• Journal of Microbiology and Biotechnology
• /
• 제29권3호
• /
• pp.489-499
• /
• 2019

Subunit vaccines are safer and more stable than live vaccines although they have the disadvantage of eliciting poor immune response. To develop a subunit vaccine, an effective delivery system targeting the key elements of the protective immune response is a prerequisite. In this study, oxidized carbon nanospheres (OCNs) were used as a subunit vaccine delivery system and tuberculosis (TB) was chosen as a model disease. TB is among the deadliest infectious diseases worldwide and an effective vaccine is urgently needed. The ability of OCNs to deliver recombinant Mycobacterium tuberculosis (Mtb) proteins, Ag85B and HspX, into bone marrow derived macrophages (BMDMs) and dendritic cells (BMDCs) was investigated. For immunization, OCNs were mixed with the two TB antigens as well as the adjuvant monophosphoryl lipid A (MPL). The protective efficacy was analyzed in vaccinated mice by aerosol Mtb challenge with a virulent strain of Mtb and the bacterial burdens were measured. The results showed that OCNs are highly effective in delivering Mtb proteins into the cytosol of BMDMs and BMDCs. Upon immunization, this vaccine formula induced robust Th1 immune response characterized by cytokine profiles from restimulated splenocytes and specific antibody titer. More importantly, enhanced cytotoxic $CD8^+$ T cell activation was observed. However, it did not reduce the bacteria burden in the lung and spleen from the aerosol Mtb challenge. Taken together, OCNs are highly effective in delivering subunit protein vaccine and induce robust Th1 and $CD8^+$ T cell response. This vaccine delivery system is suitable for application in settings where cell-mediated immune response is needed.

• 한방안이비인후피부과학회지
• /
• 제14권1호
• /
• pp.129-153
• /
• 2001

Background: SMG (升麻葛根湯加味方) is an herbal medicine which has been used in oriental medicine as a traditional therapeutic agent of pruritus and skin disease. Objective: This study was performed to investigate the effect of SMG on the anti-hypersensitivity and immune response in the murine of type I hypersensitivity induced by the experiment. Materials and Methods: Laboratory rats were primary sensitized with OA (ovalbumin); on day 1, rats of a Control group and Sample group (SMG group) were systemically immunized by subcutaneous injection of 1mg OA and 300mg of Al(OH)3 in a total volume of 2ml saline. The rats of the sample group were orally administered with an SMG water extract for 14 days after primary immunization. On day 14 after the systemic immunization, rats received local immunization by inhaling $0.9\%$ saline aerosol containing $2\%$(wt/vol) OA. A day after local immunization, BAL fluid and peripheral blood were collected from the rats. Total cell, lymphocyte, $CD4^+\;T\;cell,\;CD8^+\;T\;cell,\;CD4^+/CD8^+$ ratio in the BALF, and IgE, $CD4^+\;T\;cell,\;CD8^+$ T cell in the peripheral blood were measured and evaluated. Results: SMG showed a suppressive effect on the immune response in the rats. 1. Total Cells in the BALF decreased in the SMG treated group in comparison group, but statistic differences were not observed. 2. Total lymphocytes in the BALF were statistically decreased in SMG treated group in comparison to the control group. 3. CD4+ T cells in the BALF were statistically decreased in SMG treated group in comparison to the control group. 4. CD8+ T cells in the BALF were decreased in SMG treated group in comparison to the control group, but statistic differences were not observed. 5. The ratio of CD4+/CD8+ in the BALF was statistically decreased in SMG treated group in comparison to the control group. 6. The IgE level in serum was statistically decreased in SMG treated group in comparison to the control group. 7. The ratio of CD4+ and CD8+ in peripheral blood showed undetectable differences between each group of rats. From the experiment cited above, this study shows that SMG has both anti-hypersensitivity effects and immunoregulatory effects when administered to rats. Based on this experiment, it is suggested that SMG could be a useful immunomodulator and anti-allergy agent.

