• Title/Summary/Keyword: Cell Area

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Morphological Measurement of the Spermatogenic Cells in the Korean Native Pheasant(Phasianus colchicus korpowi) with Image Analyser (영상분석기를 이용한 한국산 꿩의 정자발생세포들의 형태학적 계측)

  • 박영석;양홍현;김인식
    • Korean Journal of Animal Reproduction
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    • v.23 no.1
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    • pp.37-43
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    • 1999
  • The image analyser was used for the measurement of the morphological changes of the spermatogenic cells and seminiferous tubules in the pheasant during the breeding and non breeding season. 1. The seminiferous tubules were enlarged 2.57 times during the breeding season than during the non breeding season. Only Sertoli cells and spermatogonia in the seminiferous tubules can be found during the non breeding season. 2. There is no significantly difference in the ratio of nucleus area against that of cell size in the spermatogonium between breeding and non breeding season. The ratio of area was 28.71% and 29.11%, respectively. However, the enlargement of spermatogonium was noticed during the non breeding season. 3. The highest value of the ratio of the nucleus area against that of cell size among the germ cells was measured 37.40% in the pachytene phase of the spermatocyte during the breeding season. 4. The ratio of nucleus area against that of cell size in the spermatid was 22.53%.

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Morphological Study of Microfilariae Found in the Area of Yongju-Gun (영주지방에 분포하는 사상충 자충에 대한 형태학적 연구)

  • 김훈수
    • The Korean Journal of Zoology
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    • v.8 no.1
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    • pp.11-14
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    • 1965
  • The object to this study is to identify the genus and species of the microfilariae which were recently found in the area of Youngju-Gun. THe identification of the microfilariae was made on the morphologicla aspects. 1. Blood samples were collected through vena punction from known microfilariae carriers living in the newly confirmed filaria endemic area of Youngju0Gun in Jyongsang Pukdo Province. Youngju-Gun is located in the mountainous central part of Korean Peninsula. 2. The following fixation and staining techniques were applied. (1) Fixation by drying in the air, followed by staining with Azeo or Giemsa. (2) Knott's fixation method (2% formalin), followed by staining with Azur II. 3. A comparative study of the body length of the microfilariae after different fixation and staining techniques were applied. (1) Knott's fixation method followed by staining with Azur II : average body length found was 28.4$\mu$. (2) Dry fixation followed by staining with Giemsa : average body length found was 209.4$\mu$. (3) Dry fixation followed by staining with Azeo : average body length found was 205.4$\mu$. 4. The locations of the different body cells were measured in 60 individuals of microfilariae in the wet preparation fixed by Knott's method and stained with Azur II. The distance of the different body cells to cephalic apex of microfilariae was measured and calculated as a percentage of the total body length. The average results are as follows : BNC , 3.04% ; N, 22.74%, EP, 31.4% ; EC, 37.77%, G1 cell , 67.94%; G2 cell, 73.54% : G3 cell , 75.55% ; G4 cell, 77.65% ; AP, 82.02%%. 5. As a result of the above findings the microfilariae found in the above mentioned area could be identified as Brugia malai(BRUG, 1927) BUCKLEY, 1960.

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A Low-power DIF Radix-4 FFT Processor for OFDM Systems Using CORDIC Algorithm (CORDIC을 이용한 OFDM용 저전력 DIF Radix-4 FFT 프로세서)

  • Jang, Young-Beom;Choi, Dong-Kyu;Kim, Do-Han
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.45 no.3
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    • pp.103-110
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    • 2008
  • In this paper, an efficient butterfly structure for 8K/2K-Point Radix-4 FFT algorithm using CORDIC(coordinate rotation digital computer) is proposed. It is shown that CORDIC can be efficiently used in twiddle factor calculation of the Radix-4 FFT algorithm. The Verilog-HDL coding results for the proposed CORDIC butterfly structure show 36.9% cell area reduction comparison with those of the conventional multiplier butterfly structure. Furthermore, the 8K/2K-point Radix-4 pipeline structure using the proposed butterfly and delay commutators is compared with other conventional structures. Implementation coding results show 11.6% cell area reduction. Due to its efficient processing scheme, the proposed FFT structure can be widely used in large size of FFT like OFDM Modem.

