• 한국농공학회지
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• 제40권2호
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• pp.123-129
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• 1998

Compressibility and permeability properties are the most important input parameters necessary to assess the suitability of core materials in seepage control system construction. To achieve this objective, an experimental investigation was conducted in the laboratory. For the bentonite-soil/sand mixes, consolidation and permeability tests were carried out in the conventional consolidation cell, 6Omm in diameter and 2Omm in height, was modified to perform a falling head type permeability test. From the results, the normalized relationship with respect to void ratio at liquid-limit state $(e_L)$, and the changes of compressibility and permeability for various bentonite-soil/sand mixes were presented. This approach will be helpful in proportioning mixes and predicting corresponding changes in engineering behavior. And it is possible to proportion a mix to arrive at the required compressibility without affecting the permeability.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2002년도 추계학술발표대회 논문집
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• pp.68-70
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• 2002

Prediction of 3-D permeability tensor for multi-axial preform is critical to model and design the manufacturing process of composites by considering resin flow through the multi-axial fiber structure. In this study, the in-plane and transverse permeabilities for braided preform are predicted numerically. The flow analyses are calculated by using 3-D CVFEM(control volume finite element method) for macro-unit cells. To avoid checker-board pressure field and improve the efficiency of numerical computation, a new interpolation function for velocity is proposed on the basis of analytic solutions. Permeability of a braided preform is measured through unidirectional flow experiment and compared with the permeability calculated numerically. Unlike other studies, the current study is based on more realistic unit cell and prediction of permeability is improved.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2003년도 추계학술발표대회 논문집
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• pp.83-86
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• 2003

Complete prediction of second order permeability tensor for three dimensional preform such as plain woven fabric and braided preform is critical to understand the resin transfer molding process of composites. The permeability can be obtained by various methods such as analytic, numerical, and experimental methods. For several decades, the permeability has studied numerically to avoid practical difficulty of many experiments. However, the predicted permeabilities are a bit wrong compared with experimentally measured data. In this study, numerical calculation of permeability was conducted for two kinds of preforms i.e., plain woven fabric and circular braided preform. In order to consider intra-tow flow in the unit cell of preform the proposed flow coupled model was used for plain woven fabric and the Brinkman equation was solved in the case of the braided preform.

• Journal of Plant Biology
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• 제31권2호
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• pp.113-119
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• 1988

The effects of the defoliant and desiccant dimethipin(2,3-dihydro-5,6-dimethyl-1,4-dithiin-1,1,4,4-tetraoxide) on the passive permeability and osmotic ground value of onion(Allium cepa L.) epidermal cells were investigated by plasmometric method. The osmotic ground value and the water permeability of onion epidermal cells were decreased by 9.8% and 41.2%, respectively, and the passive permeabilities of nonelectrolytesurea, methylurea, ethylurea and malonamide-were also decreased by the range of 15.7%∼57.8% after treatment with 10-3M dimethipin. After treatment with 10-4M dimethipin, the osimotic ground value and the solute permeability of onion epidermal cells were also decreased by 3.7% and 24.5%∼48.8%, respectively, but the water permeability of onion epidermal cells was increased by 8.3%. It was suggested that dimethipin treatment modified the cell membrane of onion epidermis.

• Toxicological Research
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• 제11권1호
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• pp.103-108
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• 1995

Rat renal proximal tubule suspension was prepared from adult male Sprague Dawley rat (250-300g) by mechanical (non-enzymatical) method and evaluated as a pontential model for mechanistic studies and early screening of nephrotoxicity, using anionic antibiotics (cephaloridine). Cephaloridine (CPL) produced an increase in LDH release into media. This release results from decrease a proximal tubule cell viability and subsequently increase the permeability of cell viability and subsequently increase the permeability of cell membrane. Since loss of intracellular potassium and ATP into media is the sign of disruption of cell membrane, especially basolateral membrane (BLM), CPL induced proximal tubule cell compromise also appear be associated with BLM, maybe $Na^+-K^+$ ATPase. Also seen was significant depression in brush border membrane (BBM) ALP activity and no significantly increase in BBM GGT activities. The inhibition of typical anion, PAH accumulation (especially, CPL 5 mM) and cation, TEA (especially, 4hours incubation) were seen dose dependently. This is because of CPL accumulation in renal proximal tubule and increase of cytotoxicity.

