbitol, 0.2% propylene glycol, and 0.5% sodium tripolyphosphate.

• Journal of the Korea Concrete Institute
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• v.26 no.3
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• pp.369-375
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• 2014

This study examined the changes of microstructural compositions in cement matrix according to the depth from the surface of a reinforced concrete (RC) column exposed to fire. The RC column was exposed to a standard fire for 180 minutes. After the fire test, core samples passing through the column section were obtained. Using the core samples, the remaining fractions of calcium-silicate-hydrates (C-S-H) and calcium hydroxide in cement matrix at the surface, the depth of 40 mm and 80 mm and the center (175 mm) were examined using thermal gravimetric analysis (TGA) and X-ray diffraction analysis (XRDA). Using nuclear magnetic resonance (NMR) technique, the silicate polymerization of C-S-H in cement matrix was also evaluated. The experimental results indicated that the amount of C-S-H loss at the center of column experiencing the transferred fire temperature of $236^{\circ}C$ has been underestimated as the TGA results showed the highest C-S-H contents are located at the depth of 80 mm, where the transferred fire temperature is $419^{\circ}C$. Moreover, the destruction of silicate connections at the center was observed as similar as that at the depth of 40 mm, where the transferred fire temperature was $618^{\circ}C$. This might be attributed to the temperature changes during cooling time after the fire test was neglected. Due to the relatively low thermal conductivity of concrete, the high temperature, which can affect the change of microstructure in cements, will hold longer at the center of the column than other depth.