• 한국대기환경학회지
• /
• 제12권1호
• /
• pp.101-112
• /
• 1996

Dry and wet deposition is an impertant removal mechanism of the amobient aerosol in the atmospheric environment. Since the deposition flut provides adverse impacts on various encironmental media including aquatic and ecological system as well as human health, it is essential to quantitatively estimate the removal fluxes of many air pollutants. Thus, the purposes of this experimental study are to investigate seasonal deposition flux variations of the total dustfall and various inorganic elements in the local ambient air and then to finally estimate their dry deposition velocities. To perform the study, the total of 90 dustfall samples were collected from January, 1994 thru February, 1995 in 5 different cities of Korea including Seoul, Suwon, Daejon, Kwangju, and Kangrung. Each sample was analyzed by an AAS and an ICP to determine the quantities of the 11 inorganic elements, such as Zn, Cd, Cr, K, Na, Pb, Ca, Fe, Mn, Mi, and Cu. As results, deposition fluxes, soluble/insoluble fractions, and deposition velocities for each element were extensively investigated. The resulting dry deposition velocities of some elements in Suwon were estimated by ranges of 0.57 .sim. 0.87 cm/sec for Zn, 0.35 .sim. 0.45 cm/sec for Pb, 1.25 .sim. 3.52 cm/sec for Ca, 0.21 .sim. 0.48 cm/sec for Fe, 0.95 .sim. 9.31 cm/sec for Mn, and 2.08 cm/sec for Cu.

• 한국입자에어로졸학회지
• /
• 제8권2호
• /
• pp.47-54
• /
• 2012

Paved road dust emissions were investigated 14 times on 12 main roads in Seo-Cho Gu, Seoul, Korea by vehicle-based mobile sampling system(Road Dust Monitoring System) during September to December 2011. Also, fourteen heavy metals present in the dust samples were analyzed by ICP. ICP analysis showed that one of major source of the road dust would be urban construction. A large amount of silt was found, which might be originated mainly from building construction and open beds of trees. Trace element and pollution indices of heavy metals(Cd, Cu, Ni, Pb, Zn) on the roads adjacent to the commercial area had higher concentrations than those on the roads adjacent to the construction and residential areas because of traffic density and heavy traffic.

• 한국대기환경학회지
• /
• 제27권3호
• /
• pp.347-357
• /
• 2011

The TSP aerosols were collected at Gosan site of Jeju Island between 2003 and 2007, and their aerosol components were analyzed to examine the variations of chemical compositions with the corresponding pathways of inflowing air parcels. According to the comparison of seasonal aerosol compositions, the soil-originated components showed remarkably high concentrations during spring season. On the other hand, the concentrations of anthropogenic components were somewhat high in spring and summer seasons, but low in fall season. Based on the comparison of TSP compositions in relation to the pathways of inflowing air mass, the concentrations of anthropogenic components (nss-$SO_4^{2-}$, S, $NO_3^-$), soil-originated components (nss-$Ca^{2+}$, Al, Fe, Ca), and the heavy metals (e,g., Mn, Zn, Cr, Pb, Cu, Cd, etc.) have relatively increased with the air mass moving from China continent into Jeju area. Meanwhile, the marine-originated components showed an increasing trend with the air mass coming from North Pacific Ocean. In the seasonal and sectional comparison, the nss-$SO_4^{2-}$, $NO_3^-$, nss-$Ca^{2+}$, and Al showed comparatively high concentrations when the air mass moved from China continent during all seasons. Especially, the $NO_3^-$, nss-$Ca^{2+}$, and Al concentrations were somewhat high when the air mass moved from Korean Peninsula during summer season. It was also recognized that the Na+ concentration were high, when the air mass moved from Pacific Ocean through all seasons.