Thermal and Flow Analysis in a Proton Exchange Membrane Fuel Cell

  • Jung, Hye-Mi;Koo, Ja-Ye
    • Journal of Mechanical Science and Technology
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    • v.17 no.9
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    • pp.1358-1370
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    • 2003
  • The effects of anode, cathode, and cooling channels for a Proton Exchange Membrane Fuel Cell (PEMFC) on flow fields have been investigated numerically. Continuous open-faced fluid flow channels formed in the surface of the bipolar plates traverse the central area of the plate surface in a plurality of passes such as a serpentine manner. The pressure distributions and velocity profiles of the hydrogen, air and water channels on bipolar plates of the PEMFC are analyzed using a two-dimensional simulation. The conservation equations of mass, momentum, and energy in the three-dimensional flow solver are modified to include electro-chemical characteristics of the fuel cell. In our three-dimensional numerical simulations, the operation of electro-chemical in Membrane Electrolyte Assembly (MEA) is assumed to be steady-state, involving multi-species. Supplied gases are consumed by chemical reaction. The distributions of oxygen and hydrogen concentration with constant humidity are calculated. The concentration of hydrogen is the highest at the center region of the active area, while the concentration of oxygen is the highest at the inlet region. The flow and thermal profiles are evaluated to determine the flow patterns of gas supplied and cooling plates for an optimal fuel cell stack design.

Electrode fabrication and Performance of Single Cell with an area of $2000\;cm^2$ in PAFC ($2000\;cm^2$ 대면적 인산형 연료전지 전극 제조 및 단위전지 성능 특성)

  • Song, Rak-Hyun;Shin, Dong-Ryul;Kim, Chang-Soo;Lee, Byung-Rok;Choi, Soo-Hyun
    • Proceedings of the KIEE Conference
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    • 1997.07d
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    • pp.1308-1310
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    • 1997
  • Several methods for electrode fabrication of phosphoric acid fuel cell(PAFC) have been studied. The conventional methods that include a coating method and a rolling method, have disadvantages of a very complicated drying process and a hot pressing process for making a large electrode. In this study, to solve these problems, the mixing method of coating and rolling processes has been developed. In the mixing method, the electrode was coated on the electrode support and, after drying the coated layer, was rolled at room temperature and then sintered at $350^{\circ}C$. The single cell performances of the electrodes fabricated by several methods were examined and the mixing method appeared a good cell performance of 0.65 V, $260\;mA/cm^2$. Also the single cell with an area of $2000\;cm^2$ was manufactured and its performance attained 0.593 V, 300 A.

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Novel Maritime Wireless Communication based on Mobile Technology for the Safety of Navigation: LTE-Maritime focusing on the Cell Planning and its Verification

  • Shim, Woo-Seong;Kim, Bu-Young;Park, Chan-Yong;Lee, Byeong-Hyeok
    • Journal of Navigation and Port Research
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    • v.45 no.5
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    • pp.231-237
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    • 2021
  • Enhancing the performance of maritime wireless communication has been highlighted by the issue of cell planning in the sea area because of lack of an appropriate Propagation Loss Model (PLM). To resolve the cell planning issue in vast sea areas, it was essential to develop the (PLM) matching the intended sea area. However, there were considerable gaps between the prediction of legacy PLMs and field measurement in propagation loss and there was a need to develop the adjusted PLM (A-PLM). Therefore, cell planning was performed on this adjusted model, including modification of the base station's location, altitude, and antenna azimuth to meet the quality objectives. Furthermore, in order to verify the availability of the cell planning, Communication Service Quality Monitoring System (CS-QMS) was developed in the LTE-Maritime project to collect LTE signal quality information from the onboard equipment at regular intervals and to ensure that the service quality was high enough to satisfy the goals in each designated grid. As a result of verification, the success rate of RSRP was 95.7% for the intensive management zone (IMZ) and 96.4% for the interested zone (IZ), respectively.

