• 한국미생물·생명공학회지
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• 제24권6호
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• pp.733-737
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• 1996

The formation of microbial films(biofilm) by a non-sulfur phototrophic bacteria, Rhodopseudomonas capsulata, on inorganic media was studied. Porous ceramic beads(PCB) were superior to other immobilizing media for the biofilm formation in a packed-bed reactor. It was found that the formation of microbial films favored a lower hydraulic retention time, showing a higher ratio of cells attatched to the media to those suspended in the solution. The cell concentration in the biofilm reactor was as high as 11,400mg/l, which is 8-folds of the cell concentration in an ordinary suspended treatment. It was observed that the formation of micribial film by R. capsulata followed a general serial process of cell attachment, microcolony formation, and biofilm formation. The microbial films thus formed was very stable even for an extremely high volumetric BOD loading rate of 15gBOD/l day. The scanning electron micrographs of the microbial films showed that the cells were attached to both the surface and pores of the media.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 가을 학술발표회 논문집
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• pp.369-376
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• 2000

Parameters that represent the characteristic of consolidation have been determined by the oedometer tests. They are varied by the process of sampling and transportation, accuracy of apparatus, process of sample trimming and the skill of engineers. Also, it is difficult to grasp what factor exert a influence on the parameters. In this study, Slurry Rowe Cell Tests were peformed to evaluate the disturbance. Using the plot of e - log p which obtained from Rowe Cell test results, we compared the swelling indexes at the stress relief and the last loading stage. With that result, we tried to presume the disturbance of the 330 oedometer tests. The plot of compression-swelling index at the stress relief considerably scattered but at the last step of load shows a correlations between them. And the plot of depth-p$\sub$c/ shows a correlation with the different swelling indexes, but not in the LL-p$\sub$c/. Consequently, it is considered that the disturbance of the consolidation tests can be presumed by the comparison of the difference of swelling index.

• 전기화학회지
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• 제2권3호
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• pp.171-175
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• 1999

Novel process for high power catalyst electrode for PEM fuel cell has been developed. MEA having this catalyst electrode showed $0.5W/cm^2\;with\;0.2mg/cm^2$ of Pt loading at aunospheric humid hydrogen and oxygen condition. In this process, platinized carbon and plain carbon powders were coated with ionomer (Nafion) and hydrophobic polymer (PTFE), respectively and it could maximize two roles of catalyst electrode, l.e., reaction and gas supplying component. Those polarization characteristics proved the improved performance by reducing potential drop especially in the concentration polarization region.

• International Journal of Aeronautical and Space Sciences
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• 제10권2호
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• pp.95-105
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• 2009

The flow fields around the HARTII rotor were numerically investigated using a viscous flow solver on adaptive unstructured meshes. An overset mesh and a deforming mesh technique were used to handle the blade motion including blade deflection, which was obtain from the HARTII experimental data. A solution-adaptive mesh refinement technique was also used to capture the rotor wake effectively. Comparison of the sectional normal force and pitching moment at 87% radial station between the two cases, with and without the blade deflection, showed that the blade loading is significantly affected by blade torsion. It was found that as the mesh was refined, the strength of tip vortex is better preserved, and the magnitude of high frequency blade loading, caused by blade-vortex interaction (BVI), is further magnified. It was also found that a proper time step size, which corresponds to the cell size, should be used to predict unsteady solutions accurately. In general, the numerical results in terms of the unsteady blade loading and the rotor wake show good agreement with the experimental data.

• 한국세라믹학회지
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• 제44권10호
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• pp.574-579
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• 2007

Porous $Al_2O_3$ ceramics were prepared by the gelcasting foams method (a slurry foaming process) with acrylamide monomer. The foaming and gelation behavior was investigated with the parameters such as the type and concentration of surfactant, solid loading of slurry, and the concentrations of initiator and catalyst. Density, porosity, microstructure, and strength of the green and sintered samples were characterized. Of the four kinds of surfactants tested, Triton X-114 showed the highest foaming ability for the solid loading of 55-30 vol%. The gelation condition giving the idle time off min was found to set the foamed structure without significant bubble enlargement and liquid lamella thinning. The green samples were fairly strong and machinable and showed maximum strength of 2.4 MPa in diametral compression. The sintered samples showed densities of 10-36% theoretical (i.e. porosity 90-64%) with a highly interconnected network of spherical pores with sizes ranging from 30 to $600{\mu}m$. The pore size and connectivity increased but the cell strut thickness decreased with decreasing the solid loading. Flexural strength of 37.8-1.7 MPa was obtained for the sintered samples.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.103-103
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• 1993

