• 한국임상수의학회지
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• 제16권2호
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• pp.409-412
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• 1999

To establish the electroacupuncture anesthesia for pigs, 6 piglets (Landrace mixed, 1 month old, 3.7~5.3 kg, female: 3 heads and male: 3 heads) were examined. The acupoints used for porcine electroacupuncture anesthesia were An-shen, and Tian-ping and Bai-hui. After horizontal insertion of needle to left and right An-shen, and perpendicular insertion to Tian-ping and Bai-hui, respectively, positive electrode was connected at right An-shen and Tian-ping, and negative electrode was connected at left An-shen and Bai-hui using veterinary electroacupuncture anesthesia apparatus, respectively. Electric condition was 3 V and 30 Hz. To examine the effect of electroacupuncture anesthesia, laparotomy (4 heads) and castration (2 heads)were applied. The induction time of electroacupuncture anesthesia was very rapid and about 1 minute, and pain of the body surface including the extremities was not detected. As for the reactions with electroacupuncture anesthesia, the head was directed to backward, nasal part was curved to one side, and blepharum was closed in part of changed nasal part. In addition, consciousness was vivid, and muscle tension and lift of the tail were observed in early stage but was relaxated afterwards. The pain was not found during incision and suture, bleeding was comparatively small volume and the class of anesthesia effect was excellent.

• Asian Pacific Journal of Cancer Prevention
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• 제16권12호
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• pp.4909-4913
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• 2015

Objective: To explore the preventive effect of aspirin on the cardiovascular complications in prostate cancer after endocrinotherapy. Materials and Methods: A total of 92 patients with prostate cancer were divided into observation group (n=44) and control group (n=48). The control group was treated with medical castration plus anti-androgenic drugs. Based on the above treatment, the observation group was added aspirin. The follow-up duration was 2 years. The changes of partial prothrombin time (PT), activated partial thromboplastin time (APTT), platelet aggregation rate (PAG), prostate-specific antigen (PSA) and serum testosterone (T) before and after treatment as well as incidence of cardiovascular disease were observed. Results: The 2-year survival rates of patients without cardiovascular disease in observation group and control group were 95.45% (42/44) and 72.92% (35/48), respectively, and significant difference was presented between two groups by comparison to the survival rates ($x^2=8.5453$, p=0.0035). There was no statistical significance between two groups as well as before and after treatment regarding PT (p>0.05). After treatment, APTT went down and PAG was gradually on the rise in control group, while PAG down and APTT on the rise increasingly in observation group. Significant differences were presented between two groups as well as before and after treatment (p<0.01). Both PSA and T levels were decreased significantly in two groups after treatment (p<0.01), but there was no statistical significant between two groups (p>0.05). Conclusions: Application of endocrinotherapy in prostate cancer can easily lead to occurrence of cardiovascular disease, but cardiovascular complications can be prevented by aspirin, without affecting the effect of endocrinotherapy.

• 한국수정란이식학회지
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• 제27권2호
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• pp.101-105
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• 2012

Linuron is a pesticide with a weak anti-androgenic property, which impacts male reproductive organs. In this study, to clarify whether linuron affects the cellular antioxidant system of ventral prostate, gene expression patterns of the representative antioxidant enzymes such as glutathione peroxidase (GPx), selenoprotein P (SePP), and superoxide dismutase (SOD) were investigated in the rat ventral prostates exposed to linuron using real-time RT-PCR analyses. Sprague-Dawley rats castrated at 6 weeks old were treated with linuron (25, 50, or 100 mg/kg per oral) daily for 10 days after testosterone propionate administration (0.4 mg/kg) subcutaneously. As compared to normal control animals, mRNA levels of phospholipid hydroperoxide GPx (PHGPx), SePP, and Mn SOD significantly increased in the prostates exposed to linuron (25, 50, and 100 mg/kg). However, cytosolic GPx (100 mg/kg) and Cu/Zn SOD (25, 50, and 100 mg/kg) mRNA levels significantly decreased in the ventral prostates. These results indicate that linuron upregulates the expressions of PHGPx, SePP, and Mn SOD mRNAs, but down-regulates the expressions of cytosolic GPx and Cu/Zn SOD in rat prostates, suggesting that linuron may have dual effects in the cellular antioxidant system of prostate.

• 철학연구
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• 제115권
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• pp.249-279
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• 2010

