• Title/Summary/Keyword: Capillary method

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Precise correction of the copper emission spectra from the pulsed plasma jet (펄스 플라즈마 제트내에 있는 구리원자의 발광 스펙트럼 정밀 보정)

  • 김종욱;고동섭;오승묵
    • Korean Journal of Optics and Photonics
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    • v.12 no.2
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    • pp.115-120
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    • 2001
  • In the present study, we described in detail a precise correction method of the copper emission spectra obtained from a highpressure and high-temperature pulsed plasma-jet. The pulsed plasma-jet is initiated from an electro-thermal capillary discharge through a small orifice, and expanded rapidly into an atmosphere. In order to characterize the plasma, fundamental measurements such as the plasma excitation temperature or electron number density are essential. However those spectral lines which are directly related to the excitation temperature or electron number density may be distorted by the spectral response of the optical instruments used. Therefore, in this paper, we discuss some efforts to derive precise correction methods of the copper emission spectra obtained from the pulsed plasma-jet. a-jet.

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Curcumin supplementation and delayed onset muscle soreness (DOMS): effects, mechanisms, and practical considerations

  • Yoon, Wan-Young;Lee, Kihyuk;Kim, Jooyoung
    • Korean Journal of Exercise Nutrition
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    • v.24 no.3
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    • pp.39-43
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    • 2020
  • [Purpose] In this literature review we aimed to investigate the effects of curcumin supplementation on delayed onset muscle soreness (DOMS), which occurs after exercise, and evaluate related parameters to propose practical recommendations for the field of exercise physiology. [Methods] Experimental studies conducted on curcumin supplementation and DOMS were systematically reviewed to determine (1) the effect of curcumin supplementation on DOMS, (2) potential mechanisms by which curcumin supplementation may attenuate DOMS, and (3) practical considerations for curcumin supplementation. [Results] While several studies have reported that curcumin supplementation attenuates DOMS after exercise, others have reported that curcumin supplementation has no effect on DOMS. Several mechanisms have been proposed by which curcumin supplementation may attenuate DOMS; the most probable of which is a reduction in inflammatory response. Other potential mechanisms include modulation of transient receptor potential vanilloid 1 (TRPV1) or changes in post-exercise capillary lactate levels; these require further examination. The usual recommended dose of curcumin is 150-1500 mg daily (sometimes up to 5 g), divided into 2-3 portions and taken before and after exercise. It is not necessary to take curcumin together with piperine. [Conclusion] Although conflicting results regarding the effects of curcumin supplementation on DOMS exist in literature, it may be considered as a method of nutritional intervention for reducing post-exercise DOMS.

Controlling interlayer spacing of GO membranes via the insertion of GN for high separation performance

  • Xuan Liu;Zhu Zhou;Hengzhang Dai;Kuang Ma;Yafei Zhang;Bin Li
    • Membrane and Water Treatment
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    • v.14 no.3
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    • pp.107-114
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    • 2023
  • Graphene oxide (GO) membranes have attracted extensive attention in water treatment and related fields. However, GO films are unstable and have low permeability, which have hindered their further development. In this paper, a simple and effective method was used in which GO and single-layer graphene (GN) were mixed, and the layer spacing was effectively controlled by accurately controlling the ratio of GO to GN. GO-GN composite membranes have excellent stability, salt rejection (95.4%), and water flux (26 L m-2 h-1 bar-1). This unique design structure can be used for precise and effective regulation of the layer spacing in GO, improving the rejection rate, and increasing water flux via the enhancement of low-friction capillary action. The rational development and use of this unique composite membrane provides a reference for the water treatment field.

Rapid Diagnosis of CMT1A Duplications and HNPP Deletions by Multiplex Microsatellite PCR

  • Choi, Byung-Ok;Kim, Joonki;Lee, Kyung Lyong;Yu, Jin Seok;Hwang, Jung Hee;Chung, Ki Wha
    • Molecules and Cells
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    • v.23 no.1
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    • pp.39-48
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    • 2007
  • Charcot-Marie-Tooth (CMT) disease and hereditary neuropathy with liability to pressure palsies (HNPP) are frequent forms of genetically heterogeneous peripheral neuropathies. Reciprocal unequal crossover between flanking CMT1A-REPs on chromosome 17p11.2-p12 is a major cause of CMT type 1A (CMT1A) and HNPP. The importance of a sensitive and rapid method for identifying the CMT1A duplication and HNPP deletion is being emphasized. In the present study, we established a molecular diagnostic method for the CMT1A duplication and HNPP deletion based on hexaplex PCR of 6 microsatellite markers (D17S921, D17S9B, D17S9A, D17S918, D17S4A and D17S2230). The method is highly time-, cost- and sample-saving because the six markers are amplified by a single PCR reaction and resolved with a single capillary in 3 h. Several statistical and forensic estimates indicated that most of these markers are likely to be useful for diagnosing the peripheral neuropathies. Reproducibility, as determined by concordance between independent tests, was estimated to be 100%. The likelihood that genotypes of all six markers are homozygous in randomly selected individuals was calculated to be $1.6{\times}10^{-4}$, which indicates that the statistical error rate for this diagnosis of HNPP deletion is only 0.016%.

