• 제목/요약/키워드: Cell Loading

검색결과 482건 처리시간 0.023초

Entrapped Mixed Microbial Cell (EMMC) 공정을 이용한 수산물 가공 폐수처리에서 유기물 및 질소 동시제거 (Simultaneous Removal of Organic and Nitrogen in the Treatment of Fish Processing Wastewater using Entrapped Mixed Microbial Cell (EMMC) Process)

  • 정병철;박권삼;정병곤
    • 한국물환경학회지
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    • 제22권3호
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    • pp.492-497
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    • 2006
  • Feasibility of simultaneous removal of organic materials and nitrogen in the wastewater from fisheries processing plant was evaluated using entrapped mixed microbial cell (EMMC) process. The experiment was performed using activated sludge from municipal sewage treatment plant which was immobilized with gel matrix by cellulose triacetate. It was found the stable operation at the treatment system which is composed of anoxic and oxic tank, was possible when the organic and nitrogen loading rates were increased stepwise. The organic and nitrogen loading rates were conducted from 0.65 to $1.72kgCOD/m^3/d$ and from 0.119 to $0.317kg\;T-N/m^3/d$ with four steps, respectively. The maximum nitrogen loading rate which could satisfy the regulated effluent standard of nitrogen concentration, was $0.3kg\;T-N/m^3/d$. The removal efficiency of total nitrogen was decreased apparently as increasing nitrogen loading rates, whereas the removal efficiency of ammonium nitrogen was effective at the all tested nitrogen loading rates. Therefore, it was concluded that nitrification was efficient at the system. Nitrate was removed efficiently at the anoxic tank. whereas the nitrification efficiency at the oxic tank ranged 94.0% to 96.9% at the tested loading rates. The removal efficiencies of chemical oxygen demand (COD) and those of total nitrogen at the entire system ranged from 94.2% to 96.6% and 73.4% to 83.4%, respectively.

Load cell 중량센서의 FEM simulation (FEM simulations of load cell weighing sensor)

  • 박찬원
    • E2M - 전기 전자와 첨단 소재
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    • 제7권3호
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    • pp.200-205
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    • 1994
  • The FEM technique was applied to simulate the change of stress characteristics for various structural parameters and loading positions of the load cell. The output voltage of the load cell was then computed to compare with the manufactured load cell. The tendency of the stress variations of the load cell was well agreed with the basic formula of the single fixed. beam. Also, the stress characteristics according to the change of loading positions showed respective featured results as different structure. The calculated output voltages of the load cell were very close to those of the real manufactured ones.

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신경전달물질 및 물리적 자극에 대한 뼈 세포의 반응 (Bone Cell Response to Neurotransmitters and Mechanical Loading)

  • 곽지현;김병관;김경환;김지현
    • 대한의용생체공학회:의공학회지
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    • 제30권1호
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    • pp.89-93
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    • 2009
  • Bone remodeling is a continuous process of skeletal renewal during which bone formation is tightly coupled to bone resorption. Mechanical loading is an important regulator of bone formation and resorption. In recent studies, neurotransmitters such as vasoactive intestinal peptide (VIP) were found to be present inside bone tissue and have been suggested to potentially regulate bone remodeling. In this study, our objective was to use a pre-established in vitro oscillatory fluid flow-induced shear stress mechanical loading system to quantify the effect of VIP on bone resorptive activity and investigate its combined effect with mechanical loading. VIP decreased osteoclastogenesis significantly decreased RANKL/OPG mRNA ration by approximately 90%. Combined VIP and mechanical loading further decreased RANKL/OPG ratio to approximately 95%. These results suggest that VIP present in bone tissue may synergistically act with mechanical loading to regulate bone remodeling via suppression of bone resorptive activities.

자동차 연료전지 진단 및 백금 촉매제 최소화에 대한 연구 (Methods for Diagnosis and Minimum PT-Loading of PEM Fuel Cell Stack)

  • 유승열;이용현
    • 전기학회논문지
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    • 제62권1호
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    • pp.105-108
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    • 2013
  • To resolve global environmental problems, many automobile companies are making a great deal of effort to develop so-called green-cars. One of the solutions is an electric vehicle equipped with the PEMFC (Proton Exchange Membrane Fuel Cell) stack. However, the cost of the stack, at the moment, is still too high. This obstacle must be resolved for commercialized fuel cell cars. One of the reasons for high cost is a relatively large amount of Pt (platinum) in the membrane. In this paper, a method for reducing the Pt-loading is introduced. Furthermore, the durability of the stack will be important for a company to stay competitive in world markets (in the future). For this, the diagnosis of the stack must be conducted on-line. Some diagnosis methods as key technology are also introduced in this paper.

고정화 공생 Bacteria를 이용한 호발폐수 중 PVA분해 신공정 개발 (Development of a New Process for PVA Degradation in Desize Wastewater by lmmobilized Symbiotic Bacteria)

  • 김정목;조무환조윤래정선용
    • KSBB Journal
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    • 제6권4호
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    • pp.395-402
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    • 1991
  • A new process was developed to degrade PVA(polyvinyl alcohol) in desize wastewater. Two symbiotic bacteria of Pseudomonas strain G5Y and PW were immobilized on the media by adsorption. A natural zeolite was chosen as the best media considering cell adhesion capacity, sedimentation rate, and material cost. PVA and COD removal efficiencies of this system for synthetic wastewater were 84% and 85% at the retention time of 6 hr, when the volumetric loading rate was PVA 8g/L·day and COD 8g/L·day, and cell density was 19,775 mg/L. In case of desire wastewater, they were 78% and 72% at the retention time of 6 hr, respectively, when the volumetric loading rate was PVA 8g/L·day and COD 13.2g/L·day, and cell density was 32,899mg/L. In case of desize wastewater, PVA and COD removal efficiencies were lower than synthetic wastewater, but cell density of the desize wastewater was lower than that of the synthetic wastewater, because there were insufficiency of necessary nutrition and variety of desize materials in the desize wastewater. A pilot test was successfully performed showing 88% and 82%, PVA and COD removal efficiencies at the retention time of 24 hr, when volumetric loading rate were 4.7 PVA g/L·day and COD 6.9g/L·day, and cell density was 12,,324 mg/L.

