• Title/Summary/Keyword: Calcium

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Fluoride and Calcium in Tea Leaves

  • Takiyama, Kazuyoshi;Ishii, Yuuko
    • Analytical Science and Technology
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    • v.8 no.4
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    • pp.877-880
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    • 1995
  • The determination of fluoride and the nutritious calcium in infusion of teas are explained. Tea leaves were pulverized and were immersed in boiling water. The solution was filtered and fluoride, calcium and oxalic acid were determined by the ion chromatography. The quantities of fluoride, calcium and oxalate ions extracted from 100 g of tea leaves were calculated. Tea leaves were also immersed in 0.5 M hydrochloric acid and extracted oxalate and calcium ions were analyzed. The free oxalic acid and calcium were extracted in boiling water and the total ones were extracted in hydrochloric acid. The quantity of calcium oxalate was calculated from the total and the free oxalic acids. The free calcium was estimated to be nutritious.

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Serum Total Calcium, Ionized Calcium Ion and Lipid Compositions in Hypertensive Koreans (정상인과 고혈압 환자의 식이 Calcium 섭취빈도와 혈청의 Calcium 수준과 지질조성의 비교)

  • 박광희
    • Journal of Nutrition and Health
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    • v.22 no.6
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    • pp.476-484
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    • 1989
  • An epidemiological survey was made on dietary calcium intake, serum total calcium and ionized calcium ion, and serum lipid compositions. Compared with 120 normotensive controls, 101 hypertensive subjects were significantly higher in the levels of serum cholesterol and triglyceride, but lower in HDL-cholesterol and higher in the ratio of total choesterol/HDL-chol Hypertensive subjects were also significantly lower in the relative amount of fatty acids C18:2 but higher in those of C20:0, C20:2 and total amount of saturated fatty acids(longer than C14:0) than control serum. Patients were significantly lower in serum total calcium and ionized calcium ion concentrations and significantly less calcium ingestion from milk and dairy produces by feeding frequency test.

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Peptidoglycan Induces the Production of Interleukin-8 via Calcium Signaling in Human Gingival Epithelium

  • Son, Aran;Shin, Dong Min;Hong, Jeong Hee
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.1
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    • pp.51-57
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    • 2015
  • The etiology of periodontal disease is multifactorial. Exogenous stimuli such as bacterial pathogens can interact with toll-like receptors to activate intracellular calcium signaling in gingival epithelium and other tissues. The triggering of calcium signaling induces the secretion of pro-inflammatory cytokines such as interleukin-8 as part of the inflammatory response; however, the exact mechanism of calcium signaling induced by bacterial toxins when gingival epithelial cells are exposed to pathogens is unclear. Here, we investigate calcium signaling induced by bacteria and expression of inflammatory cytokines in human gingival epithelial cells. We found that peptidoglycan, a constituent of grampositive bacteria and an agonist of toll-like receptor 2, increases intracellular calcium in a concentration-dependent manner. Peptidoglycan-induced calcium signaling was abolished by treatment with blockers of phospholipase C (U73122), inositol 1,4,5-trisphosphate receptors, indicating the release of calcium from intracellular calcium stores. Peptidoglycan-mediated interleukin-8 expression was blocked by U73122 and 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis (acetoxymethyl ester). Moreover, interleukin-8 expression was induced by thapsigargin, a selective inhibitor of the sarco/endoplasmic reticulum calcium ATPase, when thapsigargin was treated alone or co-treated with peptidoglycan. These results suggest that the gram-positive bacterial toxin peptidoglycan induces calcium signaling via the phospholipase C/inositol 1,4,5-trisphosphate pathway, and that increased interleukin-8 expression is mediated by intracellular calcium levels in human gingival epithelial cells.

