• Title/Summary/Keyword: Cell complex

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Electron Microscopic Studies of the Mouse Oocyte;Cumlus Complex in Vitro (인공배양한 생쥐 난자;난구복합체의 전자현미경적 연구)

  • Lee, Gy-Soog;Kim, Jong-Duk;Kwon, Hyuk-Bang
    • Clinical and Experimental Reproductive Medicine
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    • v.17 no.2
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    • pp.185-196
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    • 1990
  • These experiments were performed to know ultrastructural changes of the cumulus expansion in virot. SEM:In expanded oocyte-cumulus complex, the cell surface are characterized by the presence of many evaginations:they are relatively short and round shape. The mucous extracellular material were deposited between cumulus cells. TEM:In compact cumulus cells, golgi apparatus and rough endoplasmic reticulum developed. In expanded cumulus cells, rough endoplasmic reticulum decreased and the smooth endoplasmic reticulum increased. Also, there were numbers of mitochondria. Extracellular mucous material which is presumed to be hyaluronic acid appears when cumulus cell were expanded. In expanded cumulus cell, numbers of smooth endoplasmic reticulum help cumulus cell to develop in steroidogenic cell.

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Structural basis of Ca2+ uptake by mitochondrial calcium uniporter in mitochondria: a brief review

  • Jiho, Yoo
    • BMB Reports
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    • v.55 no.11
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    • pp.528-534
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    • 2022
  • Mitochondria are cellular organelles that perform various functions within cells. They are responsible for ATP production, cell-signal regulation, autophagy, and cell apoptosis. Because the mitochondrial proteins that perform these functions need Ca2+ ions for their activity, mitochondria have ion channels to selectively uptake Ca2+ ions from the cytoplasm. The ion channel known to play the most important role in the Ca2+ uptake in mitochondria is the mitochondrial calcium uniporter (MCU) holo-complex located in the inner mitochondrial membrane (IMM). This ion channel complex exists in the form of a complex consisting of the pore-forming protein through which the Ca2+ ions are transported into the mitochondrial matrix, and the auxiliary protein involved in regulating the activity of the Ca2+ uptake by the MCU holo-complex. Studies of this MCU holo-complex have long been conducted, but we didn't know in detail how mitochondria uptake Ca2+ ions through this ion channel complex or how the activity of this ion channel complex is regulated. Recently, the protein structure of the MCU holo-complex was identified, enabling the mechanism of Ca2+ uptake and its regulation by the MCU holo-complex to be confirmed. In this review, I will introduce the mechanism of action of the MCU holo-complex at the molecular level based on the Cryo-EM structure of the MCU holo-complex to help understand how mitochondria uptake the necessary Ca2+ ions through the MCU holo-complex and how these Ca2+ uptake mechanisms are regulated.

Targeting Cell-Cell and Cell-Matrix Interactions and Its Therapeutic Applications

  • Kim, In-San
    • Proceedings of the PSK Conference
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    • 2003.10a
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    • pp.100-101
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    • 2003
  • Cell-cell and cell-matrix interaction is clearly required for metazoans not only to hold their cells together but also to conduct more sophisticated biological processes. Each cell has adhesion molecules on its cell membrane to link extracellular matrix and adjacent cells to the intracellular cytoskeleton, and also to transduce signals. In complex metazoans, information is transmitted from one cell to another by mechanisms such as direct intercellular communication, soluble signal molecules among distant cells, and local cellular environments formed by highly specialized extracellular matrix. (omitted)

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Macrophage-Activating Factors Produced by Murine Leukemia X Fibroblast Hybrid Cells Stimulates Resistance to Mycobacterium avium Complex

  • Kim, Tae-Sung;Cohen, Edward-P.
    • Archives of Pharmacal Research
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    • v.20 no.3
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    • pp.225-233
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    • 1997
  • A murine leukemia x LM fibroblast hybrid cell line with immune augmenting properties stimulated resistance to Mycobacterium avium complex (MAC) in mouse peritoneal macrophages, and in immune deficient beige mice (C57BL/6/bgj/bgj). The proliferation of MAC in mouse peritoneal macrophages was inhibited by medium conditioned by the growth of the hybrid cells (hybrid cell-CM). Under similar circumstances, media conditioned by the growth of LM cells (LM cell-CM), a mouse fibroblast cell line used as one parent in forming the hybrid cell, was exhibited no inhibitory effect. Treatment of mouse peritoneal macrophages with hybrid cell-CM, but not with LM cell-CM, stimulated the expression of each of four previously described macrophage activation antigens, suggesting that the hybrid cells formed immunomodulators in addition to those formed by LM cells. Furthermore, the morphology of the macrophages following treatment with hybrid cell-CM was clearly distinguishable from that following exposure of the cells to LM cell-CM. The therapeutic effects of hybrid cells on the progression of MAC-infection were indicated by the prolonged survival of MAC-infected immune-deficient beige mice. One hundred percent of treated animals survived more than 60 days, while untreated animals died in approximately 22 days.