헤겔의 『정신현상학』 '이성' 장에 등장하는 '마음의 법칙'은 이성의 자기 확신이 '광기'의 또 다른 모습이 될 수 있는가를 보여준다. 이성의 의심할 수 없는 확신(Gewissheit)은 데카르트에게는 진리의 징표이고, 칸트에게는 양심(Gewisse)의 도덕률이다. 하지만 이러한 주관적 확신은 의식과 현실의 차이를 무시하고 현실을 의식과 일치시키려는 자만의 광기에 빠질 수 있다. 동키호테식의 이상론자나 낭만주의적 개혁논자들에게서 전형적으로 보이는 이러한 태도는 근대적 이성과 '정신병'이 동전의 양면이 될 수 있음을 나타낸다. 라캉의 '거울단계'의 이론, 상상 계 이론, 욕망의 공식 등은 자아의 완전성과 통일성의 이미지가 오해의 산물임을 보여준다. 이 단계는 주체의 형성 과정에서 필수적이기는 하지만 동시에 타자성의 영역인 언어와 법의 영역, 곧 상징계로 이행해야 한다. 자아는 아버지의 이름에 의해 행해지는 상징적 거세를 거치지 못할 경우 상상 계의 감옥에 갇혀 정신병에 빠질 수 있다. 헤겔의 '마음의 법칙'이 겪는 광기나 착란 등도 비슷한 경험의 과정을 보여준다. 헤겔의 경우 '아버지의 이름'은 불가피하게 욕망을 유예시키는 노동의 기율이나 혹은 절대 타자로서의 죽음이 그 역할을 대신한다. 이것은 자연적 존재의 개별자가 보편화되는 과정에서 겪는 분리와 지양의 경험, 곧 라캉식의 상징적 거세의 경험이라 할 수도 있을 것이다. 다만 헤겔의 경우 분리의 경험이 정신의 자발성에 기초한다면, 라캉의 경우는 절대 타자에 의해 강요되고 구조화되는 차이가 있을 것이다.

• 한국의류학회지
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• 제28권11호
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• pp.1395-1403
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• 2004

Exposure and Concealment have been continued as important way to express fashion and theme as well, especially in women fashion. Exposure can make themselves attractive, and concealment emphasizes this way of being attractive by exposure. Besides the temptable effect of exposure, the ideology which put women next men in society has made a prominence on the fact exposure is significant theme in women fashion. It means that women's image of 'Being seen as Object' (this can be explained by relation passive between active) in society has driven female emphasize in using and focusing on exposure to be attractive. Also the other important thing in women fashion has been considered is concealment. The fact that society has demand women more chaste duty in detail may have meaningful thing in the condition that instigates women take off being attractive to the subject, considering ideology, men. Therefore attractive femininity has to be suggested by virtue and temptation, it is common sense that looking just shallow or too strict are far from criteria of ideal beauty. Namely, the concept for exposure coexists with concealment not only constructively but also naturally in fashion. Technique that can be accentuate for effective exposure differently from method to show unilaterally is needed, and this technique can promote new fashion. And according to the theory of Freud, women means' Absence (lacking penis)', this female's Lack Image induces male castration complex therefore 'Fetishistic Look' which transfers something phallic into specific things or parts of body is adopted, sort of object cathexis. This is revealed as some parts of women body are diverted into object for sexual love, symbolic equivalent. For example, women's parts like legs or breast are remarked as erotogenic zone and as time goes, when no more the zone are felt sexual by exposure, other somewhere felt mysterious (felt something sexual) used to be stressed by exposing. As it were, it can be possible that exposing part on women body (sexual part) moved as time passed because of what previously stated. Especially, the concept of exposure and concealment in women fashion should explain not only from a point of view of style for the beautifulness but also from wearer's immanent intention, further more relationship with ideology. About the present situation becoming bolder in women fashion, it is said that women express confidence as the position of women is elevated higher socially, the more women get freedom. But if we consider this psychoanalysis opinion mentioned ahead, the possibility can not be excluded that the exposure can get emphasizing according as the need of feminine modesty gets decreasing from ideal femininity society, men want. This study about exposure and concealment was made progressed through the theory of Freud who originated psychoanalysis, presented many theories. But nowadays contradictions are being brought up against Freud's theory including conventional philosophy like male chauvinism and adjust studies get suggested. Hereupon, there may be limitation in making no allowance of contradiction and following his theory.

• 한국수정란이식학회지
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• 제28권3호
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• pp.297-302
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• 2013

Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of genetically elite populations. Castration, catastrophic injury, sudden death or any other event that makes semen collection or mating impossible may prematurely terminate a stallion reproduction. Stallion epididymal spermatozoa vary widely in the loss of progressive motility, acrosomal integrity, and viability during freezing and thawing. The objective of this work was to investigate the effect of (1) freezing package types on cryopreservation efficiency, (2) thawing temperatures (37, 56 or $70^{\circ}C$) on Computer Assisted Sperm Analysis (CASA) parameters and (3) post-thawing incubation time (0, 1, 2 or 4h) on castrated stallion epididymis. Post-thawed sperm motility ranged between 59.69% and 64.28% ($56^{\circ}C$ and $37^{\circ}C$) in various thawing temperatures. When stallion epididymis sperm was frozen, straw was better than freezing tube on VCL (Velocity of Curvilinear Line) and VAP (Velocity of Average Path) parameter. Higher percentage of motility was observed at $37^{\circ}C$ thawing temperature even though no significant difference was observed among various temperatures. The motility, VCL, ALH (Amplitude of Lateral Head displacement), VAP, BCF (Beat-Cross Frequency) and STR (Straightness index) parameter of post-thawed sperm were significantly decreased by increasing the incubation time for all thawing temperatures. The present study showed that type of freezing package (Straw vs. Freezing tube) was not significantly different on cryopreservation efficiency. Furthermore, stallion epididymal spermatozoa frozen-thawed at $37^{\circ}C$ for 1 min resulted the highest proportion of motility and velocity movement. In addition, motility and viability of frozen-thawed stallion epididymal spermatozoa were also decreased by incubation.

