• 한국잡초학회지
• /
• 제18권1호
• /
• pp.69-75
• /
• 1998

Diphenyl ether계 제초제인 oxyfluorfen에 의해 나타나는 세포내 구성물질의 누출, 엽록소 파괴, 지질과산화작용 등 전형적인 생리적 반응을 재배종 담배를 대상으로 하여 엽령별로 조사하였다. Oxyfluorfen을 담배의 엽절편에 처리하고 암조건에서 12시간 동안 배양한 후 광조건하에서 시간별로 전해물질의 누출을 측정한 결과, 암조건하에서는 전해물질의 누출은 얼어나지 않았으나 광조건하에서 일정 기간의 lag period를 거친 후 배양시간이 경과함에 따라 크게 증가하였다. Oyfluorfen에 의한 전해물질의 누출은 처리 농도의 증가에 따라 증대되었으며 담배 잎에 대한 oxyfluorfen의 효과는 엽령에 따라 크게 다르게 나다나 어린 잎일수록 전해물질의 누출이 뚜렷하였으며 반응의 lag period도 단축되었다. Oxyfluorfen에 의한 담배 잎의 엽록소 함량 감소와 malondialdehyde의 생성도 엽령에 따라 크게 좌우되어 엽령별로 어린 잎일수록 크게 나타났다. 이상의 결과에서와 같이 oxyfluorfen에 의해 담배 잎에 나타나는 생리적 반응이 잎의 엽령에 따라 크게 달라져 담배는 diphenyl ether계 제초제에 대한 내성기작연구에 유용한 식물 재료가 될 것으로 생각된다.

• Preventive Nutrition and Food Science
• /
• 제1권2호
• /
• pp.252-255
• /
• 1996

As an index of freeze-injury of yeast, the leakage of intracellular substances from yeast cells after freeze-thawing was investigated. It was found that much more ultraviolet-absorbing substances leaked out from non-freeze tolerant yeast (NETY) than from freeze-tolerant yeast. Furthermore, the rate of leakage of cellular substances form NFTY during incubation exceeded that of FTY, indicating that NFTY is more susceptible to freeze-injury than FTY during frozen-storage. An apparent degradation of phospholipid was observed during incubation of perfermented frozen-cells of NFTY, while little change of phospholipid occurred in FTY, These results suggested that the difference in the sensitivity of yeast might be due to the strength of cell membrane in terms of the degradation of phospholipid by enzymes, phospholipases, attached to cell membranes.

• ETRI Journal
• /
• 제19권2호
• /
• pp.35-47
• /
• 1997

A power amplifier operating at 3.3 V has been developed for CDMA/AMPS dual-mode cellular phones. It consists of linear GaAs power MESFET's, a new gate bias control circuit, and an output matching circuit which prevents the drain terminal of the second MESF from generating the harmonics. The relationship between the intermodulation distortion and the spectral regrowth of the power amplifier has been investigated with gate bias by using the two-tone test method and the adjacent channel leakage power ratio (ACPR) method of CDMA signals. The dissipation power of the power amplifier with a gate bias control circuit is minimized to below 1000 mW in the range of the low power levels while satisfying the ACPR of less than -26 dBc for CDMA mode. The ACPR of the power amplifier is measured to be -33 dBc at a high output power of 26 dBm.

• The Korean Journal of Physiology and Pharmacology
• /
• 제21권2호
• /
• pp.189-195
• /
• 2017

This study aimed to compare the cellular toxicities of three clinically used dry eye treatments; 3% diquafosol tetrasodium and hyaluronic acid at 0.3 and 0.18%. A methyl thiazolyltetrazoiun (MTT)-based calorimetric assay was used to assess cellular proliferation and a lactate dehydrogenase (LDH) leakage assay to assess cytotoxicity, using Human corneal epithelial cells (HCECs) exposed to 3% diquafosol tetrasodium, 0.3% hyaluronic acid (HA), or 0.18% HA or 1, 6 or 24 h. Cellular morphology was evaluated by inverted phase-contrast light microscopy and electron microscopy, and wound widths were measured 24 h after confluent HCECs were scratched. Diquafosol had a significant, time-dependent, inhibitory effect on HCEC proliferation and cytotoxicity. HCECs treated with diquafosol detached more from the bottoms of dishes and damaged cells showed degenerative changes, such as, reduced numbers of microvilli, vacuole formation, and chromatin of the nuclear remnant condensed along the nuclear periphery. All significantly stimulated reepithelialization of HCECs scratched, which were less observed in diquafosol. Therefore, epithelial toxicity should be considered after long-term usage of diquafosol and in overdose cases, especially in dry eye patients with pre-existing punctated epithelial erosion.

