• 제목/요약/키워드: Cage protein

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Crystallization of amorphous Si by pulse annealing with Ni ferritins

  • Tojo, Yosuke;Miura, Atsushi;Fuyuki, Takashi;Yamashita, Ichiro;Uraoka, Yukiharu
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.553-556
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    • 2009
  • We investigated an application of supramolecular protein, and demonstrated the metal induced lateral crystallization utilizing ferritins with Ni nanoparticles, named the "bio-nano-crystallization". So far, this method has required long time, because of this method condition based on the conventional solid phase crystallization. In this study, we applied the pulsed rapid thermal annealing to bio-nanocrystallization. As a result, we succeeded in the crystallization for a short time. We found that the TFTs characteristics were improved with decrease metal impieties in poly-Si thin films by this method.

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Nutrition Practice to Alleviate the Adverse Effects of Stress on Laying Performance, Metabolic Profile, and Egg Quality in Peak Producing Hens: I. The Humate Supplementation

  • Hayirli, Armagan;Esenbuga, N.;Macit, M.;Lacin, E.;Karaoglu, M.;Karaca, H.;Yildiz, L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.9
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    • pp.1310-1319
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    • 2005
  • This experiment was conducted to determine the effects of cage density (CD) and humate supplementation (HS) on laying performance, metabolic profile, and egg quality during the peak production period in hens. Lohman layers (n = 180, 46 wks of age) were blocked according to the location of cages and then allocated randomly to two levels of CD (4 or 6 hens per cage or 540 vs. 360 $cm^2$/hen) and three levels of HS (0, 0.15, and 0.30%). Egg production (EP) and feed consumption (FC) were measured daily; egg weight was measured bi-weekly; and BW was measured before and after the experiment. Blood and additional egg samples were obtained at the end of the experiment for determination of metabolic profile and egg quality. The data were analyzed using two-way ANOVA as repeated measures. Except for FC, CD did not affect laying performance parameters. Hens placed in high-density cages had lower FC than hens placed in normal-density cages. Increasing HS level linearly increased FC, EP, and feed conversion ratio (FCR). There was a CD by HS interaction effect on FC and EP. Hens placed in high-density cages had greater serum glucose, total protein, albumin, globulin, Ca, and P concentrations and tended to have greater serum corticosterone concentration than hens placed in normaldensity cages. Increasing HS level linearly increased serum glucose, total protein, albumin, globulin, creatine, and Ca concentrations and linearly decreased serum triglyceride and very low-density lipoprotein concentrations. There was a CD by HS interaction effect on serum glucose and albumin concentrations. There were no alterations in egg quality parameters in response to increasing CD. Albumen index and Haugh unit decreased linearly and other egg quality parameters did not change as HS level increased. In conclusion, increased caging density adversely affected metabolic profile, despite insignificantly deteriorating laying performance. Moreover, benefits from humate supplementation seem to be more noteworthy for hens housed in stressing conditions than for hens housed in standard conditions.

Dual Effects of Calorie and Protein Restricted Diet Supplemented by Sugar on Albino Rat (주식에 첨가된 간식이 흰쥐의 체내대사에 미치는 영향)

  • Lee, Hyun-Kyong;Kim, Sook-He
    • Journal of Nutrition and Health
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    • v.6 no.4
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    • pp.23-36
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    • 1973
  • This study was designed to compare the effect of dietary intakes on different quality protein & levels of caloric consumption supplemented by sugar at the level of 26% of total caloric intakes. 30 males and same number of females of Albino rats, aged 30-40 days were devided into following six groups, 5 rats each. ACO Group: Ad libitum, Casein, no sugar group ACS Group: Ad libitum, Casein, 26% sugar supplemented group RBO Group: 50% restriction,Bean, no sugar group RBS Group: 50% restriction, Bean, 26% sugar supplemented group RAO Group: 50% restriction, Anchovy, no sugar group RAS Group: 50% restriction, Anchovy, 26% sugar supplemented group The rats were kept in individual cage and given 6 different diet for 12 weeks. The result of this study were elucidated as follow. Body weight gained and organ weight showed no significant differences between sugar supplemented group and the others. It was noteworthy that the shrinkage of female sex organ supplemented by sugar in the diet showed lower degree than that of compared groups in this study. In other word, degree of shrinkage due to protein-caloric restriction was decreased by sugar supplementation. Nitrogen Metabolism and total nitrogen retention were not observed any significant differences between sugar supplemented group and the others. Dental caries showed higher incidence for sugar supplemented groups. Hematology and bone growth showed no differences in this study. The similar results on the metabolic effects concerned the above view Points were obtained in the different protein groups such as bean & anchovy as protein sources in the diet. Caloric restriction Produced a lower growth-rate, lower body weight and poorer long bone growth. But composition of bone ash, concentration of nitrogen, calcium and blood glucose, liver fats and liver water content maintenanced at the same levels of standard group.

