• Korean Journal of Environment and Ecology
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• v.28 no.2
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• pp.205-214
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• 2014

The purpose of this study is to suggest vegetation management method as the cultural landscape forest of Dongbaek Island which is the district monument. The study area was $20,000m^2$ around the peak area in management as the nature sabbatical area. Vegetation structure type was classified with the criteria of topography, vegetation, management and the management plan was derived from the vegetation structure analysis according to the types. Vegetation structure types were Management-Camellia japonica, Non-management-Eurya japonica, Non-management-Rugged area-Eurya japonica. As the result of vegetation structure, Pinus thunbergii dominated in canopy layer and Camellia japonica and Eurya japonica dominated in Type I and in Type II and III, respectively. Especially, Machilus thunbergii as the climax species in the warm temperate forest were distributed centering shrubs, and as the result of distribution of diameter of breast height, middle size of Celtis sinensis and Machilus thunbergii were distributed in type I, II. Machilus thunbergii were distributed in range of 4 to 44 individuals through the all types. Mean age of canopy layer was 66 year-old and sub-canopy layer was 22.9 year-old. Shanon's species diversity was analysed from 0.5472 to 0.8646. As the vegetation management direction of Dongbaek island, managed Camellia japonica forest was suggested to maintain the regular management and non-managed Eurya japonica forest was required to remove the Eurya japonica and plant the Camellia japonica. In case of non managed Eurya japonica forest in rugged area, vegetation succession was required to laurel forest.

• Journal of Dairy Science and Biotechnology
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• v.34 no.2
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• pp.69-74
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• 2016

The main constituent of tea catechins, EGCG [(-)-Epigallocatechin-3-gallate], could inhibit the growth of various microorganisms and differently affect gram-positive and gram-negative bacteria. Antimicrobial activity of EGCG, a compound from green tea (Camellia sinensis) extract, against Cronobacter spp. and Salmonella spp. was studied to evaluate the possibility of using EGCG as a natural food additive in various dairy products. In pure TSB culture, the growth of Cronobacter spp. was suppressed below the detection limit (1 log CFU/mL) depending on EGCG concentration ($600{\sim}800{\mu}g/mL$), after 5~16 days at $4^{\circ}C$. Similarly, the growth of Salmonella spp. was suppressed below the detection limit (1 log CFU/mL) depending on EGCG concentration ($400{\sim}800{\mu}g/mL$), after 5~16 days at $4^{\circ}C$. Therefore, these results suggest that EGCG could be used as an effective additive to inhibit the growth of Cronobacter spp. and Salmonella spp. in various dairy products, such as yoghurt, cheese, dried infant powder, and so on.

• Journal of Food Hygiene and Safety
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• v.31 no.4
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• pp.278-285
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• 2016

This study used response surface methodology (RSM) in an effort to optimize the ultrasoundassisted extraction condition of Camellia sinensis L. and Artemisia argyi mixture in order to increase extraction yield in the extract. The effects of three independent variables, $X_1$ (Mixture ratio, 60-80%), $X_2$ (Ratio of water to raw material, 20~100 mL/g), and $X_3$ (Extraction time, 25-145 min), were investigated at three levels using Box-Behnken design (BBD) to obtain the highest extraction efficiency. Y (Extraction yield) was chosen as dependent variable. Our result showed that the coefficient of determination ($R^2$) of the model was 0.9747, with significant at the level of p < 0.002. Furthermore, the predicted values of each variable were similar to the actual values. The optimum extraction conditions were as follows: mixture ratio of 85.86%, ratio of water to raw material of 92.73 mL/g, and extraction time of 56.52 min. At these conditions, predicted extraction yield was 30.03%. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing extraction conditions of Camellia sinensis L. and Artemisia argyi mixture.

• Journal of the East Asian Society of Dietary Life
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• v.17 no.6
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• pp.911-918
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• 2007

Korean tea leaves (Camellia sinensis var. sinensis,), which were harvested in April, were fermented for various times, and then the changes in free amino acids (glutamic acid, GABA, arginine, theanine), pH, color values, and sensory qualities were evaluated. The pH in every sample became lower as fermentation time increased. Lightness also decreased as tea fermentation increased. Furthermore, glutamic acid, GABA, and arginine contents increased, while theanine content decreased, where it presented as 69% of the non-fermented tea leaves. GABA, a hypotensive compound, increased in the fermented tea leaves according to the degree of fermentation and hand rolling times. In terms of the theanine relaxation effect as well as taste, a shorter fermentation time is required, and for a hypotensive effect of GABA, fermentation needs to be increased. For the sensory evaluation, the preferred blend of taste and flavor was highest in the S8 tea sample.

