• Title/Summary/Keyword: Cell wall structure

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Change of Microbial Community on Ozonation of Sewage Sludge to Reduce Excess Sludge Production (폐슬러지 감량화 및 재활용을 위한 오존 처리시 하수슬러지내의 미생물 군집구조의 변화)

  • Hong, Jun-Seok;Lim, Byung-Ran;Ahn, Kyu-Hong;Maeng, Sung-Kyu
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.1
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    • pp.59-65
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    • 2004
  • The change of the microbial community structure in excess sludge of different sewage treatment plants by ozone treatment was investigated by quinone profiles. The resulting ozone dosage ranged from 0.1 to $0.4gO_3/gTS$. In terms of overall sludge reduction, more than 50% reduction of the total sludge mass could be achieved by ozone treatment at $0.4gO_3/gTS$. Quinone concentration and type in sludge of different treatment plants were remarkably decreases with increasing ozone dose. Ubiquinones(UQs)-8, -10 and MK-8 were still remained in the ozonized sludge at $0.4gO_3/gTS$. The results of this study showed that the remaining microorganisms belong to UQs-8, -10 and MK-8 were difficult to destruct cell membrane or wall by ozonation. Fecal Streptococci and Salmonella were not detected at ozone dose of $0.2gO_3/gTS$, but Fecal Coliform was not detected at ozone dose of $0.4gO_3/gTS$.

A STUDY ON THE CHARACTERISTICS OF DIELECTRIC LAYER ON THE DISCHARGE ELECTRODES IN AC PDP (AC PDP 유전층의 절연내력과 투명도에 관한 연구)

  • Lee, Sung-Hyun;Kim, Bang-Ju;Kim, Gyu-Seup;Park, Chung-Hoo;Cho, Jung-Soo
    • Proceedings of the KIEE Conference
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    • 1998.07e
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    • pp.1788-1790
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    • 1998
  • The dielectric layers in AC plasma display panel(AC PDP) are essential to the discharge cell structure, because they protect metal electrodes from sputtering by positive ion bombarding in discharge plasma and form a sheath of wall charges which are essential to memory function of AC PDP. This layer should have high dielectric strength and also be transparent because the luminance of PDP is strongly correlated this layer. In this paper, we discussed the dielectric strength and transparency of the dielectric layer under various conditions. As a result, on the $15{\mu}m$ thickness, the minimum dielectric strength was $29V/{\mu}m$ and the transmittance coefficient was about 80% after $570^{\circ}C$ firing process. It can be proposed that the resonable dielectric thickness in AC PDP is $15{\mu}m$ because it has about 80V margin on the maximum applied voltage.

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Antimicrobial Peptides (AMPs): Peptide Structure and Mode of Action

  • Park, Yoon-Kyung;Hahm, Kyung-Soo
    • BMB Reports
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    • v.38 no.5
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    • pp.507-516
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    • 2005
  • Antimicrobial peptides (AMPs) have been isolated and characterized from tissues and organisms representing virtually every kingdom and phylum. Their amino acid composition, amphipathicity, cationic charge, and size allow them to attach to and insert into membrane bilayers to form pores by 'barrel-stave', 'carpet' or 'toroidal-pore' mechanisms. Although these models are helpful for defining mechanisms of AMP activity, their relevance to resolving how peptides damage and kill microorganisms still needs to be clarified. Moreover, many AMPs employ sophisticated and dynamic mechanisms of action to carry out their likely roles in antimicrobial host defense. Recently, it has been speculated that transmembrane pore formation is not the only mechanism of microbial killing by AMPs. In fact, several observations suggest that translocated AMPs can alter cytoplasmic membrane septum formation, reduce cell-wall, nucleic acid, and protein synthesis, and inhibit enzymatic activity. In this review, we present the structures of several AMPs as well as models of how AMPs induce pore formation. AMPs have received special attention as a possible alternative way to combat antibiotic-resistant bacterial strains. It may be possible to design synthetic AMPs with enhanced activity for microbial cells, especially those with antibiotic resistance, as well as synergistic effects with conventional antibiotic agents that lack cytotoxic or hemolytic activity.

