• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.467-476
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• 2019

Objective: This research was conducted to study the genetic diversity in several Indonesian cattle breeds using microsatellite markers to classify the Indonesian cattle breeds. Methods: A total of 229 DNA samples from of 10 cattle breeds were used in this study. The polymerase chain reaction process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain the observed number of alleles, effective number of alleles, observed heterozygosity value, expected heterozygosity value, allele frequency, genetic differentiation, the global heterozygote deficit among breeds, and the heterozygote deficit within the breed, gene flow, Hardy-Weinberg equilibrium, and polymorphism information content values. The MEGA program was used to generate a dendrogram that illustrates the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and analysis of molecular variance. The principal component analysis (PCA) was performed using adegenet package of R program. Results: A total of 862 alleles were detected in this study. The INRA23 allele 205 is a specific allele candidate for the Sumba Ongole cattle, while the allele 219 is a specific allele candidate for Ongole Grade. This study revealed a very close genetic relationship between the Ongole Grade and Sumba Ongole cattle and between the Madura and Pasundan cattle. The results from the CFA, PCoA, and PCA analysis in this study provide scientific evidence regarding the genetic relationship between Banteng and Bali cattle. According to the genetic relationship, the Pesisir cattle were classified as Bos indicus cattle. Conclusion: All identified alleles in this study were able to classify the cattle population into three clusters i.e. Bos taurus cluster (Simmental Purebred, Simmental Crossbred, and Holstein Friesian cattle); Bos indicus cluster (Sumba Ongole, Ongole Grade, Madura, Pasundan, and Pesisir cattle); and Bos javanicus cluster (Banteng and Bali cattle).

• Animal Bioscience
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• v.37 no.4
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• pp.591-599
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• 2024

Objective: Sumba Ongole (SO) cattle are valuable breed due to their important role in the development of Indonesian cattle. Despite rapid advances in molecular technology, no genomic studies on SO cattle have been conducted to date. The aim of this study is to provide genomic profile related to the population diversity, admixture, and demographic trends of SO cattle. Methods: Genomic information was gathered from 79 SO cattle using the Illumina Bovine SNP50 v3 Beadchip, and for comparative purposes, additional genotypes from 209 cattle populations worldwide were included. The expected and observed heterozygosity, inbreeding coefficient, pairwise fixation indices between-population, and Nei's genetic distance were examined. Multidimensional scaling, admixture, and treemix analyses were used to investigate the population structure. Based on linkage disequilibrium and effective population size calculations, the demographic trend was observed. Results: The findings indicated that the genetic diversity of SO cattle was similar to that of other indicine breeds. SO cattle were genetically related to indicines but not to taurines or Bali cattle. The study further confirmed the close relationship between SO, Ongole, and Nellore cattle. Additionally, a small portion of the Ongole mixture were identified dominant in the SO population at the moment. The study also discovered that SO and Bali cattle (Bos javanicus) could have been ancestors in the development of Ongole Grade cattle, which corresponds to the documented history of Ongolization. Our finding indicate that SO cattle have maintained stability and possess unique traits separate from their ancestors. Conclusion: In conclusion, the genetic diversity of the SO cattle has been conserved as a result of the growing significance of the present demographic trend. Consistent endeavors are necessary to uphold the fitness of the breed.

• Journal of Veterinary Clinics
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• v.6 no.1
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• pp.193-198
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• 1989

This study was performed to determine the blood chemical values of the 50 normal multiparous and 98 infertile dairy cattle which were raised in the high-land of kangweon area. The results obtained in this study were summerized as follow. 1. The mean values of the serum total protein, albumin and A/G ratio the infertile dairy cattle were lower than those of the normal multiparous dairy cattle. 2. The mean value of the serum globulin of the infertile dairy cattle was higher than that of the normal multiparous dairy cattle. 3. The mean values of the serum total cholesterol, glucose, calcium and inorganic phosphorus of the infertile dairy cattle were lower than those of the normal multiparous dairy cattle. 4. The mean value of the SGOT of the Infertile dairy cattle was higher than that of the normal multiparous dairy cattle.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.1
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• pp.81-86
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• 1996

