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Identification, Enzymatic Activity, and Decay Ability of Basidiomycetous Fungi Isolated from the Decayed Bark of Mongolian Oak (Quercus mongolica Fisch. ex Ledeb.)

  • Nguyen, Manh Ha;Kim, Dae Ho;Park, Ji Hyun;Park, Young Ui;Lee, Moo Yeul;Choi, Myeong Hee;Lee, Dong Ho;Lee, Jong Kyu
    • Journal of Forest and Environmental Science
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    • v.37 no.1
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    • pp.52-61
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    • 2021
  • Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.

Influence of (E)-2-hexenyl (Z)-3hexenoate on the Occurrence of Egg Parasitoids, Ooencyrtus nezarae (Hymenoptera: Encyrtidae) and Gryon japonicum (Hymenoptera: Scelionidae), in Apple Orchards (사과원에서 (E)-2-hexenyl (Z)-3hexenoate 성분이 노린재깡충좀벌(Ooencyrtus nezarae)(Hymenoptera: Encyrtidae)과 노린재기생알벌(Gryon japonicum) (Hymenoptera: Scelionidae) 발생에 미치는 영향)

  • Baek, Seonghoon;Park, Chung Gyoo;Seo, Bo Yoon;Cho, Jum Rae;Park, Chang-Gyu
    • Korean journal of applied entomology
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    • v.60 no.1
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    • pp.91-98
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    • 2021
  • The populations of Riptortus pedestris (Fabricius) cause serious economic damages in local apple orchards. However, there was no study to manage its populations with environment friendly tactics. Thus, this study was conducted to analyze its attraction effects of one of R. pedestris aggregation pheromone compounds, (E)-2-hexenyl (Z)-3hexenoate (E2HZ3H), to its parasitoids, Ooencyrtus nezarae (Ishii) and Gryon japonicum (Ashmead). This compound did not increase the number of these two parasitoids and change its spatial distribution. However, this compound could attract two parasitoids within limited distance. These results indicates that one compound of R. pedestris aggregation pheromone, E2HZ3H, could be a good candidate to manage R. pedestris populations in conditions that its populations locally occurs in apple orchards.

OmpR Is Essential for Growth and Expression of Virulence-related Genes in the Fish Pathogen Edwardsiella piscicida (어류 병원체 Edwardsiella piscicida의 OmpR은 생육과 병원성과 관련된 유전자의 발현에 필수적)

  • Ray, Durga;Kim, Yeon Ha;Choe, unjeong;Kang, Ho Young
    • Journal of Life Science
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    • v.31 no.1
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    • pp.28-36
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    • 2021
  • Edwardsiella piscicida is a significant cause of hemorrhagic septicemia in fish and gastrointestinal infections in humans. Survival bacteria require specialized mechanisms to adapt to environmental fluctuations. Hence, to understand the mechanism through which E. piscicida senses and responds to environmental osmolarity changes, we determined the protein expression profile and physiological properties under various salinity conditions in this study. The OmpR protein is a part of the Env-ZOmpR two-component system that has been implicated in sensing salt stress in bacteria. However, the physiological role played by this protein in E. piscicida remains to be elucidated. Therefore, in this work, the function of the OmpR protein in response to salt stress was investigated. Phenotypic analysis revealed that, in the mutant, three of the biochemical phenotypes were different from the wild type, including, citrate utilization, hydrogen sulfide, and indole production. Introduction of the plasmid containing the entire ompR gene to the mutant strain returned it to its parental phenotype. The retarded growth rate also partially recovered. Furthermore, in our studies, OmpR was not found to be related to cell motility. Taken together, our results from the mutational analysis, the growth assay, MALDI-TOF MS, qRT-PCR, and the phenotype studies suggest that the OmpR of E. piscicida is implicated in osmoregulation, growth, expression of porins (ETAE_1826), virulence-related genes (EseC, EseD and EvpC), and certain genes of unknown function (ETAE_1540 and ETAE_2706).

