• 제목/요약/키워드: Catechol.

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Is catechol-o-methyltransferase gene polymorphism a risk factor in the development of premenstrual syndrome?

  • Deveci, Esma Ozturk;Incebiyik, Adnan;Selek, Salih;Camuzcuoglu, Aysun;Hilali, Nese Gul;Camuzcuoglu, Hakan;Erdal, Mehmet Emin;Vural, Mehmet
    • Clinical and Experimental Reproductive Medicine
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    • 제41권2호
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    • pp.62-67
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    • 2014

  • Objective: The objective of this study was to investigate whether there was a correlation between catechol-o-methyltransferase (COMT) gene polymorphism, which is believed to play a role in the etiology of psychotic disorders, and premenstrual syndrome (PMS). Methods: Fifty-three women with regular menstrual cycles, aged between 18 and 46 years and diagnosed with PMS according to the American Congress of Obstetrics and Gynecology criteria were included in this study as the study group, and 53 healthy women having no health problems were selected as the controls. Venous blood was collected from all patients included in the study and kept at $-18^{\circ}C$ prior to analysis. Results: There was no significant difference between the groups in terms of demographic features such as age, body mass index, number of pregnancies, parity, and number of children. No statistically significant difference was observed in terms of COMT gene polymorphism (p=0.61) between women in the PMS and the control groups. However, a significant difference was found between arthralgia, which is an indicator of PMS, and low-enzyme activity COMT gene (Met/Met) polymorphism (p=0.04). Conclusion: These results suggested that there was no significant relationship between PMS and COMT gene polymorphism. Since we could not find a direct correlation between the COMT gene polymorphism and PMS, further studies including alternative neurotransmitter pathways are needed to find an effective treatment for this disease.

  • https://doi.org/10.5653/cerm.2014.41.2.62 인용 PDF KSCI

The Modified Electrode by PEDOP with MWCNTs-Palladium Nanoparticles for the Determination of hydroquinone and Catechol

  • Naranchimeg, Orogzodmaa;Kim, Seul-Ki;Jeon, Seung-Won
    • Bulletin of the Korean Chemical Society
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    • 제32권8호
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    • pp.2771-2775
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    • 2011

  • Poly-ethylenedioxypyrrole (PEDOP) coated thiolated multiwall carbon nanotubes palladium nanoparticles (MWCNTs-Pd) modified glassy carbon electrode (GCE) [PEDOP/MWCNTs-Pd/GCE] for the determination of hydroquinone (HQ) and it’s isomer catechol (CA) were synthesized and compared with bare GCE and thiolated multiwall carbon nanotubes (MWCNTs-SH/GCE). The modification could be made by simple processes on a GCE with MWCNTs-Pd covered by PEDOP in a 0.05 M tetrabutylammonium perchlorate (TBAP)/MeCN solution system. A well-defined peak potential evaluation of the oxidation of hydroquinone to quinone at 0.05 V (vs. Ag/AgCl), and electrochemical reduction back to hydroquinone were found by cyclic voltammetry (CV) in phosphate buffered saline (PBS) at pH 7.4. Peak current values increased linearly with increasing hydroquinone contents. The peak separation between the anodic and cathodic peaks at the PEDOP/MWCNTs-Pd/GCE was ${\Delta}Ep$ = 40 mV for HQ and ${\Delta}Ep$ = 70 mV for CA, resulting in a higher electron transfer rate. Moreover, good reproducibility, excellent storage stability, a wide linear range (0.1 ${\mu}M$ - 5 mM for HQ and 0.01 ${\mu}M$ - 6 mM for CA), and low detection limits ($2.9{\times}10^{-8}$ M for HQ and $2.6{\times}10^{-8}$ M for CA; S/N = 3) were determined using differential pulse voltammetry (DPV) and amperometric responses; this makes it a promising candidate as a sensor for determination of HQ and CA.

  • https://doi.org/10.5012/bkcs.2011.32.8.2771 인용 PDF KSCI

A Challenging Study to Identify Target Proteins by a Proteomics Approach and Their Validation by Raising Polyclonal Antibody

  • Jeong, Da-Woon;Park, Beom-Young;Kim, Jin-Hyoung;Hwang, In-Ho
    • 한국축산식품학회지
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    • 제31권4호
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    • pp.506-512
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    • 2011

  • This study was conducted to validate the theoretical feasibility of a technique to identify biomarkers in Korean native black pig (KNP) and a commercial Landrace breed. Using two-dimensional electrophoresis, we found six proteins (NADH dehydrogenase Fe-S protein 1, an unnamed protein product, similar to T-complex protein 1, annexin V = CaBP33 isoform, fatty acid-binding protein, and catechol O-methyltransferase), which appeared in KNP alone. We raised polyclonal antibodies (used as the primary antibody) for Western blotting to confirm the characteristics of the six KNP proteins. As a result, catechol O-methyltransferase, annexin V = CaBP33 isoform, and the unnamed protein product presented thicker bands in KNP than those in Landrace. Moreover, catechol O-methyltransferase was shown to be more feasible as a biomarker for KNP. However, cross-reactivity was observed with the polyclonal antibodies for KNP and the other three proteins (NADH dehydrogenase, a protein similar to T-complex protein 1, and fatty acid-binding protein). This study only showed limited results from a limited number of animals; however, our research suggests possibilities for future studies.

  • https://doi.org/10.5851/kosfa.2011.31.4.506 인용 PDF KSCI