• 동의생리병리학회지
• /
• 제18권6호
• /
• pp.1784-1794
• /
• 2004

To study the effects between Cd inhalation toxicity and methanol extract of Radix Achyranthis Bidentatae, 4 rat groups were exposed to Cd aerosol by whole-body inhalation exposure for 6 hours/day, 5 days/week, and 4 weeks. Cd concentration in air was 0.98㎎/㎥ and mass median diameter(MMD) was 1.78㎛. 3 different dose intraperitoneal injections of methanol extract of Radix Achyranthis Bidentatae to 3 inhalation exposure groups applied for 4 weeks and the results were as follows: The highest body weight gain for 4 weeks and food intake per day were from inhalation exposure group Ⅲ(p<0.05). The highest lung weight was from inhalation exposure group Ⅲ and the highest liver and kidney weight were from inhalation exposure group Ⅱ(p<0.05). The lowest Cd content in lung was 22.77㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest Cd concentration in blood was 11.71㎍/㎗ from inhalation exposure group Ⅰ(p<0.05). Cd concentrations of 14.87㎍/g in liver and 17.91㎍/g in kidney were the highest from inhalation exposure group Ⅰ(p<0.05). The lowest Cd concentration in liver and kidney were 5.71㎍/g and 3.17㎍/g from the control(p<0.05). For weekly Cd concentration in urine, the highest value was 0.48㎍/㎖ from inhalation exposure group Ⅲ of the 3rd week and inhalation exposure group Ⅰ, Ⅱ of the 4th week. For weekly Cd concentration in feces, the highest value was 0.32㎍/g from inhalation exposure group Ⅰ, Ⅱ, Ⅲ. The highest metallothionein concentration in lung was 89.02㎍/g from inhalation exposure group Ⅲ(p<0.05). The highest metallothionein concentrations in liver and kidney were 265.47㎍/g and 214.21㎍/g from inhalation exposure group Ⅲ, respectively(p<0.05). The highest Hct, Hb, and WBC values were from inhalation exposure group Ⅱ and the highest RBC value was from inhalation exposure group Ⅲ(p<0.05). Mostly damaged part in liver tissue was hepatic lobule and the degrees of damage were lessened by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae. Proximal, distal convoluted tubules and glomerulus in kidney tissue were mostly damaged part. Degeneration and swelling were partially observed but the degrees of kidney tissue damage were lessened more or less by the intraperitoneal injection of methanol extract of Radix Achyranthis Bidentatae.

• 대한예방한의학회지
• /
• 제6권1호
• /
• pp.140-155
• /
• 2002

Background: SBP(桑白皮)is an herbal medicine which has been used in oriental medicine as a traditional therapeutic agent of bronchial asthma. Objective: This study was performed to investigate the effect of SBP on the anti-hypersensitivity and immune response in the murine of type I hypersensitivity induced by the experiment. Materials and Methods: Laboratory rats were primary sensitized with OA(ovalbumin); on day 1, rats of a Control group and Sample group (SBP group) were systemically immunized by subcutaneous injection of 1 mg OA and 300mg of Al(OH)3 in a total volume of 2ml saline. The rats of the sample group were orally administered with an SBP water extract for 14 days after primary immunization. On day 14 after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerosol containing 2%(wt/vol) OA. A day after local immunization, BAL fluid and serum were collected from the rats. Total cell, lymphocyte, CD4+ T cell, CD8+ T cell, CD4+/CD8+ ratio in the BALF, and IgE level in serum were measured and evaluated. Results: SBP showed a suppressive effect on the immune response in the rats. 1. Total cells in the BALF decreased in the SBP treated group in comparision to the control group, but statistic differences were not observed. 2. Total lymphocytes in the BALF were statistically decreased in SBP treated group in comparision to the control group. 3. CD4+ T cells in the BALF were statistically decreased in SBP treated group in comparision to the control group. 4. CD8+ T cells in the BALF were not statistically different in SBP treated group and the control group. 5. The ratio of CD4+/CD8+ in the BALF was statistically decreased in SBP treated group in comparision to the control group. 6. The IgE level in serum decreased in the SBP treated group in comparision to the control group, but statistic differences were not observed.