Silage wm(cv, Gwanganok) was cultivated during 1991-1994 on paddy land as a rotational cropping system of rice, to evaluate the effect of cattle manure application on the soil characteristics, yield performance and quality of com plant. The treatments used in this study were non-fertilizer, NPK standard in chemical fertilizer(N:$P_2O_5$ : $K_2O$= 200 : 150 : 150 kg/ha), cattle manure 20.40, 60 and 80 ton/ha Application of cattle manure improved soil pH, organic matter, available phosphate and exchangeable cations in the soils. During the experiment, soil pH was improved from 4.7 in the chemical fertilizer application(control) to 5.4-5.6 in the application of cattle manure, and available $P_2O_5$ wntent was increased from 72.2 ppm(contro1) to 340.2 ppm(cattle manure 80 ton/ha). Application of cattle manure increased plant growth, plant height and stalk diameter, and silage yields. Dry matter yields were produced 15.88 ton(chemical fertilizer), 20.11 ton(cattle manure 40 ton) and 21.22 ton/ha(cattle manure 80 ton/ha). However, no signicant yield differences were observed between cattle manure 40, 60 and 80 tonlha Productions of total digestible nutrients(TDN) and net energy for lactation(NEL) were also increased under cattle manure application. From the abobe results, the proper application amount of cattle manure was 40 tonha in this experiment.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.8
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• pp.1048-1052
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• 2011

The Chinese Holstein cattle breed, an introduced breed in China, has been crossbred with native cattle breeds. We hypothesised that the Chinese Holstein local population in Beijing share haplotypes with native Asian cattle breeds, the result of a sudden population expansion in the recent past. We also hypothesised that crossbreeding and population expansion left traces that shaped the genetic makeup of the breed. Evaluation of this was performed by mitochondrial DNA (mtDNA) sequence analysis of Chinese Holstein cattle from Beijing (n = 41) and a comparison of them with the published mtDNA sequences (n = 293) of 14 Asian breeds with an emphasis on Chinese native cattle breeds. Three shared common haplotypes between Chinese Holstein cattle and native Asian cattle were found. Moreover, a high level of haplotype diversity in Chinese Holstein cattle (h = 0.9557) and low nucleotide diversity (${\pi}$ = 0.0052) was found, indicating a past population bottleneck followed by rapid population growth. These findings are supported by the significantly negative deviation of Tajima's D (-1.82085), the star-like pattern of dominant haplotypes and the pairwise mismatch distribution analysis, which showed a unimodal pattern.

• Korean Journal of Veterinary Service
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• v.42 no.2
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• pp.117-120
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• 2019

Bovine viral diarrhea Virus (BVDV) infections cause respiratory, gastrointestinal, and reproductive problems, such as infertility, abortion, stillbirth, and sickly offspring. Many countries have reduced the economic damage through the application of different control programmes, and some have successfully eradicated BVD. Detection and elimination of cattle persistently infected (PI) with BVDV is important for BVD eradication because PI cattle are a main source of BVD transmission. In this study, the prevalence of Korean native cattle persistently infected (PI) with BVDV was investigated and determined in 49 farms with 3,050 cattle. The all samples were collected by ear notch sampling. Korean native cattle with initial positives on antigen-ELISA (Ag-ELISA) were sampled again after 3~4 weeks and cattle with second positives in both Ag-ELISA and immunohistochemistry (IHC) were identified as PI cattle. Among the 49 farms, 14 (28.6%) farms had at least more than one PI cow and 21 (0.69%) of 3,050 cattle were determined as PI cattle. As a result of this work, it is suggested that national BVD eradication program is required to reduce economic losses by BVDV infection in Korean cattle industries.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.733-736
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• 2004

The study was carried out on eight Bos indicus cattle breeds namely, Sahiwal, Tharparkar, Nimari, Khilari, Deoni, Amritmahal, Hariana and Hilly cattle; two Bos taurus cattle breeds namely, Jersey and Holstein Friesian and Indicine${\times}$Taurine crossbred cattle to find out the polymorphic pattern of beta-lactoglobulin gene. The polymorphism at beta-lactoglobulin gene was detected by conducting PCR-RFLP studies on 398 bp fragment spanning over 104 bases of exon IV and 294 bases of intron IV. Two alleles A and B and three genotypes AA, AB and BB were observed in all the cattle breeds. The frequency of B allele was comparatively higher than that of A allele. The allelic frequency of A varied from 0.20 to 0.30 in Bos indicus cattle breeds and 0.19 to 0.34 in Bos taurus breeds while in crossbred cattle the frequency was estimated as 0.21. The weighted frequency of A allele was highest in Indian cattle and lowest in crossbred cattle while the frequency in taurine cattle was found to be in between indicus and crossbred cattle. The non-significant differences of allelic frequency amongst Bos indicus, Bos taurus and crossbred cattle was observed. The effect of genotype on fat percentage was also found to be non-significant in cattle.

