• Title/Summary/Keyword: Cathepsin B inhibitor, S, misakiensis

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Identification of Streptomyces misakiensis Producing Cathepsin B Inhibitor and the Purification of Inhibitor (Cathepsin B 저해물질을 생산하는 Streptomyces misakinesis의 동정 및 저해물질의 분리)

  • 한길환;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.1
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    • pp.25-30
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    • 2001

  • A strain of Actinomycetes producing cathepis B inhibitor was isolated from soil and identified as Streptomyces misakiensis. The product of S. misakiensis inhibited effectively cathepsis B proteinases as well as trypsin and papain. The cathepsin B inhibitor were largely produced with incubation for 4 days. The S. misakiensis was the most growth with incubation for 5 days. The cathepsin B inhibitor was isolated from the extraction of both with ethanol, ethanol and chlorofrom, and following several column chromatography such as sephadex G-15, silica gel 60 and sephadex LH-20 chromatography. The moleculer weight of purfied inhibitor was 138 dalton.

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