• 한국식품과학회지
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• 제28권2호
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• pp.352-359
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• 1996

새로운 천연식용색소원으로 기대되는 자색고구마 anthocyanin 색소의 안정성에 관한 기초연구로서 자색고구마 색소에 대한 pH, 당, 유기산, 금속이온, ascorbic acid및 광 등의 영향을 조사하였다 자색고구마 색소는 pH가 낮을수록 색깔의 강도가 높고 안정한 경향을 나타냈으며 , pH가 증가함에 따라 최대흡수파장이 장파장쪽으로 이동하는 bathochromic shift 현상을 나타냈다. 첨가한 당류 중 glucose는 색소의 안정성에 기여하였으며, sucrose와 fructose는 역효과를 나타냈다. 유기산의 첨가는 색소의 안정화에 크게 기여하였으며, ctric acid가 가장 효과적이었으며, 다음으로 malic acid, tartaric acid, succinic acid 순으로 나타났다. 금속이온은 색소의 안정성을 떨어뜨렸으나, $Al^{3+}$과 $Cd^{2+}은 자색고구마 anthocyanin색소의 안정성에 기여하는 것으로 나타났다. Ascorbic acid는 색소의 안정성을 저해하였으며 산소 존재하에서는 그 정도가 컸다. 자색고구마 색소의 안정성은 광에 의해 크게 저하되었는데, 형광보다 일광에 대한 영향이 더 켰으며, 저장시 광을 차단함으로써 안정성을 증진시킬 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제5권2호
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• pp.68-73
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• 1995

Pseudomonas sp. P20 was shown to be capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce the corresponding benzoic acids wnich were not further degraded. But the potential of the strain for biodegradation of 4CB was shown to be excellent. The pcbA, B, C and D genes responsible for the aromatic ring-cleavage of biphenyl and 4CB degradation were cloned from the chromosomal DNA of the strain. In this study, the pebC and D genes specifying degradation of 2, 3-dihydroxybiphenyl (2, 3-DHBP) produced from biphenyl by the pebAB-encoded enzymes were cloned by using pBluescript SK(＋) as a vector. From the pCK102 (9.3 kb) containing pebC and D genes, pCK1022 inserted with a EcoRI-HindIII DNA fragment (4.1 kb) carrying pebC and D and a pCK1092 inserted with EcoRI-XbaI fragment (1.95 kb) carrying pebC were constructed. The expression of pcbC and D' in E. coli CK102 and pebC in E. coli CK1092 was examined by gas chromatography and UV-vis spectrophotometry. 2.3-dihydroxybiphenyl was readily degraded to produce meta-cleavage product (MCP) by E. coli CK102 after incubation for 10 min, and then only benzoic acid(BA) was detected in the 24-h old culture. The MCP was detected in E. coli CK1022 containing pebC and 0 genes (by the resting cells assay) for up to 3 h after incubation and then diminished completely in 8 h, whereas the MCP accumulated in the E. coli CK1092 culture even after 6 h of incubation. The 2, 3-DHBP dioxygenases (product of pebC gene) produced by E. coli CK1, CK102, CK1023, and CK1092 strains were measured by native PAGE analysis to be about 250 kDa in molecular weight, which were about same as those of Pseudomonas sp. DJ-12, P. pseudoa1caligenes KF707, and P. putida OU83.

• Archives of Pharmacal Research
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• 제29권12호
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• pp.1147-1153
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• 2006

Mizoribine (MZR) has been shown to possess immunosuppressive activity that selectively inhibits the proliferation of lymphocytes by interfering with inosine monophosphate dehydrogenase. The efficacy of MZR is not only in patients who have had renal transplantation, but also in patients with rheumatoid arthritis (RA), lupus nephritis, and primary nephritic syndrome. Because the exact mechanism of its immunosuppressive action is not clear, the object of this study was to examine the ability of MZR to regulate the antigen presenting cells (APCs), dendritic cells (DCs). In this work, we tested whether MZR ($1{\sim}10\;{\mu}g/mL$) could inhibit the cross-presentation of DCs. DC2.4 cells ($H-2K^{b}$) or bone marrow-derived DCs (BM-DCs) generated from BM cells of C57BL/6 mouse ($H-2K^{b}$) were cultured in the presence of MZR with OVA-microspheres, and the amount of OVA peptide-class I MHC complexes was measured by a T cell hybridoma, B3Z, that recognizes OVA (257-264 : SIINFEKL)-$H-2K^{b}$ complex and expresses-galactosidase. MZR profoundly inhibited the expression of SIINFEKL-$H-2K^{b}$ complexes. This inhibitory activity of MZR appeared to affect the phagocytic activity of DCs. MZR also decreased IL-2 production when we examined the effects of MZR on $CD4^{+}$ T cells. These results provide an understanding of the mechanism of immunosuppressive activity of MZR on the inhibition of MHC-restricted antigen presentation and phagocytic activity in relation to their actions on APCs.

