• Asian-Australasian Journal of Animal Sciences
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• 제18권12호
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• pp.1752-1760
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• 2005

In this experiment, the effects of cage density (CD) and probiotic supplementation (PS) on laying performance, metabolic profile, and egg quality in peak-producing hens were evaluated. After blocking according to the cage location, Lohman layers (n = 180, 46 wks of age) were allocated randomly to two levels of CD (540 vs. 360 $cm^2$/hen) and three levels of PS (0, 0.15, and 0.30%). Probiotic contained Enterococcus faecium culture (10${\times}$10$^9$ cfu/g). Egg production (EP) and feed consumption (FC) were measured daily; egg weight (EW) was measured bi-weekly; BW was measured before and after the experiment; and blood samples were obtained at the end of the experiment. The data were analyzed using two-way ANOVA. Increasing CD decreased FC (125.0 vs. 120.8 g/d, p<0.0001) and FCR (1.93 vs. 1.87, p<0.0001) and did not alter EP, EW, and BW. Increasing level of PS linearly decreased FC (p<0.02) and FCR (p<0.006). Averages were 123.9, 123.2, and 121.6 g/d for FC and 1.91, 1.92, and 1.86 for FCR in hens supplemented with 0, 0.15, and 0.30% probiotic, respectively. Hens placed in high-density cages had greater serum corticosterone concentration than hens placed in normal-density cages (12.8 vs. 11.3 $\mu$g/dL, p<0.04); CD did not affect concentrations of other metabolites. Increasing level of PS linearly increased serum glucose, albumin, and creatine concentrations and quadratically increased total protein, globulin, Ca, and P concentrations. Average concentrations (mg/dL) were 260, 297, and 305 for glucose; 6.28, 8.09, and 7.58 for total protein; 1.98, 2.48, and 2.38 for albumin; 4.30, 5.62, and 5.19 for globulin; 0.40, 0.52, and 0.54 for creatine; 16.0, 16.5, and 16.3 for Ca; and 6.27, 8.14, and 7.17 for P in hens supplemented with 0, 0.15, and 0.30% probiotic, respectively. There was no effect of CD on egg quality. Increasing level of PS linearly improved yolk color (YC) and quadratically increased albumen index (AI) and Haugh unit (HU). The mean values were 9.67, 9.75, and 10.58 for YC; 8.94, 6.93, and 8.72% for AI; and 85.6, 74.9, and 82.9 for HU for hens supplemented with 0, 0.15, and 0.30% probiotic, respectively. There was also CD by PS effect on FC, EP, and serum glucose, total protein, albumin, globulin, creatine, Ca and P concentrations. In conclusion, increased CD partially depressed laying performance and caused stress. Probiotic supplementation improved laying performance and metabolic profile. It also partially alleviated the adverse effects of stress resulting from increased caging density.

• 동아시아식생활학회지
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• 제7권1호
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• pp.13-20
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• 1997

This study was designed to determine the effects of protein sources on the curation of gastric ulceration, protein metabolism, and nitrogen balance in rats with gastric ulcer induce by restraint and water immersion stress. After the rats were fed 10% casein diet for 3 weeks, four groups of the rats were forced in 5$\times$5$\times$15cm plexiglass cage. The restraint and water immersion stress was carried at 20$\pm$2$^{\circ}C$ for 8-hour. The other one group(control group) was not exposed to stress. After stress 4 kinds of different diets containing 20% protein were given for 5 days. The protein sources were casein, whey protein, soy protein, gluten. The control group was fed to 10% casein diet. The results were as follows ; the weights of rats were not different among the diet groups During the experiment period follows ; the weights of rats were not different among the diet groups during the experiment period (for 5 days). The ulcer index of rats fed 10% gluten and soy protein diet was significantly higher than those of casein and whey protein diet groups(p<0.05). The level of serum albumin was not significantly different among diet groups. But hematocrit and the level of $\alpha$-amino-N, BU and UUN of plant protein diet groups were higher than animal diet groups, the urinary hydroxyproline of soy protein group was the highest and the whey protein was the lowest. The digestibility and BV of nitrogen of gluten diet group were significantly higher than those of casein and whey protein diet groups(p<0.05). The animal proteins had more curative effects of ulcer than plant animals. The results of this study provide useful information concerning diet therapy for the patients with gastrointestinal diseases and the field of enteral diet materials.

