• Journal of the Korean Applied Science and Technology
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• 제33권3호
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• pp.560-567
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• 2016

Acid-activated clays (SUPER-DC, DC-A3, and P1) are used for the bleaching of cold-pressed rapeseed oil. In this study we tested the bleaching performance of cold-pressed rapeseed oil according to the different reaction time (20, 40, 60, 80 min) and temperature (40, 80, $120^{\circ}C$). Oil color (lightness, redness, yellowness), pigments (chlorophyll A and carotenoid content) and quality properties (fatty acid composition, tocopherols (${\alpha}$, ${\beta}$, ${\gamma}$, ${\delta}$), and plant sterols (${\beta}$-sitosterol, campesterol, stigmasterol) content) were analyzed. The results showed that bleaching of cold-pressed rapeseed oil with 2% acid-activated clays at $40^{\circ}C$ for 20 min, brightness (L) increased, but redness (a) and yellowness (b) decreased. Bleaching of cold-pressed rapeseed oil with 2% DC-SUPER at $40^{\circ}C$ removed chlorophyll A and carotenoids pigments significantly. In addition, about 50% of total tocopherol content in cold-pressed rapeseed oil was reduced by bleaching. Originally total tocopherol content was 46.62mg/100g in cold-pressed rapeseed oil. But after bleaching, total tocopherol content was 12.67mg/100g (20 min bleaching), 15.31mg/100g (40 min bleaching), and 13.56mg/100g (60 min bleaching). However plant sterols content in cold-pressed rapeseed oil remained unchanged by bleaching. Overall, acid-activated clays were useful for the bleaching of pigmented rapeseed oil.

• Journal of Korean Society of Forest Science
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• 제99권5호
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• pp.711-719
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• 2010

We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM $CdSO_4$ solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6 mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6 mM Cd, but dry yield of P. thunbergii treated with 0.3 mM Cd was less affected and it was comparable with the control seedling. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3 mM Cd, which could be ascribed to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3 mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg $kg^{-1}$) without showing growth reduction, hence it might be used for phytoremediation of Cd contaminated areas.

• Korean Journal of Medicinal Crop Science
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• 제24권3호
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• pp.214-221
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• 2016

Background: This study was conducted to investigate the effects of germanium treatment on the growth and organic germanium production in the roots of Oplopanax elatus plantlets. Methods and Results: O. elatus plantlets were cultured in Murashige and Skoog (MS) medium with different concentrations of germanium dioxide ($GeO_2$) to analyze optimum growth conditions. Exogenous treatment of $10mg/{\ell}\;GeO_2$ promoted growth and an increase in the contents of chlorophyll a, b and carotenoid in O. elatus. The germanium accumulation and production in roots of O. elatus plantlets treated with organic germanium reached the highest levels. The growth of the aerial and underground portion of O. elatus with organic germanium was greater than that of the control. The accumulation and production of organic germanium reached the highest level ($40.89{\mu}g/plantlet$) with the treatment of $50mg/{\ell}\;GeO_2$. Antioxidant activity measured by DPPH and ABTS assays also increased with the germanium treatment and improved the DPPH and ABTS radical activity by 200% compared with that in the control. In addition, the total phenol and flavonoid contents of the plantlets with a treatment of $50mg/{\ell}\;GeO_2$ were higher than in the control. Conclusions: Taken together, the growth of O. elatus was increased with the treatment of $50mg/{\ell}\;GeO_2$ germanium and the biological references improved, with increased antioxidant activity and organic germanium production.

• FLOWER RESEARCH JOURNAL
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• 제16권2호
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• pp.134-142
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• 2008

This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

• Journal of the Korean Society of Marine Environment & Safety
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• 제27권2호
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• pp.340-354
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• 2021

The aim of this study was to investigate the spatial and temporal distribution of phytoplankton biomass and community composition in Jinhae Bay on the southern coast of Korea. Phytoplankton pigment analysis was conducted using ultra performance liquid chromatography (UPLC) were conducted from April to December 2019 at seven stations. Temperature, salinity, and dissolved oxygen (DO) and inorganic nutrients (dissolved nitrogen, dissolved phosphorus, and orthosilicic acid) were measured to investigate the environmental factors associated with the structure of phytoplankton community. Phytoplankton biomass (Chl-a) was the highest in July (mean 15.4±4.3 ㎍/L) and the lowest in December (mean 3.5±0.6 ㎍/L). Fucoxanthin was the most abundant carotenoid and showed a similar variation pattern to Chl-a, peridinin, and Chl-b. Phytoplankton community composition analysis showed that diatoms were a predominant group with an average abundance of 70 % whereas chlorophytes, cryptophytes, and dinoflagellates often appeared with lower averages. Further, the dominance of diatoms was closely correlated with water temperature and N:P ratio, which might be influenced by high temperatures in the summer and nutrient loading from the land. Additionally, freshwater and nutrient input by rainfall was estimated to be the most important environmental factor. Hence, the spatial and temporal variations in the composition of phytoplankton pigments and phytoplankton community were correlated with physicochemical and environmental parameters.

