• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.121-129
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• 2011

As the molecular breeding was progressed, many plant transformation techniques were attained for improving transformation accuracy and used to produce useful transgenic plants. Day by day, new varieties were developed so new transformation techniques required for these newly developed varieties. Carnation (Dianthus caryophyllus L.) is a popular and economically important ornamental plant, all over the world. Keeping this in view, we selected 18 varieties of D. caryophyllus L. commonly available in the market and did optimization of culture conditions for more efficient tissue culture and to get higher number of plants via micro-propagation. Four varieties namely Yellowdotcom, Jakarta, Belmonte, Polartessino etc. were selected for organ culture studies from single cell line. The optimum growth was recorded in the MS media supplied with sucrose 3%, NAA 1.0 mg/L and TDZ 1.0 mg/L. except Belmonte, in which, BA 1.0 mg/L was found to be the best combination, in place of TDZ, rest ingredients were same. The most efficient coagulating agent used to obtain higher number of plant from callus was phytagel 0.3%. The most effective explant for higher shoot formation was stem in which 80.2% shoot formation was recorded. It also reduced culture periods by 6 days.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.268.2-268
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• 1998

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.266.1-266
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• 1997

No Abstract, See Full Text

• Journal of Bio-Environment Control
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• v.14 no.3
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• pp.212-217
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• 2005

This experiment was conducted to investigate the effect of water soluble fertilizer(WSF) and slow release fertilizer(SRF) on the growth of carnation(Dianthus caryophyllus 'Invitation') cultured in C-channel mat irrigation system. Plants grown in $0.8{\~}1.0\;g{\cdot}L^{-1}$ of WSF showed the highest quality, especially on fresh and dry weight of aerial part, leaf number, total leaf area, plant height, and branch number. All plants showed increase of growth rate around 60 days after treatment, although there was a different increase rate. Total leaf area decreased its increase rate after 90 days after treatment. Carnations supplied by WSF showed better growth and quality than SRF, and the optimum concentration range of WSF for pot-carnation was $0.8{\~}1.0\;g{\cdot}L^{-1}$ in C-channel mat irrigation system.

• The Korean Journal of Mycology
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• v.17 no.4
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• pp.229-232
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• 1989

A blight disease of carnation (Dianthus caryophyllus L.) caused by Alternaria dianthi was found in greenhouses and vinylhouses in southern Korea. In severely affected beds, many carnation plants were blighted and died. The growth of the fungus id vitro was optimum between 24 and $28^{\circ}C$. In controlled inoculations, A. dianthi caused lesions on leaves and stems of carnation plants.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.190-197
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• 1997

This study was conducted to determine the optimum hydroponic system and nutrient solution for promoting the growth and flowering of hydroponically grown carnation. DFT (deep flow technique) and NFT (nutrient film technique) systems were employed and four kinds of nutrient solution, which are Cooper solution, Yamasaki solution, balanced nutrient solution by Japanese Horticultural Experiment Station and PTG solution in the Netherlands were used and compared. pH was lowest in the PTG solution compared to the other nutrient solutions used. Plant height was highest in the DFT system in the plot of PTG solution as of 53.0 cm at 88 days after transplanting, while lowest in NFT system with Cooper solution. Stem diameter was more thickened in NFT system with PTG solution as of 7.2 mm at 88 days after transplanting. Days to flowering was shortened to 122.3 days in NFT system when PTG solution was used. This results suggested a combination of NFT system with PTG solution as optimum hydroponic system and nutrient solution for cut carnation production. Further trials as to the management of nutrient solution by glowing stages should be followed.

• Korean Journal Plant Pathology
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• v.10 no.4
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• pp.314-318
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• 1994

Incidence of stem rot on carnation (Dianthus caryophyllus L.) ranged 11 to 29% in Namwon and Chongup area during the growing seasons from 1993 to 1994. Among 129 isolates from carnations in Namwon, 77 isolates were identified as Rhizoctonia solani, 38 isolates were Fusarium oxysporum and 14 isolates were not identified. Among 169 isolates in Chongup, 19 isolates were identified as R. solani, 106 isolates as F. oxysporum and 44 isolates were not identified. Among 77 isolates of R. solani isolated from the specimens of Namwon, 52 isolates were classified as anastomosis group AG 2-2 by anastomosis test, 14 isolates as AG 2-1 and 11 isolates as AG 4. Among 19 isolates from specimens of Chongup, 14 isolates were classified as anastomosis group AG 2-2 and 5 isolates as AG 4. Pathogenicity tests revealed that isolates of F. oxysporum and R.solani AG 2-2 were highly virulent and isolates of R.solani AG 2-1 and AG 4 were mildly virulent.

