• Journal of Ginseng Research
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• 제30권2호
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• pp.64-69
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• 2006

Gap junctional channels, allowing rapid intercellular communication and synchronization of coupled cell activities, play crucial roles in many signaling processes, including a variety of cell activities. Consequently, a modulation of the gap junctional intercellular communication (GJIC) should be a potential pharmacological target. In the present, the GJIC of a epithelial-derived rat mammary cells (BICR-M1Rk) was assessed in the presence of ginseng saponin, by using an established method of scrape-loading dye transfer assay. The transfer of Lucifer yellow (diameter: 1.2 nm) among the neighboring BICR-M1Rk cells, in which connexin43 (Cx43) is a major gap junction channel-forming protein, was significantly retarded at a concentration of $10{\mu}g/ml$ ginseng saponin. By using both methods of RT-PCR and Western blotting, it was demonstrated that ginseng saponin modulated neither the mRNA synthesis of Cx43 nor the translational process of Cx43. This ginseng saponin-induced modification of GJIC was a similar phenomenon observed under the $\beta$-glycyrrhetinic acid treatment, a well-known gap junction channel blocker. Taken together, it is reasonable to conclude that the ginseng saponin inhibits GJIC only by modulating the gating property of gap junction channels.

• 한국수소및신에너지학회논문집
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• 제33권3호
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• pp.202-208
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• 2022

Pt-Ni nanocatalysts were loaded on carbon black by spontaneous reduction reaction of platinum (II) acetylacetonate and nickel (II) acetylacetonate, and they were characterized by transmission electron microscopy (TEM), thermogravimetric analyzer (TGA), energy dispersive x-ray analyzer (EDS), BET surface area and fuel cell test station. The distribution of the Pt and Ni nanoparticles was observed by TEM, and the loading weight of Pt-Ni nanocatalysts on the carbon black was measured by TGA. The elemental ratio of Pt and Ni was estimated by EDS. It was found that the loading weight of Pt-Ni nanoparticles was 5.54 wt%, and the elemental ratio of Pt and Ni was 0.48:0.35. Specific surface area was measured by BET analysis instrument and I-V characteristics were estimated.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2000년도 제14회 학술강연논문집
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• pp.8-8
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• 2000

In this paper are presented main power and gasdynamic characteristics of C-l6VK hypersonic test cell of Research Test Center of CIAM. Gasdynamic adjustment of the C-l6VK test cell was carried out with the working section constructed on scheme of Ramjet/scramjet test in free stream. Gasdynamic adjustment was conducted stage by stage in tile following sequence. First, check and preparation of all technical systems and checking measuring system. Than determination of the characteristics of test cell on cold (without the heating of air at entrance) regime and determination of the characteristics of test cell on regimes with the heating of air. Finally determination of tile characteristics of test cell with the loading of the working part by object. On tile final stage of gasdynamic adjustment two experiments with tile axisymmetric Scramjet model loaded into the working part of test cell were conducted. The first experiment was conducted with the purpose of determination of flow parameters with the object leaded into the working part and verification of experiment cyclogram. The second experiment was conducted with injection of hydrogen into the combustion chamber of object, that is tile conditions on test cell simulated Scramjet flight Mach number M = 6. Such methodology of gasdynamic adjustment allows to determine influence of experimental object on flow parameters in the working part at different conditions of experiment (with the burning in combustion chamber of object and without the homing), and also to compare flow characteristics in the object duct.

• 대한기계학회논문집A
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• 제27권4호
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• pp.601-607
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• 2003

The column type is used as the sensing element of the load cell to measure the large force in the range of 10$^4$~ 10$^{7}$ N. However. it is not easy to handle the load with large capacity due to its size. It is, therefore, necessary to design a compact load cell with a low aspect ratio. Thus this paper showed the characteristic evaluation of compact load cell with respect to the shape of supporting plate. The supporting plate of the load cell was an annular type with inner(D$_{i}$) and outer(D$_{o}$) diameters. Using the strain distribution obtained from FEM. the supporting plate was designed to get the stable output voltage of the load cell. The three designed supporting plates were manufactured, and the result of characteristic experiment of the load cell, using the 10 MN hydraulic force standard machine, was compared with FEM.M.M.

• 한국전자파학회논문지
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• 제18권6호
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• pp.612-619
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• 2007

본 논문은 Four-wired 안테나를 이용하여 EMI/EMC 실험을 할 수 있는 안테나를 설계, 제작하였다. 일반 TEM-cell과 비교하여 이번 논문에서 설계, 제작하는 안테나의 특징은 무반향실에 broadband balun, Four-wired 안테나와 Loading resistances을 쉽게 설치하여, E-field polarization을 선택 할 수 있다는 점이다. 그리고 공간의 제약없이 기존의 무반향실을 이용하여 적은 비용으로 EMI/EMC 실험 환경을 구축할 수 있다. 제작된 안테나는 S, C 및 X-band에서 EMI/EMC 실험을 할 수 있는 가로, 세로, 높이 모두 약 10 cm 정도의 크기를 갖는 quiet zone을 얻을 수 있었다.

