• Animal Bioscience
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• 제35권3호
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• pp.444-452
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• 2022

Objective: An experiment was conducted to evaluate the effect of different levels of crude protein (CP) and two rearing systems (cage and floor), on blood parameters and digestive and reproductive organ development of brown laying hens. Methods: A total of 400 Hisex Brown laying hens between 30 and 45 weeks of age were distributed in a completely randomized design and a 2×4 factorial arrangement, with main effects including two rearing systems (cage and floor) and levels of CP (140, 150, 160, and 180 g/kg), in a total of eight treatments and five replicates of 10 birds each with initial body weight of 1,877 g (laying hen in cage) and 1,866 g (laying hens in floor). The parameters evaluated were plasma total protein, albumin, uric acid, total cholesterol, relative weights of oviduct, abdominal fat, liver, gizzard, crest and dewlap, length of small intestine and oviduct. Results: The blood parameters were similar in birds reared in cage and floor systems. The birds reared on the floor showed greater small intestine and oviduct weight (%) and lower liver and pancreas weight (%). A significant interaction was observed between factors for the relative gizzard, crest and dewlap weight, serum protein, uric acid, and total cholesterol (p<0.05). The diets with 140 g/kg CP resulted in lower serum protein and lower cholesterol in birds reared in floor system, while birds reared in cage system showed no effect of CP on both parameters. Birds reared in cage and fed with 140 and 150 g/kg CP presented lower uric acid. The group of birds reared in floor system fed 180 g/kg had greater uric acid. Conclusion: The dietary protein level can be reduced up to 140 g/kg for Hisex Brown hens (30 to 45 weeks of age) without an important effect on metabolic profile and organ development in both rearing systems.

• Asian-Australasian Journal of Animal Sciences
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• 제13권11호
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• pp.1548-1553
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• 2000

An experiment was conducted to study the nutritional quality of whole crop corn silage ensiled with cage layer manure in sheep. Treatments were designed as a $3{\times}3$ Latin square with 16-day periods. Sheep were allotted in one of three diet-treatments, which were whole crop corn silage (CS), whole crop corn+30% cage layer manure (CLM) silage (based on DM; MS) and rice straw+concentrate (SC) mixed at 8:2 ratio (on DM basis). Silage ensiled with CLM significantly increased (p<0.05) digestibilities of crude protein, NDF and ADF, TDN over the other treatments. Ruminal pH in sheep fed SC was significantly (p<0.05) higher than that of the other diets at 0.5, 1, 2, 4 and 8 h after feeding. Ruminal ammonia nitrogen concentration of the MS treatment was significantly (p<0.05) higher than that of the other treatments at 0, 1, 2 h after feeding. The MS treatment highly increased (p<0.05) feed intake, digestibility of organic matter and crude protein, nitrogen intake and retained nitrogen. The MS treatment highly increased (p<0.05) purine derivative (PD) excretion leading to higher microbial protein synthesis.

• BMB Reports
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• 제55권4호
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• pp.175-180
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• 2022

Peptides are gaining substantial attention as therapeutics for human diseases. However, they have limitations such as low bioavailability and poor pharmacokinetics. Periostin, a matricellular protein, can stimulate the repair of ischemic tissues by promoting angiogenesis. We have previously reported that a novel angiogenic peptide (amino acids 142-151) is responsible for the pro-angiogenic activity of periostin. To improve the in vivo delivery efficiency of periostin peptide (PP), we used proteins self-assembled into a hollow cage-like structure as a drug delivery nanoplatform in the present study. The periostin peptide was genetically inserted into lumazine synthase (isolated from Aquifex aeolicus) consisting of 60 identical subunits with an icosahedral capsid architecture. The periostin peptide-bearing lumazine synthase protein cage nanoparticle with 60 periostin peptides multivalently displayed was expressed in Escherichia coli and purified to homogeneity. Next, we examined angiogenic activities of this periostin peptide-bearing lumazine synthase protein cage nanoparticle. AaLS-periostin peptide (AaLS-PP), but not AaLS, promoted migration, proliferation, and tube formation of human endothelial colony-forming cells in vitro. Intramuscular injection of PP and AaLS-PP increased blood perfusion and attenuated severe limb loss in the ischemic hindlimb. However, AaLS did not increase blood perfusion or alleviate tissue necrosis. Moreover, in vivo administration of AaLS-PP, but not AaLS, stimulated angiogenesis in the ischemic hindlimb. These results suggest that AaLS is a highly useful nanoplatform for delivering pro-angiogenic peptides such as PP.