• 한국미생물·생명공학회지
• /
• 제23권4호
• /
• pp.373-377
• /
• 1995

We investigated the survival, $\beta$-galactosidase activity and cellular permeability of lactic acid bacteria such as Lactobacillus acidophilus ATCC 4356, Lactobacillus casei subsp. casei IFO 3533, Streptococcus thermophilus KCTC 2185, Lactobacillus delbrueckii subsp. lactis ATCC 4797, and Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 in anaerobic condition of pH 1.5-3.5 range. Numbers of all tested viable cells did not decrease at pH 3.5, but decreased rapidly at pH 1.5 and pH 2.5 during 2 hour incubation at modified EG medium. Immediately after 2 hour incubation, the decrease in population at pH 1.5 and pH 2.5 was about 6-8 and 5-7 log cycles/ml, respectively. Lactobacillus acidophilus ATCC 4356 showed the higest survival of all tested bacteria. The $\beta$-galactosidase activity from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 and Streptococcus thermophilus KCTC 2185 decreased rapidly at pH 1.5 and 2.5, but there was a little decrease at pH 3.5. The cellular permeability that was measured by the leakage of intracellular materials increased with decrease of pH. These results suggest that the ingested lactic acid bacteria may be destroyed in contact with low pH of gastric acid.

• 한국전자파학회논문지
• /
• 제17권10호
• /
• pp.959-966
• /
• 2006

본 논문은 다이플렉서를 이용하여 디지털 셀룰라 대역($f_o$=880 MHz)과 IMT-2000 대역($f_o$=2,140 MHz) 기지국에서 동시에 사용 가능한 이중 대역 전치 왜곡 선형 전력 증폭기(Predistortion Linear Power Amplifier: PD LPA)의 설계 방법을 제시하였다. 입 출력단에 사용된 다이플렉서는 결함 접지 구조(Defected Ground Structure: DGS)를 이용한 저역 통과 여파기와 높은 Q값을 갖는 캐패시터와 마이크로 스트립 스터브를 이용한 고역 통과 여파기로 이루어져 있다. 입출력 반사 계수 특성을 좋게 하기 위하여 3 dB 하이브리드 결합기를 이용한 반사형 타입의 전치 왜곡 선형화기를 설계하였다. IS-95 CDMA IFA 신호와 WCDMA IFA 신호를 이용하여 각 대역에서 제작된 이중 대역 전치 왜곡 선형 전력 증폭기의 인접 채널 누설비(Adjacent Channel Leakage Ratio: ACLR) 개선 정도를 측정한 결과 880 MHz 대역에서 약 10 dB, 2,140 MHz 대역에서 약 9.36 dB 개선되었다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권4호
• /
• pp.357-363
• /
• 2015

The purpose of this study was to compare the inhibitory effect of bevacizumab on human Tenon's fibroblasts (HTFs) cultured from primary and recurrent pterygium. Cultured HTFs were exposed to 2.0, 5.0, 7.5, and 15.0 mg/mL concentration of bevacizumab for 24 hours. The 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase leakage assays were then performed to assess fibroblast metabolism and viability. The matrix metalloproteinase (MMP), procollagen type I C terminal propeptide (PIP), and laminin immunoassays were performed to examine extracellular matrix production. Changes in cellular morphology were examined by phase-contrast and transmission electron microscopy. Both metabolic activity and viability of primary and recurrent pterygium HTFs were inhibited by bevacizumab in a dose-dependent manner, especially at concentrations greater than 7.5 mg/mL. Both types of HTFs had significant decreases in MMP-1, PIP, and laminin levels. Distinctly, the inhibitory effect of bevacizumab on MMP-1 level related with collagenase in primary pterygium HTFs was significantly higher than that of recurrent pterygium. Significant changes in cellular density and morphology both occurred at bevacizumab concentrations greater than 7.5 mg/mL. Only primary pterygium HTFs had a reduction in cellular density at a bevacizumab concentration of 5.0 mg/mL. Bevacizumab inhibits primary and recurrent pterygium HTFs in a dose-dependent manner, especially at concentrations greater than 7.5 mg/mL. As the primary HTFs produces larger amounts of MMP-1 compared to recurrent HTFs, significant reduction in MMP-1 level in primary pterygium HTFs after exposure to bevacizumab is likely to be related to the faster cellular density changes in primary pterygium HTFs.

