• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.2
• /
• pp.84-91
• /
• 2000

Mycopasma contamination of tissue culture cells easily evades detection and, thus, represents a continous therat to cell biologists. In case where infected cell can not simply be replaced, attempts have to be made to eradicate mycoplacma from the tissue culture cells. A variety of anti-microbial agents have been shown to be toxic to mycoplasma strains ; however, cell associated mycoplasma are often protected from antibiotics at concentrations shown to be effective in vitro. Antibiotic concentrations high enough to be lethal to cell as sociated mycoplasmas frequently are also detrimentrations to the host cells, while moderately increased antibiotic levels tolerated by the host cells often lead to only temporary growth suppression and/or to the emergence of mycoplasma strains resistanct even to high concentrations of the antibiotis applied. Hare, a genetic approach for the elimination of mycoplasma from tissue culture cells that overcomes thens limitations is described. By expression of a selection marker conferring resistance to an otherwise toxic agent, Acholeplasma laidlawii infected BHK-21 cells used as the model system were enabled to temporarily tolerate antibiotic concentrations high enough to be lethal to cell associated mycopalsma while leaving the host cells unharmed. Upon successful mycoplasma eradicated, cultvation of the cured host cells in the absence of the selective agent yielded revertant cell clones that had regained susceptibillity to the toxic agent. Cressation of the selection marker expression was shown to result from the loss of the selection marker DNA, which is a consequence of the fact that the stable and permanent integration of foreign DNA in eucaryotic cell chrosomes is highly inefficient. Thus, the cells were cured from mycoplasma yet remained biochemically unaltered.

• Journal of Plant Biotechnology
• /
• v.5 no.1
• /
• pp.43-49
• /
• 2003

Resistant cell lines to azetidine-2-carboxylic acid (AZCA) were selected through rice embryo culture after mutagenic treatment of callus irradiated with 30,50,70,90 and 120 Gy. The optimum AZCA concentration for the selection of resistant cell lines was 3 or 4 mM AZCA considering $LD_{50}$ and the fresh weight of callus. Survival rate of the AZCA resistant callus showed remarkable increase in the callus irradiated with 50 and 70 Gy. Regeneration rate of the AZCA resistant callus was much lower on the whole. Ninety and 120 Gy increased the regeneration rate for calli selected from 3 and 4 mM AZCA, respectively. Based on fresh weight, survival rate and regeneration for selection of the AZCA resistant cell line, 50-90 Gy was considered as the optimum range of gamma irradiation. Irradiated calli selected from AZCA were more tolerant to NaCl than those from non-irradiated calli. It suggests that elevated resistance to osmotic stress resulted from mutagenic treatment. The level of free proline content in the AZCA resistant cell line was increased up to 3.5 times compared with that in the control. Proline content in the regenerant derived from the AZCA resistant cell line also increased to 1.7 times that from the control plants regenerated from callus grown in AZCA free medium. Selection of proline overproducing cell lines by in vitro mutagenesis was successful and seems to be useful for improvement of stress tolerance in this crop.

• Journal of Plant Biotechnology
• /
• v.4 no.3
• /
• pp.123-127
• /
• 2002

Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.18 no.10
• /
• pp.2397-2402
• /
• 2014

Recently, heterogeneous mobile communication network is attracting plenty of interest to serve explosively increasing mobile data traffic. Although the heterogeneous mobile communication network can enhance spatial reuse ratio by using both conventional macro cells and small cells simultaneously, it causes the unbalance in performance of downlink and uplink. In this paper, we propose a new cell selection scheme for heterogeneous mobile communication network. In the proposed cell selection scheme, mobiles select their home cells by considering both uplink and downlink performance, contrary to conventional schemes. We analyze the performance of the proposed scheme in terms of average cell transmission rate through system level simulations and compare it with those of conventional schemes.