Development of the Organic Solar Cell Technology using Printed Electronics (인쇄전자 기술을 이용한 유기 태양전지 기술 개발)

  • Kim, Jungsu;Yu, Jongsu;Yoon, Sungman;Jo, Jeongdai;Kim, Dongsoo
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.113.1-113.1
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    • 2011
  • PEMS (printed electro-mechanical system) is fabricated by means of various printing technologies. Passive and active compo-nents in 2D or 3D such as conducting lines, resistors, capacitors, inductors and TFT(Thin Film Transistor), which are printed withfunctional materials, can be classified in this category. And the issue of PEMS is applied to a R2R process in the manu-facturing process. In many electro-devices, the vacuum process is used as the manufacturing process. However, the vacuum process has a problem, it is difficult to apply to a continuous process such as a R2R(roll to roll) printing process. In this paper, we propose an ESD (electro static deposition) printing process has been used to apply an organic solar cell of thin film forming. ESD is a method of liquid atomization by electrical forces, an electrostatic atomizer sprays micro-drops from the solution injected into the capillary with electrostatic force generated by electric potential of about several tens kV. ESD method is usable in the thin film coating process of organic materials and continuous process as a R2R manufacturing process. Therefore, we experiment the thin films forming of PEDOT:PSS layer and active layer which consist of the P3HT:PCBM. The organic solar cell based on a P3HT/PCBM active layer and a PEDOT:PSS electron blocking layer prepared from ESD method shows solar-to-electrical conversion efficiency of 1.42% at AM 1.5G 1sun light illumination, while 1.86% efficiency is observed when the ESD deposition of P3HT/PCBM is performed on a spin-coated PEDOT:PSS layer.

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Determination of Captan, Folpet, Captafol and Chlorothalonil Residues in Agricultural Commodities using GC-ECD/MS (GC-ECD/MS를 이용한 농산물 중 Captan, Folpet, Captafol 및 Chlorothalonil의 잔류분석법)

  • Lee, Su-Jin;Hwang, Young-Sun;Kim, Young-Hak;Kwon, Chan-Hyeok;Do, Jung-A;Im, Moo-Hyeog;Lee, Young-Deuk;Choung, Myoung-Gun
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.165-175
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    • 2010
  • A gas chromatographic (GC) method was developed to determine residues of captan, folpet, captafol, and chlorothalonil, known as broad-spectrum protective fungicides for the official purpose. All the fungicide residues were extracted with acetone containing 3% phosphoric acid from representative samples of five agricultural products which comprised rice, soybean, apple, pepper, and cabbage. The extract was diluted with saline, and dichloromethane partition was followed to recover the fungicides from the aqueous phase. Florisil column chromatography was additionally employed for final cleanup of the extracts. The analytes were then determined by gas chromatography using a DB-1 capillary column with electron capture detection. Reproducibility in quantitation was largely enhanced by minimization of adsorption or thermal degradation of analytes during GLC analysis. Mean recoveries generated from each crop sample fortified at two levels in triplicate ranged from 89.0~113.7%. Relative standard deviations (RSD) were all less than 10%, irrespective sample types and fortification levels. As no interference was found in any samples, limit of quantitation (LOQ) was estimated to be 0.008 mg/kg for the analytes except showing higher sensitivity of 0.002 mg/kg for chlorothalonil. GC/Mass spectrometric method using selected-ion monitoring technique was also provided to confirm the suspected residues. The proposed method was reproducible and sensitive enough to determine the residues of captan, folpet, captafol, and chlorothalonil in agricultural commodities for routine analysis.

Chiral Separation of Lactic Acid in Culture Media and Cells of Lactobacillus delbrueckii subsp. lactis as O-Pentafluoropropionylated (S)-(+)-3-Methyl-2-Butyl Ester by Achiral Gas Chromatography-Mass Spectrometry

  • Paik, Man-Jeong;Nguyen, Duc-Toan;Yoon, Jae-Hwan;Chae, Han-Seung;Kim, Kyoung-Rae;Lee, Gwang;Lee, Pyung-Cheon
    • Bulletin of the Korean Chemical Society
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    • v.32 no.7
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    • pp.2418-2422
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    • 2011
  • The enantiomeric separation of lactic acid for its absolute configuration has become important task for understanding its biological origin and metabolic process involved in the formation of lactic acid. It involves the conversion of enantiomers as diastereomeric O-pentafluoropropionylated (S)-(+)-3-methyl-2-butyl ester and the direct separation by gas chromatography-mass spectrometry on a achiral capillary column. The (R)- and (S)-lactic acids were completely separated with a high resolution of 1.9. The newly developed method showed good linearity (r ${\geq}$ 0.999), precision (% relative standard deviation = 3.4-6.2), and accuracy (% relative error = -7.7-1.4) with the detection limit of 0.011 ${\mu}g/mL$. When the method was applied to determine the absolute configuration of lactic acid in Lactobacillus delbrueckii subsp. lactis 304 (LAB 304), the composition (%) of (R)-lactic acid in the cell pellet and in the culture medium were $89.0{\pm}0.1$ and $78.2{\pm}0.4$, respectively. Thus, it was verified that the present method is useful for the identification and composition test of lactic enantiomers in microorganisms.

