• 센서학회지
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• 제27권4호
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• pp.237-241
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• 2018

The real-time enrichment and detection of pathogens are serious issues and rapidly evolving field of research because of the ability of these pathogens to cause infectious diseases. In general, bacterial detection is accomplished by conventional colony counting or by polymerase chain reaction (PCR) after DNA extraction. As colony counting requires considerable time to cultivate, PCR is an attractive method for rapid detection. A small number of pathogens can cause diseases. Hence, a pretreatment process, such as enrichment is essential for detecting bacteria in an actual environment. Thus, in this study, we developed a microfluidic chip capable of performing rapid enrichment of bacteria and the extraction of their genes. A lectin, i.e., Concanavalin A (ConA), which shows binding affinity to the surface of most bacteria, was coated on the surface of magnetic particles to nonspecifically capture bacteria. It was subsequently concentrated through magnetic forces in a microfluidic channel. To lyse the captured bacteria, magnetic particles were irradiated by a wavelength of 532nm. The photo-thermal effect on the particles was sufficient for extracting DNA, which was consequently utilized for the identification of bacteria. Our device will help monitor the existence of bacteria in various environmental situations such as water, air, and soil.

• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.585-590
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• 2002

The effects of a high level of sucrose on the moisture content, water activity, protein denaturation and sensory properties in Chinese-style pork jerky were investigated. The pork jerky with different levels (0, 12, 15, 18 and 21%) of sucrose was prepared. Fifteen frozen boneless pork legs from different animals were used in this trial. Sucrose is a non-reducing disaccharides and would not undergo non-enzymatic browning. Some studies pointed out that sucrose might be hydrolyzed during freezing, dehydration and storage into glucose and fructose, and cause non-enzymatic browning in meat products. The results showed that moisture content and water activity of pork jerky decreased with increase of the level of sucrose. At the same time, shear value was increased due to the reduced moisture content and water activity by osmotic dehydration. However, a higher level of sucrose had a significantly negative effect on protein solubility and extractability of myosin heavy chain of pork jerky due to non-enzymatic browning. From the results of sensory panel tests, the pork jerky with 21% of sucrose seems to be more acceptable by the panelists in hardness, sweetness and overall acceptability.

• 한국환경과학회지
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• 제16권2호
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• pp.227-232
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• 2007

Water pollution in enclosed water bodies such as lake and river has become a serious problem over the world. Domestic wastewater is responsible for more than 60 % pollution load in public water area in Korea. Effluent of the treated domestic wastewater at low removal level is abundantly fed rivers and lakes and thus be an serious cause of lake pollution. Therefore, effective implement of domestic wastewater treatment in basin of lake and river must be prepared. The septic tank is one of the effective domestic wastewater treatment equipment and used in individual treatment for a unit of household, The purpose of septic tank as biological treatment system is simultaneously to remove BOD, T-N, T-P and reduce turbidity from influent. Accordingly, the appropriate control of functional microorganisms is important subject for the establishment of stability and economy of the biological treatment method. Especially, microanimals as a high-ranked microorganisms of food-chain are important, because microanimals control the other microorganisms especially various bacteria and effect on function of treatment. Therefore, it is necessary that functional predator like rotifers are attached in wastewater treatment process. In this study, the methods for attachment high density the rotifer to and improvement of transparency in the effluence by a dense rotifer was examined using laboratory scale biological treatment reactor simulated septic tank and real one.

• Journal of Information Technology Applications and Management
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• 제22권2호
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• pp.43-70
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• 2015

Concerns have been growing about whether domestic internet protocol television (IPTV) can establish a solid foothold in the pay TV market, largely because of the lack of IPTV-only content and service differentiation. It has been difficult for IPTV providers to attract valuable PPs (program providers) to strengthen their positions, and IPTV providers have invested much money into procuring content. To survive this difficult situation, IPTV providers need to reappraise their profit sharing methods and content distribution structure to facilitate the expansion of their subscriber base. This can be done by attracting valuable PPs to IPTV providers and securing extra revenue by distributing more content for their PP partners. The IPTV industry has a different structure and value chain from the digital cable industry. Moreover, profit sharing schemes among participants in the IPTV industry are complicated. Thus it is essential to analyze the criteria for profit sharing, the selection of attributes in profit sharing, and their cause-effect relationship in developing fair pricing for broadcast content in the IPTV industry. This study introduces the attributes that need to be considered for the pricing of content and profit sharing among IPTV providers and PPs. In addition, this study uses system dynamics to analyze the relationship among those attributes along with additional associated factors for the pricing of content.