• FLOWER RESEARCH JOURNAL
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• v.26 no.4
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• pp.166-178
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• 2018

In this study, the principal objective was to investigate the effect of light quality and vessel ventilation on the growth and development, physiology, activities of antioxidant enzymes, and contents of mineral nutrients of carnation (Dianthus caryophyllus L.) 'Marble Beauty'. Single node cuttings stuck into the plant growth regulator (PGR)-free MS medium in containers covered with caps with or without a ventilation filter were cultured first four weeks under white and then additional four weeks under either white (control), blue, red, or red + blue light emitting diodes (LEDs) for 56 days. Interestingly, a ventilated culture condition not only reduced the percentage of the hyperhydricity, but also increased the total chlorophyll content (Chl a + Chl b) of the plantlets as compared to the non-ventilated condition. In addition, blue LEDs produced plantlets with the greatest number of shoots and red LEDs produced plantlets with the greatest shoot length. The quality of plantlets was improved under a ventilation condition. Besides, under a ventilated condition, red + blue LEDs raised APX activity, and blue LEDs not only raised the activity of the CAT, but also increased tissue contents of such elements as K, Ca, Mg, Zn, Mn and Fe. The red LEDs increased contents of B and Si under a ventilated condition, and Na accumulation under a non-ventilated condition. Thus, including blue or red LEDs as the light source in a ventilated culture condition will produce plantlets of carnation 'Marble Beauty' in vitro with improved quality.

• The Plant Pathology Journal
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• v.17 no.2
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• pp.101-105
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• 2001

A disease survey on the carnation (Dianthus caryophyllus L.) wilt was conducted during the high temperature period (June through August) and the low temperature period (February through May) in 58 greenhouses of its major cultivation areas, including Pusan, Kimhae, and Changwon in Korea from 1998 to 1999. The disease incidence was averaged 5.4% and 11.9% in the low and high temperature periods, respectively. Severe damage was found in summer with high incidences of around 50% in some greenhouses. Close examination of the symptoms and isolation of the causal agent revealed that there was a new disease different from Fusarium wilt caused by Fusarium oxysporum f. sp. dianthi, which was determined as the stub dieback caused by F. was cetermined as the stub dieback caused by F. graminearum (teleomorph : Gibberella zeae). The stub dieback symptoms involved brown rot of stem that started usually from the portion of cutting without discoloration of inner vascular tissues. Seven out of 38 isolates from the wilted plants were identified as F. graminearum, while the others as F. oxysporum f. sp. dianthi. Mycological characteristics of the stub dieback pathogen including colony color, absence of microconidia, and the shape of macroconidia, were consistent with F. graminearum previously described. This is the first report of the carnation stub dieback in Korea.

• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.50-56
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• 2010

This experiment was carried out to examine the effect of a silicate fertilizer on the growth and development of potted plants. Cutting of Kalanchoe blossfeldiana 'Kaluna' and 'Taos', and Dianthus caryophyllus L. 'Kazan' and 'Tula' were grown in 50 and 128-cell plug trays, respectively. Rooted cuttings transplanted to the mixture of a commercial medium and perlite (5:1, v/v) supplemented with a silicate fertilizer at 0, 40, 80, 120 or $160g{\cdot}L^{-1}$ medium was evaluated. A silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in the greatest plant height, leaf thickness, and root fresh and dry weights in both kalanchoe and carnation. However, plant height was suppressed in the treatment of a silicate fertilizer supplementation at higher concentrations in both kalanchoe and carnation. According to the scanning electron microscope images of transversal sections of tissues of roots and leaves in kalanchoe and carnation, the treatment of a silicate fertilizer supplementation at $40g{\cdot}L^{-1}$ medium resulted in plants with more compact tissue than the control.