• Journal of the Korean Solar Energy Society
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• v.32 no.6
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• pp.141-148
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• 2012

Ceiling cassette type air conditioner has been a main stream as a heating/cooling system recently in school, Korea. In this study, indoor thermal environments made by ceiling cassette type air conditioner were investigated by CFD simulation. Concentrations of $CO_2$ were investigated by a field measurement. Indoor thermal environment with the velocity inlet angle of $45^{\circ}$ from the ceiling in heating season was very ununiform so that thermal area was divided into two parts those the one is window side which is cold, and the other is corridor side which is hot. In cooling season under the same condition, there are areas too hot or too cold. If the velocity inlet angle is set in $30^{\circ}$ from the ceiling, indoor thermal environments was improved greatly in cooling season and heating season, too. Also, from the field measurement of $CO_2$ concentrations, it was suggested to install ventilators with proper air volume considered the number of class students.

• Parasites, Hosts and Diseases
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• v.53 no.3
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• pp.335-339
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• 2015

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of $Ca^{2+}$, $Mg^{2+}$, $K^+$, and $HCO_3{^-}$ in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.1008-1013
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• 2014

The use of antimicrobial agents for additives or therapeutics is strongly associated with a prevalence of antimicrobial resistance in commensal Enterobacteriaceae. We aimed to characterize integrons in Enterobacteriaceae isolates obtained from chicken cecums in Korea. Moreover, the correlation between integron gene cassettes and antimicrobial resistance was also investigated. A total of 90 isolates the belonged to Enterobacteriaceae were recovered from chickens grown at Gyeongsang and Chungcheong provinces in Korea. Antimicrobial susceptibility tests were performed by the disk diffusion method. PCR and DNA sequencing were also performed to characterize the gene cassette arrays of the integrons. Of the 90 Enterobacteriaceae isolates tested, 39 (43.3%) and 10 (11.1%) isolates carried class 1 and 2 integrons, respectively. Whereas the class 2 integron did not contain gene cassettes, the class 1 integrons carried seven different gene cassette arrays. The class 1 integrons harbored genes encoding resistant determinants to aminoglycosides (aadA1, aadA2, and aadA5), trimethoprim (dfrA1, dfrA12, dfrA17, and dfrA32), lincosamides (linF), and erythromycin (ereA). Moreover, the presence of a class 1 integron was significantly related to a high resistance rate of antimicrobial agents, such as spectinomycin and trimethoprim. We confirmed that diverse class 1 integrons were widely distributed in Enterobacteriaceae isolates from chickens and directly contributed to the resistance to diverse antimicrobial agents in Korea.

• Journal of the Korean Institute of Telematics and Electronics
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• v.12 no.3
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• pp.1-7
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• 1975

In this paper, a method of writing and reproducing high density data with ordinary Audio cassette deck is discribed. In writing, the data N or NRZ code are modulated into PM code to take the positive code N(1) and the negative code N(0) are taken from the complement of the NRZ code, each of which are written into 2 channel track. In reading, the error corrected and the clock pulse can be generated from the reading pulse itself. Also, without modifying the interior circuit of the deck it is possible to use the deck in both the data and audio by adapting a few simple circuits. Over the range of 25HZ-4KHZ, it was possible to write and reproduce at the speed of 787 bps transmission rate.

• Proceedings of the SAREK Conference
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• 2006.06a
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• pp.1180-1185
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• 2006

Modern people spend most of time at indoor space, such as office or classroom. Especially, occupants are exposed to the airtight indoor air quality (IAQ) for a long time, At present, many studies on the air-conditioning systems are more focused on the individual thermal comfort than the thermal efficiency due to increase of the concern of health. There are several factors which are influenced thermal comfort, such as temperature, humidity, convection and air movement, etc. Also, the individual factor, such as age, gender, Physical constitution and habit, should be considered. The 4-way cassette type air conditioner is known to bring out better performance about thermal comfort than the traditional one. This study is performed on the higher ceiling environment than the common buildings or classrooms. Also, this study analyzed on the Indoor thermal comfort by diffusing direction of 4-way cassette air conditioner with various discharge angles, $45^{\circ},\;50^{\circ},\;55^{\circ}$ and $60^{\circ}$. Using a commercial code, FLUENT, three-dimensional transient air thermal flow fields are calculated with appropriate wall boundary conditions and standard $k-{\epsilon}$ turbulence model. Results of velocity and temperature distributions are graphically depicted with various discharge angles.