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In vivo and In vitro Digestibility of Enzyme-treated Fish Meal for Juvenile Atlantic Bluefin Tuna Thunnus thynnus (효소처리어분에 대한 치어기 대서양 참다랑어(Thunnus thynnus)의 in vivo 및 in vitro 소화율 평가)

  • Shin, Jaehyeong;Ji, Seung-Cheol;Lee, Kyeong-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.3
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    • pp.423-431
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    • 2020
  • This study was conducted to investigate in vivo and in vitro digestibility in juvenile Atlantic Bluefin tuna Thunnus thynnus. In vivo digestibility was compared between four experimental diets to determine the optimum dietary inclusion level of an enzyme-treated sardine fish meal (EFM) and sardine fish meal (FM). The experimental diets were as follows; EFM75 (75% EFM), EFM60 (60% EFM and 15% FM), FM75 (75% FM) and SL (frozen sand lance) as a raw fish feed. Feces of Bluefin tuna (90.3 g) were collected both by siphoning from a 700 L cage and by dissection in 69 ton concrete rearing tanks. For the siphoning method, protein digestibility was higher in the tuna fed SL diet than that of other groups. The lowest protein digestibility was observed in FM75. For the dissection method, protein digestibility was higher in tuna fed EFM75 diet than that of other groups. The lowest protein digestibility was observed in the EFM60 group. In vitro digestibility was compared in six protein sources to find an alternative source of EFM for the tuna feed. The highest in vitro digestibility was observed in EFM (92%) followed by low temperature FM (72%), meat meal (65%), feather meal (60%), sardine fish meal (57%) and poultry by-product meal (55%).

Effect of Protein and Protein Hydrolysate on Nitrogen Metabolism in Rats with Gastric Ulcer Induced by Restraint and Water-Immersion Stress (단백질과 단백질 가수분해물이 침수 속박 스트레스로 유도된 위 궤양 흰쥐의 질소대사에 미치는 영향)

  • 김창임
    • Journal of Nutrition and Health
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    • v.28 no.4
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    • pp.291-297
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    • 1995
  • This study aimed to verify the nutritional and curative effects of protein hydroysate in rats model with gastric ulcer induced by restraint and water-immersion stress. Sprague-Dawley, famale rats weighing approximtely 200g were forced in 5$\times$5$\times$15cm plexiglas cage. The restraint and water immersion stress was carried at 20$\pm$2$^{\circ}C$ for 8-hours. After stress 4 kinds of diets(10% casein, 20% casein, 10% casein hydrolysate, 20% casein hydrolysate) were given for 5 days. In the gastric ulcer rats model, the growth, gastric emptying rate, trypsin activity in gastrointestinal content, plasma total protein, albumin, $\alpha$-amino-N, UUN, creatinine and hydroxyproline of the urine and nitrogen retention were analyzed for nutritional effects of dietary nitrogen levels(10%, 20%) and sources (casein, casein hydrolysate). The results were as follows ; In gastric ulcer rats model, severeness of ulcer, plasma protein, gastric emptying rate, nitrogen retention rate were not different between 20% casein-fed group and 20% casein hydrolysatefed group. But 10% casein hydrolysate-fed group had more curative group. The casein hydrolysate diet-fed group was lower trysin activity in small intestianl content than the casein-fed group, at both casein level(10%, 20%). Finally at 20% levels, there was no difference between casein and casein hydrolysate diet, but 10% level, casein hydrolysate diet was more curative of ulcer than casein diet in gastric ulcer rat model. The results of this study provide useful information concerning diet therapy for the patients with gastrointestinal diseases and the field of enteral diet materials.