• The Korean Journal of Physiology
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• 제23권1호
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• pp.13-21
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• 1989

The present study was designed to investigate i) the action of various nucleotides on membrane permeability of rat red blood cell and hepatocyte for $Na^{+}$ and $Rb^{+}$ ii) the characteristics of purinoceptors on these cell membranes. Blood from Sprague-Dawley rats was obtained by carotid arterial cannulation. Red blood cells were then washed 3 times with saline at $4{\circ}C$. Hepatic parenchymal cells were isolated from rat livers by using a modification of the Berry and Friend (1969) method. For the $Na^{+}$ influx studies, isolated RBC and hepatocyte were incubated in incubation medium containing $^{22}Na^{+}0.2\;{\mu}Ci/ml$ at $37^{\circ}C$. After various time intervals samples were removed from the incubation flask and washed out 3 times with ice-cold washing solutions. Cells were destroyed by adding Triton X-100 and TCA solution. After centrifugation, the supernatants were assayed for $^{22}Na^{+}$ by gamma counter. $^{86}Rb^{+}$ was used to simulate $K^{+}$ in these $K^{+}efflux$ studies. Isolated hepatocytes were incubated for 60 min in the loading solution containing $^{86}Rb^{+}\;10\;{\mu}Ci/ml$ at $37^{\circ}C$. After loading, the cells washed out 3 times by centrifugation with washing solution. The cells were incubated in buffer solution at $37^{\circ}C$. At intervals thereafter, samples were removed and centrifuged. The supernatants were analyzed for $^{86}Rb^{+}$ by liquid scintillation counter. The main results of the experiments were: 1) ATP and ATPP increased in both $^{22}Na^{+}$ influx and $^{86}Rb^{+}$ efflux in the red blood cell. Although ADP showed a tendency to increase in RBC membrane permeability for $^{22}Na^{+}$ and $^{86}Rb^{+}$, the changes were not significantly different from the control. 2) The Significant changes in $^{22}Na^{+}$ and $^{86}Rb^{+}$ flux by ATP were also demonstrated in hepatocyte. ATPP and ADP showed a tendency to increase in hepatocyte membrane permeability for both ions. 3) Other nucleoside triphosphates-ITP, GTP and CTP-did not change in membrane permeability for $^{22}Na^{+}$ and $^{86}Rb^{+}$ in RBC and hepatocyte. In conclusion, not only ATP but also ATPP activate purinoceptors and change in membrane permeability for $Na^{+}$ and $K^{+}$. In order to activate purinoceptors on the cell membrane, the nucleotides have to possess intact adenine moiety and three phosphates or more in its molecule.

• 한국지반환경공학회 논문집
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• 제14권7호
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• pp.51-56
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• 2013

최근, 시강우량의 최대치의 기록이 매년 바뀌고 있으며 이로 인해 도로의 침수피해 사례가 증가하고 있다. 이러한 이유로 새로운 배수포장체가 개발되고 있으나 보조기층에 대해서는 별도의 고려가 없다. 본 연구에서는 보조기층의 강도와 투수성을 함께 고려한 적정입도분포 기준을 제안하며, 이때 실내실험을 통해 각각의 강도특성 및 투수성을 비교하고자 한다. 실험에 앞서 국내외 보조기층 기준에 대한 시방기준을 토대로 4가지의 대표적인 입도분포를 산정하고 이를 실험하였다. 실험에 있어서 강도특성은 회복탄성계수 산정을 비교하고, 투수특성은 수직방향의 상향 및 하향 투수계수를 비교하였다. 회복탄성계수 산정은 $M_R$ 시험을 통하였으며, 이때 본 연구에서는 진동삼축시험기기를 보완하여 이를 구현하도록 하였다. 투수시험의 경우에는 2가지 투수시험이 수행되었으며, 하향의 흐름에 대한 투수시험으로는 기존의 변수위 투수시험이 적용되었고, 상향의 경우 직경 150mm의 중형 Rowe Cell 투수시험기를 이용하여 시험을 수행하였다. 연구결과, 회복탄성계수와 투수계수의 결과들을 종합하여 포장체 보조기층의 강도특성과 투수특성을 모두 만족할 수 있는 적정입도분포를 선정하고, 이를 통해 적정입도기준 마련이 가능할 것으로 판단된다.

