• Journal of Biomedical Engineering Research
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• v.39 no.4
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• pp.168-174
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• 2018

Adipocytes affect obesity through the regulation of lipid metabolism. Physical loading is an important regulator of fat tissue. There are ongoing in vitro studies inducing mechanotransduction on 3T3-L1 preadipocytes with mechanical stimulus in order to treat obesity by inhibiting adipogenesis and provoking cell death. In this study, our goal was to suggest a new therapy for obesity by investigating whether fluid shear stress (FSS) changes transcription factors on 3T3-L1 related with adipogenesis and cell death. FSS loading was applied to 3T3-L1 preadipocytes at 1Pa and 1Hz. After loading, bright field images were taken and an immunofluorescence assay was conducted to observe actin stress fiber formation. Western blot analysis was conducted to identify the activation of the ERK pathway as well as the adipogenic factors, which including C/EBPs and $PPAR{\gamma}$. The expression of osteopontin, a protein related to inflammation in adipose tissue, and cell death related factors, Bax, Bcl-2, and Beclin, were also measured. Results showed that FSS stimulated the formation of actin stress fibers in 3T3-L1 and also that the activation of C/EBPs decreased significantly when compared with the control group. $PPAR{\gamma}$ activation in the 2 hour FSS group was lower than the 1 hour FSS group, which implied that the results were time dependent. Additionally, there were no differences in the expression of cell death factors after FSS loading. In summary, similar to other fibroblasts, the formation of actin stress fibers induced by mechanotransduction may affect the differentiation of 3T3-L1, leading to inhibition of adipogenesis and inflammation.

• Journal of Industrial Technology
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• v.12
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• pp.25-36
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• 1992

In this paper, we simulate and calculate the stress and output voltage of the beam structure load cells by using FEM as varing physical structure parameters and loading positions. It is proved that stress enhance as the increase of the notch pitch and radius of the load cell, but decrease as the increase of the notch thickness and beam width. The results are good matched for basic formulas of the single fixed beam, and are verified our simulation is correct. Also, it is found that the stress characteristics of the load cell is varied according to loading positions with structure parameters, and caculated output voltage of the load cell approximate to those of the real manufactured ones. As a result, this study will offer efficient design and analysis technique for making special and variety capacity of load cells.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.22 no.10
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• pp.672-678
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• 2010

The purpose of this study is to develop a stacking guidance system (SGS) of containers in the mobile harbor (MH). A mobile harbor is a floating structure especially designed for loading and unloading containers from and to a large container ship. A novel stacking guidance system was proposed for unloading the container in an effective way against possible vibrations of the floating body. The guidance system works as an aid for loading containers with a wider opening for easier stacking of a container into a moving storage cell due to waves. In order to determine the most effective inclination angle of the cell-guide, this study performed the dynamic analysis of the SGS equipped in the MH subject to fluctuations of the sea. The motions of the guidance system and a container loaded were calculated using ADAMS. The simulation results of the contact force between the two rigid bodies showed that a desirable angle of the cell-guide should be around 20 degrees from the vertical. This proposed SGS can considerably reduce the loading and unloading time, and will enhance the performance of the MH.

• Structural Engineering and Mechanics
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• v.54 no.6
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• pp.1245-1266
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• 2015

In this research, the cylindrical absorber made of expanded metal sheets under impact loading has been examined. Expanded metal sheets due to their low weight, effective collapse mechanism has a high energy absorption capacity. Two types of absorbers with different cells angle were examined. First, the absorber with cell angle ${\alpha}=0$ and then the absorber with angle cell ${\alpha}=90$. Experimental Study is done by drop Hammer device and numerical investigation is done by finite element of ABAQUS software. The output of device is acceleration-time Diagram which is shown by Accelerometer that is located on the picky mass. Also the output of ABAQUS software is shown by force-displacement diagram. In this research, the numerical and experimental study of the collapse type, force-displacement diagrams and effective parameters has been investigated. Similarly, the comparison between numerical and experimental results has been observed that these results are matched well with each other. From the obtained results it was observed that the absorber with cell angle ${\alpha}=0$, have symmetric collapse and had high energy absorption capacity but the absorber with cell angle ${\alpha}=90$, had global buckling and the energy absorption value was not suitable.

• BMB Reports
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• v.49 no.11
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• pp.585-586
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• 2016

Extracellular vesicles (EVs) are natural carriers of biomolecules that play central roles in cell-to-cell communications. Based on this, there have been various attempts to use EVs as therapeutic drug carriers. From chemical reagents to nucleic acids, various macromolecules were successfully loaded into EVs; however, loading of proteins with high molecular weight has been huddled with several problems. Purification of recombinant proteins is expensive and time consuming, and easily results in modification of proteins due to physical or chemical forces. Also, the loading efficiency of conventional methods is too low for most proteins. We have recently proposed a new method, the so-called exosomes for protein loading via optically reversible protein-protein interaction (EXPLORs), to overcome the limitations. Since EXPLORs are produced by actively loading of intracellular proteins into EVs using blue light without protein purification steps, we demonstrated that the EXPLOR technique significantly improves the loading and delivery efficiency of therapeutic proteins. In further in vitro and in vivo experiments, we demonstrate the potential of EXPLOR technology as a novel platform for biopharmaceuticals, by successful delivery of several functional proteins such as Cre recombinase, into the target cells.

