• Title/Summary/Keyword: Cell imaging

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FREE VIEWPOINT IMAGE RECONSTRUCTION FROM 3-D MULTI-FOCUS IMAGING SEQUENCES AND ITS IMPLEMENTATION BY CELL-BASED COMPUTING

  • Yonezawayz, Hiroki;Kodamay, Kazuya;Hamamotoz, Takayuki
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.751-754
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    • 2009
  • This paper deals with the Cell-based distributed processing for generating free viewpoint images by merging multiple differently focused images. We previously proposed the method of generating free viewpoint images without any depth estimation. However, it is not so easy to realize real-time image reconstruction based on our previous method. In this paper, we discuss the method to reduce the processing time by dimension reduction for image filtering and Cell-based distributed processing. Especially, the method of high-speed image reconstruction by the Cell processor on SONY PLAYSTATION3(PS3) is described in detail. We show some experimental results by using real images and we discuss the possibility of real-time free viewpoint image reconstruction.

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Current Status of Liquid-cell Transmission Electron Microscopy (액상 투과전자현미경 분석기법 소개 및 최신 연구동향)

  • Hong, Jaeyoung;Chun, Dong Won
    • Ceramist
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    • v.22 no.4
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    • pp.417-428
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    • 2019
  • Even though, nanoscale materials of various shapes and compositions have been synthesized in the liquid, their underlying growth and transformation mechanisms are not well understood due to a lack of analytical methods. The advent of liquid cell for transmission electron microscope (TEM) enables the direct imaging of chemical reactions that occur in liquids with nanometer resolution of the electron microscope (EM). Here, the technical development of liquid cell TEM equipment and their applications to the study of nanomaterials analysis in liquid are discussed. Also new findings discovered through liquid cell TEM studies such as nucleation & growth, coalescence process and transformation are discussed.

A Rice Blast Fungus Alpha-N-Arabinofuranosidase B Elicits Host Defense in Rice

  • Kim, Sun-Tae
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.23-23
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    • 2015
  • Rice blast disease caused by M. oryzae is the most devastating fungal disease in rice. During the infection process, M. oryzae secretes a large number of glycosyl hydrolase (GH) proteins into the apoplast to digest host cell wall and assist fungal ingress into host tissues. In this study, we identified a novel M. oryze arabinofuranosidase B (MoAbfB) which is secreted during fungal infection. Live-cell imaging exhibited that fluorescent labeled MoAbfB was highly accumulated in fungal invasive structures such as appressorium, tips of penetration peg, biotrophic interfacial complex (BIC), as well as invasive hyphal tip. Deletion of MoAbfB mutants extended biotrophic phase followed by enhanced disease severity, whereas, over-expression of OsMoAbfB mutant induced rapid defense responses and enhanced rice resistance to M. oryzae infection. Furthermore, exogenous treatment of MoAbfB protein showed inhibition of fungal infection via priming of defense gene expression. We later found that the extract of MoAbfB degraded rice cell wall fragments could also induce host defense activation, suggesting that not MoAbfB itself but oligosaccharides (OGs) derived from MoAbfB dissolved rice cell wall elicited rice innate immunity.

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Central giant cell lesion of the mandible in a 2-year old girl

  • Oda, Takaaki;Sue, Mikiko;Okada, Yasuo;Kanri, Yoriaki;Ono, Junya;Ogura, Ichiro
    • Imaging Science in Dentistry
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    • v.47 no.3
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    • pp.209-213
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    • 2017
  • Central giant cell lesions are rare, benign, osteolytic, pseudocystic, solitary, localized lesions that are common in the skeletal structure, but less so in the maxillofacial region. Furthermore, to perform panoramic radiography and cone-beam computed tomography, it is necessary to prepare patients properly and to position their heads carefully. However, this can be difficult in pediatric patients, who may be anxious. In this report, we describe the case of a central giant cell lesion of the mandible in a 2-year-old girl that was evaluated with multidetector computed tomography.