A Study of Recycle of Waste Wood After Cultivating Oak Mushroom (II) - On the Structure of Cellulose Crystal Transformation of the Waste Wood - (표고버섯골목의 재활용에 관한 연구 (II) - 폐골목 세포벽 중의 셀룰로오스 결정의 변태구조 -)

  • Kim, Nam-Hun;Lee, Won-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.23 no.3
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    • pp.1-7
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    • 1995
  • The crystal transformation from cellulose I to cellulose II during alkaline swelling of waste wood, which has been used for cultivating oak mushroom(Cortinellus edodes (Berk.) Ito et Imai), was investigated and compared to that of normal wood by a series of X-ray diffraction analysis. When the sapwood of cultivated wood was treated with 20% NaOH solution for 2 hours, the cellulose I can be easily transformed into Na-cellulose I than normal wood or heartwood of cultivated wood. Certainly the formation of Na-cellulose in wood is proportional to alkali swelling duration, and the formation of cultivated sapwood was faster than that of the other woods. Cellulose I in the sapwood of cultivated wood was easily transformed into cellulose II during mercerization, but the sapwood of normal wood and the heartwood of cultivated wood hardly converted to cellulose II. Namely, most of Na-cellulose I in normal wood can be reconverted to cellulose I in the process of washing and drying. Therefore, it can be concluded from this study that in cell wall lignin and hemicellulose can prevent the alkaline swelling of cellulose in wood and the transformation from cellulose I to cellulose II as well.

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A Study on the Dielectric Breakdown voltage and Transparency of Dielectric Layer in AC PDP (AC PDP 유전층의 절연파괴 전압과 투명도에 관한 연구)

  • Park, Jeong-Hu;Lee, Seong-Hyeon;Kim, Gyu-Seop;Son, Je-Bong;Jo, Jeong-Su
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.1
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    • pp.39-44
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    • 1999
  • The dielectric layers in AC plasma display panel(PDP) are essential to the discharge cell structure, because they protect metal electrodes from sputtering by positive ion bombarding in discharge plasma and form a sheath of wall charges which are essential to memory function of AC PDP. This layer should have high dielectric breakdown voltage, and also be transparent because the luminance of PDP is strongly correlated this layer. In this paper, we discussed the dielectric breakdown voltage and transparency of the dielectric layer under various conditions. As a result, on the $15\mum$ thickness, the minimum dielectric breakdown voltage was 435V and the transmission coefficient was about 80% after $570^{\circ}C$ firing process. It can be proposed that the resonable dielectric thickness in AC PDP is $15\mum$ because it has about 75V margin on the maximum applied voltage.

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Chemical Binding States of Ti and O Elements in Anodic Ti Oxide Films (Ti 양극산화 피막에서 Ti 및 O원소의 화학결합 상태)

  • 유창우;오한준;이종호;장재명;지충수
    • Journal of the Korean institute of surface engineering
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    • v.35 no.6
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    • pp.383-390
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    • 2002
  • To investigate behaviors of Ti and O elements and microstructures of anodic titanium oxide films, the films were prepared by anodizing pure titanium in $H_2$S $O_4$, $H_3$P $O_4$, and $H_2O$$_2$ mixed solution at 180V. The microstructures and chemical states of the elements were analyzed using SEM, X-ray mapping, AFM, XRD, XPS (depth profile). The films formed on a titanium substrate showed porous layers which were composed of pore and wall, And with increasing anodizing time a hexagonal shape of cell structures were dominant and solace roughness increased. From the XRD result the structure of the Ti $O_2$ layer was anatase type of crystal on the whole. In the XPS spectra it was found that Ti and O were chemically binded in forms of Ti $O_2$, TiOH, $Ti_2$ $O_3$ at Ti 2p, and Ti $O_2$, $Ti_2$ $O_3$, $P_2$ $O_{5}$, S $O_4^{2-}$ at O ls respectively. Concentration of Ti $O_2$ decreased as the depth increased from the surface of the oxide film towards the substrate, but to the contrary concentrations of TiOH and $Ti_2$ $O_3$ increased.d.