• 수산경영론집
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• 제29권1호
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• pp.121-137
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• 1998

This study was conducted to examine the economic evaluation of the newly developed fish meal analog (BAIFA-M) in Korean rockfish feed. A raw fish feed (MP) and two commercially formulated diets (EP) were employed to compare weight gain (WG), feed conversion ratio (FCR) and survival rate (SR) from the sea cage culture system. By using the economic model in the practical sea cage culture system, feed cost, production cost and gross profit per kg production, rate of profit to gross revenue (RPGR), and internal rate of return (IRR) were calculated based upon the results of the experiment and the information of the private aquaculture farm (Young Chang) in Tong young, Korean. IRR criteria is one of the popular economic feasibility analysis methods applicable far aquaculture industry. This is an economic evaluation method to compare the given interest rate or the discount rate with IRR which can be calculated by the difference between the present value of the benefit stream and of the cost stream. The benefits of using EP on WG, FCR, SR, and production cost will be emphasized in this study. Fish averaging 20$\pm$3.6g (Mean$\pm$SD) were randomly distributed in each small cage (6m$\times$6m) as groups of 2,000 fish. By using 3 large size cages (12m$\times$12m), 12 small cages were constructed, and only 9 small cages were employed for three replicates of each diet treatment. To compare with MP diet, two sinking EP diets were designed by our laboratory and produced by the local feed company who wanted to promote these EP diets for the mass cage culture of Korean rockfish in the future. Two EP diets contain white fish meal and/or BAIFA-M as the main animal protein sources : WFM diet, maximum 43% of white fish meal : BAIEA- M diet, 30% of white fish meal nab replaced by BAIFA- M from WFM diet. Results are summarized in Table 1. Fish fed MP diet showed significantly lower SR than does fish fed two EP diets(P<0.05). However, there Were no significant difference on FCR among fish fed three practical diets. Table 1. Average feed conversion ratio (FCR), accumulative average survival rate (SR) and economic evaluation data far three practical diets. As we expected, BAIFA-M diet is more economical than WFM diet as well as MP diet. Feed cost and production cost per kg production from BAIEA - M diet were lower than those from WFM and MP diets. Moreover, gross profit per Kg production, RPGR and IRR from BAIFA- M diet were higher than those from WFM and MP diets. This economic evaluation study clearly indicated that MP diet should be replaced by the commerical formulated EP diets as soon as possible in the near future because MP diet is not economical in the practical sea cage culture system.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2005년도 추계종합학술대회
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• pp.755-758
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• 2005

This paper describes the novel immunoassay sensing system for a portable clinical diagnosis system. It consists of a bead cage reactor and a CMOS integrated biosensor. It showed the simple and easy antibody coating method on beads by flow-through avidin biotin complex technology in a microfluidic device. It showed just 90 nL sample consumption and good result for the application of alpha feto protein. The bead cage reactor has the role of the antibody coating, antigen binding and enzyme linking for the electrochemical sensing method. The CMOS biosensor consists of ISFET (ion selective field effect transistor) biosensor and temperature sensor for detecting pH that is the byproduct of enzyme reaction. The sensitivity is 8 $kHz/^{\circ}C$ in a temperature sensor and 33 mV/pH in a pH sensor. After filling the 15 um polystyrene beads in bead cage, antibody flowed and reacted to beads. Subsequently, the biotinylated antigen flowed and bound to the antibody and GOD (glucose oxidase)-avidin conjugate flowed and reacted to the biotin of the biotinylated antigen. After this reaction process, glucose solution flowed and reacted to the GOD on beads. The hydrogen was generated by glucose-GOD reaction. And it was detected by the pH sensor.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.45-51
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• 1993

With the purpose to utilize cage layer manure (CLM), whole crop corn forage was ensiled with 30% CLM (MS silage) and without CLM (CS silage). MS silage was significantly (p < 0.05) higher in pH value, total VFA, propionic and butyric acids, and the ratio of ammonia nitrogen to total nitrogen, but lower (p < 0.05) in lactic acid and water soluble carbohydrate (WSC) contents than CS silage. Digestibility was evaluated using Shiba strain Japanese goats. Urea was supplemented to CS silage at feeding to adjust nitrogen intake to MS silage (US silage). There were no differences in digestibilities of dry matter (DM) and energy among the three silages. However, US silage showed higher (p < 0.05) digestibility of crude protein, but digestibilities of NDF, ADF, hemicellulose and cellulose were higher (p < 0.05) in MS silage. Nitrogen retention was positive in US and MS silages, but it was negative in CS silage. Voluntary feed intake of goats was 11.02, 12.03 and 13.34 g of DM per metabolic body weight ($kg^{0.75}$) for 10 minutes, for CS, US and MS silages, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.53-59
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• 1993

In situ degradability and fermentation characteristics in the rumen of goats fed whole crop corn forage ensiled with (MS silage) or without (CS silage) 30% of cage layer manure (CLM) were investigated. The two silages were well preserved. To adjust nitrogen intake of CS silage to that of MS silage, the 3rd group of goats was given urea with CS silage at feeding time (US silage). Each goat was given a diet of 2% of the body weight (dry matter basis) daily. In situ degradability of dry matter (DM) and crude protein (CP) of MS silage in the rumen were higher than those of CS and US silages. Total potentially degradable portions of DM and CP in MS silage were also higher than those in CS and US silages. Blood urea nitrogen and rumen ammonia nitrogen concentration of goats fed US and MS silages were significantly (p<0.05) higher than those of goats fed CS silage. Acetic, propionic and butyric acids in ruminal fluids of goats fed MS silage were significantly (p<0.05) higher than those of goats fed CS and US silages.