Genetic Diversity Analysis of Wood-cultivated Ginseng using Simple Sequence Repeat Markers (SSR 마커를 이용한 산양삼의 유전적 다양성 분석)

  • Gil, Jinsu;Um, Yurry;Byun, Jae Kyung;Chung, Jong Wook;Lee, Yi;Chung, Chan Moon
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.6
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    • pp.389-396
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    • 2017
  • Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

Growth and yield responses of rice varieties to various soil water deficit conditions under different soil types

  • Kikuta, Mayumi;Samejima, Hiroaki;Magoti, Rahab;Kimani, John M.;Yamauchi, Akira;Makihara, Daigo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.322-322
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    • 2017
  • To avoid drought stress under rainfed upland conditions, it is important for rice to efficiently utilize water at shallow soil layers supplied by rainfall, and access to water retained in deer soil layers. The root developmental characteristics of rice, which play important role in the adaptability to drought conditions, vary depending on the variety. Moreover, water availability for plant differs depending on the soil types that have different physical properties such as water holding capacity, permeability, capillary force, penetration resistance, etc. In this study, we evaluated growth and yield responses of rice varieties to various soil water deficit conditions under three different soil types. The experiment was conducted in a plastic greenhouse at the Kenya Agricultural and Livestock Research Organization-Mwea from October 2016 to January 2017. Two upland varieties (NERICA 1 and 4) and one lowland variety (Komboka) were grown in handmade PVC pots (15.2 cm diameter and 85.0 cm height) filled with three different types of soil collected from major rice-growing areas of the country, namely black cotton (BC), red clay (RC), and sandy clay (SC). Three watering methods, 1) supplying water only from the soil surface (W1), 2) supplying water only from the bottom of the pots (W2), and 3) supplying water both from the soil surface and the bottom of pots (W3), were imposed from 40 days after sowing to maturity. Soil water content (SWC) at 20, 40, and 60 cm depths was measured regularly. At the harvesting stage, aboveground and root samples were collected to determine total dry weight (TDW), grain yield, and root length at 0-20, 20-40, 40-60, and 60-80 cm soil layers. Irrespective of the watering methods, the greatest root development was obtained in RC, while that in BC was less than other two soils. In BC, the degree of yield reduction under W1 was less than that in RC and SC, which could be attributed to the higher water holding capacity of BC. In RC, the growth and yield reduction observed in all varieties under W1 was attributed to the severe drought stress. On the other hand, under W2, SWC at the shallow soil depth in RC was maintained because of its higher capillary force compared with BC and SC. As the result, growths and yields in RC were not suppressed under W2. In SC, deep root development was not promoted by W2 irrespective of the varieties, which resulted in significant yield losses. Under W1, the rice growth and yield in SC was decreased although shallow root development was enhanced, and the stomatal conductance was maintained higher than RC. It was suspected that W1 caused nutrients leaching in SC because of its higher permeability. Under rainfed conditions, growth and yield of rice can be strongly affected by soil types because dynamics of soil water conditions change according to soil physical properties.

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Development of Ceramic Membrane for Metal Ion Separation of Lignin Extract from Pulp Process (펄프공정으로부터 배출되는 리그닌 추출물의 금속이온 분리를 위한 세라믹 분리막 개발)

  • Shin, Min Chang;Choi, Young Chan;Park, Jung Hoon
    • Membrane Journal
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    • v.27 no.2
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    • pp.199-204
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    • 2017
  • In this study, a study was carried out for the separation of metal ions in lignin extract discharged from the pulp process. alumina powders were mixed with DMAc (N, N-dimethylacetamide) solvent and PESf (Polyethersulfone) polymer, PVP (polyvinylpyrrolidone) dispersant was added and slip casting method was used to prepare the membrane. The membrane was measured for pore size through a CFP (Capillary Flow Porometer) device and the surface and cross-section of the membrane were observed through a FE-SEM (Field Emission Scanning Electron Microscope). The flux was calculated by measuring the filtered weight per hour using a separation experiment device. Pore size measurements were performed under increasing pressure from 0 psi to 30 psi. The pore size of the membrane was $0.4{\mu}m$ and the flux decreased from the initial flux value of $6.36kg{\cdot}m^{-2}{\cdot}h^{-1}$ to $1.98kg{\cdot}m^{-2}{\cdot}h^{-1}$ due to the fouling of the membrane. After the permeation experiment, membrane contaminants were removed by simple washing. Separation experiments showed that Na contained in the initial lignin extract was reduced by 69%, Fe was removed by 87%, K by 95%, Ca by 93% and Mg by 96%.