• Korean Journal of Veterinary Service
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• v.32 no.3
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• pp.219-225
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• 2009

A cross sectional survey was conducted to determine the seroprevalence of brucellosis in cattle in Bangladesh Agricultural University (BAU) Veterinary Clinics, in BAU Dairy Farm and Vabokhali from June 2008 to November 2008. A total of 200 serum samples were collected from BAU Veterinary Clinic, from BAU Dairy Farm and Vabokhali. Among the serum samples 143 sera samples were collected from BAU Veterinary Clinic, 42 serum samples from BAU Dairy Farm and 15 serum samples from Vabokhali. Sera were separated from blood samples and tested with specific Brucella abortus antigen (BAA) test and B. melitensis antigen (BMA) test. The overall seroprevalence of brucellosis in cattle was 5% in BAA and 0.5% in BMA. It was observed that, a significant higher prevalence of B. abortus was found in female than male. An insignificant higher prevalence of brucellosis was found in adult cattle (aged above 5 years), in cross breed cattle, in cattle with grazing, cattle breed by natural breeding, and in pregnant cows. Although insignificant but a higher prevalence of brucellosis was found in aged cattle than young cattle, cross bred cattle, pregnant cattle than non pregnant cattle, cattle with grazing. A higher prevalence of brucellosis was found in female cattle than male.

• Food Science of Animal Resources
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• v.34 no.2
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• pp.214-220
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• 2014

We investigated nutritional characteristics and active components in the liver of Wagyu${\times}$Qinchuan cattle and Qinchuan cattle produced in Shaanxi (China). We observed significant differences (p<0.05) in the proximate composition of protein, fat, carbohydrate, total energy, and glycogen. Wagyu${\times}$Qinchuan cattle liver showed higher (p<0.05) sodium, iron, zinc, and selenium concentrations than Qinchuan cattle liver. The amino acid composition of Wagyu${\times}$Qinchuan cattle liver was richer (p<0.05) in 13 types of amino acids, with the exception of Asp (10.06%), Val (5.86%), and Met (1.72%). Total essential amino acids accounted for almost half the composition (39.69%) in Wagyu${\times}$Qinchuan cattle liver. Wagyu${\times}$Qinchuan cattle liver had lower (p<0.05) levels of monounsaturated fatty acids (18.2%), but higher (p<0.05) levels of polyunsaturated fatty acids (35.11%), compared with Qinchuan cattle liver (23.29% and 28.11%, respectively). The thrombogenic index was higher in Qinchuan cattle liver (0.86) than in Wagyu${\times}$Qinchuan cattle liver (0.70), and the glutathione (38.0 mg/100g) and L-carnitine (2.12 ${\mu}M/g$) content was higher (p<0.05) in Wagyu${\times}$Qinchuan cattle liver than in Qinchuan cattle liver (29.8 mg/100g and 1.41 ${\mu}M/g$, respectively). According to the results obtained, the liver of Wagyu${\times}$Qinchuan cattle, which is insufficiently used, should be increasingly utilized to improve its commercial value.

• Korean Journal of Veterinary Service
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• v.34 no.3
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• pp.251-258
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• 2011

This study was performed to determine the prevalence of Theileria sergenti (T. sergenti) in cattle reared in eastern areas of Gyeongbuk province by PCR. Among 443 samples collected from 42 cattle farms, 96 (21.7%) samples from 13 (31.0%) farms were positive for T. sergenti. By regions, 87 (26.6%) out of 327 cattle in Gyeongju, 8 (9.5%) out of 84 cattle in Pohang, and 1 (3.1%) out of 32 cattle in Ulleung were positive for T. sergenti. T. sergenti infection in dairy cattle (27.9%) was significantly higher than that in Korean cattle (9.4%, P<0.0001). Accordingly, Korean cattle were more resistant to T. sergenti infection. Prevalence of T. sergenti in cattle was increased with age (P<0.0001). The infection rate in cows (23.3%) was significantly higher than that in bulls (5.0%, P<0.01). Seasonally, prevalence of T. sergenti in cattle was highest in autumn (32.7%, P<0.01). Prevalence of T. sergenti in grazing cattle (66.7%) was significantly higher than that in non-grazing cattle (15.8%, P<0.0001). Since prevalence of T. sergenti infection is high in cattle reared in eastern areas of Gyeongbuk province, survey on other hemoparasites and appropriate control programs need to be established in this region.