• Asian-Australasian Journal of Animal Sciences
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• 제22권10호
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• pp.1437-1440
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• 2009

This study was performed to investigate the use of urinary catecholamines to monitor changes in the activity of the sympathetic nervous system and to determine the relationship of urinary cortisol and catecholamines in Japanese Shorthorn cows in response to social isolation. One cow was isolated from its group, which consisted of 14 cows (457 to 756 kg BW, 2 to 12 years old), for three days. The isolated cow was in contact with the other cows visually only at meal times. This isolation was repeated for 6 cows. Spontaneously voided urine samples were collected from the experimental animals once a day, before the treatment and on days 1, 2, and 3. Urinary cortisol and adrenaline levels were significantly increased compared with pre-isolation levels on the first day, and then declined to the basal levels during the next two days. Urinary noradrenaline levels changed in the same way as cortisol and adrenaline levels, but the difference was not significant. Urinary cortisol levels tended to be correlated with those of urinary adrenaline, but not noradrenaline. This study suggests that the urinary adrenaline levels can be a non-invasive indicator of stress and that the change of urinary adrenaline is similar to that of urinary cortisol.

• 동의신경정신과학회지
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• 제4권1호
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• pp.77-97
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• 1993

Human and animals are living by acclimation to environmental changes like high and cold temperature, nose, confinement, etc. If the above changes reach a defined levels, some physiological abnormal state could appear, which we call them as stress state. Catecholamines are excreted by the sympathetic-adrenomedullary system in free from in urine. Catecholamines are derived from the adrenal medulla and urinary epinephrine can be taken as a rough estemation of the activity of this gland. Many scientist reported the endocrinological change, excretion of catecholamine and its metabolites, stomach ulcer formation, etc. under the condition of the confinement and high temperature. In this study author gave restraint, electric shock and immersion stress to rats by administrating by HPLC and got the following results. 1. In the restriant experiment, epinephrine contents in control rat was 194.7 ng, but in Bohyulanshintang administered rat urine 198.9 ng of epinephrine was found. 2. In the electrical shock experiment, 199.5 ng of epinephrine was found in the control rat urine, but in Bohyulanshintang administered rat urine epinephrine content was 142.4 ng. 3. Dopamine contents in control rat urine the immersion environment was 118.9 ng, but in Bohyulanshintang administered rat urine only 55.2 ng of dopamine was found. 4. Incontrol rat stomach there appeared focal erosion and inflamatory exudate, but in experimental group these symptom were turned to mild condition.

• 정신신체의학
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• 제15권2호
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• pp.65-72
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• 2007

정신과에 내원하는 환자들 중에서 피로를 호소하는 환자들의 비율이 높으며, 만성적인 피로감을 호소하는 환자들에서 다른 우울증과 같은 정신과질환이 공존하는 경우도 많다. 환자들은 많은 괴로움을 호소하나, 기저의 임상상태의 종류와 심각도에 대해서는 비특이적이므로 실제로는 임상가에 의해서 무시되는 경우가 많으며, 그 중요성이 과소평가되는 경우가 많아서, 적절한 처치를 받지 못하고 삶의 질에도 많은 영향을 받게 된다. 피로는 말초와 중추신경계 둘 다에서 영향을 받는 다차원적인 현상으로 이해하여야 한다. 환자가 호소하는 피로증상에 대해서 좀 더 적극적으로 조절하기 위한 여러 약물학적 및 비약물학적 접근법들이 시도되고 있다.

• Journal of Acupuncture Research
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• 제20권3호
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• pp.177-193
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• 2003

Objective : The present studies investigated the effects of 120Hz high frequency electroacupunctue(EA) on the stress-induced stomach dysfunction in relation to its effect on the level of stress hormone and gastric mucosal damages. The gastric mucosal injury was induced by cold-restraint stress and two acupoints corresponding to Zusanli and Sanyinjiao in man were used. Methods: Cold-restraint stress produced typical gastric lesions in all rats of the stressed groups, but he number of ulcers as well as the mean ulcer diameter were reduced by 120 Hz EA pre-treatment. The cold-restraint stress also induced an increase in catecholamine response involving epinephrine, norepinephrine and dopamine, but an slight decline were observed in EA pre-treated rats compared with cold-restrained rats. Results: The degranulation value of gastric mast cell was significantly higher in cold-restrained rats than in control ones. However, with the significant reduction of degranulation values of gastric mast cells in EA pre-treated rats compared with cold-restrained ones, $PGE_2$ content in the gastric mucosa of EA pre-treated rats was also different from that observed in cold-restrained rats. Cold-restraint stress induced an elevated mRNA expression of pro-inflammatory gene such as cyclooxygenases-2 and tumor necrosis factor(TNF)-${\alpha}$, but these expression were down-regulated in EA pre-treated rats. Immunohistochemecal analysis showed that while the inhibitory-${\kappa}B{\alpha}$ an TNF-${\alpha}$ immunoreaction in the surface epithelium of the stomach tended to increase, both reactions in the EA pre-treated rats showed similar pattern as observed in controls. Conclusions : These results suggest that 120 Hz EA may act as a therapeutical means for gastric mucosal damages through a activation of pituitary adrenal system. it could be concluded that 120 hz high frequency electroacupuncture affords a good protective potential against stress-induce gastrointestinal dysfunction.