• Current Research on Agriculture and Life Sciences
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• 제5권
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• pp.89-99
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• 1987

The changes of lipid components in apple peels which effects physiological characteristic in apple storage, and pigment which is important base in quality, were investigated in terms of temperature and pressure. The results obtained were as follows ; The contents of total lipid of Ralls which is resistant to cold temperature were higher than those of Fuji. The contents of total lipid of Fuji and Ralls were decreased up to about 60 days and then increased up to the final stage of storage. Especially, the increasing rate at low temperature storage was greater than at normal temperature storage. In the case of Fuji, the contents of neutral lipid of normal temperature were increased up to 90 days, while those of low temperature were decreased up to 60 days and then increased up to 90 days. The contents of glycolipid, in both Fuji and Ralls were decreased up to about 30 days and then increased up to about 60 days and the decreased up to 90 days. The contents of phospholipid in Fuji were decreased during storage, while those in Ralls were appeared very slight changes. The ratios of unsaturated fatty acid to saturated fatty acids in neutral lipid of Fuji were decreased during storage, but those of Ralls were increased. Those of glycolipid of Fuji were decreased up to 60 days and then increased up to 90 days, while those in Ralls were increased during storage. In both Fuji and Ralls, those of phospholipid increased during storage and were high in order of sub-atmospheric pressure at $1^{\circ}C$ (SAP-L), normal atmospheric pressure at $1^{\circ}C$ (NAP-L), sub-atmospheric pressure at $20^{\circ}C$ (SAP-N) and normal atmospheric pressure at $20^{\circ}C$(NAP-N) for storage condition. The contents of chlorophyll of Ralls were higher than those of Fuji and the decreasing rate was the lowest in SAP-L. The changes of carotenoid and anthocyanin contents were the greatest increasing at NAP-N and colouring was delayed at SAP-L.

• Journal of the Society of Cosmetic Scientists of Korea
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• 제41권4호
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• pp.341-350
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• 2015

Skin carrys out protective role against harmful outer environment assaults including ultraviolet radiation, heavy metals and oxides. Especially, ultraviolet-B (UVB) light causes inflammatory reactions in skin such as sun burn and erythma and stimulates melanin pigmentation. Furthermore, the influx of UVB into skin cells causes DNA damage in keratinocytes and dermal fibroblasts, inhibition of extracellular matrix (ECM) synthesis which leads to a decrease in elasticity of skin and wrinkle formation. It also damages dermal connective tissue and disrupts the skin barrier function. Prolonged exposure of human skin to UVB light is well known to trigger severe skin lesions such as cell death and carcinogenesis. Haloarcula vallismortis is a halophilic microorganism isolated from the Dead Sea, Its growth characteristics have not been studied in detail yet. It generally grows at salinity more than 10%, but the actual growth salinity usually ranges between 20 to 25%. Because H. vallismortis is found mainly in saltern or salt lakes, there could exist defense mechanisms against strong sunlight. One of them is generation of additional ATP using halorhodopsin which absorbs photons and produces energy by potential difference formed by opening the chloride ion channel. It often shows a color of pink or red because of their high content of carotenoid pigments and it is considered to act as a defense mechanism against intense UV irradiation. In this study, the anti-inflammatory effect of the halophilic microorganism, H. vallismortis, extract was investigated. It was found that H. vallismortis extract had protective effect on DNA damage induced by UV irradiation. These results suggest that the extract of halophilic bacterium, H. vallismortis could be used as a bio-sunscreen or natural sunscreen which ameliorate the harmful effects of UV light with its anti-inflammatory and DNA protective properties.

