• Archives of Pharmacal Research
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• v.27 no.11
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• pp.1154-1160
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• 2004

We investigated the neurotoxic effects of capsaicin (CAP) on pain sensitivity and on the expression of capsaicin receptor, the vanilloid receptor (VR1), in rats. High-dose application of CAP has been known to degenerate a large fraction of the sensory neurons. Although the neurotoxic effects of CAP are well documented, the effects of CAP on the vanilloid receptor (VR1) are not yet known. In this paper, we investigated the effects of high-dose application of CAP on the expression of VR1 in rats. Thermal and mechanical pain sensitivity was reduced when neonatal rats were treated with a high dose of CAP. This reduction of pain sensitivity was significantly decreased after initiating carrageenan-induced inflammation. The expression of VR1 in dorsal root ganglia (DRG) isolated from the CAP-treated rats was reduced compared to that from the vehicle-treated rats. Therefore, we can conclude that the neurotoxic effect of CAP is related to the decrease of VR1 expression.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.125-125
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• 2018

고추는 우리나라에서 가장 중요한 채소 작물이다. 최근에는 소비자 기호의 변화, 기능성 물질에 대한 수요의 증대에 따라 신품종 개발을 위한 다양한 유전자원의 수집이 점차적으로 중요해지고 있다. 특히, 유전자원을 활용하여 고기능성 물질 함유 품종 등 차별화된 신품종 개발을 할 필요가 있다. 본 과제의 주요 연구내용은 고추에서 매운맛을 내는 Capsaicin, Dihydrocapsaicin의 함량을 분석하여 매운맛이 우수한 유전자원을 선발하였다. 시험재료로 사용된 고추 유전자원의 종은 Capsicum annuum 375점, C. baccatum 7점, C. chinense 29점, C. frutescens 6점, C. sp. 46점으로 구성되었다. 분석된 고추 유전자원의 원산지는 43개국이며, 중국 원산 자원이 132점으로 가장 많이 분포하였고 원산지 미상 자원은 38점이었다. 대조품종으로 청양, 독야청청, 오로벨을 사용하였다. Capsaicin의 함량은 0~680 mg/100g/DW로 분포하였고, 매운맛 대조품종으로 쓰인 청양이 221.85 mg/100g/DW이었으며 분석된 고추 유전자원 중에 청양보다 높은 자원은 62자원이었다. Dihydrocapsaicin의 함량은 0 ~ 415mg/100g/DW로 분포하였고, 대조품종으로 쓰인 청양이 50.14 mg/100g/DW 이었으며 분석된 고추 유전자원 중에 청양보다 높은 자원은 197자원이었다.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.28 no.4
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• pp.41-50
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• 2015

Objectives : 선태는 아토피 피부염에서 소양증 완화를 위해 사용되고 있다. 본 연구에서는 면역계 및 신경계 손상을 일으킨 rat model에서 선태 추출물이 소양증 완화에 효과가 있는지 알아보고자 한다.Methods : 출생 48시간 이내의 rat을 대상으로, capsaicin(50 mg/kg)을 피하 투여하였다. 임의로 선정된 12마리의 실험군에 3주 동안 선태 추출물(0.5g/kg)을 매일 경구 투여하였다. 이후 scratching behavior 와 dermatitis score를 측정하였다.Results : 선태 투여군과 대조군에서 scratching number 와 dermatitis score의 차이가 없었다.Conclusions : 위의 결과로부터 capsaicin으로 유발한 아토피 피부염 rat model에서 선태의 소양증 완화 효과가 없다는 것을 알 수 있었다. 아토피 피부염의 효과적인 치료를 위해 면역계 뿐만 아니라 신경계 손상 회복시키는 약물을 찾기 위한 더 많은 연구가 필요할 것으로 생각된다.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.11b
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• pp.143-143
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• 2002

Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a pungent ingredient of hot chili peppers, has been reported to possess substantial anticarcinogenic and antimutagenic activities. In our previous study, we found that capsaicin (100 ${\mu}$M) induced significant inhibition of matrix metalloproteinase-2 activity by gelatin zymography, and that capsaicin (i.p., 2.5mg/kg) inhibited development of lung colonization (58%) in experimental lung metastasis assay.(omitted)

• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.301-305
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• 1991