• Journal of Genetic Medicine
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• 제4권2호
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• pp.179-185
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• 2007

목 적:카테콜라민은 교감신경계에서 신경전달물질이며 스트레스자극에 의해 활성화된다. TH와 DBH는 카테콜라민 합성에 매우 중요한 효소이다. CRH는 스트레스 반응에서 방출되는 호르몬이다. 이번 연구의 목적은 부동스트레스가 흰쥐 뇌에서 TH, BDH와 CRH 유전자발현에 어떤 영향을 미치는가를 알아보기 위한 것이다. 방 법:2시간 동안 부동스트레스와 무처치 흰쥐의 뇌에서 TH, DBH와 CRH 유전자 발현량을 비교하였다. TH, DBH와 CRH 유전자 발현은 RT-PCR과 western blotting analysis에 의해 정량하였다. 결 과:부동스트레스 흰쥐 그룹의 뇌와 부신에서 TH와 DBH 유전자발현은 정상그룹보다도 약 2-3배 유의하게 증가하였으며, CRH유전자는 약 1.5배 유의하게 증가하였다. 결 론:이번 연구는 흰쥐의 뇌와 부신에서 TH, DBH와 CRH 유전자는 스트레스 자극에 의해 발현이 활성화됨을 확인할 수 있었다.

• 대한약리학회지
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• 제19권1호
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• pp.61-69
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• 1983

Hypothermia is an essential preparatory procedure for cardiac surgery, which lows the metabolic rate and myocardial oxygen demand. However, hypothermia itself is a stress enough to change the tonus of sympathoadrenal system, especially the cardiovascular responses to the catecholamines. It is reported that the positive chronotropic and inotropic response of catecholamines is exaggerated during hypothermia because of decreased norepinephrine uptake at the junctional cleft or decreased catecholamine metabolism. On the other hand, there are evidences of diminished catecholamines responses in low temperature ana further, interconversion of adrenergic receptors is also suggested. Present investigation was planned to observe the cardiovascular changes and its responses to catecholamines during surface hypothermia in cat. Healthy mongrel cats, weighing $2{\sim}3\;kg$, anesthetized with secobarbital(30 mg/kg), were permitted to hypothermia by external cooling technic. Esophageal temperature, ECG (lead II), heart rate, left ventricular pressure with dP/dt, carotid artery pressure and left ventricular contractile force were monitored with Polygragh (Model 7, Grass), and the respiration was maintained with artificial respirator (V 5 KG, Narco). Followings are summarized results. 1) Surface cooling caused progressive decrease of body temperature and reached $l8.8{\pm}0.8^{\circ}C$ and $16.9{\pm}0.6^{\circ}C$ in 120 and 150 min respectively, after immersion into ice water, and ventricular fibrillation was developed at $20.4{\pm}0.65^{\circ}C$. 2) Heart rate, blood pressure and myocardial contractility were decreased after initial increase as the body temperature falls. 3) Systolic and diastolicdd P/dt of left ventricular pressure were decreased and that the decrement of diastolic dP/dt was more marked. 4) On ECG, ST depression, Twave inversion and prolongation of PR interval were prominent in hypothermia, and moreover, the prolongation of PR interval was marked just prior to the development of ventricular fibrillation. 5) The cardiovascular responses to catecholamines, especially to isoproterenol, were suppressed under hypothermia.

• Journal of Yeungnam Medical Science
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• 제32권2호
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• pp.132-137
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• 2015

Adrenocorticotropic hormone (ACTH)-producing pheochromocytoma has been rarely reported, whereas only a few cases of Cushing syndrome accompanied by opportunistic infections have been reported. We experienced a patient with pheochromocytoma with ectopic Cushing syndrome complicated by invasive aspergillosis. A 35-year-old woman presented with typical Cushingoid features. Her basal plasma cortisol, ACTH, and 24-hour urine free cortisol levels were significantly high, and 24-hour urine metanephrine and catecholamine levels were slightly elevated. The endogeneous cortisol secretion was not suppressed by either low- or high-dose dexamethasone. Abdominal computed tomography (CT) revealed a heterogeneous enhancing mass measuring approximately 2.5 cm in size in the left adrenal gland. No definitive mass lesion was observed on sellar magnetic resonance imaging. On fluorine-18 fluorodeoxyglucose positron emission tomography/CT, a hypermetabolic nodule was observed in the left upper lung. Thus, we performed a percutaneous needle biopsy, which revealed inflammation, not malignancy. Thereafter, we performed a laparoscopic left adrenalectomy, and its pathologic finding was a pheochromocytoma with positive immunohistostaining for ACTH. After surgery, the biochemistry was normalized, but the clinical course was fatal despite intensive care because of the invasive aspergillosis that included the lungs, retina, and central nervous system.

