• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.594-597
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• 2014

본 논문에서는 국제 태양광 자동차 대회를 참가하는 태양광 자동차 프론트 너클의 경량화 설계에 관한 연구를 진행한다. 이를 위해 Cattle grid 를 포함한 실제 주행환경과 태양광 자동차를 동역학 시뮬레이션 모델로 구성하고 대회에서 차량의 평균속도인 70Km/h 로 주행 시, 서스펜션에서 발생되는 동하중을 측정하였다. 프론트 너클을 유한요소로 구성하고 다물체 동역학 시뮬레이션에서 도출된 하중들로 위상최적기법을 통해 프론트 너클의 경량화를 이루었다. 마지막으로 피로해석을 수행하여 그 타당성을 검증하였다.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.1
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• pp.13-18
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• 1992

Three cattle, a sheep and a goat were slaughtered to determine the distribution of digesta particles and mean size of digesta particles. Aliquot samples of digesta in the diverse sites of the digestive tracts were fractionated by a wet sieving technique. Fractionated particles were analyzed by the magnetic grid analyzer system constructed by authors. Results showed that the proportion of particles in digesta was similar among the omasum, abomasums cecum, colon and rectum, but that for the reticulo-ruminal digesta was different from the others. The pattern of the mass base frequency distribution of particles was also similar in the post-ruminal digesta. Average Heywood's diameter (the diameter equivalent to that of a circle with equal area to a projected area of a given particle) was about 1.2 mm in the reticulo-ruminal digesta and decreased to 0.65 mm for cattle or to about 0.35 mm for sheep and goat in the omasal digesta. Average Heywood's diameter was about the same in the post-ruminal digesta. It is concluded that mean particle size and particle distribution in digesta of the rectum or feces reflect those in digesta of the omasum.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.900-904
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• 2002

The duration of spatial memory in cattle is potentially important for grazing management. The ability of livestock to remember the location of food patches may lead to uneven range use. In this experiment, how long cattle are able to remember food locations was determined. Six steers were used to conduct this study in a pasture with an 8 row by 8 column grid of 64 plastic containers 5 meters apart. Four randomly chosen containers were loaded with feed pellets. All steers were trained to find the loaded locations until a minimum of empty containers were visited. After this initial training, each steer was tested at 5, 10, 20 and 48 days post-training. Total number of visits (TV), number of containers visited (NC), the ratio of loaded containers (LC) to NC were recorded. Once the steers learned the locations of loaded containers NC did not increase with time since last training up to 48 days (p>0.05). Logarithmic transformation of NC (LNC) was 0.70 and 0.80 for the control and 48 day treatments, respectively. Steers were equally efficient in locating containers with feed. The steers also showed that their ability in locating food was much better than expected by chance (Z>1.62). Findings of the present study do not suggest using spatial memory decay as a tool to promote better grazing distribution. Because steers remembered food locations accurately for at least 48 days.

• Journal of Embryo Transfer
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• v.13 no.2
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• pp.191-197
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• 1998

Immature nocytes and in VitrO matured Oocytes collected from the slaughtered Korean cattle were frozen slowly with 10% ethylene glycol+5% polyvinyl pyrolidine+0.05M trehalose (l0EPT), 10% ethylene glycol+5% ficoll+0.05M sucrose (1OEFS), or 10% ethylene glycol+5% ficoll+0.05M trehalose (l0EFT) by cell freezer (experiment 1). And also,They were ultra-rapidly frozen with 30% ethylene glycol+10% polyvinyl pyrolidine+0.5M trehalose (3OEPT) or 30% ethylene glycol+18% ficoll+0.5M sucrose (3OEFS) using electron microscope grid (experiment 2). In experiment 1, the cleavage rate was 23.0% when immature oocytes were frozen slowly using various cryoprotectants descrihed above, and 5.1% of cleaved oocytes developed to over morula stage after in Vitro fertilization (IVF). There were no significant differences among these groups. When matured oocytes were frozen slowly, the total cleavage rate was 19.7%, and over morula stage was 3.2%. lOEPT (4.8%) and EFS (4.4%) were slightly more effective than l0EFT (0.0%) for development in vitro. Only in l0EFT treated group, immature oocytes have higher developmental capacity than matured ones, when they were frozen slowly and IVF after thawing. In experiment 2, oocytes were ultra-rapidly frozen using the electron microscope grid with two kind of cryoprotectants described above. In immature oocyte group, the cleavage rate was 13.9% and 5.8% of cleaved oocytes developed to over morula stage after IVF, and in matured group, 25.7 and 7.6%, respectively. There were no significant differences between two kind of cryoprotectants, but in ultra-rapid freezing using electron microscope grid, the efficiency is slightly higher in matured oocyte group.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.393-400
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• 2021