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Improved Cryopreservation Using Droplet-vitrification and Histological Changes Associated with Cryopreservation of Madder (Rubia akane Nakai)

  • Yi, Jung-Yoon;Sylvestre, Isabelle;Colin, Myriam;Salma, Mohammad;Lee, Sok-Young;Kim, Haeng-Hoon;Park, Hong-Jae;Engelmann, Florent
    • 원예과학기술지
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    • 제30권1호
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    • pp.79-84
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    • 2012
  • An efficient protocol for cryopreservation of madder hairy root cultures has been developed using droplet-vitrification. In previous study, combining loading solution C4 (35% PVS3) and vitrification solution B5 (80% PVS3) was the most effective method. In this study, we tried three types of vitrification solution, B5, A3 (90% PVS2, on ice), and A5 (70% PVS2, on ice). Combining loading solution C4 and vitrification solution A5 (on ice) showed the best regeneration rate in this study. Histological changes of the cells within the hairy root of madder were also observed in different steps. The cells from the hairy roots of the control treatment were full and intact with different size of vacuoles and obvious cell nucleus having a dark nucleolus. After the stage of preparing for cryopreservation (after preculturing, loading, followed by dehydration solution A5 or B5), intercellular spaces had become distinct, and within cells, the cytoplasms had become denser and week plasmolyses had appeared. The cell plasmolyses were much more apparent and we measured the degree of plasmolysis by calculating, the area of cell/the area of cytoplasm. The value of plasmolysis degree was the highest in the combination of preculture, loading solution C4, and dehydration solution A5, 1.97. Because the highest regeneration rates appeared in the treatment of A5 for 20 min, we could assume that the optimal degree of plasmolysis for cryopreservation might be around 1.97. The changes in cell structure during cryopreservation might be a useful basis for the development of a proper long-term preservation method for madder germplasms.

The Relationship Between Hydrogen Trapping Behavior and SSCC Suceptibility of API X60/65 Grade Steels

  • Lee, Jae Myung;Kim, Jin Suk;Kim, Kyoo Young
    • Corrosion Science and Technology
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    • 제2권3호
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    • pp.109-116
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    • 2003
  • It is well known that SSCC (sulfide stress corrosion cracking) is caused by drastic ingression of hydrogen during the service and accumulation of hydrogen near the potential crack initiation site in the material. It is important to characterize the hydrogen trapping behavior to evaluate the service performance of the high strength pipeline steels. In this study. the relationship between the hydrogen trapping behavior and SSCC susceptibility is evaluated in terms of alloy composition, microstructure and carbide behavior. The hydrogen trapping behavior was measured by electrochemical hydrogen permeation test cell (Devanathan cell). The SSCC susceptibility is evaluated by constant extension rate test and constant strain lest method. The hydrogen trapping behavior is affected greatly by microstructure and nature of carbide particles. The fine TiC, and NbC in the matrix of ferritic structure acts as strong irreversible trap sites whereas the bainitic structure acts as reversible trap site. The SSCC susceptibility is closely related to not only the hydrogen trapping behavior but also the loading condition. As the activity of reversible trap site increases, SSCC susceptibility decreases under static loading condition below yield strength, whereas SSCC susceptibility increases under dynamic loading condition or above yield strength. As the activity of irreversible trap site increases. SSCC susceptibility increases regardless of loading condition. It is cased by the mixed effect of dislocation on hydrogen diffusion and trapping behavior.

EFFECT OF INLET LOADING RATE ON THE ELIMINATION OF HYDROGEN SULFIDE AND AMMONIA IN IMMOBILIZED CELL BIOFILTERS

  • Kim, Jung-Hoon;Rene, Eldon R.;Park, Seung-Han;Park, Hung-Suck
    • Environmental Engineering Research
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    • 제11권5호
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    • pp.285-291
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    • 2006
  • Biofiltration is a simple, effective, economically viable and the most widely used gas treatment technique for treating malodors at low concentrations and high flow rates. This paper reports the performance of two lab scale immobilized cell biofilters operated in continuous mode for hydrogen sulfide ($H_2S$) and ammonia ($NH_3$) removal. The removal efficiency (RE, %) and the elimination capacity (EC, $g/m^3{\cdot}hr$) profiles were monitored by subjecting the biofilters to different loading rates of $H_2S$ (0.3 to $8\;g/m^3{\cdot}hr$) and $NH_3$ (0.3 to $4.5\;g/m^3{\cdot}hr$). The removal efficiencies were greater than 99% when inlet loading rate to the biofilters were upto $6\;gH_2S/m^3{\cdot}hr$ and $4\;gNH_3/m^3{\cdot}hr$ respectively. The performance of the biofilters were also ascertained by conducting shock loading studies at a loading rate of $10\;gH_2S/m^3{\cdot}hr$ and $6\;gNH_3/m^3{\cdot}hr$. The results from this study show high removal efficiency, good recuperating potential and stability of the immobilized microbial consortia to transient shock loads.