• 한국전기전자재료학회논문지
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• 제17권11호
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• pp.1212-1217
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• 2004

Red organic electroluminescent (EL) devices based on tris(8-hydroxyquinorine aluminum) (Alq$_3$) doped with red emissive materials, 4-(dicyanomethylene)-2-t-butyl -6-(l,1,7,7-tetramethyljulolidyl-9-enyl)4H-pyran (DCJTB). poly(3-hexylthiophene) (P3HT). rubrene and 4-dicyanomethylene-2-methyl-6[2-(2,3.6.7-tetrahydro-lH,5H-benzo-[i,j]quinolizin-8yl)vinyl]-4H-pyran (DCM2) were fabricated for applying to the red light source, The photoluminescence (pL) intensities of red emissive materials doped in Alq$_3$ are limited by the concentration quenching with increasing the doping ratio and the doping concentration of DCJTB, DCM2, P3HT and rubrene measured at the maximum intensity showed 5, 1, 0.5 and 2 wt%, respectively. Time-resolved PL dynamic results showed that the PL lifetime of red emissive materials doped in Alq$_3$ were increased more than the value of material itself. It means that the efficient energy transfer occurred in the mixed state and Alq$_3$ is a suitable host materials for red emissive materials, The device which was used DCJTB as a dopant achieved the best result of the maximum luminance of 594 cd/$m^2$ at 15 V and showed the chromaticity coordinates of x=0,624, y=0,371.

• 대한치과교정학회지
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• 제31권1호
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• pp.39-50
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• 2001

교정치료를 함에 있어 하악골의 모양, 성장률, 성장방향에 대한 예측은 치료계획의 수립 및 치료기간과 예후를 결정하는데 있어 중요한 역할을 한다. 이를 규명하기 위해 다양한 방법들이 시도 되어왔고 측모두부방사선규격사진을 이용함으로써 좀 더 정확성을 기할 수 있게 되었다. 그러나 해부학적인 구조의 특성상 하악과두 부위에서 중첩이 발생하여 정확한 형태 및 위치의 측정에 의문이 남는다. 이 연구의 목적은 하악과두 부위에서 중첩을 피하여 좀 더 정확한 하악골의 형태 및 성장양상을 알아보고자 함에 있다. 이 연구는 1급 부정교합군 14명, 2급 부정교합군 9명, 3급 부정교합군 17명, 총 40명의 환자를 대상으로 평균 4년 3개월의 기간을 두고 최대개구상태의 측모두부방사선규격사진(Wide Open lateral cephalogram)을 촬영하여 11개의 계측항목을 설정, 계측하였고 Autocad Rl4 program을 이용하여 평균적인 외형을 작도하고 측면적을 계측하여 비교, 분석하였다. 그 결과 다음의 결론을 얻었다. 1 하악체의 길이(gonion-menton)는 3급, 1급, 2급 순으로 증가추세를 보였고 3급 부정교합군이 2급 부정교합군에 비해 2배 이상 증가하였으나 통계학적으로는 3급 부정교합군과 1급 부정교합군, 2급 부정교합군과 1급 부정교합군 사이에는 유의성이 있었고 3급 부정교합군과 2급 부정교합군 사이에는 유의한 상관성이 없는 것으로 나타났다(p<0.05). 2. Go-Me, A-Cd, B-Cp, E-F, G-H, 측면적의 항목에서 연 평균 성장량, 성장속도 들에서 3급 부정교합군에서 많은 증가를 보이고 특히 2급 부정교합군에 비해서는 명확한 차이를 보이고 있다. 3. 평균 4년 3개월 동안의 하악골 측면적의 연 평균 성장량은 1급 부정교합군이 $1.0cm^2$, 2급 부정교합군이 $0.8cm^2$, 3급 부정교합군이 $1.4cm^2$이고 성장율은 $11.9\%,\;11.8\%,\;20.3\%$로 1급 부정 교합군과 2급 부정교합군에서는 별 차이가 보이지 않았고 3급 부정교합군에서는 다른 두 군에 비해 2배에 가까운 성장율을 보였다. 이상의 결과에서 부정교합의 유형에 따른 하악골의 성장량의 차는 선천적인 악골의 크기에 의해서 라기 보다는 성장 중 발생하는 하악골의 성장속도와 성장량의 차이에 의한 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1691-1695
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• 2005