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Effect of Dietary Protein Levels and Addition of Alumincum Sulfate to Litter on Broiler Performance, Ammonia Production and Soluble Phosphorus Content of Litter (동일 축사내에서 Aluminum Sulfate 처리와 단백질 수준에 따른 육계의 생산성과 암모니아 가스 발생 및 깔짚내 수용성 P의 함량에 미치는 영향)

  • 최인학;남기홍
    • Korean Journal of Poultry Science
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    • v.30 no.2
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    • pp.113-118
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    • 2003
  • In this study, broilers were raised up to 6 weeks of age in a single room to determine if different levels of dietary protein or addition of aluminum sulfate[alum, $Al_2$(SO$_4$)$_3$ㆍ14$H_2O$] to the litter affected growth performance, production of ammonia(NH$_3$) and soluble phosphorus(SP) content of the litter.The experimental treatments consisted of six treatments in a 2x3 factorial arrangements: T$_1$=23% protein + 0.2% alum to litter; T$_2$=21% protein + 0.2% alum to litter; T$_3$=19% protein + 0.2% alum to litter; T$_4$=23% protein + no alum; T$_{5}$=21% protein + no alum; T$_{6}$=19% protein + no alum. For broiler performance, there was no effect of alum addition to the litter, but the dietary protein levels significantly affected feed intake from days 22 to 42(P<0.05) and day 0 to 42(P< 0.05), weight gain during all periods(P<0.05 or 0.01), and feed:gain from day 0 to 21(P<0.05) and day 0 to 42(P<0.05). Alum addition to the litter did not affect body weight at 21 and 42 days, but dietary protein levels has a significant effect on it at both 21(P<0.0l) and 42 days(P<0.05). Alum addition only affected ammonia production at weeks 3(P

Reduction of Mouse Body Fats by Water Extract of Pleurous Ostreatus

  • Kim, Seck -Jong;Park, Cherl -Woo;Kim, Jeong-Ok;Kim, Jong -Man;Ha, Yeong-Lae
    • Preventive Nutrition and Food Science
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    • v.4 no.2
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    • pp.130-133
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    • 1999
  • Body fat-reducing ability of oyster mushroom (Pleurotus ostreatus) water extract (OMWE) was investigated of mice by supplying it drinking water. OMWE(2.95% solid content ) was prepared by extracting a low grade of the mushroom at 12$0^{\circ}C$ for 10min. The solid material of OMWE was composed of 65.2% reducing sugar, 0.23% crude fat, 0.5%total protein, 1.2% ash and 32.9% others. OMWE was appropriately diluted with drinking water. Seventy two male ICR mice(25$\pm$1 g, 7~8 weeks of age, 6 mice/cage, 18 mice/treatment) housed in polycarbonate cages containing $\beta$-chips were adopted in a temperature-and humidity-controlled facility with free access to water and diet. One week later, the mice were subjected to one of the treatments for 36days : 0 (control), 10, 50 and 100% OMWE. Drinking wter with or without OMWE was supplied twice (40ml each, 80ml in total ) daily per cage. Body weight and fed intake were recorded every three days. At the end of the experiment, mice were sacrificed to determine the chemical composition (fat, protein, ash and water). Body weight of mice treated with OMWE (10, 50 and 100%) at day 36 was 35.9, 35.9and 35.5g per mouse , respectively, and not significantly reduced as compared to that (36.5g/mouse) of control mice. Average body fat of 0,10,50 and 10% OMWE -treated mice was 14.3, 13.1, 10.7 and 12.0% , respectively. Body fat reduction by 50% OMWE treatment was 25.2% (p<0.05) relative to control. OMWE did not affect feed intake. The contents of body protein and ash were increased with respect to body fat decrease, while water content was not changed much. These results suggest that OMWE could reduce body fat of the mice without body weight change, giving the best effect by 50% OMWE.

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A review of the mass-mortalities of sea-cage farm fishes (해상 가두리양식장 양식어류의 대량폐사에 대하여)

