• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.183-188
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• 2002

The ohmic heating of foods for sterilization provides a shorter come-up time compared to conventional thermal processes. The electric fields as well as the heat generated by ohmic heating facilitate germicidal effects. In the present study, the effect of ohmic heating on the structure and permeability of the cell membrane of yeast cells, Saccharomyces cerevisae, isolated from Takju (a traditional Korean rice-beer), was investigated. The ohmic heating was found to translocate intracellular protein materials out of the cell wall, and the amount of exuded protein increased significantly as the electric field increased from 10 to 20 V/cm. As higher frequencies were applied, more materials were exuded. Compared to conventional heating, more amounts of proteins and nucleic acids were exuded when these cells were treated with ohmic heating. The molecular weights of the major exuded proteins ranged from 14 kDa to 18 kDa, as analyzed by Tricine-SDS PAGE. A TEM study also confirmed the leakage of cellular materials, thus indicating irreversible damage to the cell wall by ohmic heating. It was, therefore, concluded that the electric fields generated by ohmic heating induced electroporation, causing irreversible damage to the yeast cell wall and promoting the translocation of intracellular materials.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.278-281
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• 2012

Numerical studies were performed to investigate the three-dimensional front structure and dynamics features of detonation wave propagating in a circular tube such as Pulse Detonation Engine (PDE). By carrying out a series of parametric study using one step irreversible Arrhenius kinetics model, mechanisms of the three-dimensional front structure were investigated for two-, three-, four and six-cell mode detonations. A comparison with two-dimensional results, the effects of slapping transverse waves in radial direction were confirmed. In the all muti-cell modes, the detonation front structures and smoked-records on the wall are formed by the propagation of transverse waves along the wall in clockwise and counter-clockwise while the slapping move in radial direction. And the strength of reflected waves on the curved wall is changed by the multi-dimensional confinement effect.

• Journal of Korean Vacuum Science & Technology
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• v.6 no.2
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• pp.71-75
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• 2002

GaAs polycrystalline thin films with good performance were prepared on conducting glass by hot wall epitaxy (HWE), which were used for solar cell. Electron probe micro-analyzer (EPMA) was applied for the composition, morphology of surface and cross-section of grown films, and X-ray diffraction (XRD) for their phase structure; Raman scattering spectum (RSS) and photoluminescence (PL) were used for evaluating their optical characteristics. The results show that, there is textured structure on the surface of grown GaAs polycrystalline films, which is greatly promised to be suitable for the candidate of solar cell with low cost and high efficiency. It is concluded that the source and substrate at temperature of 900 ~ 930 $\^{C}$ and 500 $\^{C}$ respectively would be beneficial for such films.

• Journal of the Korean Wood Science and Technology
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• v.9 no.1
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• pp.1-12
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• 1981

The structure of spiral thickenings, particulary the appearance, arrangement and orientation of thickenings in compression wood of Torreya nucifera, were studied in detail by light and polarizing microscope, scanning and transmission electron microscope. The results obtained are as follows: (1) Using the inclined sections at an angle of 45 degrees to the fiber axis, it seems that we can not only observe the more accurate transverse view of the thickenings but also investigate the formation of their thickenings. (2) Generally 2-4 pieces of thickenings are projected to the cell lumen as nipple-like appearance in transverse section and are as frequent, well developed, forming pair and have the rope-like appearance in radial surface. (3) The secondary wall of early wood is composed of 3 layers (S1, S2, S3) and orientation of thickening appears S helix but that of late wood is of 2 layers (S1, S2) and that orientation shows Z helix. Above two regions are demaracted at several tracheid cells from the growth ring boundary. (4) Orientation of thickening seems to be a element showing the characteristics of compression wood in Torreya nucifera. (5) It believes that the thickenings of compression wood are integral part of the S3 in early wood tracheids and of the S2 in late wood and have the same orientations as the inner-most microfibrils in these layers. (6) Thickening and cavities seem to be not formed together in a secondary cell wall of same tracheids.

• Proceedings of the Korea Concrete Institute Conference
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• 2003.05a
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• pp.985-990
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• 2003

In order to demonstrate Advanced Spent Fuel Conditioning Process (ACP), shielding facilities such as hot cell suitable to handling radionuclides and process property will be necessary. But the construction of new facilities needs much money, man-power and time, it is now scheduled to remodel the hot cell, which has already been installed and maintained at Irradiated Material Experiment Facility (IMEF) in the Korea Atomic Energy Research Institute (KAERI). The basic structure and concrete shield wall of hot cell partly have been constructed on the base floor in IMEF building in current status. And hot cell after remodeling will be used for carrying out the lab-scale experiment of ACP. The hot cell was built in accordance with 35 curies of fe-59(1.2 MeV) as design criteria of radiation dose limit. But the radioactive source of ACP is expected to be much higher than design criteria of IMEF, shielding ability of the hot cell in the current status is unsatisfactory to the hot test of ACP. Therefore shield wall shall be reinforced with heavy concrete, steel or lead. In this paper, dose rates are calculated according to ACP source, shielding materials, etc., and reinforcement structures are determined considering the current situation of hot cells, installation of shield windows and the easiness of work.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.359-369
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• 2008

The mature fruit wall structures were investigated in Lapsana communis, its six subspecies and the four species of Lapsanastrum (Asteraceae; Lactuceae). Lapsanastrum differs from Lapsana communis in some features: two or three protrudent costae verse equally developed costae, hairly versus glabrous exocarp, respectively. Moreover, Lapsana has no sclerenchymatous‐fiber cells in mesocarp, while Lapsanastrum has sclerenchymatous fiber cells. The differences in fruit wall structure between Lapsana and Lapsanastrum obviously support the separation of Lapsanastrum from Lapsana s. lat.