A Study on the Optimal Setting of Large Uncharged Hole Boring Machine for Reducing Blast-induced Vibration Using Deep Learning (터널 발파 진동 저감을 위한 대구경 무장약공 천공 장비의 최적 세팅조건 산정을 위한 딥러닝 적용에 관한 연구)

  • Kim, Min-Seong;Lee, Je-Kyum;Choi, Yo-Hyun;Kim, Seon-Hong;Jeong, Keon-Woong;Kim, Ki-Lim;Lee, Sean Seungwon
    • Explosives and Blasting
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    • v.38 no.4
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    • pp.16-25
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    • 2020
  • Multi-setting smart-investigation of the ground and large uncharged hole boring (MSP) method to reduce the blast-induced vibration in a tunnel excavation is carried out over 50m of long-distance boring in a horizontal direction and thus has been accompanied by deviations in boring alignment because of the heavy and one-directional rotation of the rod. Therefore, the deviation has been adjusted through the boring machine's variable setting rely on the previous construction records and expert's experience. However, the geological characteristics, machine conditions, and inexperienced workers have caused significant deviation from the target alignment. The excessive deviation from the boring target may cause a delay in the construction schedule and economic losses. A deep learning-based prediction model has been developed to discover an ideal initial setting of the MSP machine. Dropout, early stopping, pre-training techniques have been employed to prevent overfitting in the training phase and, significantly improved the prediction results. These results showed the high possibility of developing the model to suggest the boring machine's optimum initial setting. We expect that optimized setting guidelines can be further developed through the continuous addition of the data and the additional consideration of the other factors.

Risk assessment of Staphylococcus aureus infection in ready-to-eat Samgak-Kimbap (즉석섭취 삼각김밥에서의 Staphylococcus aureus 위해평가 연구)

  • Lee, Chae Lim;Kim, Yeon Ho;Ha, Sang-Do;Yoon, Yo Han;Yoon, Ki Sun
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.661-669
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    • 2020
  • Samgak-Kimbap is a popular ready-to-eat (RTE) food at convenience stores, in Korea. Although Samgak-Kimbap is distributed through the cold chain supply system, inappropriate temperature storage conditions prior to consumption are a cause of concern. The objective of this study was to evaluate the risk of Staphylococcus aureus growth in Samgak-Kimbap in the retail market. The prevalence and contamination levels of S. aureus in Samgak-Kimbap (n=170) were monitored, and the predictive growth model of a five-strain cocktail of enterotoxin-producing S. aureus (SEA, SEB, SEC, SED, and SEE) was developed in Samgak-Kimbap as a function of temperature (4, 10, 11, 20, 25, and 37℃). We could not observe the growth of S. aureus and enterotoxin-producing S. aureus in Samgak-Kimbap at temperatures below 10℃. The probability of illness with S. aureus per serving of Samgak-Kimbap was 1.44×10-10 per day. The most influential factor in increasing the risk of foodborne illnesses was the contamination level of S. aureus in Samgak-Kimbap.

Psychological Essentialism and Category Representation (심리적 본질주의와 범주표상)

  • Kim, ShinWoo;Jo, Jun-Hyoung;Li, Hyung-Chul O.
    • Korean Journal of Cognitive Science
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    • v.32 no.2
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    • pp.55-73
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    • 2021
  • Psychological essentialism states that people believe some categories to have hidden and defining essential features which cause other features of the category (Gelman, 2003; Hirschfeld, 1996; Medin & Ortony, 1989). Essentialist belief on categories questions the Roschian argument (Rosch, 1973, 1978) that categories merely consist of clusters of correlated features. Unlike family resemblance categories, essentialized categories are likely to have clear between-category boundaries and high within-category coherence (Gelman, 2003; Prentice & Miller, 2007). Two experiments were conducted to test the effects of essentialist belief on category representation (i.e., between-category boundary, within-category coherence). Participants learned family resemblance and essentialized categories in their assigned conditions and then performed categorization task (Expt. 1) and frequency estimation task of category exemplars (Expt. 2). The results showed, in essentialized categories, both boundary intensification and greater category coherence. Theses results are likely to have arisen due to increased cue and category validity in essentialized categories and suggest that essentialist belief influences macroscopic representation of category structure.

