• Title/Summary/Keyword: Calcium

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Gender-dependent difference in serum paraoxonase 1 levels of Hanwoo, Korean native cattle, and a positive association with meat quality

  • Park, Jihyun;Kim, Jiwoo;Hwang, Sungwon;Chung, Ki Young;Choi, Inho;Choi, Chang Bon;Kim, Jihoe
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.3
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    • pp.437-441
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    • 2019
  • Objective: Paraoxonase 1 (PON1), a calcium-dependent serum enzyme, has been shown to be involved in lipid metabolism. In this study, we examined the putative correlation of the serum PON1 level of Hanwoo, Korean native cattle, with gender and meat quality grade. Methods: PON1 levels were estimated by determining the arylesterase and paraoxonase activities (AE and PO, respectively) in serum samples from Hanwoo individuals (n = 56). Serum PON1 levels were analyzed in different gender groups (female [n = 21], castrated male [n = 17], and male [n = 18]), and meat quality grades (${\geq}1$ [n = 23], 2 [n = 21], and 3 [n = 12]). Results: Serum PON1 levels were similar in female ($AE=120{\pm}55U/mL$, $PO=84{\pm}43mU/mL$) and castrated male ($123{\pm}44U/mL$, $PO=89{\pm}30mU/mL$), while male showed a significantly lower level ($AE=65{\pm}43U/mL$, $PO=44{\pm}34mU/mL$). Furthermore, analysis of serum PON1 levels in three different grades of meat quality showed similar levels in the grades ${\geq}1$ ($AE=118{\pm}49U/mL$, $PO=84{\pm}37mU/mL$) and 2 ($AE=116{\pm}54U/mL$, $PO=82{\pm}43mU/mL$), while the level was significantly lower in the grade 3 ($AE=58{\pm}35U/mL$, $PO=39{\pm}27mU/mL$) of lower meat quality. Conclusion: We discovered the gender-dependent differences in serum PON1 levels of Hanwoo and a positive association of the serum PON1 level with meat quality. Results in this study suggest that PON1 would be a useful serum marker for preliminary screening of Hanwoo individuals with high-quality meat and applicable for genetic improvement.

Reference intervals for blood metabolic profiles of Holstein cows in Korea (국내 젖소의 혈액 대사인자 프로파일 분석)

  • Jung, Suk-Han;Jung, Young-Hun;Choe, Changyong;Do, Yoon Jung;Cho, Ara;Oh, Sang-Ik;Kim, Eunju;Ha, Seungmin;Jeong, Ha Yeon;Yoo, Jae Gyu;Kim, Suhee
    • Korean Journal of Veterinary Service
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    • v.42 no.2
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    • pp.121-126
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    • 2019
  • Metabolic profile test is used to evaluate nutritional imbalance and metabolic disease in dairy cows. The reference intervals of metabolic parameters may change according to nation, region, decades, and maintenance system. Despite the need to be periodically updated for the reference intervals of metabolic parameters, it has rarely been investigated in Korea. Therefore, this aim of study was to provide the reference intervals of metabolic parameters using dairy cows surveyed in Korea during recent years. A metabolic profile test was conducted for 2,976 clinically healthy dairy cows in Korea. Blood samples were collected for the analysis of serum metabolites. This study provided reference intervals of thirteen metabolic parameters (${\beta}$-hydroxybutyrate [${\beta}-HB$], non-esterified fatty acids [NEFA], glucose, total cholesterol [T-COL], total protein, albumin, globulin, blood urea nitrogen [BUN], aspartate aminotransferase [AST], gamma-glutamyl transferase [GGT], calcium, phosphorus, and magnesium). BUN and AST values of the current study were higher than those of previous studies. In the present study, the other metabolic parameters showed low or similar value compared to previous results. Moreover, ${\beta}-HB$, NEFA, T-COL, ALB, BUN, AST, and GGT values were affected by lactation period. This study provided information on the reference intervals of metabolites in healthy dairy cows in Korea. The reference intervals from the present study would be useful in managing and diagnosing disease of dairy cows. However, careful attention should be given in interpreting disease condition for metabolites affected by lactation.