목적: Estroge 결핍으로 나타나는 폐경기의 주요 증상을 경감 치료하기 위해, estrogen경구 투여시 위장장애, 위장에서의 대사, 간 초회효과, 자주 투여로 인한 환자의 불편 등의 단점이 있나, 이러한 점을 개선하기 위한 투여경로의 하나로 피부를 통해 일정약물을 장기간 일정속도로 송달시키는 경피 흡수 시스템의 개발이 필요하다. 따라서 (EE)의 장기제어방출을 위해 ethylene vinyl acetate (EVA)를 사용하여 장기 제어방출 및 경피 흡수의 최적화 조건을 설정하여 경피흡수 시스템을 위한 막의 개방을 목적으로 한다. 방법: VA 함량이 18-40％까지의 EVA를 사용하여 EE를 함유한 matrix를 casting 방법으로 제조하고 변형된 Keshary-Chien cell을 이용하여 방출실험을 실행하였다. 이때 방출에 미치는 여러 가지 인자로서 EVA 주의 VA함량, 막의 두께, receptor 중 PEG 400의 용량비율, 방출 매개체의 온도, loading 된 약물의 량 등에 대해 검토하였다. 그리고 점개한 mouse skin에 대한 투과 실험을 행하고 이에 미치는 PEG 400과 자질층의 역할을 검토하였다. 결과: EE의 용해도는 saline so PEG 400의 용량 비율이 증가함에 따라 지수 함수적으로 증가하였다. 그리고 VA 함량이 증가될수록, PEG 400의 용량비율, 방출 매개체의 온도, loading 된 약물의 양이 증가될수록 PEG 400의 용량비율, 방출 매개체의 온도, loading 된 약물의 양이 증가될수록 EE의 방출속도와 부과속도는 증가하였다. 또한 투과속도는 막두께의 역수와 직선상의 상관 관계를 보였다. 그리고 EVA matrix로부터 EE가 방출되는 양상은 diffusion-controlled model을 따랐으며 이때 단위면적당 방출된 총량은 T에 비례되었다. 절개한 mouse skin을 통한 EE의 permeation은 PEG 400의 첨가에 의해 상승되었다. 이와 같이 EVA 막이 EE의 부과 및 방출을 조절하는 것으로 보아 경피 흡수를 위해 사용될 수 있다고 사료된다.

• 한국자동차공학회논문집
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• 제19권6호
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• pp.10-16
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• 2011

Diesel particulate filter is being recognized that it is the most effective technologies to reduce particulate matter. In this study, to determine the characteristics of the cell-open-type pDPF, we employed p-DPF to exhaust gas tunnel of diesel engine and surveyed filtration efficiency and BPT on the basis of PM which is exhausted from engine. In this paper the soot loading mass in DPF can be predicted from increase of differential pressure of DPF so that we can measure filtration efficiency and Balance Point Temperature (BPT) by soot loading mass. The result of the research showed that the filtration efficiency is 65% in ESC mode with 0.7mm hole diameter. For the results of the characteristics of filtration efficiency and BPT according to mass_exh, we found that if mass_exh increases, filtration efficiency increases and BPT decreases.

• Archives of Pharmacal Research
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• 제28권8호
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• pp.988-994
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• 2005

The purpose of the present study was to prepare multivesicular liposomes with a high drug loading capacity and to investigate its potential applicability in the oral delivery of a peptide, human epidermal growth factor (rhEGF). The multivesicular liposomes containing rhEGF was prepared by a two-step water-in-oil-in-water double emulsification process. The loading efficiency was increased as rhEGF concentration increased from 1 to 5mg/mL, reaching approximately $60\%$ at 5 mg/mL. Approximately $47\%$ and $35\%$ of rhEGF was released from the multivesicular liposomes within 6 h in simulated intra-gastric fluid (pH 1.2) and intra-intestinal fluid (pH 7.4), respectively. rhEGF-loaded multivesicular liposomes markedly suppressed the enzymatic degradation of the peptide in an incubation with the Caco-2 cell homogenate. However, the transport of rhEGF from the multivesicular liposomes to the basolateral side of Caco­2 cells was two times lower than that of the rhEGF in aqueous solution. The gastric ulcer healing effect of rhEGF-loaded multivesicular liposomes was significantly enhanced compared with that of rhEGF in aqueous solution; the healing effect of the liposomes was comparable to that of the cimetidine in rats. Collectively, these results indicate that rhEGF-loaded multivesicular liposomes may be used as a new strategy for the development of an oral delivery system in the treatment of peptic ulcer diseases.

• 한국균학회지
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• 제23권4호통권75호
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• pp.354-358
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• 1995

용존 유기물의 농도를 BOD로 신속히 측정하여 폐수처리 공정에 feed back할 수 있는 Yeast BOD sensor 를 제작하여 BOD 측정의 거동을 조사하였다. BOD sensor의 감응도는 yeast의 활성이 가장 높은 pH 7.0 및 온도 $30^{\circ}C$에서 가장 높게 나타났다. Sensor에 충전되는 효모의 양도 감응도에 영향을 미치며 투석막 단위면적당 $ 0.14\;mg/cm^2$가 최적의 균체량이었다. 오염물질의 종류에 따라 BOD sensor의 출력 신호가 다르게 나타났으며 각각의 오염물질 속에 효모균를 전처리 시킨 후 sensor에 충전할 경우 sensor 의 감응도가 크게 증가하였다.

• 대한기계학회논문집A
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• 제32권10호
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• pp.831-835
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• 2008

We present a continuous electrical cell lysis chip, using a DC bias voltage to generate the focused high electric field for cell lysis as well as the electroosmotic flow for cell transport. The previous cell lysis chips apply an AC voltage between micro-gap electrodes for cell lysis and use pumps or valves for cell transport. The present DC chip generates high electrical field by reducing the width of the channel between a DC electrode pair, while the previous AC chips reducing the gap between an AC electrode pair. The present chip performs continuous cell pumping without using additional flow source, while the previous chips need additional pumps or valves for the discontinuous cell loading and unloading in the lysis chambers. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present chip generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of Red Blood Cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of $30{\mu}m/s{\pm}9{\mu}m/s$. In conclusion, the present chip is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip.