• Title/Summary/Keyword: Cell size and density

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Morphometric study of the Leydig cell in Korean native cattle (Bos taurus coreae) (한우 고환 간질세포의 형태계측학적 연구)

  • Tae, Hyun-Jin;Ahn, Dong-Choon;Lee, Young-Deok;Na, Sung-Jin;Park, Byung-Yong;Kim, In-Shik
    • Korean Journal of Veterinary Service
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    • v.39 no.3
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    • pp.141-149
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    • 2016
  • Changes in the Leydig cell from pre-puberty to adulthood were studied in Korean native cattle. Eight groups of male cattle aged 14, 17, 20, 25, 30, 35, 40 and 104 weeks (n=7 cattle per group) after birth were used. The purpose of this study was to obtain quantitative information on the Leydig cell of the Korean native cattle during postnatal development. Testes of cattle were fixed by perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using $1{\mu}m$ section stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average diameter of seminiferous tubules gradually increased with age from 14 ($75.56{\mu}m$) to 104 weeks ($298.9{\mu}m$). The volume density of the seminiferous tubules increased with age from 54.2% at week 14 to 76.9% at week 104. The volume density of the interstitium represents 45.52% at week 14. This proportion progressively diminishes during development to reach a value of 23.14% at week 104. The volume density of Leydig cells decreased almost linearly from 14 (20.71%) to 104 weeks (5.28%). The absolute volume of Leydig cells per testis increased significantly from 14 to 104 weeks. The number of Leydig per testis have almost linearly increased from 14 to 104 weeks. The average volume of a Leydig cell reached maximum size by 104 weeks ($2553{\mu}m^3$). These data suggested development of Leydig cell can be classified as the fetal and immature adult Leydig cells (14~35 weeks), and the adult Leydig cells (40~104 weeks).

Developing Microcellular Foamed Vibration Isolator for DVD by Axiomatic Approach (공리적 접근을 이용한 DVD용 초미세 발포 방진재 개발)

  • Jeong, Pil-Jung;Cha, Seong-Un
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.12
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    • pp.101-106
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    • 2000
  • As the rotational speed of optical disk drive is increasing nowadays, the reliability to vibration and shock becomes more important. For this, various of rubber vibration isolators are being developed by varying the shape and hardness. But it is difficult for the hardness of common used rubber to be lowered below Duro 30 degrees, and because of the shape complexity of rubber vibration isolator there are difficulties of analysis. Microcellular foamed vibration isolator has various cell densities and cell sizes, so it can vary the stiffness and damping coefficient. In addition, its hardness can be lowered below Duro 30 degrees. Axiomatic Approach is very useful design method for designing new product or new process. Axiomatic Approach's character is scientific and analytical method. In this paper, developing process of microcellular foamed vibration isolator for DVD is presented with Axiomatic Approach.

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Investigation of Dark Spots Occurred in Plastic LCDs.

  • Park, Seo-Kyu;Min, Kwan-Sik;Choi, Man-Seob;Im, Jang-Soon;Woo, Sung-Il;Kwon, Soon-Bum
    • 한국정보디스플레이학회:학술대회논문집
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    • 2003.07a
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    • pp.524-527
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    • 2003
  • Plastic LCDs have remarkable advantages as compared to glass LCDs, but there still exist reliability issue. Regarding the reliability issue of plastic LCDs, we investigated the phenomena of dark spots that often occurred in plastic LCDs. It was recognized that dark spots are mainly caused by air bubbles that are induced from outside of LCD panel due to cell volume change with external stress and incomplete encapsulation. We have found that cell gap uniformity, spacer density and size and film deformation are important factors influencing dark spot behavior. In this paper, we discuss the phenomena and origin of dark spots in plastic LCDs

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A Process for the Control of Cell Size of 6061 Al foams by Multi-step Induction Heating Method (다출력 유도가열 공정을 이용한 다공질 6061 알루미늄 합금의 기공 제어 공정)