Graded levels of phytase on performance, bone mineralization and carcass traits of broiler fed reduced dicalcium phosphate

  • de Freitas, Henrique Barbosa;de Souza Nascimento, Karina Marcia Ribeiro;Kiefer, Charles;Gomes, Gilson Alexandre;Santos, Tiago Tedeschi dos;Garcia, Elis Regina Moraes;da Silva, Thiago Rodrigues;Paiva, Luanna Lopes;Berno, Patricia Rodrigues
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.5
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    • pp.691-700
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    • 2019
  • Objective: This study was conducted to assess increasing doses of phytase added to broiler diets formulated with different levels of available phosphorus (avP), calcium (Ca), and sodium (Na), and the respective effects on performance parameters, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs. Methods: Three different matrices were assumed for phytase with the following nutritional values: matrix A (MT A): 0.165% Ca, 0.150% avP, and 0.035% Na; matrix B (MT B): 0.215% Ca, 0.195% avP, and 0.045% Na; matrix C (MT C): 0.245% Ca, 0.225% avP, and 0.053% Na. There were six different diets: No phytase (formulated to meet the nutritional requirements); phytase 500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT A; phytase 1,500 FTU/kg+MT A; phytase 1,000 FTU/kg+MT B and phytase 1,500 FTU/kg+MT C. Results: There was no significant phytase influence on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of the organ throughout the study period, however, it was possible to observe a tendency of improvement in body weight corrected feed conversion for broilers fed the phytase 1,500+MT C diet, where potentially these birds were more efficient on utilize phytic phosphorus and other nutrients bounded to phytate molecule, translating into improvement in performance, and there was also a non significant numerical improvement in body weight corrected feed conversion of broilers fed this diet. Conclusion: Broilers fed with diets formulated with different levels of avP, Ca, and Na and increasing doses of phytase have shown no change on performance, quantitative carcass characteristics, ash and phosphorus deposition in tibia and weight of organs.

A Study on the Characteristics of Clinker and Cement as Chlorine Content (염소 함량에 따른 클링커 및 시멘트의 물성에 관한 연구)

  • Lee, Young-Jun;Kim, Nam-Il;Cho, Jeong-Hoon;Seo, Sung-Kwan;Chu, Yong-Sik
    • Resources Recycling
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    • v.30 no.5
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    • pp.10-16
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    • 2021
  • In this study, a clinker was prepared using raw materials with CaCl2. The characteristics of the chlorine-added clinker and cement were analyzed. The clinker modulus were set to Lime Saturation Factor (LSF) 92, Silica modulus (SM) 2.5, and Iron Modulus (IM) 1.5. The physical properties of cement using the chlorine-containing clinker were characterized. As the chlorine content increased, the free-CaO content in the clinker decreased, and that in the 2000 ppm clinker was reduced by approximately 40% compared to that in the 0 ppm clinker. There was an increase in the amount of chlormayenite, with a content of up to 3.4% present in the 2000 ppm clinker. The amounts of alite and belite also slightly increased. The compressive strength of mortar at 3 days and 7 days increased as the chlorine content increased. This trend was presumed to arise from the effect of hydration, which was promoted by the presence of chlorine. The compressive strength of 1000 ppm mortar increased by approximately 20% compared to that of 0 ppm mortar.