• Journal of Korean Society of Forest Science
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• 제100권4호
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• pp.609-615
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• 2011

Forsythia saxatilis is a Korean endemic plant designated as rare and endangered by the Korea Forest Service (KFS). Growth and physiological characteristics of F. saxatilis were investigated under two different light intensities in order to figure out an appropriate growth environment for conservation and restoration of the species in its natural habitat. Shoot length, leaf size and weight, photosynthetic pigment content and photosynthetic parameters were measured for F. saxatilis grown at two experimental plots under relative light intensities (RLI) of 20% and 60% of the full sun, respectively. Fresh leaf weight of plants grown under high relative light intensities (RLI-60) exceeded that of plants grown at 20% RLI. The ratio of fresh leaf weight to leaf size at RLI-60 was 1.47 times superior comparing to that recorded at RLI-20. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were higher in plants grown at RLI-60, whereas the ratio of total chlorophyll to carotenoid content was higher in the leaves at RLI-20. Photosynthetic rate, stomatal conductance and transpiration rate at RLI-60 were, respectively, 2.5, 2.65 and 1.79 times higher comparing to those recorded at RLI-20. Water use efficiency, however, was higher at RLI-20. The chlorophyll/nitrogen ratio was 1.83 times higher at RLI-20 than at RLI-60. In contrast, the ratio of net photosynthesis to chlorophyll content at RLI-60 was 2.58 times higher than that of RLI-20. In conclusion, light intensity might be the major factor affecting growth and physiological characteristics of F. saxatilis grown under canopy of tall tree species.

• The Korean Journal of Community Living Science
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• 제16권4호
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• pp.47-57
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• 2005

To investigate the characteristics of edible flowers as a food material, we have examined the kinds, colors, sizes, fresh weights, pigments and shipping periods of edible flowers marketed on the cropping farms, selling agencies and Internet shopping malls from February through September, 2005. Thirty six kinds of edible flowers were marketed in Korea, and all but the chrysanthemum were introduced species. The characteristics of edible flowers were shown differently by the varieties following the same kinds of flowers. Those colors were yellow (twenty five kinds), red (twenty three), pink (twenty), white (eighteen), and orange (sixteen). Flower diameters were measured and showed that seven kinds of edible flowers were 1.0 to 2.0cm, fourteen 2.0 to 3.0cm, sixteen 3.0 to 4.0cm, eight 4.0 to 5.0cm, and nine over 5.0cm. Flower fresh weights were measured as follows: twenty one kinds of edible flowers were under 0.5g ($58.3\%$), eight were $0.6\∼1.0g(22.2\%$), and six were $1.1{\∼}1.5g(16.7\%$). The taste of edible flowers was often bitter (twenty one kinds), sweet and sour (seven), somewhat fragrant (six), fishy (three), and others (nine). The pigments of edible flowers were anthoxanthin (twenty seven kinds), flavonoid (twenty three), carotenoid (seventeen), and betanidin (four).

• KIPS Transactions on Software and Data Engineering
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• 제11권1호
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• pp.41-50
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• 2022

In this paper, a method to directly calculate the major elements of skin color such as melanin and hemoglobin from the RGB signal of the camera is proposed. The main elements of skin color typically measure spectral reflectance using specific equipment, and reconfigure the values at some wavelengths of the measured light. The values calculated by this method include such things as melanin index and erythema index, and require special equipment such as a spectral reflectance measuring device or a multi-spectral camera. It is difficult to find a direct calculation method for such component elements from a general digital camera, and a method of indirectly calculating the concentration of melanin and hemoglobin using independent component analysis has been proposed. This method targets a region of a certain RGB image, extracts characteristic vectors of melanin and hemoglobin, and calculates the concentration in a manner similar to that of Principal Component Analysis. The disadvantage of this method is that it is difficult to directly calculate the pixel unit because a group of pixels in a certain area is used as an input, and since the extracted feature vector is implemented by an optimization method, it tends to be calculated with a different value each time it is executed. The final calculation is determined in the form of an image representing the components of melanin and hemoglobin by converting it back to the RGB coordinate system without using the feature vector itself. In order to improve the disadvantages of this method, the proposed method is to calculate the component values of melanin and hemoglobin in a feature space rather than an RGB coordinate system using a feature vector, and calculate the spectral reflectance corresponding to the skin color using a general digital camera. Methods and methods of calculating detailed components constituting skin pigments such as melanin, oxidized hemoglobin, deoxidized hemoglobin, and carotenoid using spectral reflectance. The proposed method does not require special equipment such as a spectral reflectance measuring device or a multi-spectral camera, and unlike the existing method, direct calculation of the pixel unit is possible, and the same characteristics can be obtained even in repeated execution. The standard diviation of density for melanin and hemoglobin of proposed method was 15% compared to conventional and therefore gives 6 times stable.