• Microbiology and Biotechnology Letters
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• v.33 no.1
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• pp.9-15
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• 2005

Chitin deacetylase (CDA; EC 3.5.1.41) catalyzes the hydrolysis of N-acetamide bonds of chitin, converting it to chitosan. Chitosan has several applications in areas such as biomedicine, food ingredients, cosmetics, pharmaceuticals, and agriculture. In this paper, occurrence, assay and purification protocols, enzymatic characteristics, substrate specificity, and mode of action of microbial CDAs have been described. Several lines of evidence have substantiated the biological roles involved in cell wall formation and plant-pathogen interactions for fungal CDAs. The gene structure of CDAs has been compared with other family 4 carbohydrate esterases which deacetylate a wide variety of acetylated poly/oligo-saccharides. The use of CDAs for the conversion of chitin to chitosan, in contrast to the presently used chemical procedure, offers the possibility of a controlled, non-degradable process, resulting in the production of well-defined chitosan oligomers and polymers. Insect pathogen that can secrete high levels of chitin-metab­olizing enzymes including CDA can be a possible alternative for new pest management tools.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.12
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• pp.2039-2046
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• 2003

Storage tank filled with fluid has unique dynamic characteristics compared to general structures, due to the interaction between fluid and structure. The oscillation of the fluid surface caused by external forces is called sloshing, which occurs in moving vehicles with contained liquid masses, such as trucks, railroad cars, aircrafts, and liquid missles. In this study, the evaluation method for the reduction of sloshing, the optimized size and location of wing and diaphragm baffles are suggested based on the experimental results. The experimental device can simulate the translation motion. A rectangular tank and various baffles are fabricated to study on the sloshing characteristics. The forces measured using the load cell at tank wall and those are compared with each other through the Fourier transformation for various conditions. The study of the sloshing of the rectangular tank equipped with baffles is conducted under the same conditions with non-baffled rectangular tank experiment. From the experimental results, the sloshing reduction effect by the baffles is observed. In conclusion in case of diaphragm baffles, the optimized size ratio of the width of baffle to the water height is 0.44 and the installation location has no effect to the damping of sloshing. In case of wing baffles, the optimized size ratio of the width of baffle to the length of a rectangular tank is 0.1 and the optimized location ratio of the baffle to the water height is 0.9.

• Journal of Life Science
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• v.25 no.12
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• pp.1450-1457
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• 2015

To overcome the depletion of fossil fuels and environmental problems in future, the research and production of biofuels have attracted attention largely. Thermophilic microorganisms produce effective and robust enzymes which can hydrolyze plant biomass and survive under harsh bioprocessing conditions. Caldicellulosiruptor bescii, which can degrade unpretreated plants and grow on them, is the one of the best candidates for consolidated bioprocessing (CBP). C. bescii can hydrolyze pectin efficiently as well as the major plant cell wall components, cellulose and hemicelluloses. Many glycosyl hydrolases and carbohydrate lyases with multidomain structure play an important role in plant biomass decomposition. Recently genetic tools for metabolic engineering of C. bescii have developed and bioethanol production from unpretreated biomass is achieved in C. bescii. Here, we review the recent studies for biomass degradation by C. bescii and bioethanol production in C. bescii in order to provide information about metabolic engineering of themophilic bacteria and biofuel development.

• The Journal of the Korean Society for Microbiology
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• v.35 no.4
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• pp.273-282
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• 2000

dTDP-rhamnose provides L-rhamnose to the bridge-like structure between mycolyl arabinogalactan and peptidoglycan of the mycobacterial cell wall. dTDP-rhamnose is composed of glucose-l-phosphate and dTTP by four enzymes encoded by rmlA-D. To determine the region(s) of RmlC protein essential for its dTDP-4-keto-6-deoxyglucose epimerase activity, we overexpressed both whole (202 amino acids) and three different truncated (N-terminal 106 or 150 or C-terminal 97 amino acids) RmlC proteins of Mycobacterium tuberculosis. The RmlC enzyme activity in the soluble lysates of ${\Delta}rmlC$ E. coli strain $S{\Phi}874$ (DE3 PlysS) expressing the wild type or truncated rmlC genes was initially analyzed by three sequential reactions from dTDP-glucose to dTDP-rhamnose in the presence of purified RmlB and RmlD. All three soluble lysates containing the truncated RmlC proteins showed no enzyme activity, while that containing the wild type RmlC was active. This wild type RmlC was then overexpressed and purified. The incubation of the purified RmlC enzyme so obtained with dTDP-4-keto-6-deoxyglucose resulted in the conversion of dTDP-4-keto-rhamnose. The results show that the truncated regions of the RmlC protein are important for the RmlC enzyme activity in M. tuberculosis.

• Journal of Bio-Environment Control
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• v.10 no.2
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• pp.106-110
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• 2001

In an effort to evaluate the economic value and durability of the expanded rice hull as substrates, changes in the physical and chemical properties of material and plant growth in that substrate were studied. Using and electron microscope, the structure of used and new expanded rice hull substrate was examined. Considerable decomposition was found in the substrate which had been used one to three times. Compactness and lowered porosity in the used substrates were probably caused by decomposition. The results of cation analysis showed the possible destruction of cell wall of rice hulls. Abundant $Ca^{2+}$ in the substrates used for two to three times also indicated the possibility of decomposition. In tomato yield comparison, 15.2% more yield of tomato fruit in a new substrates indicated the negative effects of decomposition of one-time used substrates. Yield decreased in the substrates used for three times. if perlite substrates is used for three years before renewal and the cost of the perlite renewal is counted. 65.3% saving in the cost will be realized with the use of an expanded rice hull substrate. Another positive effect of the expanded rice hull substrate is the decrease of environmental contamination.n.