Analysis of microplastics released from textiles according to filter pore size and fabric weight during washing (세탁 중 세탁물 중량과 여과 기공 크기에 따른 미세플라스틱 분석)

  • Choi, Sola;Kwon, MiYeon;Park, Myung-Ja;Kim, Juhea
    • Journal of the Korea Fashion and Costume Design Association
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    • v.23 no.1
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    • pp.37-45
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    • 2021
  • This study observed the release of microplastics according to washing weights and filtering conditions, measured microplastic generation rates, fiber lengths, and fiber diameters. This study attempted to present data for the development of filters that decrease microplastic generation. For test samples, polyester piled knit fabric (cut-pile) was selected, which currently has the highest amount of consumption in the clothing industry, but can easily cause marine pollution because of its low biodegradability. For test equipment, a drum washer was used and microplastics were collected using two filter pore sizes, 5 ㎛ and 20-25 ㎛. Microplastic fibers weights and lengths were measured. The results of the experiment showed the following: 1) The release of microplastics differed according to the fabric weights and washing process; 2) washing fabric weights showed a differences in the collection amount according to the filter pore size (5 ㎛, 20-25 ㎛); 3) observations of differences in the lengths of the microplastics that occur during the washing process by filter pore size were made. Fibers with shorter lengths appeared with filter pore sizes of 5㎛ in comparison to filter pore sizes of 20-25㎛. The results from this study on microplastic generation by fabric during washing, demonstrated the following conclusions that can be used to reduce the release of microplastics. First, the release of microplastics according to fabric weights and washing courses are affected by physical force. Therefore, it is necessary to reduce the amount of physical force due to water flow, increase the fabric weight, or wash the material in low temperatures. Second, in the manufacturing of washing machines, microplastic filtration can be promoted or legislatation supporting microplastic filtration can be introduced.

The Effect of Shoe Heel Types and Gait Speeds on Knee Joint Angle in Healthy Young Women - A Preliminary Study

  • Chhoeum, Vantha;Wang, Changwon;Jang, Seungwan;Min, Se Dong;Kim, Young;Choi, Min-Hyung
    • Journal of Internet Computing and Services
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    • v.21 no.6
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    • pp.41-50
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    • 2020
  • The consequences of wearing high heels can be different according to the heel height, gait speed, shoe design, heel base area, and shoe size. This study aimed to focus on the knee extension and flexion range of motion (ROM) during gait, which were challenged by wearing five different shoe heel types and two different self-selected gait speeds (comfortable and fast) as experimental conditions. Measurement standards of knee extension and flexion ROM were individually calibrated at the time of heel strike, mid-stance, toe-off, and stance phase based on the 2-minute video recordings of each gait condition. Seven healthy young women (20.7 ± 0.8 years) participated and they were asked to walk on a treadmill wearing the five given shoes at a self-selected comfortable speed (average of 2.4 ± 0.3 km/h) and a fast speed (average of 5.1 ± 0.2 km/h) in a random order. All of the shoes were in size 23.5 cm. Three of the given shoes were 9.0 cm in height, the other two were flat shoes and sneakers. A motion capture software (Kinovea 0.8.27) was used to measure the kinematic data; changes in the knee angles during each gait. During fast speed gait, the knee extension angles at heel strike and mid-stance were significantly decreased in all of the 3 high heels (p<0.05). The results revealed that fast gait speed causes knee flexion angle to significantly increase at toe-off in all five types of shoes. However, there was a significant difference in both the knee flexion and extension angles when the gait in stiletto heels and flat shoes were compared in fast gait condition (p<0.05). This showed that walking fast in high heels leads to abnormal knee ROM and thus can cause damages to the knee joints. The findings in this preliminary study can be a basis for future studies on the kinematic changes in the lower extremity during gait and for the analysis of causes and preventive methods for musculoskeletal injuries related to wearing high heels.