• 한국수정란이식학회지
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• 제26권4호
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• pp.265-270
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• 2011

Procymidone is a fungicide with anti-androgenic properties widely used to protect fruits from fungal infection, which induces an excessive reactive oxygen species production in male reproductive organs. In this study, to clarify whether procymidone affect the cellular antioxidant system of prostate at onset of puberty, gene expression patterns of the representative antioxidant enzymes such as cytoplasmic glutathione peroxidase (GPx1), phospholipid hydroperoxide GPx (PHGPx), selenoprotein P (SePP), cytoplasmic copper/zinc superoxide dismutase (SOD1), and manganese SOD (SOD2) were investigated in the rat ventral prostates exposed to procymidone using real-time RT-PCR analyses. Seven-week-old Sprague-Dawley rats castrated at 6 weeks old were treated with procymidone (25, 50, or 100 mg/kg per day) orally for 7 consecutive days after testosterone propionate (0.4 mg/kg per day) administration by subcutaneous injection. As compared to normal control animals, GPx1 mRNA expression in prostates significantly increased by the administration with TP and/or procymidone. However, PHGPx and SOD1 mRNA levels significanatly decreased by over 25 mg/kg of procymidone treatment and SePP and SOD2 mRNA levels was significanatly reduced by over 50 mg/kg of procymidone treatment. These findings indicate that procymidone may affect the antioxidant system of prostatic cells in up-regulation mode of GPx1, but in down-regulation modes of PHGPx, SePP, SOD1, and SOD2, suggesting that procymidone may affect differently the cellular antioxidant system of prostate according to the exposure doses.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1285-1290
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• 2014

The aim was to determine whether ultrasound targeted microbubble destruction (UTMD) promotes dual targeting of HSP72 and HSC70 for therapy of castration-resistant prostate cancer (CRPC), to improve the specific and efficient delivery of siRNA, to induce tumor cell specific apoptosis, and to find new therapeutic targets specific of CRPC.VCaP cells were transfected with siRNA oligonucleotides. HSP70, HSP90 and cleaved caspase-3 expression were determined by real-time quantitative polymerase chain reaction and Western blotting. Apoptosis and transfection efficiency were assessed by flow cytometry. Cell viability assays were used to evaluate safety. We found HSP72, HSC70 and HSP90 expression to be absent or weak in normal prostate epithelial cells (RWPE-1), but uniformly strong in prostate cancerous cells (VCaP). UTMD combined with dual targeting of HSP72 and HSC70 siRNA improve the efficiency of transfection, cell uptake of siRNA, downregulation of HSP70 and HSP90 expression in VCaP cells at the mRNA and protein level, and induction of extensive tumor-specific apoptosis. Cell counting kit-8 assays showed decreased cellular viability in the HSP72/HSC70-siRNA silenced group. These results suggest that the combination of UTMD with dual targeting HSP70 therapy for PCa may be most efficacious, providng a novel, reliable, non-invasive, safe targeted approach to improve the specific and efficient delivery of siRNA, and achieve maximal effects.

• 생명과학회지
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• 제29권8호
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• pp.904-915
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• 2019

전립선암은 전이성 종양 중의 하나로 치료를 위해 호르몬 요법이나 외과 적 거세 방법이 주로 수행되지만 많은 부작용을 나타내었다. 최근 많은 연구자들이 이러한 상황을 해결하기 위해 종양 미세 환경을 연구하고 있으며 그 중 면역 세포, 특히 대식세포는 종양 미세 환경의 중요한 구성요소이다. 정상적인 조건에서 대식세포는 여러 암세포에 대해 약한 종양 살균 활성을 갖으나 $interferon-{\gamma}$ 또는 lipopolysaccharide에 의해 활성화되면, 염증성 사이토카인 및 케모카인을 분비함으로써 암세포를 직접 또는 간접적으로 사멸 시키게 된다. 본 연구에서는, 마우스 대식세포인 RAW 264.7 세포에 Phellinus linteus 추출물을 처리하여 산화질소의 방출과 pro-inflammatory cytokine들을 real-time PCR과 ELISA 방법으로 분석하였다. RAW 264.7의 조정 배지는 48시간 동안 전립선 암세포처리하여 상피간엽세포전이 관련 유전자의 발현을 측정 하였다. 그 때에 mesenchymal 관련 유전자들인 N-cadherin, snail, twist, slug 및 cadherin 11이 감소했을 뿐만 아니라 epithelial 관련 유전자인 E-cadherin은 증가하였다. 또한 암 전이 및 신생 혈관 형성에 관여하는 vimentin, ccl2 및 vegfa가 감소되었는데, 이는 EMT가 암세포의 이동과 침범에 밀접한 관련이 있기 때문이다. 따라서 Phellinus linteu에 의해 자극된 RAW 264.7 세포의 조정 배지는 인간 전립선 암세포주인 PC-3 세포의 이동과 전이를 억제하고 EMT 경로를 조절한다는 것을 나타낸다.