• Journal of Powder Materials
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• v.18 no.5
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• pp.430-436
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• 2011

Joining of NiO-YSZ to 316 stainless steel was carried out with B-Ni2 brazing alloy (3 wt% Fe, 4.5 wt% Si, 3.2 wt% B, 7 wt% Cr, Ni-balance, m.p. 971-$999^{\circ}C$) to seal the NiO-YSZ anode/316 stainless steel interconnect structure in a SOFC. In the present research, interfacial (chemical) reactions during brazing at the NiO-YSZ/316 stainless steel interconnect were enhanced by the two processing methods, a) addition of an electroless nickel plate to NiO-YSZ as a coating or b) deposition of titanium layer onto NiO-YSZ by magnetron plasma sputtering method, with process variables and procedures optimized during the pre-processing. Brazing was performed in a cold-wall vacuum furnace at $1080^{\circ}C$. Post-brazing interfacial morphologies between NiO-YSZ and 316 stainless steel were examined by SEM and EDS methods. The results indicate that B-Ni2 brazing filler alloy was fused fully during brazing and continuous interfacial layer formation depended on the method of pre-coating NiO-YSZ. The inter-diffusion of elements was promoted by titanium-deposition: the diffusion reaction thickness of the interfacial area was reduced to less than 5 ${\mu}m$ compared to 100 ${\mu}m$ for electroless nickel-deposited NiO-YSZ cermet.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.55-58
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• 1985

The anatomical diagnoses derived from the adventitious buds, the dimples, and their surroundings, which break out often in the Populus alba ${\times}$ P. glandulosa, were investigated by the light microscope. In the anatomical structure derived from the adventitious buds, the vessel seldom occured, the diameters of the cell were smaller and the wall thicknesses were thicker than those of the normal woods. The wood fibers around the adventitious buds were crooked, frequently had the septa, and the adjacent rays showed the multiple rows. The wood fibers of the dimples were extremely crooked from pith to the hark direction and the rays showed the multiple rows. The vessels in the line-like discoloration zone around the adventitious buds and the dimples formed the traumatic tyloses.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.15 no.1
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• pp.13-21
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• 2001

The discharge characteristics of 3 electrcdes AC surface discharge plasma display were analyzed. For an unstable state of the discharge which appeared at the maximum discharge voltage, it is found that a parbal erase of the wall charge by the second discharge is a cause. Based on the second discharge, new operation margin considering the interrelation between the address discharge and the display discharge was defined and the validity of it was verified by the experiments. It is necessary to decrease the acklress pulse width for high-speed addressing. However, the operation margin of the ackIress pulse decreases as the pulse width of it becomes narrower. If the address pulse width is wider than l[ps], the operation margin of the display discharge is not related to the address pulse width. From the experimental result, image or 8bit 253 gray level was displayed on PDP with the cell structure of the HDTV class by using the high-speed address ADS drive methcd with pulse width of $1[{\mu}s]$ and the brightness of $560[cd/m^2]$ was obtained. ained.

• Current Photovoltaic Research
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• v.3 no.4
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• pp.121-125
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• 2015

BIPV system is one of the best ways to harness PV module. The BIPV system not only produces electricity, but also acts as a building envelope. Thus, it has the strong point of increasing the economical efficiency by applying the PV modules to the buildings. Bifacial solar cells can convert solar energy to electrical energy from both sides of the module. In addition, it is designed as 3 busbar layout which is the same with ordinary mono-facial soalr cells. Therefore, many of the module manufacturers can easily produce the bifacial solar cells without changing their manufacturing equipment. Moreover, bifacial BIPV system has much potential in building application by utilizing glass to glass structure. However, the performance of bifacial solar cells depends on a variety of factors, ranging from the back surface to surrounding conditions. Therefore, in order to apply bifacial solar cells to buildings, an analysis of bifacial PV module performance should be carried out that includes a consideration of various design elements, and reflects a wide range of installation conditions. As a result it found that the white insulation reflector type can improve the performance of the bifacial BIPV system by 16%, compared to the black insulation reflector type. The performance of the bifacial BIPV was also shown to be influenced by inclination angle, due to changes in both the amount of radiation captured on the front face and the radiation transmitted to the rear face through the transparent space. In this study is limited design condition and installation condition. Accordingly follow-up researches in this part need to be conducted.

• Microbiology and Biotechnology Letters
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• v.40 no.3
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• pp.282-285
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• 2012

In this study, the ability of Chlorella vulgaris and Dunaliella salina to use biomass resources for anaerobic digestive biogas production was examined. The differences in cell wall structure pretreatments affecting the yield of soluble products showed that D. salina is a better candidate for biogas production than C. vulgaris. There was no significant difference between pretreated and non-pretreated D. salina in terms of methane production yield by inocula obtained from anaerobic digestion systems. Therefore, D. salina is a suitable algal biomass for biogas production due to its high biomass productivity, simple pretreatment needs, and easy conversion to biogas.