Women's Calcium Intake during Late Pregnancy and Breastfeeding (일 지역 여성의 임신기와 수유기 칼슘섭취 양상)

  • Ahn, Suk-Hee;Kim, Jin-Hee
    • Journal of muscle and joint health
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    • v.18 no.1
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    • pp.63-72
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    • 2011
  • Purpose: The purpose of the study was to identify the amount of calcium intake during late pregnancy and breastfeeding and compare the differences in the amount of calcium by subjects' characteristics. Methods: With a descriptive survey design, 121 Korean breastfeeding women were recruited in a community setting. The list of foods and drinks with calcium extracted from the study of Song and So (2007) was used to measure the amount of calcium intake during pregnancy and lactation. The subjects' characteristics were collected to identify differences in the amount of calcium intake. Results: Mean age of the women was 31 years old. Amount of calcium intake was 568mg per day during late pregnancy and 431mg per day during breastfeeding. These amounts were quite lower than a recommended dose that adult women should consume a daily minimum of 1,000mg of calcium. Women who had higher household income and attending experience for nutritional education took more calcium during breastfeeding and late pregnancy, respectively. Conclusion: More than a half of the breast-feeding women do not consume the recommended dose of calcium. Education for proper calcium consumption during pregnancy and lactation should be an additional subject in the prenatal and breastfeeding education program for women's bone health.

The Effects of Calcium Nutrition on the Activities of Lactate Dehydrogenase, Alcohol Dehydrogenase and Other Enzymes in Melon (Cucumis melo L.) Seedlings Subjected to Flooding

  • Lee, Chang-Hee;Park, Man;Kang, Sang-Jae
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.1
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    • pp.36-43
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    • 2016
  • With transient flooding followed by poor or slow drainage plant roots may become reduction conditions because the root zone was fully filled with water. This study was examined the effects of calcium treatment in the early growth stage on biochemical changes in leaves and roots of melon (Cucumis melo L.) seedlings kept under flooding condition for 72 h. The activities of lactate dehydrogenase more gradually enhanced in the roots than those of leaves of melon seedlings treated with calcium. The activities of alcohol dehydrogenase associated with alcohol fermentation under low oxygen conditions continuously increased in the leaves and roots of seedlings untreated with calcium under flooding at least 72 h but those was constant within at least 12 h in treated with calcium. These results showed that calcium supplying in the early growth stage mitigated alcohol fermentation of melon seedlings kept under flooding condition for 72 h. Activities of nitrate reductase and acid phosphatase in the leaves and roots of seedlings in treated with calcium somewhat higher than those of non-treated with calcium. The activities of sucrose phosphate synthase and fructose-1,6-bisphosphatase of leaves of seedlings in treated with calcium more higher than those of non-treated with calcium. These results indicated that calcium nutrition mitigate the reduction of activities of some enzymes of melon seedling kept under flooding condition for 72 h.

Differentiation of Canine Calcium Oxalate and Canine Struvite Stones using Computed Tomography (개에서 전산화단층촬영을 이용한 Calcium Oxalate결석과 Struvite결석의 감별)

  • Yoon, Young-Min;Lee, Hee-Chun
    • Journal of Veterinary Clinics
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    • v.32 no.1
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    • pp.69-72
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    • 2015
  • This study was performed to differentiate calcium oxalate and struvite canine urinary stones using computed tomography. A total of 38 urinary stones (8 calcium oxalate and 30 struvite) were scanned using a computed tomography scanner. These urinary stones (10-15 mm diameter) extracted surgically without fragmentation were obtained from the different individual patients. The stone's Hounsfield units(HU) values, heterogenicity, and roughness of surface were evaluated to differentiate calcium oxalate and struvite. The HU values of calcium oxalate were significantly higher than those of struvite. A receiver operator characteristic (ROC) curve revealed 1272 as the best threshold value to distinguish calcium oxalate from struvite (ROC curve AUC 0.87, p < 0.0014). The heterogenicity of calcium oxalate and struvite significantly differed on bone and dental window setting (p < 0.0001). There was no significant difference between calcium oxalate and struvite in roughness of surface. On computed tomographic images, bone and dental windows setting were useful for evaluation of heterogenicity between calcium oxalate and struvite. The HU value and heterogenicity are highly promising factor that can distinguish calcium oxalate and struvite with reasonable accuracy.