• 한국수소및신에너지학회논문집
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• 제15권1호
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• pp.23-31
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• 2004

For the fabrication of high efficient bifunctional electrocatalyst of oxygen electrode for PEM URFC (Polymer Electrolyte Membrane Unitized Regenerative Fuel Cell), which is a promising energy storage and conversion system using hydrogen as the energy medium, several bifunctional electrocatalysts were prepared and tested in a single cell URFC system. The catalysts for oxygen electrode revealed fuel cell performance in the order of Pt black > PtIr > PtRuOx > PtRu ~ PtRuIr > PtIrOx, whereas water electrolysis performance in the order of PtIr ~ PtIrOx > PtRu > PtRuIr > PtRuOx ~ Pt black. Considering both reaction modes PtIr was the most effective elctrocatalyst for oxygen electrode of present PEM URFC system. In addition, the water electrolysis performance was significantly improved when Ir or IrOx was added to Pt black just 1 wt.% without the decrease of fuel cell performance. Based on the catalyst screening and the optimization of catalyst composition and loading, the optimum catalyst electrodes for PEM URFC were $1.0mg/cm^2$ of Pt black as hydrogen electrode and $2.0mg/cm^2$ of PtIr (99:1) as oxygen electrode.

• KSBB Journal
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• 제8권5호
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• pp.431-437
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• 1993

적은 에너지로 적절한 유동충을 유지하기 위한 가 장 좋은 지지체는 bulk 빛 wet밀도가 가장 작은 활 성탄이며 최소및최적유동화속도가각각0.03cm/sec, 0.25cm/sec로 나타났다. 메탄 발효에 대한 유 기물 부하의 증가에 따라 모든 지지체의 CODe, 제 거율은 감소하였으나, 활성탄은 본 실험범위의 유기 물 부하에서 90% 이상의 제거율을 보였다. 이는 매 우 큰 비표면적을 가지는 활성탄에 많은 미생물이 흡착된 때문으로 사료된다. 유기물 부하 $16gCOD_{cr}/\ell.day$16gCODcr/day에서 흡착된 마생물의 양은 157mg/g이다. 천연 제올라이트와 Roast Celite를 비교하면 바표 면적만에 비례하여 미생물의 양이 증가하지는 않음 을 알 수 았으며, 동엘한 비표면적을 가지는 Roast Celite와 부석을 비교하면, 유기물 제거능이 Roast Celite가 우수한데 이로부터 표면의 거칠기가 상대적으로 크고 표면전하가 양전하를 띠는 것이 마생물 흡착에 중요한 것으로 사료된다. 혐기성 유동층 반 응기에 이용할 지지체는 유체의 shear stress를 감소 시켜서 미생물 흡착을 증대시키기 위하여 될 수 있으면 작은 wet밀도와 작은 유동화 속도를 가져야 한다고 사료된다.

• 한국해양바이오학회지
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• 제14권2호
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• pp.112-123
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• 2022

Photosynthetic bacteria (PSB) play an important role in water purification, and their application is beneficial for sustainable aquaculture. However, maintaining the microbial stability of PSB from subculturing to preservation is a challenging task. Since improvement in the microbial stability of PSB is a crucial parameter, optimized conditions for cell immobilization and lyophilization were investigated. In PSB immobilization, 0.1-M CaCl₂ was found to be the most effective divalent metal ion solution in terms of cost-effectiveness, resulting in beads with a 4-mm diameter and high loading (1.91×10⁹ CFU/mL) of viable cells. Maintenance of cell viability, external appearance, and color of PSB beads was best in 3.5% NaCl during storage. In lyophilization, the addition of skim milk (9%) and dextrose (2%) as cryoprotective additives allowed the highest cell viability. Over an 18-week shrimp breeding period, when optimally manufactured beads and lyophilized powder of PSB were applied to shrimp aquaculture water, NH⁴⁺, NO₃^-, and NO₂^- were more effectively removed by 55%, 100%, and 100%, respectively, compared to controls. Thus, microbial stability of PSB through optimized cell immobilization and lyophilization was successfully enhanced, enabling a wide application.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.360.1-360.1
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• 2016

Hydroxyapatite (Ca10(PO4)6(OH)2, HAP) particles have attracted a great deal of attention in biomedical fields due to their good biocompatibility, bioactivity and fairly broad applications as drug delivery, dental implant, bone cement, and etc. Thus, many researchers have made an effort to add new functionalities such as luminescence, drug delivery, and bone regeneration properties up to HAP powders by controlling their nanostructure as well as composition. In this research, the mesoporous strontium substituted HAP (Sr-HAP) microspheres were synthesized using a hydrothermal method. In this synthesis, aspartic acid monomers were utilized to form microsphere by controlling surface energy of HAP particles and Sr ions were substituted into Ca ion sites, which induced luminescence property in HAP powders. Moreover, the change in the amount of Sr substitution was found to influence the particle size, morphology, and concurrently surface area, which led to changing drug loading as well as drug release property. The amount of Sr influences the morphology, luminescent properties, particle size, surface area cell viability and drug loading property, which are investigated by SEM, TEM, XRD, FTIR, BET, XPS and in vitro test such as MTT assay and drug release test. In particular, the multifunctional Sr-HAP with molar ratios of 0.25 (Sr/(Ca+Sr)) possessed the strongest luminescent property as well as the superior drug loading and sustained release properties that were correspondent with large surface area and pore size. Our study indicates that the fabricated multifunctional Sr-HAP microspheres are quite useful for bone regeneration and drug delivery.

• 한국자동차공학회논문집
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• 제9권5호
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• pp.195-205
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• 2001

The mechanical properties of aluminum honeycomb on the direction of axial crushing under quasistatic loading test was investigated. The crushing process was simulated numerically by full-scale finite element models. Simulations reproduce the experimental results both qualitatively as well as quantitatively. From the investigation, we suggested the constitutive model of energy absorbing honeycomb structure for large scale impact analysis. Real impact test of the WB(Moving Deformable Barrier) was carried and compared with finite element simulation. Constitutive model used in the numerical simulation had a good correlation with experiment. By suggesting the optimizing method fur honeycomb cell configuration design, relationship between cell configuration and crush strength is studied.