PEMFC Characterization Study by in-situ Analysis Method (In-Situ 분석법에 의한 연료전지 특성 연구)

  • Kim, Young-Min;Lee, Jong-Hyun;Im, Se-Joon;Ahn, Byung-Ki;Lim, Tae-Won
    • Transactions of the Korean hydrogen and new energy society
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    • v.20 no.3
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    • pp.208-215
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    • 2009
  • PEMFC stack power output is needed to be around 100 kW to meet the requirements of automotive application and scaling-up the active area of the stack cells will allow a higher power. In the case of scaling-up the active area of cells, it is difficult to obtain uniform in-plane internal conditions such as temperature, relative humidity and stoichiometry of the feed gas. These ununiformity with the location in the cell would affect both the performance and durability of the stack, so it is important to understand phenomena in the cell for improving them. In this study, the current density, electrochemical resistance and performance distribution measurement was performed to understand the ununiformity in a single cell using in-situ method; (1) Current Density Distribution (CDD) Device and (2) Segmented Cell Fixture. The influence of location of feed gas on the performance of a single cell was experimentally measured and discussed by using a segmented single cell which was composed of 8 compartments. The correlation between the location and performance in a single cell was discussed by these two tools and it was extended between the local characterization and the durability in a MEA by comparing the used cell with a fresh one. It was also studied in terms of electrochemistry by Electrochemical Impedance Spectroscopy.

A Study of Temperature Distribution and Flooding Phenomena of Cathode now Channel in a PEM Unit Fuel Cell (고분자전해질형 단위 연료전지의 공기극 유로 채널 내 온도 분포와 플러딩 현상에 관한 연구)

  • Kim, Han-Sang;Ha, Tae-Hun;Min, Kyoung-Doug
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.11a
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    • pp.101-104
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    • 2006
  • Water management is considered to be one of the main issues to be addressed for the performance improvement of proton exchange membrane (PEM) fuel cell. For good water management, the detailed information on the water distribution inside an operating PEM fuel cell should be available to main an adequate level of hydration in the PEM While avoiding performance decline due to liquid rater flooding. For the PEM fuel cell to be commercially viable as vehicle applications, the flooding on the cathode side should be minimized during the fuel ceil operation. In this study to investigate cathode flooding and its relation with temperature distribution in flow channels, visualization study was performed on the cathode side of a PEM fuel cell. For the direct visualization of temperature field and water transport in cathode flow channels, a transparent cell was designed and manufactured using quartz window. Water transport and its two-phase flow characteristics in flow channels were investigated experimentally. Also, the visualization of temperature distribution In cathode flow channels was made by using IR camera. Results indicated that the temperature rise near the exit of cathode flow channel was found. It is found that this area corresponds to the flooding area from both temperature and flooding visualization results It is expected that this study can effectively contribute to get the detailed data on water transport linked with heat management during the operation of a PEM fuel cell

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ELECTRO-MICROSCOPE BASED 3D PLANT CELL IMAGE PROCESSING METHOD

  • Lee, Choong-Ho;Umeda Mikio;Takesi Sugimoto
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11b
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    • pp.227-235
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    • 2000
  • Agricultural products are easily deformable its shape because of some external forces. However, these force behavior is difficult to measure quantitatively. Until now, many researches on the mechanical property was performed with various methods such as material testing, chemical analysis and non-destructive methods. In order to investigate force behavior on the cellular unit of agricultural products, electro-microscope based 3D image processing method will contribute to analysis of plant cells behavior. Before image measurement of plant cells, plant sample was cut off cross-sectioned area in a size of almost 300-400 ${\mu}$ m units using the micron thickness device, and some of preprocessing procedure was performed with fixing and dyeing. However, the wall structure of plant cell is closely neighbor each other, it is necessary to separate its boundary pixel. Therefore, image merging and shrinking algorithm was adopted to avoid disconnection. After then, boundary pixel was traced through thinning algorithm. Each image from the electro-microscope has a information of x,y position and its height along the z axis cross sectioned image plane. 3D image was constructed using the continuous image combination. Major feature was acquired from a fault image and measured area, thickness of cell wall, shape and unit cell volume. The shape of plant cell was consist of multiple facet shape. Through this measured information, it is possible to construct for structure shape of unit plant cell. This micro unit image processing techniques will contribute to the filed of agricultural mechanical property and will use to construct unit cell model of each agricultural products and information of boundary will use for finite element analysis on unit cell image.

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Cell Image Processing Methods for Automatic Cell Pattern Recognition and Morphological Analysis of Mesenchymal Stem Cells - An Algorithm for Cell Classification and Adaptive Brightness Correction -

  • Lim, Kitaek;Park, Soo Hyun;Kim, Jangho;SeonWoo, Hoon;Choung, Pill-Hoon;Chung, Jong Hoon
    • Journal of Biosystems Engineering
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    • v.38 no.1
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    • pp.55-63
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    • 2013
  • Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.