High concentration of calcium represses osteoblast differentiation in C2C12 cells

  • Lee, Ye Jin;Han, Younho
    • International Journal of Oral Biology
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    • v.45 no.4
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    • pp.162-168
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    • 2020
  • Calcium is the most abundant stored mineral in the human body and is especially vital for bone health; thus, calcium deficiency can cause bone-related diseases, such as osteopenia and osteoporosis. However, a high concentration of serum calcium, which is commonly known as hypercalcemia, can also lead to weakened bones and, in severe cases, osteosarcoma. Therefore, it is necessary to maintain the concentration of calcium that is appropriate for bone biology. In the present study, we aimed to elucidate the effects of high concentration of calcium, approximately 2 folds the normal calcium level, on osteoblast differentiation. The CaCl2 treatment showed dose-dependent suppression of the alkaline phosphatase activity and mineralized nodule formation. Calcium showed cytotoxicity at an extremely high concentration, but a moderately high concentration of calcium that results in inhibitory effects to osteoblast differentiation showed no signs of cytotoxicity. We also confirmed that the CaCl2 treatment repressed the mRNA expression and protein abundance of various osteogenic genes and transcriptional factors. Considered together, these results indicate that a high concentration of calcium negatively regulates the osteoblast differentiation of C2C12 cells.

Effects of Organic Acids on Solubility of Calcium (칼슘용해에 미치는 유기산의 영향)

  • Jang, Se-Young;Park, Nan-Young;Jeong, Yong-Jin
    • Korean Journal of Food Preservation
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    • v.12 no.5
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    • pp.501-506
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    • 2005
  • This study was conducted to investigate the effect of organic acids on solubility of calcium. As a results, acetic and lactic acid showed the most excellent solubility of calcium. Calcium solubility was increased at initial total acid (4%) in citric acid but calcium was insoluble in tartaric acid. After solving, pH and residuals were decreased where as total acidity and calcium content were increased as increment of initial acidity of acetic and lactic acid. Calcium content in seaweed calcium and calcium carbonate were higher than that of nano calcium. Solubility of calcium was more conspicuous at lactic acid than acetic acid.

Haloperidol Induces Calcium Ion Influx Via L-Type Calcium Channels in Hippocampal HN33 Cells and Renders the Neurons More Susceptible to Oxidative Stress

  • Kim, Hyeon Soo;Yumkham, Sanatombi;Choi, Jang Hyun;Kim, Eung-Kyun;Kim, Yong Sik;Ryu, Sung Ho;Suh, Pann-Ghill
    • Molecules and Cells
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    • v.22 no.1
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    • pp.51-57
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    • 2006
  • Haloperidol is a classical neuroleptic drug that is still in clinical use and can lead to abnormal motor activity following repeated administration. However, there is little knowledge of how it triggers neuronal impairment. In this study, we report that it induced calcium ion influx via L-type calcium channels and that the elevation of calcium ions induced by haloperidol appeared to render hippocampal cells more susceptible to oxidative stress. Indeed, the level of cytotoxic reactive oxygen species (ROS) and the expression of pro-apoptotic Bax increased in response to oxidative stress in haloperidol-treated cells, and these effects were inhibited by verapamil, a specific L-type calcium channel blocker, but not by the T-type calcium channel blocker, mibefradil. These findings indicate that haloperidol induces calcium ion influx via L-type calcium channels and that this calcium influx influences neuronal fate.

Effect of Extracellular Calcium on Vitellogenin Production in the Culture of Hepatocytes in the Rainbow Trout, Oncorhynchus mykiss

  • Yeo In-Kyu;Mugiya Yasuo;Chang Young Jin;Hur Sung Bum;Yoo Sung Kyu
    • Fisheries and Aquatic Sciences
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    • v.1 no.1
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    • pp.24-29
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    • 1998
  • Effect of extracellular calcium in vitellogenin (VTG) production in response to estradiol-17 $\beta$ $(E_2,\;2\times10^{-6}M)$ was examined in primary hepatocyte culture of rainbow trout, Onchorhynchus mykiss. Total calcium in estrogenized sera significantly increased, compared with the control, while diffusible calcium was insignificant. However, diffusible calcium in the incubation medium with $E_2$ was significantly reduced, compared with the control. The uptake of extracellular calcium by cultured hepatocytes signifIcantly increased 90 min after $E_2$ addition. Moreover, the accumulation of intracellular calcium increased in the cultures with $E_2$, regardless of the calcium concentrations in the incubation media. In addition, $E_2-primed $ VTG production was significantly decreased by withdrawal of E_2$ from the incubation medium. Moreover, VTG production by $E_2-primed$ hepatocytes was reduced by removing calcium from the incubation medium with or without $E_2$. These results suggest that the entry of extracellular calcium into the cytoplasm is an important step for VTG production in primary hepatocyte cultures in rainbow trout.

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