• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.529-535
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• 2011

Single-chain variable fragment (scFv) is a fusion protein of the variable regions of the heavy (VH) and light (VL) chains of immunoglobulin, connected with a short linker peptide of 10 to about 20 amino acids. In this study, the scFv of a monoclonal antibody against the third domain of human CD4 was cloned from OKT4 hybridoma cells using the phage display technique and produced in E. coli. The expression, production, and purification of anti-CD4 scFv were tested using SDS-PAGE and Western blot, and the specificity of anti-CD4 scFv was examined using ELISA. A 31 kDa recombinant anti-CD4 scFv was expressed and produced in bacteria, which was confirmed by SDS-PAGE and Western blot assays. Sequence analysis proved the ScFv structure of the construct. It was able to bind to CD4 in quality ELISA assay. The canonical structure of anti-CD4 scFv antibody was obtained using the SWISS_MODEL bioinformatics tool for comparing with the scFv general structure. To the best of our knowledge, this is the first report for generating scFv against human CD4 antigen. Engineered anti-CD4 scFv could be used in immunological studies, including fluorochrome conjugation, bispecific antibody production, bifunctional protein synthesis, and other genetic engineering manipulations. Since the binding site of our product is domain 3 (D3) of the CD4 molecule and different from the CD4 immunological main domain, including D1 and D2, further studies are needed to evaluate the anti-CD4 scFv potential for diagnostic and therapeutic applications.

• Ocean and Polar Research
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• 제34권1호
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• pp.1-18
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• 2012

We investigated seasonal changes in the mesozooplankton community structure in November 2006 and February, May, and August 2007 at 12 stations in the semi-enclosed Muan Bay, Korea. Forty taxa were sampled, with an average abundance ranging from 1,459 to 20,078 indiv. $m^{-3}$; the highest abundance was detected in August 2007, and the lowest in February 2007. Acartia omorii, A. hudsonica, A. ohtsukai, Bestiolina coreana, Calanopia sp., Paracalanus parvus s. l., Cirripedia larvae, Decapoda larvae, and Gastropoda larvae were the most abundant taxa detected. The species diversity of the mesozooplankton was high around the inner regions in August 2007 but it was relatively low in November 2006. Non-metric multidimensional scaling (nMDS) revealed significant differences in the structure of mesozooplankton community among the seasons. Canonical correspondence analysis (CCA), performed to examine the relationships among dominant taxa, stations, and environmental variables, showed that most species of copepods were positively correlated with temperature, salinity, chlorophyll a, and COD concentration. Our results suggested that the mesozooplankton community structure observed in this study might be affected partly by the seasonal changes in environmental variables, such as the status of the sluice gates (i.e., open or closed) and the in situ production of resting eggs by major copepods.

• 정보관리연구
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• 제39권4호
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• pp.121-154
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• 2008

본 연구에서는 정보검색도구 관점에서 지식조직체계로서 기존 시소러스 구축방안의 특성과 한계점을 검토하고, 대상 정보의 지식구조의 반영 및 정보 간의 의미관계 추론을 지원하는 온톨로지 기반의 주제 색인어 구조화 방안을 제시한다. 기존의 용어 중심의 시소러스와 달리, 단계별 연구프로세스 과정에서 수행되는 연구자의 정보행위 및 수요 분석에 때라 주제색인어의 개념을 식별 범주화하고, 인문사회과학 분야 학술논문의 지식체계를 구성하는 그 개념들 간의 유기적 관계정의를 통해 주제 색인어 집합의 의미구조를 정형화하였다. 이를 기반으로 각각의 온톨로지 기반 주제 색인어 집합은 구조화된 의미 색인으로서 대상 문서의 지식체계를 표현한다. 정보수요에 따라 정의된 공리나 추론규칙을 활용하여 이용자는 문제 해결에 적합한 정보를 대상 정보의 의미관계로 구성된 주제 도메인의 학술커뮤니케이션 네트워크상에서 분석적 정보탐색을 통해 효율적으로 검색 할 수 있다.

