• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.357-389
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• 1987

In an attempt to clarify the physiological functions of individual isoperoxidases, we have studied enzymatic and immunological properties as well as cellular distribution of isoperoxidases from tobacco callus and Korean radish. The gene expression patterns of isoperoxidases in shoot and non-shoot-forming tobbaco callus were also examined by rabbit reticulocyte lysatein vitro translation system. These results indicate that fraction of translatable poly(A)-isoperoxidase mRNA was increased considerably in shoots. At the present time, at least 6-7 isoperoxidases could be detected from the translation mixture of total cellular RNA, among which only one cell wall localized anodic isoperoxidase (named A3) mRNA was bimorphic mRNA. These data suggest the possible regulation of peroxidase activity during shoot formation by altering the polyadenylation state of mRNA. In case of Korean radish seedlings, poly(A)- peroxidase mRNA were also increased depending upon aging.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7487-7488
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• 2014

• Journal of Korean Society of Industrial and Systems Engineering
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• v.28 no.2
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• pp.94-100
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• 2005

This paper presents the application of integrated mathematical programming approach for the design of cellular manufacturing. The proposed approach is carried out in two phases The first phase concerning exceptional elements(EEs) in cell formation and the second phase facilities layout design. This paper considers the total costs of three important costs for (1) intercellular transfer (2) machine duplication and (3) subcontracting. One of Important issue is the calculation of the number of machines considering the maximum utilization of machines and the available capacity of a machines that can be transferred between cells. Facilities layout design is considered to reflect the real field data taking in to account the operational sequence of the parts to be manufactured. The model is formulated as mixed integer programming that is employed to find the optimal solution.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.4 no.1
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• pp.23-42
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• 1991

This study were done to know the effects of Dangkiyeumja on the in vivo and in vitro immune responses of mice. The recipes of Dangkiyeumja used in this study enhanced such, cellular functions of immunocytes as phagocytic capacity of macrophages, rossett-eforming abilities of splenocytes and metabolic activities of lymphocytes, However, the same recipes decreased the formation of such reactive oxygen intermediates(ROI) as superoxide and hydrogenperoxide from the macrophages. The effects of the same recipes on the in vim immune responses was suppressive on the cellular immune response(CIR)measured by delayed-type hypersensitivity against dinitrofluorobenzene and mildly enhancing for the humoral immune response measured by antibody production against sheep red blood cells. The results of this study could be summarized as follow: 1. Administration of Dangkiyeumja enhanced the phagocytic activity of the murine macrophage. 2. Administration of Dangkiyeumja decreased the formation of ROI in the murine macrophage 3. Administration of Dangkiyeumja increased the number of the splenic rotte forming cells in the mouse. 4. Administration of DangKiyeumja did not effect the antibody production against sheep red blood cells. 5. Administration of Dangkiyeumja depressed the delayed-type hypersenitivity against dinitrofluoro benzene in the mouse. The result of this study suggest that Dangkiyeumja could ameliorate the hypersensitivity reactions by reducing the formation of ROI and decreasing the CIR without affecting the other functions of immunocytes.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.11
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• pp.5253-5270
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• 2016

With device-to-device (D2D) communications, an inactive user terminal can be utilized as a relay node to support multi-hop communication so that connective experience of the cell-edge user as well as the capacity of the whole system can be significantly improved. In this paper, we investigate the spectrum sharing for a cooperative relay assisted D2D communication underlying a cellular network. We formulate a joint relay selection and channel assignment problem to maximize the throughput of the system while guaranteeing the quality of service (QoS) requirements of cellular users (CUs) and D2D users (DUs). By exploiting coalition formation game theory, we propose two algorithms to solve the problem. The first algorithm is designed based on merge and split rules while the second one is developed based on single user's movement. Both of them are proved to be stable and convergent. Simulation results are presented to show the effectiveness of the proposed algorithms.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.229-235
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• 2018

Polo-like kinase 1 (Plk1) has been known to be a critical element in cell division including centrosome maturation, cytokinesis and spindle formation in somatic, cancer, and mammalian embryonic cells. In particular, Plk1 is highly expressed in cancer cells. Plk1 inhibitors, such as BI2536, have been widely used to prevent cell division as an anticancer drug. In this study, the fertilized murine oocytes were treated with BI2536 for 30 min after recovery from the oviduct to investigate the effect of down-regulation of Plk1 in the in vivo-fertilized murine embryos. Then, the localization and expression of Plk1 was observed by immunofluorescence staining. The sperm which had entered into the oocyte cytoplasm did not form male pronuclei in BI2536-treated oocytes. The BI2536-treated oocytes showed significantly lower expression of Plk1 than non-treated control group. In addition, alpha-tubulin and Plk1 gathered around sperm head in non-treated oocytes, while BI2536-treated oocytes did not show this phenomenon. The present study demonstrates that the Plk1 inhibitor, BI2536, hinders fertilization by inhibiting the formation of murine male pronucleus.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.4 no.1
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• pp.84-89
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• 2004

The machine cell formation problem is the problem to group machines into machine families and parts into part families so as to minimize bottleneck machines, exceptional parts, and inter-cell part movements in cellular manufacturing systems and flexible manufacturing systems. This paper proposes a new machine cell formation method based on the adaptive Hamming net which is a kind of neural network model. To show the applicability of the proposed method, it presents some experiment results and compares the method with other cell formation methods. From the experiments, we observed that the proposed method could produce good cells for the machine cell formation problem.

• Journal of Korean Institute of Industrial Engineers
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• v.20 no.2
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• pp.51-64
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• 1994

Cellular manufacturing requires formation of machine cells that can produce families of parts with similar processing requirement. The purpose of cell formation is to create separable machine clusters and part families simultaneously. However, the cell formation process often includes the identification of exceptional elements. This paper presents cell formation method under consideration of alternative routings in FMS which consists of machines capable of multi-processing and parts which require more than one operation. We suggest theorems to calculate the maximum number of machine cell and part family which have no exceptional elements. We also develop a cell formation algorithm which is based on the suggested theorem. A numerical example is provided to illustrate the proposed theorem and algorithm.

• Journal of Plant Biology
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• v.34 no.3
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• pp.201-213
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• 1991

This study has been carried out to investigate the ultrastructural changes, formation of storage materials in endosperm cells with electron microscope during the seed formation of Panax ginseng C.A. Meyer. In the early stage of seed formation with green seed coat, the endosperm was cellular type. Cell plate was largely composed of dictyosome vesicles in early stage of wall formation after mitosis. Central vacuole was gradually subdivided into several small-sized vacuoles. During the differentiation of plastids, some proplastid was replaced by amyloplast with starch grains and lamellar structure. A number of mitochondria with well developed cristae were distributed in cytoplasm. Rough endoplasmc reticulum, dictyosome, microbody, free ribosomes and polysomes were evenly distributed in cytoplasm. Spherical spherosomes were formed from dictyosome containing the lipid materials of even electron density. Protein bodies were formed by interfusing between vacuoles and vesicles derived from rough endoplasmic reticulum which contained the amorphous protein of high electron density.

• Journal of Embryo Transfer
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• v.27 no.3
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• pp.189-192
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• 2012

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, ${\alpha}$-MEM or KnockOut$^{TM}$-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, ${\alpha}$-MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and ${\alpha}$-MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, ${\alpha}$-MEM tended to be more suitable after passage 6. Although ${\alpha}$-MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.