• Genomics & Informatics
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• 제20권1호
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• pp.7.1-7.13
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• 2022

2-Methoxy-1,4-naphthoquinone (MNQ) has been shown to cause cytotoxic towards various cancer cell lines. This study is designed to investigate the regulatory effect of MNQ on the key cancer genes in mitogen-activated protein kinase, phosphoinositide 3-kinase, and nuclear factor κB signaling pathways. The expression levels of the genes were compared at different time point using polymerase chain reaction arrays and Ingenuity Pathway Analysis was performed to identify gene networks that are most significant to key cancer genes. A total of 43 differentially expressed genes were identified with 21 up-regulated and 22 down-regulated genes. Up-regulated genes were involved in apoptosis, cell cycle and act as tumor suppressor while down-regulated genes were involved in anti-apoptosis, angiogenesis, cell cycle and act as transcription factor as well as proto-oncogenes. MNQ exhibited multiple regulatory effects on the cancer key genes that targeting at cell proliferation, cell differentiation, cell transformation, apoptosis, reduce inflammatory responses, inhibits angiogenesis and metastasis.

• 대한안전경영과학회지
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• 제6권1호
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• pp.157-172
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• 2004

The results of this study has been split into two components, On set of results addressed the strategy of developing a Agility for maintenance consumer. The other set of results addressed the strategy of illuminating the successful measurement and concepts formed during the maintenance problem solving process, especially in the context of the Logistics Information system by aggregation information space. This paper presented a classifiable elements of Agility & Measurement of logistics capability in the Korea market. These concepts are positioned in a framework to give a better understanding what the consequences are of the changing business environment. The developments towards more globalization which leads to centralization and mass individualization that has a tendency to go more in the decentralized solutions, seems to be in contradiction. we will focus a proposal of Logistics information system and demand management decisions that should be a prime concern of any profit maximizing firm, unready strategy of investment. and we will prove the facts that it could be a guiding company which has a ability of cooperation with entities through the founding of supply chain, On the conclusion, we will show the variation which influences for capacity entities and alternative proposal to define a element which influnce for a cause and effect basically.[5]

• 한국게임학회 논문지
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• 제9권3호
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• pp.53-66
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• 2009

MMORPG에서의 NPC(Non Player Character)는 게임 서사에서 갈등을 유발하고 극적 사건을 형성하는데 중추적 역할을 수행한다. 본 연구에서는 MMORPG 를 중심으로 NPC의 유형과 그에 따른 서사적 기능을 분석했다. NPC는 플레이어 캐릭터와의 관계축, 행동의 태도축에 따라서 크게 소극적 조력자, 적극적 조력자, 소극적 적대자, 적극적 적대자로 분류된다. NPC는 유형에 따라서 변별적인 서사적 기능을 수행한다. 가령 소극적 조력자는 공간이동과 서사 연쇄의 구심점으로 기능하며, 적극적 적대자는 공간 투시를 유도하고 극적 효과를 창출한다. 이처럼 MMORPG에서 NPC는 게임 서사 진행에 있어서 주요한 서사적 기능을 수행한다. 표면적으로 NPC는 게임 서사에 있어서 평면적 제한적 기능을 수행하지만, 이면적으로는 플레이어와 상호작용하는 가운데 입체적 확장적 기능을 수행하면서 다선형적인 게임 서사를 생성한다는 점에서 의의가 있다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 1999년도 춘계학술대회 논문집
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• pp.357-361
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• 1999