  • Han, Jido;Lee, Deok-Chan
    • Journal of fish pathology
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    • v.35 no.1
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    • pp.1-25
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    • 2022
  • The aquaculture industry has developed rapidly over the last three decades and is an important industry that supplies over 15% of humans' animal protein intake; therefore, there is a need to increase production to meet the continuous demand. The fish cage farms on the southern coast (Kyengsangnam-do and Jeollanam-do) of Korea are critical resources in aquaculture because they account for approximately 90% of the national total fish cage farms by water area ratio. However, the current aquaculture environment is being gradually affected by climate change, which is a global issue, and its effects are expected to intensify in the future. Therefore, it is urgently imperative to accurately evaluate the effects of climate change on South Korean aquaculture industries and to develop social and national strategies to minimize damage to the fishing industry. The damage to fish farmed in cage farms on the southern coast is increasing annually and the leading causes are high and low water temperature and red tides, which are directly or indirectly related to climate change. At present, global warming can provide opportunities for aquaculture industrialization of fish or other novel species, with economic implications. However, despite such opportunities, the influx of new species can also cause problems such as ecological disturbances, increase in the reproduction frequency of microalgae such as red tide, increase in disease incidence, and occurrence and periods of high water temperatures in summer. The scale of farmed fish mortality is increasing due to the complex effects of these factors. Increased damages due to fish mortality not only have severe economic impacts on the aquaculture industry, but the social costs of responding to the damage and follow-up measures also increase. various active responses can reduce the mortality damage in fish farms such as improving the management skills in aquaculture, improved species breeding, efficient food management, disease prevention, proactive responses, and system-wide improvements. This review article analyzes the large-scale mortality cases occurring in fish cage farms on the southern coast of Korea and proposes measures to mitigate mortality and enhance responses to such scenarios.

SESAME MEAL AS SOYBEAN MEAL SUBSTITUTE IN POULTRY DIETS II. LAYING HEN

  • Cheva-Isarakul, B.;Tangtaweewipat, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.253-258
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    • 1993
  • The study on the nutritive value and the potential use of two kinds (local vs. import) of sesame meal (SSM) in layer diets as substitute for soybean meal (SBM) at 25, 50, and 75% was carried out. Three hundred and thirty six layers were kept individually on a battery cage and fed isonitrogenous diets, 16% crude protein (CP), for 252 days. The local and the imported SSM contained on air dry basis 35.7 and 36.3% CP, 24.7 and 9.1% ether extract (EE), respectively. Their amino acid contents are all lower than those reported by NRC (1984). The local material contained remarkably lower lysine (0.44 vs. 0.94), methionine + cystine (1.06 vs. 1.75) and threonine (0.52 vs. 1.22) than the imported meal. Imported SSM could be substituted for SBM as high as 50% in layer ration without statistically adverse effect on egg production, feed intake, body weight gain, average egg weight and egg size, while local SSM was inferior to the control in all aspects. However, when production performance of the groups fed either kind of SSM was compared, no significant difference was found at the two lower substitution levels.

Responses in growth performance and nutrient digestibility to a multi-protease supplementation in amino acid-deficient broiler diets

  • Cho, Hyun Min;Hong, Jun Sun;Kim, Yu Bin;Nawarathne, Shan Randima;Choi, Inchul;Yi, Young-Joo;Wu, Di;Lee, Hans;Han, Seung Eun;Nam, Ki Taeg;Seoung, Eun Il;Heo, Jung Min
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.840-853
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    • 2020
  • The present study was conducted to investigate the effect of a multi-protease on production indicators of broiler chickens fed a crude protein and amino acid deficient-diets for 35 days immediately after hatch. A total of 448 one-day-old Ross 308 male broiler chicks were allocated in a completely randomized design into one of eight dietary treatments (positive control [PC], negative control [NC: minus 0.5% from PC, and minus 2% of lysine, methionine, threonine and methionine plus cysteine], extreme negative control [ENC: minus 1% from PC, minus 4% of lysine, methionine, threonine and methionine plus cysteine], and plus multi-protease 150 or 300 g per ton [e. g., PC-150]; PC, PC-150, NC, NC-150, NC-300, ENC, ENC-150, ENC-300) to give eight replicates with seven birds in a battery cage. Body weight, average daily gain, average daily feed intake, feed conversion ratio, and mortality were measured every week. Carcass traits, proximate analysis of breast meat, and ileum digestibility were analyzed on day 21 and 35. Feeding a multi-protease (i.e., more than 150 g/ton) for 35 days immediately after hatching improved feed efficiency and ileum digestibility (i.e., dry matter, crude protein, and energy) compared to their counterparts (i.e., diets without multi-protease: PC, NC, and ENC). In conclusion, our results indicated that broiler chickens fed nutrients deficient-diet (i.e., crude protein and amino acids) supplemented a multi-protease had an ability to compensate and (or) improve their growth performance commensurate with increased ileal digestibility for 35 days immediately after hatch.