Purification and Physiochemical Characterization of Melanin Pigment from Klebsiella sp. GSK

  • Sajjan, Shrishailnath;Kulkarni, Guruprasad;Yaligara, Veeranagouda;Lee, Kyoung;Karegoudar, T.B.
    • Journal of Microbiology and Biotechnology
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    • v.20 no.11
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    • pp.1513-1520
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    • 2010
  • A bacterium capable of producing melanin pigment in the presence of L-tyrosine was isolated from a crop field soil sample and identified as Klebsiella sp. GSK based on morphological, biochemical, and 16S rDNA sequencing. The polymerization of this pigment occurs outside the cell wall, which has a granular structure as melanin ghosts. Chemical characterization of the pigment particles showed then to be acid resistant, alkali soluble, and insoluble in most of the organic solvents and water. The pigment got bleached when subjected to the action of oxidants as well as reductants. This pigment was precipitated with $FeCl_3$, ammoniacal silver nitrate, and potassium ferricynide. The pigment showed high absorbance in the UV region and decreased absorbance when shifted towards the visible region. The melanin pigment was further charecterized by FT-IR and EPR spectroscopies. A key enzyme, 4-hydroxyphenylacetic acid hydroxylase, that catalyzes the formation of melanin pigment by hydroxylation of L-tyrosine was detected in this bacterium. Inhibition studies with specific inhibitors, kojic acid and KCN, proved that melanin is synthesized by the DOPA-melanin pathway.

Effect of Processing Method on Change of Water Soluble Dietary Fiber of Fagopyrum tataricum

  • Kim, Dong-Eun;Lee, Beom-Goo;Park, Cheol-Ho;Kang, Wie-Soo
    • Agricultural and Biosystems Engineering
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    • v.6 no.2
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    • pp.70-76
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    • 2005
  • Seed, stem and sprout of F. tataricum were separately milled using the ultra fine mill under the same condition to investigate the effect of roasting or extruding on the particle size, microstructure and water solubility of dietary fiber. The mean particle size of MR (roasting) is increased in stem and sprout, and that of ME (extruding) is increased in seed, compared to that of control. The microscopic views of seed show that control has the spherical shape but ME the larger and irregular shape, and those of stem and sprout show that control has the needle like shape but ME more rounded shape. Water solubility index of ME is much higher than that of control or MR in seed, stem and sprout. It shows that seed, stem and sprout are damaged more in extruding than in roasting, and the starch and cell wall structure must be destroyed to change the water insoluble dietary fiber into the water soluble dietary fiber.

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Thermal instability during the melting process in an isothermally heated horizontal cylinder (등온가열 수평원관내 융해과정동안의 열적 불안정성)

  • Jeong, Jae-Dong;Yu, Ho-Seon;Lee, Jun-Sik
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.6
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    • pp.2046-2056
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    • 1996
  • The constrained melting inside an isothermally heated horizontal cylinder has been repeatedly investigated in many studies only for the moderate Rayleigh numbers. This study extends the range of Rayleigh numbers to systematically investigate the transition during melting processes, especially focusing on the complex multi-cellular flow pattern and thermal instability. The enthalpy-porosity formulation, with appropriate source terms to account for the phase change, is employed. For low Rayleigh numbers, initially developed single-cell base flow keeps the flow stable. For moderate Rayleigh numbers, even small disturbances in balance between thermal buoyance force and viscous force result in branched flow structure. For high Rayleight numbers, Benard type convection is found to develop within a narrow gap between thee wall and the unmelted solid. The marginal Rayleigh number and the corresponding wave number are in excellent agreement with those from linear stability theory.

Structure of Secondary Xylem and Cambial Initials in Pinus koraiensis S. et Z. Grown in Arid Soil (건지에서 자란 잣나무(Pinus koraiensis S. et Z.)의 이기목부 및 형성층구조의 변화)

  • 임동옥
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.35-42
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    • 1993
  • This study deals with the effect of dwarf growth for the wood and the structural changes of cambium in Pinus koraiensis subjected to water stress. The length and wall thickness of tracheary elements in dwarf trees was shorter and thicker than that in normal trees. The redial width and cell number of the annual rings are narrower and smaller in dwarf trees than those in normal trees. In serial tangential sections, the frequency of anticlinal division is low and loss of cambial initials is small in dwarf trees. The length of cambial initials in water deficit, trees are slightly shorter than that in normal trees, and the instructive growth of cambial initials during differentiation of xylem mother cells is inactive in dwarf trees. Thus, it is interpreted that the shortening of tracheary elements in dwarf trees is due to the fact that the length of cambial initials are shortened and the intrusive growth of those during differentiation of xylem mother cells is inactive. The structural variations in the wood of naturally occurring dwarf trees are similar to those of trees subjected to artificial water stress. Therefore, it is suggested that the variation of xylem element in dwarf trees are related to water conditions.

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