• Food Science and Preservation
• /
• v.20 no.6
• /
• pp.886-893
• /
• 2013

Several indigenous sulfite-resistant yeasts were isolated at the microbial succession stage of yeast flora during spontaneous fermentation of Campbell Early grapes using a YPD plate that contained 200 mg/L or 500 mg/L potassium metabisulfite. When they were applied to the wine fermentation using the Campbell Early grape and apple juices, strains S13 and D8 showed strong alcohol fermentation and good flavor production. They were identified as Saccharomyces cerevisiae in the phylogenetic analysis based on their ITS 1-5.8S-ITS II DNA sequences. The two yeast strains grew to a high cell density in the YPD media supplemented with 40%(w/v) glucose. They also grew rapidly in the YPD media at $40^{\circ}C$. While strain S13 showed some differences in cell density at the two temperatures, no marked difference was observed during the culture of strain D8. The strains grew relatively well at pH 5.0 and 9.0 compared with pH 7.0, which was the optimum pH for their growth. Especially, strain S13 cultivated in the YPD media at pH 9.0 grew to 93% of the growth of strain D8, which was obtained at pH 7.0.

• Korean Journal of Agricultural Science
• /
• v.18 no.1
• /
• pp.1-9
• /
• 1991

This experiment was aimed to develop possible methods to control the maturity of grape berries through the application of exogenous plant growth substances and physical treatments such as defoliation or girdling. 1. Chlormequat and paclobutrazol increased anthocyanin but did not affect soluble solids contents and maturity. Girdling enhanced maturation and solids accumulation whereas defoliation delayed maturity. Solids content of berries in defoliation treatment did not reach to the level of other treatments even when fully ripened. 2. Ripening of grapes is greatly delayed for 20 to 30 days by the application of auxins (2,4-D and fenoprop) compared to the untreated control. Uneven ripening of berries in those clusters was observed when the concentration of auxin was over 50 ppm. Thus, about 30% of berries remained green until the normal berries were overripened. 3. Gibberellin did not affect the maturity of grape berries but maturity was greatly delayed when GA was applied with auxins. Also, uneven coloration between berries was observed such as in the application of auxin alone. 4. Ethephon application combinded with calcium at veraison showed no effect on berry ripening but increased anthocaynin contents. It can be concluded that harvest time of 'Campbell Early' grapes can be effectively extended by delaying the maturity through the application of auxin.

• Korean Journal of Organic Agriculture
• /
• v.24 no.4
• /
• pp.773-786
• /
• 2016

Grape cultivar "Campbell Early" account for 70% of table grape in Korea and Leaf Spot Disease caused by Pseudocercospora vitis is one of a major disease in greenhouse and field grown area during late summer season in both of organic and conventional grape farm. Leaf spot disease can cause lowing of sugar content in fruit and vine growth and very difficult to control especially in organic field. Photosynthesis ability and chemical components are compared between leaf spot disease infected leaves with degree of necrotic area. With increase of disease necrotic area, $CO_2$ differential value, water use efficiency and $CO_2$ assimilation and respiration ratio are decreased proportionally and on the other hand, stomatal conductance value is not affected by disease necrotic area. Chlorophyll contents are also decreased by 50% in heavily infected leaves and imply decrease of chlorophyll contents is a major source of photosynthesis ability decline. With increase of disease necrotic area in leaves, total nitrogen and phosphate contents are decreased and on the other side, total carbon, potassium, calcium and magnesium contents are increased. From this research, we can infer that not only chemical control program is important in control of leaf spot disease but also fertilizing program is significant especially in organic agronomical control of fungal disease in grape cultivar "Campbell Early".

• Research in Plant Disease
• /
• v.20 no.4
• /
• pp.275-282
• /
• 2014

The occurrence of post-harvest diseases and their pathogens in 'Campbell Early' which is the most produced grape in Korea was investigated. The 'Campbell Early' grapes produced in 3 main grape-producing areas were stored in a cold room ($0-4^{\circ}C$) for 2 weeks then at room temperature for 4 weeks prior to investigation. The major post-harvest diseases occurred were gray mold, blue mold, ripe rot, new decay 1, and new decay 2. Pathogens isolated from the symptoms were identified as Botrytis cinerea for gray mold, Penicillium sclerotiorum for blue mold and Collectrichum acutatum for ripe rot. Pathogens for new decay 1 and new decay 2 were not identified yet. Incidences of new decay 1 and new decay 2 were much higher than the other 3 decays in all grapes produced from 3 areas. Gray mold and blue mold occurred at much lower frequencies than these two decays, and ripe rot occurred least.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.42 no.2
• /
• pp.168-174
• /
• 2013

We investigated the physiological activities of extracts from Campbell Early and Muscat Bailey A (MBA) grapevine leaves. Total phenol and flavonoid contents were highest in ethanol extracts from MBA grapevine leaves compared to extracts from Campbell Early grapevine leaves. Specific polyphenols higher in ethanol extracts from MBA grapevine leaves include gallic acid, epicatechin, caffeic acid, naringin, and resveratrol. Resveratrol content from MBA grapevine leaves increased when extracted for more than two hours in ethanol and water. The hydroxyl radical scavenging ability of ethanol extracts was higher than the water extract from both strains of grapevine leaves. DPPH and total antioxidants were highest in ethanol extracts from MBA grapevine leaves among the other extracts. Therefore, these results suggest that ethanol extracts from MBA grapevine leaves are a highly valuable resource for the development of natural functional foods.