Determination of dietary Ca and P levels and their equivalence values of phytase and vitamin D3 for improved growth performance in weanling pigs

  • Ogola, Oketch Elijah;Choi, Jun Seung;Hong, Jun Seon;Kim, Yu Bin;Nawarathne, Shan Randima;Yu, Myunghwan;Heo, Jung Min
    • Korean Journal of Agricultural Science
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    • v.48 no.3
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    • pp.397-412
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    • 2021
  • In Experiment 1, the impact of using diets sufficient in P on the performance of weaned piglet growth with or without a super dose of phytase was investigated. One hundred and twelve piglets were randomized into four treatments with 7 replicates (4 piglets·cage-1). Bodyweight (BW), daily gain (ADG), daily intake (ADFI) and feed conversion ratios (FCR) were measured over three weeks. Treatments included a positive control (PC) with a Ca : P ratio of 0.80 : 0.50%. and the negative control (NC) had a Ca : P ratio of 0.60 : 0.50%. The PC plus phytase was denoted as PC+, and the NC with the phytase diet was designated as NC+. The PC diet was formulated to meet or exceed the NRC (2012) standards for pigs weighing 7 - 25 kgs. Better results were obtained with the PC diet (p > 0.1) compared to the NC diet. The super dose of phytase in the PC+ diet significantly improved the overall FCR (p < 0.1). For Experiment 2, the impact of different calcium (Ca)/phosphorous (P) ratios with or without supplementation of vitamin D3 was determined. Ninety-six piglets were randomized into four treatments with 6 replicates (4 piglets·cage-1). The PC, NC1 and NC2 diets had ratios of 0.80 : 0.50, 0.80 : 0.45, and 0.60 : 0.45%, respectively. The fourth diet (PCV) was the PC diet fortified with vitamin D3. Improved results were observed with the PC diet (p > 0.1) compared to the NC diets. Supplementing dietary 25-OH-D3 in the PCV diet led to numerically higher ADG and ADFI scores with lower FCR values at the early nursery stage (p = 0.0044), but the vitamin supplement did not significantly influence the growth.

Preparation and Quality Characteristics of Different Mackerel Scomber japonicas-based Processed Products as Senior-friendly Seafoods (물성을 달리한 고등어(Scomber japonicus) 활용 고령친화식품의 제조 및 품질특성)

  • Jang, Mi-Soon;Oh, Jae-Young;Kim, Poong-Ho;Park, Sun Young;Kim, Ye Youl;Kang, Sang In;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.5
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    • pp.703-713
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    • 2021
  • Mackerel Scomber japonicus is an excellent source of vitamins (A, D and B12), omega-3 fatty acids, dietary protein and minerals. This study was conducted to prepare mackerel-based processed seafood products such as grilled product with super-heated (GM), tang (TM) and salad with sweet potato (SSM) using mackerel as senior-friendly seafoods and to examine their quality characteristics. The hardness of GM, TM and SSM were 240.3×1.03, 21.7×1.03 and 7.4×1.03 N/m2, respectively. The viscosity of SSM was 25,450 m·Pas. The nutritional content of mackerel-processed products was within the senior-friendly standard specifications for protein, vitamin C, vitamin B2 (riboflavin) and vitamin B3 (niacin) ing GM, protein, vitamin B3 and calcium for TM, and protein, vitamin D, vitamin B2 and vitamin B3 in SSM. All the mackerel-processed products were safe as senior-friendly seafoods, since digestibility rates were 81.5% for GM, 87.9% for TM and 93.5% for SSM. The physical, nutritional and the sanitation results indicated that senior-friendly seafoods classified of the mackerel-processed products was step 1 for GM, step 2 for TM and step 3 for SSM according to the Korean Food Code and Korean Indusrty Standards.