Automatic Detection of Forgery in Cell phone Images using Analysis of CFA Pattern Characteristics in Imaging Sensor (휴대폰의 CFA 패턴특성을 이용한 사진 위변조 탐지)

  • Shim, Jae-Youen;Kim, Seong-Whan
    • Proceedings of the Korea Information Processing Society Conference
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    • 2010.11a
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    • pp.1118-1121
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    • 2010
  • With the advent of cell phone digital cameras, and sophisticated photo editing software, digital images can be easily manipulated and altered. Although good forgeries may leave no visual clues of having been tampered with, they may, nevertheless, alter the underlying statistics of an image. Most digital camera equipped in cell phones employ a single image sensor in conjunction with a color filter array (CFA), and then interpolates the missing color samples to obtain a three channel color image. This interpolation introduces specific correlations which are likely to be destroyed when tampering with an image. We quantify the specific correlations introduced by CFA interpolation, and describe how these correlations, or lack thereof, can be automatically detected in any portion of an image. We show the efficacy of this approach in revealing traces of digital tampering in lossless and lossy compressed color images interpolated with several different CFA algorithms in test cell phones.

Development and Validation of a Model Using Radiomics Features from an Apparent Diffusion Coefficient Map to Diagnose Local Tumor Recurrence in Patients Treated for Head and Neck Squamous Cell Carcinoma

  • Minjae Kim;Jeong Hyun Lee;Leehi Joo;Boryeong Jeong;Seonok Kim;Sungwon Ham;Jihye Yun;NamKug Kim;Sae Rom Chung;Young Jun Choi;Jung Hwan Baek;Ji Ye Lee;Ji-hoon Kim
    • Korean Journal of Radiology
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    • v.23 no.11
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    • pp.1078-1088
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    • 2022
  • Objective: To develop and validate a model using radiomics features from apparent diffusion coefficient (ADC) map to diagnose local tumor recurrence in head and neck squamous cell carcinoma (HNSCC). Materials and Methods: This retrospective study included 285 patients (mean age ± standard deviation, 62 ± 12 years; 220 male, 77.2%), including 215 for training (n = 161) and internal validation (n = 54) and 70 others for external validation, with newly developed contrast-enhancing lesions at the primary cancer site on the surveillance MRI following definitive treatment of HNSCC between January 2014 and October 2019. Of the 215 and 70 patients, 127 and 34, respectively, had local tumor recurrence. Radiomics models using radiomics scores were created separately for T2-weighted imaging (T2WI), contrast-enhanced T1-weighted imaging (CE-T1WI), and ADC maps using non-zero coefficients from the least absolute shrinkage and selection operator in the training set. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic performance of each radiomics score and known clinical parameter (age, sex, and clinical stage) in the internal and external validation sets. Results: Five radiomics features from T2WI, six from CE-T1WI, and nine from ADC maps were selected and used to develop the respective radiomics models. The area under ROC curve (AUROC) of ADC radiomics score was 0.76 (95% confidence interval [CI], 0.62-0.89) and 0.77 (95% CI, 0.65-0.88) in the internal and external validation sets, respectively. These were significantly higher than the AUROC values of T2WI (0.53 [95% CI, 0.40-0.67], p = 0.006), CE-T1WI (0.53 [95% CI, 0.40-0.67], p = 0.012), and clinical parameters (0.53 [95% CI, 0.39-0.67], p = 0.021) in the external validation set. Conclusion: The radiomics model using ADC maps exhibited higher diagnostic performance than those of the radiomics models using T2WI or CE-T1WI and clinical parameters in the diagnosis of local tumor recurrence in HNSCC following definitive treatment.

Cavernous Sinus Metastasis of Non-Small Cell Lung Cancer

  • Ahn, Young;Yang, Jae-Hyun;Kim, Hyung-Jin;Jang, Sang-Eon;Jang, Young-Joo;Kim, Hye-Ryoun;Kim, Cheol-Hyeon;Choi, Sang-Yul;Lee, Jae-Cheol
    • Tuberculosis and Respiratory Diseases
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    • v.69 no.5
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    • pp.381-384
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    • 2010
  • Progressive ptosis and headache developed in a 50-year-old woman with non-small cell lung cancer. Although brain magnetic resonance imaging showed improved cerebellar metastasis after prior radiotherapy without any other abnormality, the follow-up examination taken 6 months later revealed metastasis to the cavernous sinus. The diagnosis of metastasis to the cavernous sinus is often difficult because it is a very rare manifestation of lung cancer, and symptoms can occur prior to developing a radiologically detectable lesion. Therefore, when a strong clinical suspicion of cavernous sinus metastasis exists, thorough neurologic examination and serial brain imaging should be followed up to avoid overlooking the lesion.