• 한국가금학회지
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• 제44권2호
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• pp.67-73
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• 2017

The purpose of our study was to determine the effects of varying levels of energy, protein, and amino acids on the performances of laying hens. A total of 240 Hy-Line Brown laying hens at 36 weeks of age were used in this 4-week feeding trial. The hens were randomly allocated to five treatment diets, with eight replications of six hens in each replicate cage. The treatment diets were as follows: A- basal diet + 18% crude protein, metabolizable energy 2,800 kcal, total (methionine + cysteine) 0.65%; B- basal diet + 17% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; C- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; D- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.54%; and E- basal diet + 16% crude protein, metabolizable energy 2,680 kcal, total (methionine + cysteine) 0.54%. The study results revealed that the hen-day egg production of hens that were fed with low-energy diets (B, C, and D) was comparable with that of hens fed with high-energy diet A, whereas average daily feed intake in hens fed treatment diet D and E was significantly higher (P<0.05) than that in hens fed treatment diet A. Overall, the eggshell thickness was unaffected by any of the treatment diets. Egg weight was comparable among the treatment diets, except for treatment diet E. Haugh unit improved with decreasing levels of dietary energy, protein, and methionine + cysteine in the diet. We can summarize that laying hens fed with low dietary energy and low crude protein treatment diets B, C, and D had satisfactory performance compared with those fed with high-energy treatment diet A. This indicates that there is the potential to reduce feed costs by formulating diets with lower energy and low protein levels.

• 한국양식학회지
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• 제13권3호
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• pp.259-265
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• 2000

Four experimental diets were formulated to contain white fish meal (WFM) andvor BAIFA-M as the main animal protein source to determine the optimum BAIFA-M level to replace WFM protein. The diets contained 100% WFM + 0% BAIFA-M in the diet M$_0$ (control) . 70%, WFM+30%, BAIFA-M in the diet M$_{30}$, 55 % WFM+45 % BAIFA-M in the diet M$_{45}$, ; 40 % WFM+60 % BAIFA-M in the diet M$_{60}$. The four groups of rockfish (232 g), receiving the four different diets, were fed for 8 weeks. Groups receiving M$_0$, M$_{30}$ and M$_{45}$/ diets did not show significant (P>0.05) difference in growth and feed efficiency; however, the group fed on M$_{60}$/ showed significantly slower growth and lower feed efficiency than the others. Body composition showed no significant difference among the four dietary groups. WFM protein can be replaced by BAIFA-M up to 45 % in the diet of the rockfish.

• 한국축산시설환경학회지
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• 제5권1호
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• pp.63-72
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• 1999

This study was carried out to improve utilization of fly ash. Each animal waste was mixed with fly ash and composted This compost used at forage crops with corn, rye and alfalfa to examine to examine the fertilized efficiency and investigated productivity of forage crops, composition of this copmost and effect of fly ash on soil characteristics and composition. Content of organic matte, P2O5, K2O, CaO, MgO, Mn and B at the soil, which is given fly ash, increased. After the test crops were harvested, pH of the soil was maintained about 7 and contents of organic matter, phosphoric aicd, K, Mg, and B was increased at the soil of used fly ash. As fly ash was mixed, each DM yield of corn and rye was increased 10∼13% and 14∼21% especially alfalfa was increased 35% at the soil which is mixed fly ash with cage layer manure. As fly ash was mixed, each Crude protein (CP) of corn and rye was increased 6∼17% and about 29%, especially, as fly and cage layer manure was mixed CP of alfalfa was increased 33%. In conclusion, as fly ash is mixed with anlmal waste and use at forage crops, It makes the soil good and improve the productivity of forage crops.

• 한국해양생명과학회지
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• 제7권2호
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• pp.163-170
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• 2022

본 연구에서는 해상가두리 양식장에서 북방전복과 둥근전복속 교잡종(왕전복♀^*둥근전복♂)의 생물지표를 분석하여 교잡육종의 결과를 평가하고자 하였다. 생존율은 북방전복과 유사하였으나, 성장(각장)은 교잡종에서 약 10% 빠른 것으로 분석되었다. 패각의 호흡공 기형율은 교잡종이 북방전복보다 약 6% 낮았으며, 패각 함몰 및 부식율은 교잡종이 약 15% 낮았다. 생화학적 조성에서는 조단백질의 경우 교잡종에서 약 3.1% 높았으며, 이를 제외한 나머지에서 유사한 값을 나타냈다. 소화, 흡수 및 해독기능을 수행하는 간췌장의 조직학적 평가에서는 교잡종에서 좋은 결과를 보였다. 이러한 결과로 보아 둥근전복속 교잡종은 추후 양식 환경에 접목하였을 때 높은 양식 생산력을 가질 것으로 판단된다.