• Biomolecules & Therapeutics
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• 제12권2호
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• pp.92-100
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• 2004

Effect of the depletion or oxidation of GSH on mitomycin c (MMC)-induced mitochondrial damage and cell death was assessed in small cell lung cancer (SCLC) cells. MMC induced cell death and the decrease in the GSH contents in SCLC cells, which were inhibited by z-LEHD.fmk (a cell permeable inhibitor of caspase-9), z-DQMD.fmk (a cell permeable inhibitor of caspase-3) and thiol compound, N-acetylcysteine. MMC caused nuclear damage, release of cytochrome c and activation of caspase-3, which were reduced by N-acetylcysteine. The depletion of GSH due to L-butionine-sulfoximine enhanced the MMC-induced cell death and formation of reactive oxygen species in SCLC cells, whereas the oxidation of GSH due to diamide or $NH_2Cl$ did not affect cytotoxicity of MMC. The results show that MMC may cause cell death in SCLC cells by inducing mitochondrial dysfunction, leading to activation of caspase-9 and -3. The MMC-induced change in the mitochondrial membrane permeability, followed by cell death, in SCLC cells may be significantly enhanced by the depletion of GSH. In contrast, the oxidation of GSH may not affect cytotoxicity of MMC.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.395-404
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• 2017

Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from Paeonia lactiflora against Candida albicans, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabling membrane depolarization, and compromising permeability. First, it was shown that the ethanol extract from P. lactiflora was involved in damaging the integrity of cell walls in C. albicans. In isotonic media, cell bursts of C. albicans by the P. lactiflora ethanol extract could be restored, and the minimum inhibitory concentration (MIC) of the P. lactiflora ethanol extract against C. albicans cells increased 4-fold. In addition, synthesis of $(1,3)-{\beta}-{\small{D}}-glucan$ polymer was inhibited by 87% and 83% following treatment of C. albicans microsomes with the P. lactiflora ethanol extract at their $1{\times}MIC$ and $2{\times}MIC$, respectively. Second, the ethanol extract from P. lactiflora influenced the function of C. albicans cell membranes. C. albicans cells treated with the P. lactiflora ethanol extract formed red aggregates by staining with a membrane-impermeable dye, propidium iodide. Membrane depolarization manifested as increased fluorescence intensity by staining P. lactiflora-treated C. albicans cells with a membrane-potential marker, $DiBAC_4(3)$ ((bis-1,3-dibutylbarbituric acid) trimethine oxonol). Membrane permeability was assessed by crystal violet assay, and C. albicans cells treated with the P. lactiflora ethanol extract exhibited significant uptake of crystal violet in a concentration-dependent manner. The findings suggest that P. lactiflora ethanol extract is a viable and effective candidate for the development of new antifungal agents to treat Candida-associated diseases.

• The Korean Journal of Physiology and Pharmacology
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• 제13권2호
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• pp.115-121
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• 2009

Functional defects in mitochondria are involved in the induction of cell death in cancer cells. We assessed the toxic effect of camptothecin against the human cervical and uterine tumor cell line SiHa with respect to the mitochondria-mediated cell death process, and examined the combined effect of camptothecin and anticancer drugs. Camptothecin caused apoptosis in SiHa cells by inducing mitochondrial membrane permeability changes that lead to the loss of mitochondrial membrane potential, decreased Bcl-2 levels, cytochrome c release, caspase-3 activation, formation of reactive oxygen species and depletion of GSH. Combination of camptothecin with other anticancer drugs (carboplatin, paclitaxel, doxorubicin and mitomycin c) or signaling inhibitors (farnesyltransferase inhibitor and ERK inhibitor) did not enhance the camptothecin-induced cell death and caspase-3 activation. These results suggest that camptothecin may cause cell death in SiHa cells by inducing changes in mitochondrial membrane permeability, which leads to cytochrome c release and activation of caspase-3. This effect is also associated with increased formation of reactive oxygen species and depletion of GSH. Combination with other anticancer drugs (or signaling inhibitors) does not appear to increase the anti-tumor effect of camptothecin against SiHa cells, but rather may reduce it. Combination of camptothecin with other anticancer drugs does not seem to provide a benefit in the treatment of cervical and uterine cancer compared with camptothecin monotherapy.