• Journal of Radiation Industry
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• v.11 no.1
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• pp.1-6
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• 2017

Reported some level of bacteria in areas that are well made contact in Radiology imaging room evaluate the importance of cleanliness in the hospital management of equipment to check for the presence of pathogenic bacteria. Gwang-ju and Jeol-la city and medium-sized hospitals in the material with a cotton swab and rub evenly Radiology selection cassette, a handle, Apron of the imaging apparatus having the most contact with patients from July 2016 to August 2016 as a target in place and special studios 6, and saline solution will placed in a test tube containing. The swab sample was diluted 1,000 times, you can see the bacteria and the intestinal bacterial selective medium Trypticase Soy Agar (TSA), Muller-Hinton Agar (MHA), Eosin-Methylene Blue (EMB), ENDO(BD, NJ, USA) then incubated smear to. In the incubator (incubator, SANYO, Japan) was observed after incubation of bacteria and counting the total number of bacteria also Colonies (colony) suspected intestinal bacteria were isolated and cultured on KIA medium (BD, NJ, USA). As a result, it was found that this came Gram positive Coccus A hospital handle the F hospital, from the C Gram positive Coccus cassette and handle the F hospital. The striking yellow coloring Staphylococcus aureus 110 agar (STA 110) in the medium sample, but it is suspected staphylococcal Coccus to the final identification in the laboratory is not a single specimen of the two samples from Gram positive Coccus biochemical identification Identification Kit is an API could not, it was thought to be non-Staphylococcus aureus was cultured on blood agar suggesting that (BAP) blood of dance. Dynamic tests were conducted biochemical API kit of the two samples were identified from Gram positive Coccus bacteria Escherichia coli (E. coli) is F hospital cassette was confirmed Eenterobacter cloaca in A hospital possession. Did not aggregate O-26, O-111, O-157 and the serum test was conducted in the laboratory from the E. coli F cassette hospital.

• Journal of Life Science
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• v.28 no.11
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• pp.1277-1284
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• 2018

In this study, a repeated yeast integrative plasmid (R-YIp) harboring Cre/loxP system was constructed to integrate various gene expression cassettes into the yeast chromosome. The R-YIp system contains a reusable selective marker (CgTRP1), loxP sequence, and target sequence for integration. Therefore, many gene expression cassettes can be integrated into the same position of the same yeast chromosome. In the present study, several model enzymes involving xylan/xylose metabolism were examined, including endoxylanase (XYLP), ${\beta}$-xylosidase (XYLB), xylose reductase (GRE3) and xylitol dehydrogenase (XYL2). Efficient expression of these genes was obtained using two promoters (GAL10p and ADH1p) and various plasmids (pGMF-GENE and pAMF-GENE plasmids) were constructed. The XYLP, XYLB, GRE3, and XYL2 genes were efficiently expressed under the control of the GAL10 promoter. Subsequently, R-YIps containing the GAL10p-GENE-GAL7t cassette were constructed, resulting in pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids. These plasmids were sequentially integrated into chromosome VII of a Saccharomyces cerevisiae strain by repeated gene integration and selective marker rescue. These genes were integrated by the R-YIp system and were stably expressed in the yeast transformants to produce active recombinant enzymes. Therefore, we expect that the R-YIp system will be able to overcome current limitations of the host cells and allow selective marker selection for the integration of various genes into the yeast chromosome.

• The monthly packaging world
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• s.107
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• pp.137-145
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• 2002

• Proceedings of the Korean Society of Radiological Science
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• 1993.05a
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• pp.18-19
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• 1993

• Proceedings of the Korean Society of Radiological Science
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• 1993.05a
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• pp.19-20
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• 1993