• 제목/요약/키워드: Capsule transport

검색결과 14건 처리시간 0.021초

Further Evidence for the Role of Cantharidin in the Mating Behaviour of Blister Beetles (Coleoptera: Meloidae)

  • Nikbakhtzadeh, Mahmood Reza;Hemp, Claudia;Ebrahimi, Babak
    • Animal cells and systems
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    • 제11권2호
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    • pp.141-146
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    • 2007
  • Cantharidin is produced by blister beetles (Coleoptera: Meloidae) and smaller oedemerid beetles (Coleopetra: Oedemeridae) and is found in hemolymph and various tissues. The function of cantharidin in the courtship behaviour of meloids had never been fully established. Our studies show a correlation between density of cuticular pores and cantharidin titre of the scape and pedicel segments of male specimens of the East African species of Epicauta nyassensis (Haag-Rutenberg, 1880) (Coleoptera: Meloidae). Light microscopy of semi-thin cross sections of the male scape and pedicel indicates that there are many canal shaped structures that stretch from the antennal hemolymph to the antennomere surface. These structures may be tubules, which transport cantharidin circulating in the hemolymph to the surface, where the compound can be released via cuticular pore openings. Analyses of the head capsule and antennal segments of E. nyassensis females which had been copulated with males revealed low titre of cantharidin in the first two antennal segments. The density of the scape and pedicel pores of females was to some extent higher than the density of these pores on flagellum; however it was considerably lower than that of the males. Interestingly, no tubular cell or other transport structures were found in the cross sectioning of the female antennomeres or on the integument surface. During mating, male antennomeres, as well as cantharidin containing pores which are located on the $1^{st}\;and\;2^{nd}$ antennomeres, come into direct contact with the female antennae and may release cantharidin to their surface. Female E. nyassensis may be able to discriminate the opposite sex with abundant reserves of cantharidin prior to mating. This is another evidence that cantharidin function in close range sexual selection.

CBRNE 재난 시 격리이송을 위한 장치 개발에 관한 탐색적 연구 (An exploratory study on the development of a device for isolation unit in CBRNE disaster)

  • Ham, Seung Hee;Park, Namkwun;Yoon, Myong O
    • 한국재난정보학회 논문집
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    • 제14권1호
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    • pp.65-71
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    • 2018
  • 본 연구에서는 CBRNE 사고 및 공격에 의한 대규모 재난 발생 시 오염원 확산을 최대한으로 방지하면서 환자상태별 최적의 이송환경을 제공하기 위한 격리캡슐을 개발하는데 있어 필요한 성능적 기능적 요구사항을 고찰하고자 하였다. 이를 위하여 기존에 개발된 제품들의 특성을 파악함으로써 현장에서 즉각 활용을 어렵게 하는 곤란요소를 보완 수정할 수 있는 방안을 제시하였다. 연구결과, 현장에서 즉각 활용을 용이하게 하기 위한 격리이송장치를 개발하기 위해서 (1)기본기능, (2)효율적인 현장활용성, (3)격리이송장치 인터페이스 및 모듈간 결합방안의 각 고려요소에 대하여 제시하였다.

Differential Responses of Three Cyanobacteria to UV-B and Cd

  • Atri, Neelam;Rai, Lal-Chand
    • Journal of Microbiology and Biotechnology
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    • 제13권4호
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    • pp.544-551
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    • 2003
  • Interactive effects of UV-B and Cd on growth, pigment, photosynthesis, and lipid peroxidation have been studied in Anabaena, Microcystis, and Nostoc; all the tested cyanobacteria showed differential sensitivity to different dosage of UV-B and Cd alone as well as in combination. Phycocyanin was severely affected by UV-B and Cd by all the strains; the degree of pigment bleaching was most pronounced in Anabaena followed by Microcystis and Nostoc. $UV-B_2+Cd_2$ produced nearly 83, 78, and 65% inhibition of phycocyanin in Anabaena, Microcystis, and Nostoc, respectively. The above treatment also significantly decreased the contents of Chl ${\alpha}$ and carotenoid. Presence of capsule in Microcystis protected the phycocyanin bleaching as compared to decapsulated cells. Laboratory-grown Microcystis revealed about 75 and 80% inhibition, following $UV-B_2+Cd_2$ treatment, respectively. in capsulated and decapsulated cells. Damage caused by Cd was more pronounced than UV-B. Inhibition of photosynthesis did occur in all the test strains, being maximum in Anabaena. PS II was the most sensitive component of the electron transport chain, showing 84, 80, and 70% inhibition in Anabaena, Microcystis, and Nostoc, respectively. As compared to control, significant lipid peroxidation (6.5-fold higher) was observed in Anabaena with $UV-B_2+Cd_2$, $^{14}C-uptake$ was more susceptible to Cd and Uv-B than oxygen-evolution. Approximately 84, 80, and 76% inhibition of $^{14}C-uptake$ was observed in Anabaena, Microcystis, and Nostoc, respectively. Similarly, $UV-B_2+Cd_2$ inhibited APT content of Anabaena by 87%. This ,study suggests that inhibition of carbon fixation was due to decreased ATP content of the test cyanobacteria by UV-B+Cd, where Anabaena was the most sensitive and Nostoc the most tolerant.

분화중인 흰쥐 콩팥의 요세관에서 nNOS의 발현 (Expression of Neuronal Nitric Oxide Synthase (nNOS) in Developing Rat Kidney)

  • 송지현;류시현;김진;정주영
    • Applied Microscopy
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    • 제38권2호
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    • pp.141-148
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    • 2008
  • Nitric oxide는 콩팥에서 혈류의 조절, 사구체 혈액역동학 및 요세관의 운반에 중요한 역할을 하는 인자로서 최근 세포의 주기조절, 유사분열에도 관여하는 것으로 알려져 있다. 최근 연구 결과에 의하면 발생중인 콩팥에서의 nNOS의 발현은 성체와는 다른 것으로 알려져 있으나, 발생중인 콩팥에서의 발현 양상 및 발현시기 등에 대해서 알려진 바 없다. 따라서 본 연구에서는 발생중인 콩팥을 이용하여 분화중인 요세관에서 nNOS의 발현양상을 관찰하고자 하였다. 성체에서 nNOS의 면역반응성은 사구체 보우만주머니의 벽측상피와 치밀반, 내림가는부분 및 수질집합관에 발현되었다. 발생중인 콩팥에서는 임신 15일에 처음 발현하기 시작하였으며, 그 부위는 원위세관의 굵은오름부분이었다. 임신 20일에서 출산후 14일까지 새로이 형성되는 요세관의 굵은오름부분에서 관찰되었으나, 분화를 마친 요세관에서는 그 발현 양상이 감소하였다. 출생후 21일경에는 최종적으로 치밀반에만 국한되어 발현되었다. 속수질에서는 내림가는 부분과 속수질 집합관에서 발현되었으며 그 양상은 수질 중간부위에서 강하게 발현되었으며 시작부위와 말단부위에서는 약하게 발현되었다. 이상의 결과로 보아 굵은오름부분에서의 nNOS의 발현은 요세관의 분화정도와 일치하며 특히 그 발현양상이 분화가 활발한 시기에 강하게 발현되고 분화가 저하된 후에는 발현이 감소하는 것으로 보아 발생중인 콩팥의 성장에 중요한 역할을 할 것으로 생각된다.