• Journal of the Korea Concrete Institute
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• v.11 no.3
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• pp.171-179
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• 1999

The present research investigated the interaction among loading level, corrosion rate and flexural deflection of reinforced concrete beams. 10cm$\times$15cm$\times$110cm reinforced concrete beams were prepared and subjected to different levels of flexural loading, including 0%, 45% and 75% of the ultimate load. The beams with either a pre-load or a sustained load were also exposed to a laboratory environment with ponding and wetting/drying cycling at room temperature. Half cell potential and galvanized current measurements were taken to monitor corrosion process of reinforcing steel. After corrosion initiation, external current was applied to some of the beams to accelerate corrosion propagation. The beam deflections were recorded during the entire tests. The results indicate that loading level has significant effect on corrosion rate. The beams under a sustained load had much higher corrosion rate than the pre-loaded and then unloaded beams. Significant corrosion may result in an increase in beam deflection and affect serviceability of the structure. The present research may provide an insight into structural condition evaluation and service life predictions of reinforced concrete.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.8A
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• pp.1223-1230
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• 2000

In this paper, we proposed a handoff decision method based on signal-to-interference ratio(SIR) of the pilot channel in order to perform a handoff more effectively and to complement disadvantages - deterioration quality of a call, decreasing capacity of the system, and wasting power of the mobile station - which is caused when handoff is performed by the classical method that execute a handoff based on received signal strength. Moreover, when we change that the minimum threshold, the cell loading which is defined active traffic channels to total traffic channels ratio, and the fraction of the transmit power from base station allocated to the pilot channel on the forward link of a DS-CDMA system, we analyzed mean numbers of handoff depending on hysteresis level during the mobile station moving from one base station to another base station.

• Journal of Environmental Health Sciences
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• v.22 no.3
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• pp.98-102
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• 1996

This study investigated performance of the phenol degradation and reaction characteristics according to variation of phenol volumetric loading rates and dilution rates in suspension and PACT reactors using Pseudomonas sp. B3. 1. Removal efficiencies of the PAC unit indicated about 100 % with phenol volumetric loading rates from 0.4 phenol $kg/ma^3\cdot d$ to 1.2 phenol $kg/m^3\cdot d$, however, which of the suspension reactor showed about 100% with from 0.2 phenol $kg/m^3\cdot d$ to 0.75 phenol $kg/ma^3\cdot day$. 2. The cell density slightly was decreased from 298.2 mg/l to 272 mg/l, when dilution rate for suspension was reactor increased from 0.4 to 1.41 1/d, and also the cell density suddenly was decreased to 145.5 mg/l and was washed out at the dilution rate higher than 1.60 1/d. But the cell density for the PAC unit was linearly decreased with dilution rate of from 0.8 to 3.0 1/d, and showed 220.75 mg/l at maximum dilution rate. 3. The phenol utilization rate was increased from 0.008 to 0.031 phenol g/l$\cdot$h, when dilution rate for suspension reactor was increased from 0.4 to 1.5 1/d, however, the rate for the PAC unit was linearly increased from 0.017 to 0.061 phenol g/l$\cdot$h as variation changes from 0.017 to 0.061 phenol g/l$\cdot$h dilution rate.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.29-32
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• 2008

The cell performance of direct formic acid fuel cell (DFAFC) having catalysts coated by electrospray was analyzed. Pd catalyst was used for the anode electrode and Pd catalyst loading amount and formic acid feed rate dependances of fuel cell performance were evaluated. When loading amount of Pd is in between 3mg/$cm^2$ and 7mg/$cm^2$ and formic acid feed rate is 5ml/min., 3mg/$cm^2$ sample showed better potential at 129 mA/$cm^2$ and power density due to difference in mass transfer limitation. However, when the feed rate is greater than 10ml/min., the opposite tendency was observed between 3mg/$cm^2$ and 7mg/$cm^2$ samples. The result was attributed to improvement in electrochemical reaction of the Pd. Based on the above results, In DFAFC including Pd catalyst that was coated by electrospray, 0.537V as the maximum potential at 129 mA/$cm^2$ was attained.

• Korean Journal of Ecology and Environment
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• v.44 no.4
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• pp.337-346
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• 2011

We investigated nutrient loading and trophic states in a coastal estuarine system in the Asan estuary by assessing phytoplankton biomass and using the trophic index (TRIX). The monthly and yearly nutrient loading (TN, TP) from freshwater discharge from the Asan and Sapgyo reservoirs into the estuary were estimated and analyzed with related factors. Monitoring data (physio-chemical and biological variables) collected at five estuary stations were used to assess trophic states. Descriptive statistics of total phytoplankton cells, chl a concentrations and primary productivity were also used to assess seasonal trophic status. N loading from freshwater ranged $1.0{\sim}1.3{\times}10^4$ ton yearly. The yearly P loading ranged between 350 and 400 ton during 2004~2006, increasing to 570 ton in 2007. Regression results suggest that DIN and DSi were correlated with freshwater discharge at the upper region. Based on phytoplankton biomass and total cell abundance, the trophic state of the estuary was found to be eutrophic during spring due to phytoplankton bloom. Primary productivity level was remarkably high, especially in summer coinciding with high nutrient loading. Pheopigments increased during warm seasons, i.e. summer and fall. Trophic index results indicate that the trophic state varied between mesotrophic and eutrophic in the estuary water body, especially in the upper region. The results suggest that phytoplankton production was regulated by nutrient loading from freshwater whereas biomass was affected by other properties than nutrient loading in the Asan Estuary ecosystem.