Growth Characteristic of Pinus densiflora by Soil Generated at Civil Works Site (현장발생토 활용 식재기반 조성유형별 소나무 생육 특성 평가)

  • Oh, Deuk-Kyun;Kim, Phil-Lip;Yoon, Yong-Han;Kim, Won-Tae
    • Journal of Environmental Science International
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    • v.28 no.8
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    • pp.655-667
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    • 2019
  • This research aims to identify the possibility of developing A horizon resources that can be used for construction and civil engineering work. As such, the utility of A horizon resources was examined by establishing planting ground through a mixture of soil layers and by analyzing the growth and development of Pinus densiflora. The physicochemical and physical properties of the soil were as follows: the A horizon was sandy clay loam, B horizon was sandy loam, and the mixture of two layers appeared as sandy loam, which was identical to the B horizon. The experimental groups did not show any significant difference in their physical properties of porosity and degree of water-stable aggregates. With regards to chemical properties, the A horizon as well as the mixture of A and B horizon showed acidity while the B horizon showed alkalinity. The figures of organic matter, total nitrogen, available phosphate, and replaceable potassium were greater as the A horizon content increased, whereas the figures of replaceable calcium, replaceable magnesium, and conductivity increased as the A horizon content decreased. As a result of the growth and development of Pinus densiflora in each planting ground, the final survival rates were all above 100%. However, the tree height and the rate of growth for the diameter of root were higher in the order of A horizon > A horizon + B horizon > B horizon,indicating that the increased A horizon content is related to the growth and development of Pinus densiflora. The treatment of soil with improvement agents, used to recover the functions of in-situ soil showing poor growth and development, did not have a clear impact on the soil texture and porosity. However, the degree of water-stable aggregates increased significantly when using O horizon as the soil improvement agent among the types of in-situ soil. In contrast, all items related to the chemical properties showed significant differences following the treatment by soil improvement agents. The survival rate according to the treatment of soil improvement agents for the growth and development of Pinus densiflora was higher in the order of organic horizon = no treatment > compound fertilizer > organic fertilizer + compound fertilizer > organic fertilizer; this result was statistically significant with a marginal significance value of the log-rank test(p < 0.05).

Transcriptional regulation of chicken leukocyte cell-derived chemotaxin 2 in response to toll-like receptor 3 stimulation

  • Lee, Seokhyun;Lee, Ra Ham;Kim, Sung-Jo;Lee, Hak-Kyo;Na, Chong-Sam;Song, Ki-Duk
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1942-1949
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    • 2019
  • Objective: Leukocyte cell-derived chemotaxin 2 (LECT2) is associated with several physiological processes including inflammation, tumorigenesis, and natural killer T cell generation. Chicken LECT2 (chLECT2) gene was originally identified as one of the differentially expressed genes in chicken kidney tissue, where the chickens were fed with different calcium doses. In this study, the molecular characteristics and gene expression of chLECT2 were analyzed under the stimulation of toll-like receptor 3 (TLR3) ligand to understand the involvement of chLECT2 expression in chicken metabolic disorders. Methods: Amino acid sequence of LECT2 proteins from various species including fowl, fish, and mammal were retrieved from the Ensembl database and subjected to Insilco analyses. In addition, the time- and dose-dependent expression of chLECT2 was examined in DF-1 cells which were stimulated with polyinosinic:polycytidylic acid (poly [I:C]), a TLR3 ligand. Further, to explore the transcription factors required for the transcription of chLECT2, DF-1 cells were treated with poly (I:C) in the presence or absence of the nuclear factor ${\kappa}B$ ($NF{\kappa}B$) and activated protein 1 (AP-1) inhibitors. Results: The amino acid sequence prediction of chLECT2 protein revealed that along with duck LECT2 (duLECT2), it has unique signal peptide different from other vertebrate orthologs, and only chLECT2 and duLECT2 have an additional 157 and 161 amino acids on their carboxyl terminus, respectively. Phylogenetic analysis suggested that chLECT2 is evolved from a common ancestor along with the actinopterygii hence, more closely related than to the mammals. Our quantitative polymerase chain reaction results showed that, the expression of chLECT2 was up-regulated significantly in DF-1 cells under the stimulation of poly (I:C) (p<0.05). However, in the presence of $NF{\kappa}B$ or AP-1 inhibitors, the expression of chLECT2 is suppressed suggesting that both $NF{\kappa}B$ and AP-1 transcription factors are required for the induction of chLECT2 expression. Conclusion: The present results suggest that chLECT2 gene might be a target gene of TLR3 signaling. For the future, the expression pattern or molecular mechanism of chLECT2 under stimulation of other innate immune receptors shall be studied. The protein function of chLECT2 will be more clearly understood if further investigation about the mechanism of LECT2 in TLR pathways is conducted.