The bovine lymphocyte antigen (BoLA)-DRB3 gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. Since different alleles favour the binding of different peptides, DRB3 has been extensively evaluated as a candidate marker for associations with various bovine diseases and immunological traits. For that reason, the genetic diversity of the bovine class II DRB3 locus was investigated by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). This study describes genetic variability in the BoLA-DRB3 in Iranian Golpayegani Cattle. Iranian Golpayegani Cows (n = 50) were genotyped for bovine lymphocyte antigen (BoLA)-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism method. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, HaeIII and BstYI was conducted on the DNA from Iranian Golpayegani Cattle. In the Iranian Golpayegani herd studied, we identified 19 alleles.DRB3.2${\times}$16 had the highest allelic frequency (14%), followed by DRB3.2${\times}$7 (11%). Six alleles (DRB3.2${\times}$25, ${\times}$24, ${\times}$22, ${\times}$20, ${\times}$15, ${\times}$3) had frequencies = 2%. Although additional studies are required to confirm the present findings, our results indicate that exon 2 of the BoLA-DRB3 gene is highly polymorphic in Iranian Golpayegani Cattle.

• 물과 미래
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• 제12권2호
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• pp.36-42
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• 1979

This study has been conducted to find out the location and amount of the subterrain water body developed in the alluvial stratum in Paho-Dong, Sungsee-Myo-n, Dalsung-Kun are. An earlier test drilling was done in this area by R.O.K. Agricultural Promotion Corporation. The area consists of a small river basin and surrounding low hills developed around the junction of the Nakdong and Kumho Rivers. The strata of this area are made of Paldal gravel, Bokhyundong and Banyawol layers which were formed in the cretaceous period of the Meso-saicera or acid dikes and covered with-irregular alluvial layers. The alluvial layer in this area is composed of rather minute particles and proportional electric resistance tests on this layer show $10^2\;-\;10^3\;\Omega/cm$. The drillings up to 12meters deep showed only the sand layer (Form 3 to 26meters in thickness) contains water. The sand layers can not be considered a good water trapping one. Applying the data from the drillings to A.Hazen's equation, $K\;=\;{cd_e}^2\;(0.7\;+0.03t)$ to get the theoretical value of the water infilterated, I calculated it as K=13.92m/day. And again the value was set to Dupuit equation, (equation omitted) to acquire the pumping water amount the result was $Q_1\;=\;77.20\;\textrm{m}^3/day$. When the data-applied to the equation for pumping water amount, (equation omitted), the results were $Q_2\;=\;122.39\;\textrm{m}^3/day$ and K = 38m/day $Q_1\;and;Q_2$ (tow types of pumping water amount) represent proper value decrease and maximum value decrease respectively. Therefore, $Q_2$ is the least amount of water we can pump. The area covers about $1,555,000\;\textrm{m}^2$ and the maximum water needed in this area amounts to $155,000\textrm{m}^3$. That means we have to drill 1,406 pumping wells. It is concluded that undertaking the project in this area is irrational or even desperate and surface water should be developed more favorably.