• Journal of Internet Computing and Services
• /
• v.23 no.5
• /
• pp.9-15
• /
• 2022

This paper proposes a coordinated multi-point communication (CoMP) method with channel selection to improve performance of a macro user equipment (MUE) in a dense small-cell network environment in a building located within coverage of a macro base station (MBS). In the proposed CoMP method, in order to improve the performance of the MUE located in the building, A small-cell base station (SBS) selects a channel with lower interference to the neighboring MUE and transmits appropriate signals to the MUE requiring CoMP. Simulation results show that the proposed CoMP method improves the performance of the MUE by up to 164% and 51%, respectivley, compared to a random channel allocation based traditional SBS network and CoMP method.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.37B no.10
• /
• pp.956-964
• /
• 2012

An opportunistic interference alignment based on dynamic cell selection is proposed. Since the proposed method can switch the desired signal space and the interference space of received signals, an additional selective diversity gain increases. The performance analysis using probabilistic models provides a mathematical expression for the sum-rate capacity. Simulation examples show that the proposed method achieves the higher sum-rate capacity than that of the conventional opportunistic interference alignment.

• International journal of advanced smart convergence
• /
• v.9 no.2
• /
• pp.58-67
• /
• 2020

In this paper, we propose a data-driven-based beam selection scheme for massive multiple-input and multiple-output (MIMO) systems in ultra-dense networks (UDN), which is capable of addressing the problem of high computational cost of conventional coordinated beamforming approaches. We consider highly dense small-cell scenarios with more small cells than mobile stations, in the millimetre-wave band. The analog beam selection for hybrid beamforming is a key issue in realizing millimetre-wave UDN MIMO systems. To reduce the computation complexity for the analog beam selection, in this paper, two deep neural network models are used. The channel samples, channel gains, and radio frequency beamforming vectors between the access points and mobile stations are collected at the central/cloud unit that is connected to all the small-cell access points, and are used to train the networks. The proposed machine-learning-based scheme provides an approach for the effective implementation of massive MIMO system in UDN environment.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.36 no.5A
• /
• pp.457-463
• /
• 2011

This paper proposes theorthogonal reference vectors selection method of the subspace interference alignment. The proposed method selects multiple orthogonal reference vectors instead of using one reference vector for all users at the same time. The proposed scheme selects a reference vector which maximizes a sum-rate for a certain cell, generates orthogonal vectors to the previous selected vector and selects the one of orthogonal vectors whose sum rate is maximized for each cell. Larger channel gain and sum-rate than the previous method can be obtained by selection degree of freedom. The computer simulation demonstrates the proposed method gives higher sum-rate compared with that of the previous reference vector selection method.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.19 no.6
• /
• pp.1307-1312
• /
• 2015

As smart-phones become popular, mobile data traffic has been dramatically increasing and intensive researches on the next-generation mobile communication network is in progress to meet the increasing demand for mobile data traffic. In particular, heterogeneous network (HetNet) is attracting much interest because it can significantly enhance the network capacity by increasing the spatial reuse with macro and small cells. In the HetNet, we have several problems such as load imbalance and interference because of the difference in transmit power between macro and small cells and cell range expansion (CRE) can mitigate the problems. In this paper, we propose a new cell selection scheme with adaptive cell range expansion bias (CREB) for ultra dense HetNet and we analyze the performance of the proposed scheme in terms of average cell transmission rate through system-level simulations and compare it with those of other schemes.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.05a
• /
• pp.63-66
• /
• 2015

As smart-phones become popular, mobile data traffic has been dramatically increasing and intensive researches on the next-generation mobile communication network is in progress to meet the increasing demand for mobile data traffic. In particular, heterogeneous network (HetNet) is attracting much interest because it can significantly enhance the network capacity by increasing the spatial resue with macro and small cells. In the HetNet, we have several problems such as load imbalance and interference because of the difference in transmit power between macro and small cells and cell range expansion (CRE) can mitigate the problems. In this paper, we propose a new cell selection scheme with adaptive cell range expansion bias (CREB) for ultra dense HetNet and we analyze the performance of the proposed scheme in terms of average cell transmission rate through system-level simulations and compare it with those of other schemes.