Analysis of Physiological Activity and Cytotoxicity of Residue Extracts after Pepper Harvest (고추 수확 후 잔재물 추출물의 생리활성과 세포독성 분석)

  • Kim, Tae-Won;Jeon, Byeong-Gyun;Lee, Sung-Ho
    • Journal of Life Science
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    • v.30 no.12
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    • pp.1085-1091
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    • 2020
  • The purpose of this study was to investigate the value of residue discarded after harvesting a species of pepper (Capsicum annuum L.). This study analyzed the physiological activity and cytotoxicity of a fermented extract and a hot water extract gathered from the residues of the Cheongyang pepper and the Nokkwang pepper. The total nitrogen, phosphoric acid, potassium, calcium, and magnesium contents of the fermented extract of the Nokkwang pepper were approximately twice as high as those of the Cheongyang pepper, while the hot water extracts had similar levels of these components across both peppers. Among the microelements, only boric acid, iron, and silicon components were detected, whereas zinc, manganese, molybdenum, and copper components were not detected in either extract. The total polyphenol and flavonoid contents of the fermented extracts were more than two times higher than those of the hot water extracts for both peppers. The DPPH radical scavenging ability (indicating antioxidant activity) of the fermented extract was higher than that of the hot water extract, while the ABTS radical scavenging ability (indicating antioxidant activity) of the hot water extract was higher than that of the fermented extract. A cytotoxicity test was conducted on the extracts using an MTT assay. Both extracts exhibited weak cytotoxicity at all concentrations for both peppers. These results suggest that the extract of residue discarded after harvesting these peppers could be suitable for functional feed or agricultural materials.

Expression Analysis of OsCPK11 by ND0001 oscpk11 Mutants of Oryza sativa L. under Salt, Cold and Drought Stress Conditions (염분, 저온 및 가뭄 스트레스 조건에서 벼 ND0001 oscpk11 돌연변이체의 OsCPK11 발현 분석)

  • Kim, Hyeon-Mi;Kim, Sung-Ha
    • Journal of Life Science
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    • v.31 no.2
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    • pp.115-125
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    • 2021
  • Calcium-dependent protein kinases (CDPKs) are known to be involved in regulating plant responses to abiotic stresses such as salinity, cold temperature and dehydration,. Although CDPKs constitute a large multigene family consisting of 31 genes in rice, only a few rice CDPKs' functions have been identified. Therefore, in order to elucidate the functions of OsCPK11 in rice, this study was intended to focus on the expression pattern analysis of OsCPK11 in wild type and ND0001 oscpk11 mutant plants under these abiotic stresses. For the salt, cold and drought stress treatment, seedlings were exposed to 200 mM NaCl, 4℃ and 20% PEG 6,000, respectively. RT-PCR and quantitative real-time PCR were performed to determine the expression patterns of OsCPK11 in wild type and ND0001 mutant plants. RT-PCR results showed that OsCPK11 transcripts in the wild type and heterozygous mutant were detected, but not in the homozygous mutant. Real-time PCR results showed that relative expression of OsCPK11 of wild type plants was increased and reached to the highest level at 24 hr, at 6 hr and at 24 hr under salt, cold and drought stress conditions, respectively. Relative expression of OsCPK11 of ND0001 homozygous plant was significantly reduced compared to that of wild type. These results suggested that oscpk11 homozygous mutant knocks out OsCPK11 and OsCPK11 might be involved in salt, cold and drought stress signaling by regulating its gene expression.