GOD electrochemically immobilized in PPy/GOD complex have an effect on redox properties of the complex. In the cyclicvoltammetry, GOD shows the redox reaction at the potential below -0.6Y vs. Ag/AgCI. That leads to new peaks in the cyclicvoltammograms in additional to typical PPy peaks. The pH of electrolyte solution during potential swing decreased to 4.4, and then increased to 10. That suggests the redox of GOD for the cycling. As the concentration of GOD was increased, the anodic wave of the new peaks was strong as much as increased. GOD obstructs the diffusion of electrolyte anion because of its net chain. Insulating property of GOD is cause that it made the faradic impedance of complex large in charge transfer. It suggests that increase of the concentration of GOD be against electrochemical coupling. Therefore, the concentration of GOD and electrochemical coupling should be dealt with each other. The apparent Michaelis-lenten constant ( K\`$_{M}$ ) was determined by 30.7 mmol d $m^{-3}$ fur the PPy/GOD complex. The value is of the same order of magnitude as that for soluble glucose oxidase from Aspergillus Niger.r.

• 인간식물환경학회지
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• 제24권1호
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• pp.53-62
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• 2021

Background and objective: Dermatitis is a chronic disease accompanied by such symptoms as itching and dry skin. The environment and diet can aggravate dermatitis, so attention to skin care is essential. Colocasia esculenta is used in various manners and for different purposes, including with regard to inflammation, aging, and the digestive system. The anti-inflammatory effect of Colocasia esculenta water extract was confirmed using RAW 264.7 macrophages with regard to male ICR mice. Methods: In the case of the ICR mice, 5% 12-O-Tetradecanoylphorbol-13-acetate (TPA) was used to cause inflammation for 7 days, and 100 μL of Colocasia esculenta water extract and panthenol were administered orally for 10 days. In addition, RT-PCR, NO, ELISA was conducted. Results: As a result of reverse transcription polymerase chain reaction (RT-PCR) using RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS), it was found that Colocasia esculenta water extract reduced the expression of inflammatory cytokines. As a result of hematoxylin and eosin (H&E) staining using mouse ear tissue, Colocasia esculenta water extract reduced ear thickness and showed an effect of suppressing ear edema. In addition, compared to the TPA-treated group, the Colocasia esculenta extract-treated group had reduced nitric oxide (NO) production by 18.23 μM and IL-13 production decreased by 136.55 pg/ml. Conclusion: Colocasia esculenta water extract has been shown to be effective in lowering inflammatory cytokine production. These results suggest that Colocasia esculenta water extracts can be used as natural products to treat dermatitis.

• 통합자연과학논문집
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• 제10권2호
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• pp.85-94
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• 2017

Fibroblast growth factor receptor (FGFR) belongs to the family of receptor tyrosine kinase. They play important roles in cell proliferation, differentiation, development, migration, survival, wound healing, haematopoiesis and tumorigenesis. FGFRs are reported to cause several types of cancers in humans which make it an important drug target. In the current study, HQSAR analysis was performed on a series of recently reported 1H-Pyrazolo [3,4-b]pyridine derivatives as FGFR antagonists. The model was developed with Atom (A) and bond (B) connection (C), chirality (Ch), hydrogen (H) and donor/acceptor (DA) parameters and with different set of atom counts to improve the model. A reasonable HQSAR model ($q^2=0.701$, SDEP=0.654, NOC=5, $r^2=0.926$, SEE=0.325, BHL=71) was generated which showed good predictive ability. The contribution map depicted the atom contribution in inhibitory effect. A contribution map for the most active compound (compound 24) indicated that hydrogen and nitrogen atoms in the side chains of ring B as well as hydrogen atoms in the side chain of ring C and the nitrogen atom in the ring D contributed positively to the activity in inhibitory effect whereas, the lowest active compound (compound 04) showed negative contribution to inhibitory effect. Thus results of our study can provide insights in the designing potent and selective FGFR kinase inhibitors.