The Effects of Different Surface Level on Muscle activity of the Upper Body and Exercise Intensity during Mountain Climbing Exercise (지면에서의 마운틴 클라이밍 운동 시 상체의 위치 변화가 운동 강도와 근활성도에 미치는 영향)

  • Park, Jun-Ho;Jung, Jae-Hu;Kim, Jong-Geun;Chae, Woen-Sik
    • Korean Journal of Applied Biomechanics
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    • v.31 no.1
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    • pp.72-78
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    • 2021
  • Objective: The purpose of this study was to investigate relations and effectiveness about mountain climbling exercise with different level of support surfaces by analyzing heart rate and EMG data. A total of 10 male college students with no musculoskeltal disorder were recruited for this study. Method: The biomechanical analysis was performed using heart rate monitor (Polar V800, Polar Electro Oy, Finland), step-box, exercise mat, and EMG device (QEMG8, Laxtha Inc. Korea, sampling frequency = 1,024 Hz, gain = 1,000, input impedance > 1012 Ω, CMRR > 100 dB). In this research, step-box were used to create different surface levels on the upper body (flat surface, 10% of subject's height, 20% of subject's height, and 30% of subject's hight). Based on these different conditions, data was collected by performing mountain climbing exercise during 30 seconds. Subjects were given 5 minutes of break to prevent muscular fatigue after each exercise. For each dependent variable, a one-way analysis of variance with repeated measures was conducted to find significant differences and Bonferroni post-hoc test was performed. Results: The results of this study showed that exercise intensity was reduced statistically as increased surface level on the upper body. Muscle activity of the upper rectus abdominis and biceps femoris for 30% of surface level was significantly higher than the corresponding values for flat surface. However, the opposite was found in the rectus femoris. In general, muscle activity of the lower rectus abdominis, erector spinae, external oblique abdominis, and gluteus maximus increased when surface level increased, but the differences were not significant. Conclusion: As a result, the increase in surface level of the body would change muscle activity of the upper body, indicating that different surface level of the upper body may cause significant effect on particular muscles to be more active during mountain climbing exercise. Based on results of this study, it is suggested to set up an appropriate surface level to target particular muscle to expect an effective training. It is also important to set adequate surface levels to create an effective training condition for preventing exercise injuries.

Exploring the Thalamus of the Human Brain using Tractography Analysis at 3Tesla MRI (3 Tesla MRI에서 트랙토그래피 분석을 이용한 시상 탐색)

  • Im, Sang-Jin;Kim, Joo-Yeon;Baek, Hyeon-Man
    • Journal of the Korean Society of Radiology
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    • v.15 no.4
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    • pp.555-564
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    • 2021
  • Thalamus is known to play an important role in the regulation of nerve function. Thalamus, located in the center of the brain, is involved in sleep, arousal, and emotional regulation, and has been reported to be associated with multiple sclerosis, essential tremors, and neurodegenerative diseases such as Parkinson's disease. In addition, it has been reported that iron deposits in the thalamus can cause depressive symptoms with age. Although there are discrepancies between studies, it can be deduced that the thalamus region has a clear effect on neurological disorders due to a strong relationship between the thalamus and neurological functions such as emotional control and processing. Through tractography analysis, the connectivity between the detailed areas of each subcortical region was investigated in the form of a matrix, showing strong connectivity and weak interhemispheric connectivity. In the 59> group, the WM connectivity of thalamus was found to be weaker than those of the two groups. Comparisons between the two groups showed that the young groups (10-39 and 40-59) had higher connection intensity than the 59> group and that statistically significant differences in 3 connection pathways were found in each hemisphere. A decrease in thalamus-related connection strength in aging has shown that it can affect emotional and neurological disorders such as anxiety and depression, and network measurements can help assess cognitive impairment across clinical conditions.