• The Plant Pathology Journal
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• v.27 no.1
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• pp.8-13
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• 2011

The induced resistance of cucumber leaves treated with oligochitosan to the infection of the cucumber powdery mildew, Sphaerotheca fuliginea, was investigated using transmission electron microscopy. The results showed that when the plants were treated with oligochitosan and challenged with inoculum, a significant decrease of the disease occurred. The mycelial development in the treated leaves was markedly inhibited. The cytoplasm of the powdery mildew mycelium was aggregated, with its organelles disintegrated and the cytoplasm collapsed. The protoplasm in haustoria became electron-dense. Haustoria became malformed, their organelles disintegrated, the hausterial wall thickened and eventually the whole complex necrotized. The host cells produced defence structures and materials associated with infection and a hypersensitive response. The host cell wall was thickened and deeply stained; several layers of papilla structure were produced under the cell wall; dark materials were deposited between the cell wall and plasmalemma; extrahaustorial plasmalemma was deeply stained and extrahaustorial matrix appositions had large deposits of electron-dense material; the cytoplasm was disordered, host organelles disintegrated and eventually the whole host cell disintegrated and necrotized.

• Journal of the Korean Society of Visualization
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• v.17 no.3
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• pp.12-18
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• 2019

Direct numerical simulation of a fully developed turbulent plane Couette-Poiseuille flow with a two-dimensional (2-D) rod-roughened wall is performed to investigate the impacts of the surface roughness. It is shown that the logarithmic region in the mean velocity profile over the rough wall Couette-Poiseuille flow is significantly shortened by the surface roughness compared to that over a turbulent Couette-Poiseuille flow with smooth wall. The Reynolds shear stress over the rough wall Couette-Poiseuille flow is decreased compared to that for a smooth case in the outer layer. These results are attributed to weakened turbulence activity or roll-cell mode over the rough wall Couette-Poiseuille flow near the channel centerline due to suppressed development of u'-structure on the top wall, as documented through spanwise energy spectra of the streamwise velocity fluctuations. Inspection of congregation motion near the bottom wall and time evolution of u'-structure reveal weakened co-supporting cycle for the rough wall case.

• Applied Biological Chemistry
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• v.34 no.1
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• pp.32-37
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• 1991

Cell and intercelullar space enlarged during maturation. The cell of soft persimmon was separated from each other. The degradation of middle lamella exhibited in mature persimmon and small vesicle appeared in cytoplasm of turning and mature persimmon. The middle lamella and cell wall were degraded during softening.

• Journal of Korea Foresty Energy
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• v.23 no.1
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• pp.45-68
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• 2004

The word of lignin is derived from the Latin word 'ligum' meaning wood. Lignin is complex polymer consisting of coniferyl alcohol, sinapyl alcohol and p-coumaryl alcohol unit and has an amorphous, three dimensional network structure which is hard to be hydrolyzed by acid. Lignin is found in the cell wall of plants lignified. The mode of polymerization of these alcohols in the cell wall lead to a heterogeneous branched and cross-linked polymer in which phenyl propane units are linked by carbon-carbon and carbon-oxygen bonds. This polymerization of precursors, p-coumaryl alcohol, coniferyl alcohol and sinapyl alcohol to lignin is formed by enzymic dehydrolyzation. The reaction is initiated by an electron transfer which results in the formation of resonance-stabilized phenoxy radical. The combination of these radicals produces a variety of dimers, trimers and oligomers and so on. Lignin research has been divided into basic and practical application field. The basic studies contains biosynthesis, chemical structure, distribution in the cell wall and reactivity by reductants, oxidants and organic solvents. The application research will be approached the reaction of lignin in various pulp making involving pulp bleaching and its effect on pulp qualities. Lignin also will be studied for the production of fine chemicals, polymer products and the conservation into an energy source like petroleum oil because the amount of lignin produced in pulp making process is more than 51,000,000 tons per year in the world. Both basic and application research must lay emphasis on the development for the utilization of lignin and the pulping process. But these researches can not be completed without understanding lignin structure containing functional groups. Therefore, this paper was focused on the review of lignin formulation which has been studied since 1948 in chronological order. This review was based on monomers, dimers, trimers and tetramers of phenyl propane unit structures which were isolated and identified by different methods from various wood.ious wood.