• 대한본초학회지
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• 제30권3호
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• pp.69-76
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• 2015

Objectives : The effect of mixture extracted from Bupleuri Radix and Physalidis Herba(BR+RH) on the LPS-induced Depression in rats was investigated. Methods : Rats were administered intragastrically BR+PH after injectio of LPS to induce deprssion. Immobility was examined using Tail Suspension Test(TST), Forced Swimming Test(FST). The level of plasma corticosterone was measured by an Enzyme-Linked Immunosorbent Assay(ELISA) method. The expressions of c-Fos, Corticotropin Releasing Factor(CRF), NADPH-d in the Paraventricular nucleus(PVN) and TH in the Locus coeruleus(LC) were measured by immunohistochemical method. Results : In the effect of BR + PH on TST, immobility was significantly decreased comparing with the LPS group. In FST, immobility was shown decrease tendency in the BR+PH group. The expression of c-Fos in the PVN was significantly decreased at BR + PH400 group, comparing with the LPS group. The expression of CRF in PVN was shown dto have the decrease tendency in the BR+PH group, comparing with the LPS group. The expression of NADPH-d in PVN was not significantly decreased at BR+PH groups, comparing with the LPS group. The expression of TH in the LC was shown to have the decrease tendency at BR + RH groups, but not significantly, comparing with the LPS group. Conclusions : Anti-depressant effect of mixture after extracted from Bupleuri Radix and Physalidis Herba was through the anti-inflammatory effect via inhibition of HPA axis. NO and catecholamine system is not involved.

• 대한약침학회지
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• 제17권1호
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• pp.74-79
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• 2014

Objectives: This study was conducted to clarify the analgesic effect of toad cake and toad-cake-containing herbal drugs. Methods: We counted the writhing response of mice after the intraperitoneal administration of acetic acid as a nociceptive pain model and the withdrawal response after the plantar surface stimulation of the hind paw induced by partial sciatic nerve ligation of the mice as a neuropathic pain model to investigate the analgesic effect of toad cake and toad-cake-containing herbal drugs. A co-treatment study with serotonin biosynthesis inhibitory drug 4-chloro-DL-phenylalanine methyl ester hydrochloride (PCPA), the catecholamine biosynthesis inhibitory drug ${\alpha}$-methyl-DL-tyrosine methyl ester hydrochloride (AMPT) or the opioid receptor antagonist naloxone hydrochloride was also conducted. Results: Analgesic effects in a mouse model of nociceptive pain and neuropathic pain were shown by oral administration of toad cake and toad-cake-containing herbal drugs. The effects of toad cake and toad-cake-containing herbal drugs disappeared upon co-treatment with PCPA, but not with AMPT or naloxone in the nociceptive pain model; the analgesic effect of toad-cake-containing herbal drugs also disappeared upon co-treatment with PCPA in the neuropathic pain model. Conclusion: Toad cake and toad-cake-containing herbal drugs have potential for the treatments of nociceptive pain and of neuropathic pain, such as post-herpetic neuralgia, trigeminal neuralgia, diabetic neuralgia, and postoperative or posttraumatic pain, by activation of the central serotonin nervous system.

• 대한불안의학회지
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• 제4권1호
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• pp.19-27
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• 2008

Objective : This study was conducted to evaluate the changes in the levels of neurotransmitters and cortisol in patients with chronic posttraumatic stress disorder (PTSD) and to evaluate their correlation with symptoms after long-term pharmacological treatment. Methods : Twenty-eight Vietnam veterans with chronic PTSD and 34 non-PTSD patients were consecutively recruited. The Combat Exposure Scale (CES), Mississippi Scale for Combat-Related Posttraumatic Stress Disorder (M-PTSD), Clinician Administered PTSD Scale (CAPS), Hamilton Rating Scale for Depression (HRSD) and Hamilton Anxiety Scale (HAS) were used to evaluate symptom severity. High performance liquid chromatography (HPLC) was used to measure the plasma levels of epinephrine, norepinephrine, and dopamine, and a radioimmunoassay (RIA) was performed to evaluate the plasma level of cortisol. Results : Plasma cortisol was significantly lower in PTSD patients than in control subjects, while there was no significant difference in plasma epinephrine, norepinephrine and dopamine between the two groups. The scores of M-PTSD, CAPS, HAMD and HAMA were signigicantly higher in PTSD patients than control group. Conclusion : After long-term treatment, the levels of neurotransmitters in PTSD patients returned to within the normal range, and the patients' symptoms showed some improvement. However, the core symptoms of PTSD continued to appear intermittently, and they are thought to be associated with hormonal systems, such as the HPA axis. It is also suggested that PTSD should be considered to be a complex disorder associated with multiple systems and that combinations of the effective medications for each system should be used to treat patients with PTSD.