• The Korean Journal of Physiology
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• 제20권1호
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• pp.43-52
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• 1986

The influences of extracellular $Ca^{2+}\;and\;K^+$ upon the spike action potentials were studied in isolated uterine strips of rat. Regular, rhythmic uterine contractions were induced by the administration of oxytocin$(0.2{\sim}0.5\;I.U.)$, and recorded with force transducer. Spike action potentials were extracellularly measured by use of suction electrode, and compared with those recorded intracellularly by glass microelectrode. The results obtained were as follows : 1) The frequency and duration of spike bursts, and the number of spikes in a burst could be analyzed by use of both methods. But the absolute values of membrane potential were not measurable with the suction electrode. 2) The duration of contraction$(CD_{90};\;the\;duration\;of\;90%\;relaxation)$ was lengthened from the control 17.0 sec to 20.6 sec, in parallel with the increase of spike number from the control 21 to 26, as the increase in $Ca^{2+}$ concentration from 2 to 4 mM. 3) The amplitude and frequency of contractions were gradually decreased, simultaneously with the decrease in the number of spikes in a burst, when the $Ca^{2+}-antagonist$, verapamil was administered cumulatively. 4) The number of spikes was changed from the control 15 to 7, in cabs of the administration of ver)'low dose of verapamil$(10^{-6}\;g/l)$. 5) Increase in the numbers of spike bursts was well matched to the increase in frequency of contractions when extracellular $K^+$ was increased.

• BMB Reports
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• 제55권2호
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• pp.81-86
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• 2022

Macrophages are a major cellular component of innate immunity and are mainly known to have phagocytic activity. In the tumor microenvironment (TME), they can be differentiated into tumor-associated macrophages (TAMs). As the most abundant immune cells in the TME, TAMs promote tumor progression by enhancing angiogenesis, suppressing T cells and increasing immunosuppressive cytokine production. N-myc downstream-regulated gene 2 (NDRG2) is a tumor suppressor gene, whose expression is down-regulated in various cancers. However, the effect of NDRG2 on the differentiation of macrophages into TAMs in breast cancer remains elusive. In this study, we investigated the effect of NDRG2 expression in breast cancer cells on the differentiation of macrophages into TAMs. Compared to tumor cell-conditioned medium (TCCM) from 4T1-mock cells, TCCM from NDRG2-over-expressing 4T1 mouse breast cancer cells did not significantly change the morphology of RAW 264.7 cells. However, TCCM from 4T1-NDRG2 cells reduced the mRNA levels of TAM-related genes, including MR1, IL-10, ARG1 and iNOS, in RAW 264.7 cells. In addition, TCCM from 4T1-NDRG2 cells reduced the expression of TAM-related surface markers, such as CD206, in peritoneal macrophages (PEM). The mRNA expression of TAM-related genes, including IL-10, YM1, FIZZ1, MR1, ARG1 and iNOS, was also downregulated by TCCM from 4T1-NDRG2 cells. Remarkably, TCCM from 4T1-NDRG2 cells reduced the expression of PD-L1 and Fra-1 as well as the production of GM-CSF, IL-10 and ROS, leading to the attenuation of T cell-inhibitory activity of PEM. These data showed that compared with TCCM from 4T1-mock cells, TCCM from 4T1-NDRG2 cells suppressed the TAM differentiation and activation. Collectively, these results suggest that NDRG2 expression in breast cancer may reduce the differentiation of macrophages into TAMs in the TME.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 춘계학술대회 논문집 유기절연재료 전자세라믹 방전플라즈마 일렉트렛트 및 응용기술
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• pp.108-111
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• 2002

CIGS film has been fabricated on soda-lime glass, which is coated with Mo film. by multi-source evaporation process. The films has been prepared with thickness of 1.0 ${\mu}m$, 1.75${\mu}m$, 2.0${\mu}m$, 2.3${\mu}m$, and 3.0${\mu}m$. X-ray diffraction analysis with film thickness shows that CIGS films exhibit a strong (112) preferred orientation. Furthermore. CIGS films exhibited distinctly decreasing the full width of half-maximum and (112) preferred peak with film thickness. Also, The film's microstructure, such as the preferred orientation, the full width at half-maximum(FWHM), and the interplanar spacing were examined by X-ray diffraction. The preparation condition and the characteristics of the unit layers were as followings ; Mo back contact DC sputter, CIGS absorber layer : three-stage coevaporation, CdS buffer layer : chemical bath deposition, ZnO window layer : RF sputtering, $MgF_2$ antireflectance : E-gun evaporation