  • 윤성원;강충길
    • Transactions of Materials Processing
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    • v.12 no.5
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    • pp.449-456
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    • 2003
  • Multi-step induction heating process was applied to the powder compact melting technique as a new heating process to achieve pinpoint accuracy, faster cycle time, repeatability, non-contact and energy-efficient heat in a minimal amount of time. The objective of this study is the establishment of the input data diagram of multi step induction heating process for automation of the fabrication process of 6061 Al foams with desired density. At first, proper induction coil was designed to obtain a uniform temperature distribution over the entire cross sectional area of specimen. By using this coil, foaming experiments were performed to investigate the multi-step induction heating conditions such as capacity, temperature and time conditions of each heating and holding step. On the basis of the obtained multi-step induction heating conditions, relationship between final heating temperature and fraction of porosity was investigated.

Open-Cell Rigid Polyurethane Foam Using Reactive Cell Opening Agents (반응성 기포개방제를 이용한 개방기포형 경질우레탄폼)

  • Ahn, WonSool
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.5
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    • pp.2524-2528
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    • 2013
  • Cell opening characteristics dependent on the cell openers for the conventional formulation of a closed-cell polyurethane foam (PUF) was studied using 1-butanol and lithium salt of 12-hydroxystearic acid (Li-12HSA) as the reactive cell opening agents. While cell openining content of only 10.5 % was obtained for the sample with 4 phr of 1-butanol as the single reactive cell opener, that of 98.0% could be obtained for the sample with 2 phr of Li-12HSA as the reactive co-cell opener. As the results, it showed that a fully open-cell rigid PUF could be obtained by introducing a novel reactive cell opener, having a functional group able to form a bulky side-chain on the urethane networks, without severe loss of mechanical properties of the closed-cell PUF like cell size, bulk density, and thermal conductivity.

The Relationship between Monogenean Skin Parasite Entobdella hippoglossi and Mucous Cell Distribution of its Host the Atlantic Halibut Hippoglossus hippoglossus (Atlantic halibut, Hippoglossus hippoglossus에 기생하는 피부흡충, Entobdella hippoglossi의 기생밀도와 숙주 점액 세포와의 관계)

  • Yoon, Gil-Ha;Sommerville, Christina;Jo, Jae-Yoon
    • Korean Journal of Ichthyology
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    • v.11 no.2
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    • pp.134-138
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    • 1999
  • This study investigated the relationship between attachment site preference of the skin parasite, Entobdella hippoglossi and mucous cell density, moucus cell size and epidermis thickness on the surface of the Atlantic halibut Hippoglossus hippoglossus. Parasites occupying the ventral surface of their host were significantly longer and wider than those found on the other zones of the fish (P<0.05). The mean size of the mucous cells on the front region was significantly greater than the other regions on the dorsal and ventral surface (P<0.05). The average numbers of mucous cells and the epidermal thickness in the skin of the halibut were shown that the front region had significantly higher numbers of mucous cells and thicker layer than the rear region on the dorsal and ventral surface of the halibut, respectively (P<0.05).

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Clinicopathological Significance of Lymphangiogenesis and Tumor Lymphovascular Invasion in Indonesian Breast Cancers