Body weight of livestock is a crucial indicator for assessing feed requirements and nutritional status. This study was performed to estimate the body weight of Korean cattle (Hanwoo) using body volume determined from three-dimensional (3-D) image. A TOF camera with a resolution of 640×480 pixels, a frame rate of 44 fps and a field of view of 47°(H)×37°(V) was used to capture the 3-D images for Hanwoo. A grid image of the body was obtained through preprocessing such as separating the body from background and removing outliers from the obtained 3-D image. The body volume was determined by numerical integration using depth information to individual grid. The coefficient of determination for a linear regression model of body weight and body volume for calibration dataset was 0.8725. On the other hand, the coefficient of determination was 0.9083 in a multiple regression model for estimating body weight, in which the age of Hanwoo was added to the body volume as an explanatory variable. Mean absolute percentage error and root mean square error in the multiple regression model to estimate the body weight for validation dataset were 8.2% and 24.5kg, respectively. The performance of the regression model for weight estimation was improved and the effort required for estimating body weight could be reduced as the body volume of Hanwoo was used. From these results obtained, it was concluded that the body volume determined from 3-D of Hanwoo could be used as an effective variable for estimating body weight.

• Korean Journal of Animal Reproduction
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• v.23 no.4
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• pp.281-291
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• 1999

The objective of this study was to optimize the freezing/thawing method of in vitro produced Hanwoo blastocysts. Day 7 blastocysts after IVF were vitrified using EFS40 (40% ethylene glycol, 18% ficoll, 0.3 M sucrose and 10% FBS added m-DPBS) as a freezing solution and electron microscope (EM) grid (V-G) or straw (V-S) as an embryo container. In both method, freezing/thawing were treated by 2-step, treatment time was required in V-G method and V-S method, for 2 min / 3 min and 3.5 min / 10 min, respectively. Embryo survival was assessed as re-expanded and hatched rates at 24 h and 48 h after warming, respectively. The results obtained in these experiments were summarized as follows: when the effect of exposure in vitrification solution and chilling injury from freezing procedure on in vitro produced expanded blastocysts were examined, at 24 h after warming, embryo survival in exposure group (100.0%) was not different compared to that in control group (100.0%), although those results were significantly different with two vitrified groups (V-G: 87.8, V-S: 77.8%) (P＜0.001). However, at 48 h after warming, hatched rates of V-G group (67.8%) were significantly higher than those of V-S group (53.3%) (P＜0.05). In addition, this hatched rate in V-G group was not different with that in exposure group (73.3%). When the effects of embryo developmental stage (early, expanded and early hatching blastocysts) and embryo container (EM grid and straw) to the in vitro survival of vitrified-warmed day 7 Hanwoo blastocysts were simultaneously examined, fast developed embryos were indicated the better resistance to freezing than delayed developed one, irrespective of embryo containers (early; 57.1 ＆ 24.4%, expanded; 84.7 ＆ 60.6%, early hatching; 91.7 ＆ 80.0%) (P＜0.001). Especially, in expanded and early hatching blastocysts, embryo survival of V-G group (67.8, 95.0%) was significantly higher than those of V-S group (53.0, 65.0%) at 48 h post warming, respectively (P＜0.05, P＜0.001). Therefore, this study indicates that Hanwoo blastocysts can be cryopreserved more simple, efficient and successful by vitrification method using EM grid.