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Prediction of Water Quality and Water Treatment in Saemankeum Lake 3. Effects of Environmental Pollutants on Propagation of Freshwater Microalgae, Cryptomonas ovata and Feeding Rate of Corbicula leana (새만금호의 수질예측과 그에 따른 대책 3. 환경오염이 담수산 미세조류, Cryptomonas ovata의 증식과 참재첩(Corbicula leana) 섭이율에 미치는 영향)

  • 최문술;정의영;신윤경
    • The Korean Journal of Malacology
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    • v.14 no.2
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    • pp.167-172
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    • 1998
  • As a preliminary study, effects of environmetal pollutants on propagation of freshwater microalgae, Cryptomonas ovata and feeding rate of Corbicula leana were investigated at 20${\pm}$1$^{\circ}C$, over 20 days after treatment of pollutants, glucose, complex fertilizer and NH4Cl. Number of C. ovata in control group was increased from 38${\times}$104 cell/ml to 1.910${\times}$104 cell/ml after 20 days cultivation in Sorokin-Krauss medium. Increments of cell number in experimental groups treated with glucose, complex fertilizer and NH4Cl were higher than that of control group. The higher propagation rate of C.ovata was observed when 30 mg/l of glucose treated, 120 mg/l of complex fertilizer treated, and 4 mg/l of NH4Cl treated, compared with other concentrations in each pollutant treated group. The feeding rates of large size group of C. leana which fed with a living organism, C. ovata in each experimental group were higher than small size group, and slightly reduced with the increase of pollutant concentrations. The feeding rates were not significantly different between any concentrations of the pollutant, and among experimental groups treated with glucose, complex fertilizer and NH4Cl.

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Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

  • Kim, Sung Eun;Cho, Yong Woo;Kang, Eun Jung;Kwon, Ick Chan;Lee, Eunhee Bae;Kim, Jung Hyun;Chung, Hesson;Jeong, Seo Young
    • Fibers and Polymers
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    • v.2 no.2
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    • pp.64-70
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    • 2001
  • A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

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Regulation of SPIN90 by Cell Adhesion and ERK Activation

  • Kim Sung Hyun;Kim Dae Joong;Song Woo Keun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2004.05a
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    • pp.141-146
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    • 2004
  • SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

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Empirical Analysis on Rao-Scott First Order Adjustment for Two Population Homogeneity test Based on Stratified Three-Stage Cluster Sampling with PPS

  • Heo, Sunyeong
    • Journal of Integrative Natural Science
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    • v.7 no.3
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    • pp.208-213
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    • 2014
  • National-wide and/or large scale sample surveys generally use complex sample design. Traditional Pearson chi-square test is not appropriate for the categorical complex sample data. Rao-Scott suggested an adjustment method for Pearson chi-square test, which uses the average of eigenvalues of design matrix of cell probabilities. This study is to compare the efficiency of Rao-Scott first order adjusted test to Wald test for homogeneity between two populations using 2009 Gyeongnam regional education offices's customer satisfaction survey (2009 GREOCSS) data. The 2009 GREOCSS data were collected based on stratified three-stage cluster sampling with probability proportional to size. The empirical results show that the Rao-Scott adjusted test statistic using only the variances of cell probabilities is very close to the Wald test statistic, which uses the covariance matrix of cell probabilities, under the 2009 GREOCSS data based. However it is necessary to be cautious to use the Rao-Scott first order adjusted test statistic in the place of Wald test because its efficiency is decreasing as the relative variance of eigenvalues of the design matrix of cell probabilities is increasing, specially more when the number of degrees of freedom is small.

Autophagy in Cervical Cancer: An Emerging Therapeutic Target

  • Pandey, Saumya;Chandravati, Chandravati
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.4867-4871
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    • 2012
  • Cervical cancer is a leading cause of morbidity and mortality in women worldwide. Although the human papillomavirus (HPV) is considered the major causative agent of cervical cancer, yet the viral infection alone is not sufficient for cancer progression. The etiopathogenesis of cervical cancer is indeed complex; a precise understanding of the complex cellular/molecular mechanisms underlying the initiation, progression and/or prevention of the uterine cervix is therefore essential. Autophagy is emerging as an important biological mechanism in targeting human cancers, including cervical cancer. Furthermore, autophagy, a process of cytoplasm and cellular organelle degradation in lysosomes, has been implicated in homeostasis. Autophagic flux may vary depending on the cell/tissue type, thereby altering cell fate under stress conditions leading to cell survival and/or cell death. Autophagy may in turn govern tumor metastasis and subsequent carcinogenesis. Inflammation is a known hallmark of cancer. Vascular insufficiency in tumors, including cervical tissue, leads to depletion of glucose and/or oxygen perturbing the osmotic mileu causing extracellular acidosis in the tumor microenvironment that may eventually result in autophagy. Thus, targeted manipulation of complex autophagic signaling may prove to be an innovative strategy in identification of clinically relevant biomarkers in cervical cancer in the near future.