Capsaicin content and redness were significantly different between home-made and factory-made kimchi whereas those were not significantly different in kochujang samples. Factory-made kimchi was 3 times higher in capsaicin content than home-made kimchi. In experimental kimchi and kochujang made with different levels of red pepper, content of capsaicins increased in proportion to the addition level of red pepper. It should be possible to estimate the amount of red pepper used in kimchi or kochujang if their capsaicin contents are determined.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.180.2-181
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• 2003

Capsaicin (8-methyl-N-vanillyl-6-nonenamide), a pungent ingredient of hot chili peppers, has been reported to possess substantial anticarcinogenic and antimutagenic activities. In the present study. we investigated the effects of capsaicin on pulmonary metastasis of B 16-F10 murine melanoma cells. as well as its mechanism of action. Capsaicin (i.p.. 2.5mg/kg) suppressed the number of lung colonies (58％) in experimental lung metastasis assay. (omitted)

• Proceedings of the Korean Biophysical Society Conference
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• 1998.06a
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• pp.30-30
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• 1998

The effects of capsaicin on phospholipase (PLC)-induced capacitative $Ca^{2＋}$ entry (CCE) were investigated in PC12 cells. Capsaicin inhibited one of PLC-linked signal, extracellular ATP-induced norepinephrine secretion with a concentration range, 10-100 $\mu$M. Capsaicin did not inhibit the peak portion of extracellular ATP-mediated [Ca$^{2＋}$]i rise, but it recovered more rapidly to the normal level of [Ca$^{2+}$]i from the sustained [Ca$^{2+}$]i level.(omitted)ted)

• The Korean Journal of Physiology and Pharmacology
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• v.11 no.5
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• pp.215-219
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• 2007

Small and large conductance $Ca^{2+}$-activated $K^+(SK_{Ca}\;and\;BK_{Ca})$ channels are implicated in the modulation of neuronal excitability. We investigated how changes in peripheral $K_{Ca}$ channel activity affect mechanical sensitivity as well as the afferent fiber type responsible for $K_{Ca}$ channel-induced mechanical sensitivity. Blockade of $SK_{Ca}$ and $BK_{Ca}$ channels induced a sustained decrease of mechanical threshold which was significantly attenuated by topical application of capsaicin onto afferent fiber and intraplantar injection of 1-ethyl-2-benzimidazolinone. NS1619 selectively attenuated the decrease of mechanical threshold induced by charybdotoxin, but not by apamin. Spontaneous flinching and paw thickness were not significantly different after $K_{Ca}$ channel blockade. These results suggest that mechanical sensitivity can be modulated by $K_{Ca}$ channels on capsaicin-sensitive afferent fibers.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.1015-1018
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• 1998

Capsaicin(8-methyl-N-vanillyl-6-nonenamide: CAP) known well as a major compound of not taste in hot pepper, was investigated for the inhibition effect on in vitro nitrosation. CAP(100$\mu$mol) inhibited the formation of N-nitrosoproline(NPRO) and N-nitrosothioproline(NTPRO) by 56% and 26%, respectively. Vanillyl alcohol inhibited the nitrosation of proline by a concentration-dependent manner, and vanillic acid and vanillin were less effective in blocking the nitrosation of proline compared to CAP and anillyl alcohol. The inhibitory effect of NPRO formation by CAP was evaluated to similar with alpha-tocopherol, and vanillyl alcohol was more effective than alpha-tocopherol in blocking the nitrosation of proline. Our results suggested that CAP and its metabolites such as vanillyl alcohol could inhibit endogenous nitrosation in hydrophobic biological environment.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.128.1-128.1
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• 2003

VR1, a capsaicin receptor, is now known to playa major role in mediating inflammatory thermal nociception. Although the physiological role or biophysical properties of VR1 are known, its activation mechanisms by ligands are poorly understood. Here, we show that VR1 requires phosphorylation by $Ca^{2+}$-calmodulin-dependent kinase II (CaMKII) for its activation by capsaicin. In contrast, dephosphorylation by calcineurin, leads to desensitization of the receptor. Point mutation of VR1 at two putative consensus sites for CaMKII fails to elicit capsaicin-sensitive currents with concomitant reduction in phosphorylation of VR1 in vivo. (omitted)