Behavior of Bacteria on the Porous Substrates: Diffusion Effect (다공성 매질 표면에서 박테리아의 거동: 확산의 영향)

  • Cho, Myoung-Ock;Cho, Ji-Yong;Park, Eun-Jung;Lee, Dong-Hee;Lee, Jeong-Hoon;Kim, Jung-Kyung
    • Journal of the Korean Society of Visualization
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    • v.6 no.2
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    • pp.45-50
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    • 2008
  • It has been found that the colony size of bacteria grown on an agar plate decreases with increasing agar gel concentration. Evidenc from recent studies suggests that the bacterial colony dynamics is closely related with the mechanical properties of the substrate. We investigate whether bacterial growth on the agar substrate is controlled mostly by the nutrients' diffusion which is hindered more in porous medium than in solution. The number of bacterial cells in single colonies is found to be inversely correlated with agar concentration. High-resolution live cell imaging at the single bacterium level confirms that the bacterial growth rate is reduced with increasing agar concentration. There is a strong correlation between the slowed diffusion and the reduced number of cells in a high concentration of agar medium.

2D ISAR Imaging using PFA and CDT Algorithms (PFA와 CDT 알고리즘을 이용한 2차원 ISAR 영상 생성)

  • Yoo Ji-Hee
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.15 no.9
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    • pp.906-913
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    • 2004
  • FFT algorithm is the most popular ISAR imaging technique from radar data. It requires polar formatting technique to make a focused image of the target as MTRC(Moving Through Resolution Cell) causes a blurred image when the data is from the wide azimuth angle. But there exits the angle limit for the application of the polar formatting and we cannot obtain clear images if the range of the azimuth angle is too wide to process with polar, formatting. This paper analyses the relative merits of the polar formatting algorithm accompanied by interpolation to the CDT algorithm that needs not the interpolation.

Imaging of Tumor Cell Proliferation using Radiofluorinated Ethyluracil and Deoxyadenosine (Radiofluorinated Ethyluracil과 Deoxyadenosine을 이용한 종양세포 증식의 영상화에 대한 연구)

  • Kim, Chang-Guhn;Yang, David J.;Kim, E. Edmund
    • The Korean Journal of Nuclear Medicine
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    • v.30 no.4
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    • pp.532-540
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    • 1996
  • 목적 : 종양세포의 증식을 평가하기 위해 radiofluorinated ethyluracil (FEU)과 deoxyadenosine analogue(FAD)를 합성하여 종양의 영상화를 시도하였다. 대상 및 방법 : 5-(2-Fluoroethyl)uracil ([$^{18}F$]FEU)은 2, 4-dimethoxy-5-(2-hydroxyethyl) pyrimidine을 $K^{18}F$와 처리한 후 HBr로 가수분해하여 얻었으며 Fluorodeoxyadenosine은 adenosine의 triacetylated analogue를 $K^{18}F$와 처리하여 얻었다. 생물학적 조직분포는 유방암 세포(13762 NF, 100,000 cells per rat, im)를 쥐에 접종한 후 0.5, 1, 2 및 4시간에 주요장기를 적출하여 %ID/g을 측정하고 자가방사영상은 방사성의약품 투여 45분 후에 얻었다. PET 영상은 VX-2 종양을 접종한 가토를 이용하여 얻었다. In vitro cell proliferation assay는 사람의 말초단핵구를 이용하였다. 결 과 : In vitro assay상 ([$^{18}F$]FEU는 세포증식시 DNA/RNA에 결합함을 시사하였다. ([$^{18}F$]FAD와 ([$^{18}F$]FEU의 종양/비종양 방사능 섭취비는 시간경과에 따라 증가하였으며 ([$^{18}F$]FAD와 ([$^{18}F$]FEU를 이용한 자가방사영상과 ([$^{18}F$]FEU를 이용한 PET 영상에서 종양을 잘 관찰할 수 있었다. 결 론 : ([$^{18}F$]FAD 및 ([$^{18}F$]FEU를 이용하여 종양세포의 증식을 PET 영상에서 평가할 수 있으리라 사료된다.

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