• Korean Journal of Environment and Ecology
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• v.33 no.5
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• pp.539-551
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• 2019

The purpose of this study is to understand the vegetation structure and ecological characteristics of the coastal forest Pinus thunbergii community in Busan Metropolitan City (BMC) and to establish reference information for the management of the coastal forest in BMC in the future. We set up 97 plots with an area of $100m^2$ each for the analysis and investigation of the vegetation characteristics. The analysis using the TWINSPAN and DCA techniques found seven community groups: Pinus thunbergii-Quercus aliena community, Pinus thunbergii-Eurya japonica(1) community, Pinus thunbergii-Eurya japonica(2) community, Pinus thunbergii-Quercus serrata community, Pinus thunbergii-Camellia japonica(1) community, Pinus thunbergii-Camellia japonica(2) community, and Pinus thunbergii-Eurya japonica-Camellia japonica community. According to the analysis of vegetation structure, Pinus thunbergii was a main dominant species at the canopy layer in all sites while Eurya japonica and Camellia japonica were dominant species at the understory layer. Pinus thunbergii-Quercus serrata community is predicted in the forest succession because of the competition between Pinus thunbergii and Quercus serrata in the canopy layer and the understory layer. As such, it is necessary to observe changes by continually monitoring this community. Tree species with strong salinity tolerance, including Pinus thunbergii, have formed community groups because of the environmental characteristics of coastal forests, strong with salinity tolerance species are forming community groups. Therefore, all community groups except for the Pinus thunbergii-Quercus serrata community will maintain the current vegetation structure unless drastic environmental changes occur.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.845-855
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• 2021

In this study, Camellia sinensis Leaf cultivated in Boseong was extracted by vacuum distillation and hydrothermal extraction methods, and we performed in vitro experiments such as antioxidant, anti-wrinkle, whitening, and antibacterial activity test and HPLC analysis. Wrinkle improvement (elastase inhibition assay) and whitening effect (mushroom tyrosinase inhibition assay) of Boseong vacuum distilled extract have excellent results and IC50 values of vacuum distillation extract was measured at a lower concentration than that of the hydrothermal extract. The antioxidant activity of the Boseong hydrothermal extract was excellently measured in DPPH radical and ABTS+ radical scavenging activity, SOD-like activity, and total polyphenol content. In the antibacterial experiment, the Boseong hydrothermal extract formed a clear zone for the Staphylococcus aureus and Cutibacterium acnes strain. As a result of HPLC analysis, EGCG and caffeine in the hydrothermal extract were 40.29 mg/g, 9.94 mg/g respectively, and caffeine in the vacuum distilled extract was 3.40 mg/g. Therefore, we examined that Boseong Camellia sinensis vacuum distilled extract has excellent anti-wrinkle and whitening effect, and its hydrothermal extract has good antioxidant and antibacterial efficacy.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.503-511
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• 2023

The aim of this study was to investigate in vitro antioxidant activities of defatted Camellia japonica L. seeds (DCJS). The DCJS were extracted using ethanol and then fractionated with butanol (BuOH), ethyl acetate (EtOAc), chloroform, and hexane. To evaluate antioxidant activity of extract and fractions from DCJS, we investigated free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), hydroxyl radical (^•OH), and superoxide anion (O₂^-) radicals. The five extract and fractions of DCJS dose-dependently increased DPPH, ABTS⁺ and O₂^- radical scavenging activities. The BuOH fraction of DCJS showed the highest free radical scavenging activities among other extract and fractions. The contents of total polyphenol and flavonoid in BuOH fraction of DCJS were 23.26 mg GAE/g and 32.39 mg QE/g, respectively. The polyphenol and flavonoids contents of BuOH fraction has highest than other extract and fractions. In addition, BuOH and EtOAc fraction of DCJS contained 102.37 and 165.05 ㎍/g of camelliaside B, respectively. Therefore, DCJS has higher antioxidant activity and may be useful as a natural antioxidant material.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.118-118
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• 2005

• Bulletin of Food Technology
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• v.18 no.4
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• pp.128-139
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• 2005

식용유지나 지방질을 함유하고 있는 유지식품은 저장 및 가공 중에 산화가 일어나 식품에 바람직하지 않은 결과를 나타낸다. 그러므로 영양학적, 기술적 과정에서 볼 때 저해제 첨가에 의한 산화억제가 바람직하다. 본 논문은 몇가지 지질 시스템과 식품 중에서 차 폴리페놀의 항산화 활성 메카니즘, 항산화제 작용부위 그리고 항산화 물질로서의 가능성에 대해 살펴보았다.

• 한국약용작물학회:학술대회논문집
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• 2007.11a
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• pp.303.1-303.1
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• 2007