• Molecules and Cells
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• 제39권4호
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• pp.316-321
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• 2016

The receptor activator of nuclear factor ${\kappa}B$ (RANK) and its ligand RANKL are key regulators of osteoclastogenesis and well-recognized targets in developing treatments for bone disorders associated with excessive bone resorption, such as osteoporosis. Our previous work on the structure of the RANK-RANKL complex revealed that Loop3 of RANK, specifically the non-canonical disulfide bond at the tip, performs a crucial role in specific recognition of RANKL. It also demonstrated that peptide mimics of Loop3 were capable of interfering with the function of RANKL in osteoclastogenesis. Here, we reported the structure-based design of a smaller peptide with enhanced inhibitory efficiency. The kinetic analysis and osteoclast differentiation assay showed that in addition to the sharp turn induced by the disulfide bond, two consecutive arginine residues were also important for binding to RANKL and inhibiting osteoclastogenesis. Docking and molecular dynamics simulations proposed the binding mode of the peptide to the RANKL trimer, showing that the arginine residues provide electrostatic interactions with RANKL and contribute to stabilizing the complex. These findings provided useful information for the rational design of therapeutics for bone diseases associated with RANK/RANKL function.

• 생태와환경
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• 제49권3호
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• pp.236-243
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• 2016

본 논문은 국립공원 아고산대 침엽수림을 효과적으로 보전하고 관리하는 데 기초자료로 활용하고자 진행된 사례연구로서 지리산국립공원과 속리산국립공원의 아고산대에서 현지 조사된 구상나무 (Abies koreana Wilson) 210개체의 서식실태 자료를 바탕으로, 입지환경의 기본이 되는 지리적 위치와 지형적 특성이 구상나무의 생장에 미치는 영향을 분석하였다. 이를 위해서 구상나무의 생장과 관련된 변수는 수고 및 흉고직경으로 하고, 지형적 특성은 GIS 공간분석을 이용하여 추출된 지리적 위도, 해발고도, 산지경사, 사면향 그리고 지형습윤지수로 하였다. 두 변수군의 연관성의 유무와 정도를 평가하기 위해서 정준상관분석을 이용하고, 다중회귀분석을 이용하여 지리 지형적 특성이 구상나무의 생장구조에 미치는 영향을 평가하였다. 구상나무 생장구조를 나타내는 흉고직경 및 수고는 지형의 수직적인 분포보다 지리 위도적인 분포와 연관성이 더 크고, 지리 지형요소는 수고보다 흉고직경과 연관성이 더 큰 것으로 나타났다. 구상나무의 생장구조변수와 지리 지형변수는 유의한 상관관계가 있고, 지리 지형변수가 생장구조변수를 18.1% 정도 설명하는 것으로 나타났다. 구상나무의 흉고직경에 영향을 주는 변수는 지리적 위도, 지형습윤지수, 사면향 그리고 해발고도의 순으로 통계적 유의성이 있는 것으로 나타났다. 위도가 높아질수록 흉고직경은 작아지고 지형적 요소에 따라서는 커지는 것으로 나타났다. 수고에 영향을 주는 변수는 지형습윤지수만이 유의미한 것으로 나타났다. 전반적으로, 구상나무의 생장구조와 관련된 수고와 흉고직경은 지리적인 특성의 영향이 가장 클 것으로 나타났다. 특히 지형적 특성 중에서 수분상태의 영향이 다른 지형요소에 비해서 더 클 것으로 예측되었다. 이러한 결과로 볼 때, 지리 지형적인 특성은 구상나무의 생장에 중요한 요인이 될 것으로 추측된다. 비록 지리 지형적 특성만을 고려하고 GIS를 이용하여 제작된 공간분석 자료를 사용한 한계가 있다고 해도, 본 연구결과는 향후 국립공원 아고산대에서 서식하고 있는 침엽수림의 생장환경을 조사하고 연구하는 데 유용하고, 구상나무 등 상록침엽교목을 효과적으로 관리하고 보전하기 위해서 대책을 수립하는 데 기초자료로 활용될 것으로 기대된다.

• BMB Reports
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• 제35권5호
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• pp.472-475
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• 2002

Phaseolus vulgaris phytohemagglutinin L is a homotetrameric-leucoagglutinating seed lectin. Its three-dimensional structure shows similarity with other members of the legume lectin family. The tetrameric form of this lectin is pH dependent. Gel filtration results showed that the protein exists in its dimeric state at pH 2.5 and as a tetramer at pH 7.2. Contrary to earlier reports on legume lectins that possess canonical dimers, thermal denaturation studies show that the refolding of phytohemagglutinin L at neutral pH is irreversible. Differential scanning calorimetry (DSC) was used to study the denaturation of this lectin as a function of pH that ranged from 2.0 to 3.0. The lectin was found to be extremely thermostable with a transition temperature around $82^{\circ}C$ and above $100^{\circ}C$ at pH 2.5 and 7.2, respectively. The ratio of calorimetric to vant Hoff enthalpy could not be calculated because of its irreversible-folding behavior. However, from the DSC data, it was discovered that the protein remains in its compact-folded state, even at pH 2.3, with the onset of denaturation occurring at $60^{\circ}C$.