• 한국대기환경학회지
• /
• 제18권1호
• /
• pp.25-37
• /
• 2002

The purpose of the study was to survey the distribution patterns of inorganic elements of PM-10 in the various indoor environments and analyze the pollution patterns of aerosol in various places of indoor environment using a pattern recognition method based on cluster analysis and disjoint principal component analysis. A total of 40 samples in the indoor had been collected using mini-vol portable samplers. These samples were analyzed for their 19 bulk inorganic compounds such as B, Na, Mg, Al, K, Ca, Ti, V, Cr, Fe, Ni, Cu, Zn, As, Se, Cd, Ba, Ce, and Pb by using an ICP-MS. By applying a disjoint principal component analysis, four patterns of the indoor air pollutions were distinguished. The first pattern was identified as a group with high concentrations of PM-10, Na, Mg, and Ca. The second pattern was identified as a group with high concentrations B, Mg, At, Ca, Fe, Cu, and Ba. The third pattern was a group of sites with high concentrations of K, Zn. Cd. The fourth pattern was a group with low concentrations PM-10 and all inorganic elements. This methodology was found to be helpful enough to set the criteria standard of indoor air quality, corresponding pollutants, and classification of indoor environment categories when making an indoor air quality law.

• 한국대기환경학회지
• /
• 제15권6호
• /
• pp.727-738
• /
• 1999

Total 76 aerosol samples were collected at Sungsan in Cheju Inland by high volume air sample for 1 year, from May 1995 to April 1996, and were analyzed for major elements(Na, Mg, Ca, Al, Fe) and trace elements(Mn, Co, Ni, Zn, Cd, Pb, U) by ICP/AES and ICP/MS. This study aims to determine the concentrations of trace metals and their seasonal variations in the atmosphere of Cheju Island, where is the remote area from pollution sources and also is the midway of transport of Asian continental materials into the western North Pacific. The concentrations of Na and Mg contributed by sea-salt aerosols were similar to those in the western part of Cheju island(Kosan) and in the western coast of Korea(Mallipo). They showed the highest value in summer and the lowest in spring and winter. Crustal metals(Al, Fe, Ca, Mn, Co, U) were 2~3 times lower than those of Mallipo. These metals showed the lowest values in summer and the highest in spring. Pollution-derived metals (Zn, Cd and Pb) were 2~4 times lower than those in Malipo. Some elemental ratios in aerosols grouped by three wind directions(north-northwest, east, and south-southwest) such as Fe/Al and Pb/Zn are presented as useful tracers indicating source areas, and their differentiation may be explained by geology and fuel types of source areas.

• KSBB Journal
• /
• 제21권2호
• /
• pp.157-163
• /
• 2006

본 연구에서는 초임계 ASES 공정을 이용하여 인체내 및 피부에 여러 가지로 유익한 대표적 생리활성물질인 Vitamin-C의 불안정성을 극복하기 위하여 HP-${\beta}$-CD와의 포접복합체를 제조하여 수용액상에서의 안정성을 분석하였다. X-선 회절을 이용한 Vitamin-C와 HP-${\beta}$-CD의 결정성 분석으로 초임계 ASES 공정을 통하여 포접복합체가 용이하게 형성될 수 있음을 확인하였다. HP-${\beta}$-CD가 Vitamin-C의 안정성을 향상시키는 방법으로 이용될 수 있음을 확인하였으며, $25{\pm}0.1^{\circ}C$의 온도를 유지한 pH 7.0의 50 mM 인산완충용액 상에서 순수한 Vitamin-C, 물리적인 혼합물 및 용매증발법과 초임계 ASES 공정을 이용하여 제조된 포접복합체의 Vitamin-C 겉보기 1차 분해 속도 상수는 각각 $1.45{\times}10^{-2}h^{-1},\;1.41{\times}10^{-2}h^{-1},\;1.34{\times}10^{-2}h^{-1},\;0.20{\times}10^{-2}h^{-1}$로 초임계 ASES 공정으로 제조된 포접복합체의 경우 Vitamin-C의 안정성이 매우 크게 향상되는 것을 확인하였다.