• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1666-1673
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• 2011

The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.765-769
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• 2015

Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.

• 대한수의학회지
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• 제50권1호
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• pp.25-28
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• 2010

Caseous lymphadenitis (CLA) is a chronic and contagious disease of sheep and goats caused by Corynebacterium (C.) pseudotuberculosis. A four-year-old female Saanen dairy goat was submitted to the Animal Disease Diagnostic Center at National Veterinary Research and Quarantine Service. The clinical signs of the goat were emaciation, abortion and quadriplegia. The multifocal nodules of lymph nodes were encapsulated and filled with whitish caseous contents on the cut surface. Histopathologically, lymph nodes displayed suppurative and necrotizing granulomas. Caseous necrosis was diffusely observed in the center of the lymph nodes. Gram positive bacilli were shown in the lesions. C. pseudotuberculosis was isolated and confirmed by the biochemical tests and PCR assay. Based on clinical signs, histopathological examination and bacterial isolation, we diagnosed this case as CLA. To our knowledge, this is the first report of CLA in a Saanen dairy goat in Korea.

• 한국가축번식학회지
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• 제21권4호
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• pp.373-379
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• 1997

This study was carried out to determine the hormone treatment scheme for an efficient superovulation and optimal recovery time for obtaining pronuclear embryos suitable for DNA injection in Korean native goats. For a superovulation, FSH(5.6mg) was given over four days in twice daily injections with (FSH/hCG group) or without(FSH group) hCG(100 IU) co-injection at the time of 7th FSH injection. Estrus cycle was synchronized by norgestomet ear-implantation for 11 days and its removal at the time of 6th FSH injection. Among the treated goats, the percentage of ovulated goats, which were examined at 70 to 76 h following implant removal, was greater in FSH/hCG group than in FSH group (100% vs 36.4%) but there was no significant difference in the mean numbers of ovulation points and fertilization rates between the two groups. To optimize hCG treatment scheme and recovery time, we injected hCG at the time of 7th (FSH/hCGa) or 8th(FSH/hCGb) FSH injection and then examined the developmental stage of the embryos recovered at different times after implant removal. In FSH/hCGa group, significant portions(31 to 44%) were beyond 1-cell stage, which was non-injectable, irrespective of their recovery time. However, in FSH/hCGb group recovered at 70 to 76 h after implant removal, great portions(69%) were fertilized and most of them(96.6%) were injectable 1-cell stage. Considering together the fertilization rate and developmental stage of recovered embryos, it is recommendable to administrate hCG at the time of final 8th FSH injection and collect the embryos at 70 to 76 h after implant removal to obtain injectable embryos as many as possible in Korean native goats.

• 대한수의학회지
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• 제53권4호
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• pp.273-276
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• 2013

Two Korean black goat (approx. 2 and 3 years old) showing diarrhea and chronic weight loss were submitted to Animal and Plant Quarantine Agency. At necropsy, there were thickening of small intestine and enlargement of mesenteric lymph nodes. Microscopically, they had granulomatous enteritis in the small and large intestine and granulomatous lymphadenitis. By polymerase chain reaction (PCR) and acid fast stain, strong positive reaction and acid-fast rod bacteria were detected. According to the result of histopathology and PCR, we confirmed this case as Johne's disease. As far as we know, this is the first report of Johne's disease in Korean black goat.

• 대한수의학회지
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• 제53권1호
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• pp.55-60
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• 2013

The vomeronasal organ (VNO) plays an important role in reproduction and social activities in ruminants including goats. A morphological study on the structure of VNO and its epithelial cells was carried out in Korean black goats. Grossly, the VNO of Korean goats opens into mouth through incisive ducts. Microscopically, the epithelium of VNO consisted of medial sensory epithelium and lateral non-sensory epithelium. Several blood vessels and nerve bundles were observed in the lamina propria encased by vomeronasal cartilage. Immunohistochemical staining showed that protein gene product (PGP) 9.5 was immunostained in the receptor cells of the sensory epithelium and in some cells of the non-sensory epithelium. Galectin-3 was mainly observed in the supporting cells of sensory and non-sensory epithelium. Lectins including wheat germ agglutinin, Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin Isolectin B4, Dolichos biflorus agglutinin and soybean agglutinin used in this study were bound in VNO sensory, non-sensory epithelia as well as in the lamina propria with varying intensity. Collectively, this is a first descriptive morphological study of VNO of Korean black goat with special reference to lectin histochemistry.

• 한국초지조사료학회지
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• 제29권1호
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• pp.73-82
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• 2009

본 연구는 2008년 1월부터 12월까지 충남대 학교 농업생명과학대학과 가축유전자원시험장에서 수행하였다. 공시가축은 유산양(Sannen, Capra aegagrus) 12두(female, $30{\pm}1.8kg$), 재래산양(Korean native goats, Capra hircus) 12두(female, $24{\pm}2.4kg$), 꽃사슴(sika deer, Servus nippon) 5두(female, $92{\pm}5.2kg$)를 공시하였다. 공시재료는 2006년에 미국에서 수입된 annual ryegrass straw, perennial ryegrass straw, tall fescue straw 및 Kentucky bluegrass straw 등 4종과, 2007년에 미국에서 수입된 alfalfa hay, bermudagrass hay, timothy hay, klein grass hay, oat hay 및 orchardgrass hay 등 6종 등 총 10종과 대조구로 국내산 혼합 목건초(mixed hay) 등 총 11종을 공시하였으며 얻어진 결과는 다음과 같다. 수입건초는 종류에 따라 화학적 성분과 건물소화율(in vitro digestibility, IVDMD)는 현저한 차이를 보였으며 (p<0.05), alfalfa hay와 orchard-grass hay를 제외하고는 대부분의 수입건초는 대조구에 비하여 조단백질(crude protein, CP)함량과 IVDMD가 낮은 반면에, NDF와 ADF 같은 섬유소물질의 함량은 상당히 높은 결과를 나타내었다. RFV(relative feed value)는 수입건초의 종류에 따라 차이를 보였으며, 대부분의 수입건초의 RFV는 $4{\sim}5$등급에 해당되었다. 유산양, 재래산양 및 꽃사슴에 의한 채식량과 기호성은 수입건초의 종류에 따라 현저한 차이를 나타내었으며, 공통적으로 orchardgrass hay와 bermudagrass hay가 높았던 반면에, Kentucky bluegrass straw, tall fescue straw, annual ryegrass straw 및 perennial ryegrass straw 등은 채식량과 기호성 순위가 상당히 낮은 것으로 밝혀졌다. 이상의 결과로 보아, 공시된 수입건초는 종류에 따라 품질과 기호성이 현저한 차이를 보이고 있어 수입건초의 품질평가에 대한 기준설정이 중요하다고 하겠다.