• 한국미생물·생명공학회지
• /
• 제31권1호
• /
• pp.90-93
• /
• 2003

The antibacterial activity of chitosan-alginate-Fe(II) complex (CAFC) against Escherichia coli and Staphylococcus aureus, and an opportunistic pathogen, Candida albicans, was investigated. A concentration of 1 mg/1 was needed to inhibit the growth of S. aureus and E. coli, while 100 mg/liter was sufficient for the growth inhibition of Candida albicans. The ion leakage of potassium and phosphate from E. coli cell and the penetration of ethidium bromide dye into it indicate that CAFC might be able to increase the cell permeability and consequently cellular leakage, thus leading to cell plasmolysis. Scanning electronmicroscope showed that E. coli cells treated with CAFC became irregular, swelling and expanded. In a field trial, control piglets showed average mortality of up to 60% within 3 days after the onset of diarrhea. In contrast, CAFC-treated groups without mortality was decreased to average 56% on the 1 st day after the treatment, and average 7% on the 3rd day. After then, piglets with diarrhea was not found.

• Biomolecules & Therapeutics
• /
• 제3권3호
• /
• pp.242-244
• /
• 1995

In vitro cell culture system has been proposed as a promising alternative model to in vivo skin irritation test. These studies were performed to screen the cytotoxicity effects of surfactants using normal human skin fibroblasts. Cell membrane integrity assessed by the leakage of lactate dehydrogenase (LDH) and mitochondrial integrity by MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromides reduction test were affected in a dose dependent manner. The irritation potential of surfactants to human skin patch test, and the changes of capillary permeability by rabbit intradermal safety test were assessed as in vivo methods. Our results suggest that LDH leakage assay and MTT reduction test using cultured human fibroblasts could be predictive for the irritancy of various surfactants in human, and LDH assay is superior correlated with in vivo test (r=0.886) to MTT test with in vivotest (r=0.757).

• Journal of Microbiology and Biotechnology
• /
• 제30권12호
• /
• pp.1827-1834
• /
• 2020

Candida albicans is a major fungal pathogen in humans. In our previous study, we reported that an ethanol extract from Aucklandia lappa weakens C. albicans cell wall by inhibiting synthesis or assembly of both (1,3)-β-D-glucan polymers and chitin. In the current study, we found that the extract is involved in permeabilization of C. albicans cell membranes. While uptake of ethidium bromide (EtBr) was 3.0% in control cells, it increased to 7.4% for 30 min in the presence of the A. lappa ethanol extract at its minimal inhibitory concentration (MIC), 0.78 mg/ml, compared to uptake by heat-killed cells. Besides, leakage of DNA and proteins was observed in A. lappa-treated C. albicans cells. The increased uptake of EtBr and leakage of cellular materials suggest that A. lappa ethanol extract induced functional changes in C. albicans cell membranes. Incorporation of diphenylhexatriene (DPH) into membranes in the A. lappa-treated C. albicans cells at its MIC decreased to 84.8%, after 60 min of incubation, compared with that of the controls, indicate that there was a change in membrane dynamics. Moreover, the anticandidal effect of the A. lappa ethanol extract was enhanced at a growth temperature of 40℃ compared to that at 35℃. The above data suggest that the antifungal activity of the A. lappa ethanol extract against C. albicans is associated with synergistic action of membrane permeabilization due to changes in membrane dynamics and cell wall damage caused by reduced formation of (1,3)-β-D-glucan and chitin.