• Korean Journal of Organic Agriculture
• /
• v.23 no.4
• /
• pp.897-909
• /
• 2015

This study was conducted to evaluate the effect of weed control on disease incidence, plant growth and fruit quality, and to provide effective method to farmers at the vineyard. At the raincoat greenhouse cultivating 'Campbell Early', several weed controlling methods were compared to the control. Mean temperature of plots between soil covering (C.B., W.F., S.N., P.F. and R.H.) and non soil covering (M.W.2, M.W.3 and U.T.) were similar with $26.2^{\circ}C$ (July) and $25.8^{\circ}C$ (Aug.), but humidity of soil covering was sharply decreased in late-July under 40%. Soil water were all higher in soil covering plots than that of non-soil covering, however, soil temperature was recorded lower in soil covering plots. Disease incidence of Peudocercospora vitis according to soil coverings had effect on black plastic film mulching by decreasing grapevine leaf spot to 85% in July, 69% in August, compared to the control, with showing the smallest lesion incidence in FMB. Among growth characters, internode length and internode width of black plastic film mulching was higher than that of the control. SPAD value of black plastic film mulching was recorded the highest. It was not significant among the treatments, but brix was recorded highly in black plastic film mulching with $14{\sim}15^{\circ}Brix$.

• Journal of Plant Biotechnology
• /
• v.29 no.2
• /
• pp.105-110
• /
• 2002

Effects of difference between day and night temperatures (DIF) and cytokinins on in vitro growth of 'Campbell Early' grape plantlets were investigated, Shoot growth was suppressed by -7DIF (18/$25^{\circ}C$). Effects of BA and TDZ were not affected by DIF. 0DIF (25/$25^{\circ}C$) enhanced the growth with increasing cytokinin level. Kinetin at 10 $\mu$M was the most effective for shoot growth with 87 mm among cytokinins used except for control. Zeatin and kinetin each at 5 $\mu$M significantly increased shoot growth to respective 162 and 110 mm. In 0DIF, two-fold increase of 162 mm was obtained by 5 $\mu$M zeatin. In control, no further shoot proliferation was observed regardless of DIF. In 0 and ＋7DIF (25/18$^{\circ}C$), 4 shoots were observed with 10 $\mu$M BA. Zeatin, 2iP and kinetin resulted in poor shoot proliferation while BA at 10 $\mu$M resulted in profuse branching in all DIFs.

• Korean Journal of Food Science and Technology
• /
• v.44 no.3
• /
• pp.280-286
• /
• 2012

The aim of this study was to describe the effects of genetic factors on the chemical composition or metabolites of grapes harvested within the same region. Grapes were separated into pulp, skin, and seed, and physicochemical characteristics were compared among seven grape varieties. The sugar concentrations of the grape musts ranged from $15.17-20.93^{\circ}Bx$ with Seibel variety being highest at $20.93^{\circ}Bx$. pH ranges of grape musts were 3.46-4.02 and total acidity was highest with 1.05 in Steuben variety. Tartaric acid content was highest with 146.68, 500.10 mg/L in pulp and skin extract of Merlot variety. Malic acid content was highest in pulp extract of Seibel variety (1127.14 mg/L) and skin extract of Chardonnay variety (1720.06 mg/L). K content was highest with 379.13 and 828.01 mg/L in pulp and skin extract of Chardonnay variety. Ca content was highest in pulp extract of Kyoho variety (6.98 mg/L) and skin extract of Campbell Early variety (12.26 mg/L).

• Korean Journal of Food Science and Technology
• /
• v.34 no.2
• /
• pp.151-156
• /
• 2002

Five kinds of red wine from three different grape varieties, Gerbong (G), Campbell (C), Moru (M), Gerbong+Moru (70:30, GM) and Gerbong+Campbell (70:30, GC), were prepared. Their chemical and microbiological changes were monitored during alcohol fermentation and aging. The changes of pH, brix and total acidity were $3.2{\sim}3.6,\;17.9{\sim}6.0$ and $2.4{\sim}4.6\;g/L$, respectively. The viable cell numbers of initial, stationary and death phases were $6.0{\times}10^6,\;1.0{\times}10^8$ and $7.0{\times}10^5\;cfu/mL$ during alcohol fermentation. The sugar fermentability, glucose and fructose contents were greatly decreased less than 0.2 g/L, and the final contents of ethanol and sulfur dioxide were $11.4{\sim}12.3%$ and $40{\sim}62\;mg/L$, respectively. The conversion ratios of malic acid to lactic acid were 23% (G), 67% (M), 28% (C), 33% (GM) and 39% (GC). The chemical characteristics of five red wine were significantly different in pH, total acidity, sulfur dioxide and lactic acid contents (p<0.05).

• Food Science and Preservation
• /
• v.18 no.5
• /
• pp.811-816
• /
• 2011

Cryoextraction (a freeze concentration using an instrument) can increase the sugar concentration in grape juice by reducing its water content, similar to the natural freezing of grapes for natural ice wine. In this study, fermentation of freeze-concentrated Campbell Early grape (Vitis labruscana) juice to 36 $^{\circ}Bx$ was carried out using Saccharomyces cerevisiae strains D8 and S13 isolated from Korean grapes. During the fermentation, strains S13 and D8 showed rapid sugar reduction and alcohol production compared with S. cerevisiae Fermivin$^{(R)}$ used as a control. After nine-day fermentation, the residual sugar contents were lower in W13(9.77%) and D8 wine(9.07) than that in Fermivin$^{(R)}$ wine(14.0%). Total acid content was high in the D8>S13>Fermivin$^{(R)}$ wine, in that order. The acetaldehyde content was highest in the D8 wine and lowest in the Fermivin$^{(R)}$ wine, among the three. The methanol content was slightly higher in the S13 and D8 wines than in the Fermivin$^{(R)}$ wine. In the sensory evaluation, the S13 wine exhibited the highest score in flavor and taste among the three wines. Both the two S13 and D8 wines exhibited higher scores than Fermivin$^{(R)}$ wine in overall preference.