  • Widodo, Irianiwati;Ferronika, Paranita;Harijadi, Ahmad;Triningsih, F.X. Ediati;Utoro, Totok;Soeripto, Soeripto
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.2
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    • pp.997-1001
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    • 2013
  • Background: Lymphangiogenesis, assessed as lymphovascular density (LVD), is the initial step of generalized tumor lymphovascular invasion (LVI). It also involves VEGF-C as the most important protein family. Lymphangiogenesis among breast cancer cases correlations with several clinicopathological factors are important to determine prognosis and treatment strategies, but results have been controversial and require clarification. Aim: To define correlations between VEGF-C expression, LVD and LVI with several clinicopathological parameters from Indonesian breast cancer patients. Materials and Methods: Using a cross-sectional study, a total of 48 paraffin-embedded tissues of breast cancer from Dr. Sardjito General Hospital Indonesia were assessed for VEGF-C expression, LVD and LVI by immunohistochemistry. Correlations of these markers with clinicopathological parameters like patient age, tumor size, lymph node status, grade, ER/PR and Her-2 status, cell proliferation and p-53 expression were investigated by linear analysis. Correlations of VEGF-C expression and LVI with several clinicopathological parameters were analyzed with Coefficient Contingency Chi-Square test. Results: The mean of patients age was 53.0 year, pre and post-menopausal patients accounting for 56.3% and 43.8%, respectively. Some 10.4% were well, 41.7% moderate and 47.9% poorly differentiated. ER positivity was evident in 50% while PR and Her-2 positivity was found in 31.3% and 33.3%, respectively. Breast cancer cells with over-expression of p-53 was 64.6% and with high cell proliferation was 56.3%. Lymph node metastasis was noted in 63.5%, and LVI in 72.9%. Significant correlations were found between LVD and tumor size (p:0.037), grade (p:0.000), lymphnode status (p:0.036), LVI (p:0.003), as well as with p-53 and cell proliferation. There were also significant correlation of VEGF-C (p:0.011) and LVI (p:0.001) with tumor grade. Only ER status was found to have a correlation with tumor size (p:0.027). Conclusions: This study suggested that in Indonesian breast cancer patients, lymphangiogenesis is correlated with tumor size, grade, lymph node status and tumor lymphovascular invasion, the latter also being related with p-53 over expression and high cell proliferation.

Characteristics of Seed Germination and Seedling Growth of Native Hydrangea serrata for. acuminata (자생 산수국의 종자 발아와 유묘 생육 특성)

  • Lee, Seung Youn;Kim, Kwang Jin;Lee, Jeong Sik
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.134-142
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    • 2008
  • This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

Properties of Rigid Polyurethane Foams Synthesized from 4,4 (4,4'-Diphenylmethane Diisocyanate와 Polyether Polyol로부터 제조된 경질 폴리우레탄 폼의 물성)

  • 서원진;정현철;김연희;김우년;최건형
    • Polymer(Korea)
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    • v.26 no.2
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    • pp.185-192
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    • 2002
  • Rigid polyurethane foams (PUFs) were prepared from polymeric 4.4'-diphenyl-methane diisocyanate (PMDI), polyether polyol, 1,4-butane diol, silicone surfactant, and distilled water. The density of the PUF was decreased from 173.7 to 41.7 kg/㎥ with an increase in distilled water from 0.5 to 3.0 php (parts per hundred polyol by weight), respectively, at the 0 php butane diol. The cell size of the PUF increased from 115 to 258 $mu extrm{m}$ with an increase in the amount of distilled water from 0.5 to 3.0 php, respectively, at the 10 php butane diol. It was found that the compressive strength of the PUF increased with the content of distilled water, at the same density. Out of the study for the surfactant effect on the properties of the PUF, it was observed that the cell site of the PUF decreased from 360 to 146 $mu extrm{m}$ with an increase in the amount of the surfactant from 0 to 0.33 php, respectively, but the tell size did not change significantly when the amount of the surfactant exceeded 0.33 php.

A study on selective hybrid-structure film fabricated by 355nm UV-pulsed laser processing (355nm UV 레이저를 이용한 선택적 하이브리드 구조 필름의 제작에 관한 연구)

  • Kim, Myung-Ju;Lee, Sang-Jun;Shin, Bo-Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.5
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    • pp.2979-2984
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    • 2015
  • This paper has presented a new foaming technology of selective hybrid-structured polymer film with expanded pores. The porous structure of closed pore was firstly fabricated by applying the 355nm UV-pulsed laser to 0.1mm thick film that was uniformly mixed with PP pellets, copper powder, and CBA (Chemical Blowing Agent). In order to expand pore size of closed-cell shape, LAMO(Laser Aided Micro pore Opening) processing was conducted to heat the copper powder, and then the bigger pore size of closed-cell more than existing pore size was successfully formed because of rapid conduction of heated metal powder. From the experimental results, various process parameters such as laser fluence, intensity, scan rate, spot size and density of powder and CBA were considerably considered to reveal the correlation among the pore characteristics. In the future, a function experiment will be carried out to use the hybrid film of industrial applications.