• 대한화학회지
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• 제14권1호
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• pp.97-107
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• 1970

액체 구조의 천이상태 이론을 써서 고체 표면에 기체가 흡착된 계에 대한 상태합을 유도하였다. 이 상태합을 써서 아르곤, 질소, 벤젠 등이 여러 가지 흡착제에 흡착되었을 때의 흡착등온 곡선을 계산하였으며, 이 계산 값들은 측정치와의 좋은 일치를 보여 준다. 그리고 벤젠이 흑연 표면에 흡착되었을 때의 표면압, 물엔트로피, 물에너지, 몰흡착열 들을 계산하였다. 몰엔트로피는 표면이 단분자층으로 완전히 덮였을 때 최소값을 갖는다. 아울러 흡착에 관여된 여러 가지 물리적인 성질들의 계산 방법을 설명하였다.

• Bulletin of the Korean Chemical Society
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• 제27권12호
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• pp.1976-1980
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• 2006

We have calculated the reaction probability and the reaction cross-section of the $N(^4S)+O_2(X^3\sum_{g}^{-})\;\rightarrow\;NO(X^2\Pi)+O(^3P)$ reaction by the quasiclassical trajectory method with the 6th-order explicit symplectic algorithm, based on a new ground potential energy surface. The advantage of the 6th-order explicit symplectic algorithm, conserving both the total energy and the total angular momentum of the reaction system during the numerical integration of canonical equations, has firstly analyzed in this work, which make the calculation of the reaction probability more reliable. The variation of the reaction probability with the impact parameter and the influence of the relative translational energy on the reaction cross-section of the reaction have been discussed in detail. And the fact is found by the comparison that the reaction probability and the reaction cross-section of the reaction estimated in this work are more reasonable than the theoretical ones determined by Gilibert et al.

• Journal of electromagnetic engineering and science
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• 제13권2호
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• pp.73-85
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• 2013

In this paper we present an alternative approach to addressing the problem of designing cloaks for radar targets, which have been dealt with in the past by using the transformation optics (TO) algorithm. The present design utilizes realistic materials, which can be fabricated in the laboratory, and are wideband as well as relatively insensitive to polarization and incident angle of the incoming wave. The design strategy, presented herein, circumvents the need to use metamaterials for cloak designs that are inherently narrowband, dispersive and highly sensitive to polarization and incident angle. A new interpretation of the TO algorithm is presented and is employed for the design of radar cross section-reducing absorbers for arbitrary targets, and not just for canonical shapes, e.g., cylinders. The topic of performance enhancement of the absorbers by using graphene materials and embedded frequency structure surfaces is briefly mentioned. The design procedure for planar absorbing covers is presented and their performance as wrappers of general objects is discussed. A number of test cases are included as examples to illustrate the application of the proposed design methodology, which is a modification of the classical TO paradigm.

• Journal of Information Processing Systems
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• 제15권2호
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• pp.374-385
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• 2019

This paper presents an optimal implementation of a Daubechies-based pipelined discrete wavelet packet transform (DWPT) processor using finite impulse response (FIR) filter banks. The feed-forward pipelined (FFP) architecture is exploited for implementation of the DWPT on the field-programmable gate array (FPGA). The proposed DWPT is based on an efficient transpose form structure, thereby reducing its computational complexity by half of the system. Moreover, the efficiency of the design is further improved by using a canonical-signed digit-based binary expression (CSDBE) and advanced functional sharing (AFS) methods. In this work, the AFS technique is proposed to optimize the convolution of FIR filter banks for DWPT decomposition, which reduces the hardware resource utilization by not requiring any embedded digital signal processing (DSP) blocks. The proposed AFS and CSDBE-based DWPT system is embedded on the Virtex-7 FPGA board for testing. The proposed design is implemented as an intellectual property (IP) logic core that can easily be integrated into DSP systems for sub-band analysis. The achieved results conclude that the proposed method is very efficient in improving hardware resource utilization while maintaining accuracy of the result of DWPT.