• BMB Reports
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• 제42권11호
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• pp.758-763
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• 2009

Cancer-associated antigen (CAGE) induces the expression of matrix metalloproteinase-2 (MMP-2) by activating Akt, which in turn interacts with inhibitory kappa kinase $\beta$ ($I{\kappa}K{\beta}$) to activate nuclear factor ${\kappa}B$ (NF-${\kappa}B$). Akt and p38 mitogen activated protein kinase (p38 MAPK) are necessary for CAGE-mediated induction of the AP-1 subunit JunB, whereas extracellular regulated kinase (ERK) is necessary for the induction of fos-related antigen-1 (Fra-1). Induction of MMP-2 by CAGE requires activator of protein-1 (AP-1) to be bound. Specific binding of JunB to MMP-2 promoter sequences was shown by chromatin immunoprecipitation (ChIP) analysis.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.600-610
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• 2008

A cancer-associated antigen gene (CAGE) was identified by serological analysis of a recombinant cDNA expression library (SEREX). The gene was identified by screening cDNA expression libraries of human testis and gastric cancer cell lines with sera from patients with gastric cancer. CAGE was found to contain a D-E-A-D box domain and encodes a putative protein of 630 amino acids with possible helicase activity. The CAGE gene is widely expressed in various cancer tissues and cancer cell lines. Demethylation plays a role in the activation of CAGE in certain cancer cell lines where the gene is not expressed. The functional roles of CAGE in tumorigenesis, the molecular mechanisms of CAGE expression, and cell motility are also discussed.

• 한국초지조사료학회지
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• 제10권3호
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• pp.164-171
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• 1990

This study was carried out to enhance the nutritional value of whole crop corn silage and the utilization of animal waste as a ruminant feedstuffs. Whole crop corn were ensiled with cage layer excreta and concentrate. Treatments included whole crop corn silage (control), 10% cage layer excreta and concentrate-corn silage (CES lo), 20% cage layer excreta and 10% concentrate-corn silage (CES 20) and 30% cage layer excreta and 10% concentrate-corn silage (CES 30). The characteristics of silage fermentation was evaluated. Digestibility and palatability of the silage were observed through the feeding trial with four male Corridale sheep. The results obtained are summarized as follows: 1. Cage layer excreta-corn silage showed higher contents of crude protein and crude ash than control silage, while crude fibre content was significantlv lower than that of control silage (P(.01). 2. Total nitrogen and ammonia nitrogen content of the cage layer excreta-corn silage was significantly (P< .01) higher than that of control, but CES 10 silage was good in quality. 3. Lactic acid content of CES 10 silage was the highest, but that of CES 20 and CES 30 silage was lower than that of control. CES 20 and CES 30 silage was lower lactic acid content and higher pH than control. Therefore Flieg's score was wrose than that of control silage. 4. The numbers of total bacteria and lactic fermentation bacteria were estimated lo7-10' and 10"1OH, respectively, and there were no significant differences among treatments. Coliform numbers were not detected with CES 10 silage while were detected lo3 per gram in CES 30 silage. 5. Digestibility of crude protein, crude fibre and ADF was improved significantly in the animals fed the cage layer excreta-corn silage compared with animals fed the control silage. DCP and TDN were the highest in the CES 10 silage. 6. The intake of daily nitrogen and dry matter per metabolic body weight was the highest in animals fed the CES 10 silage.lage.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.84-84
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• 2013

FcBP consisting of 13 amino acids specifically binds to Immunoglobulin G Fc domain. Initially, we utilized this peptide for preparation of antibody chip as a PEG composite for enhanced solubility. After then, the peptide conjugate was immobilized on agarose resin, resulting in highly efficient affinity column for antibody purification. The efficiency was comparable to commercial Protein A column. Recently, this peptide was conjugated with cell penetratingpeptide (CPP) on a backbone of GFP, affording antibody transducer, which carries antibody into live cells by simple mixing of antibody and the transducer in cell culture media. Antibody transduction into cells was monitored by live cell imaging. More recently, the FcBP was fused to ferritin cage, which consists of 24 ferritin protein molecules. The FcBP-ferritin cage showed greatly increased binding affinity to human IgG. Its binding was analyzed by QCM and SPR analysis. Finally, it was selectively delivered by Herceptin to SKBR3, a breast cancer cell, over MCF10A, non-tumorigenic cells.

• Journal of Korean Neurosurgical Society
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• 제40권6호
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• pp.412-418
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• 2006

Objective : The safety of titanium metal cages in tuberculous spondylitis has not been investigated. We evaluated the outcome and complications of titanium mesh cages for reconstruction after thoracolumbar vertebrectomy in the tuberculous spondylitis. Methods : There were 17 patients with 18 operations on the tuberculous spondylitis in this study. Sixteen patients were operated with anterior corpectomy and reconstruction with titanium mesh cage followed by posterior transpedicular screw fixations on same day, two pateints were operated by either anterior or posterior approach only. After the affected vertebral body resection and pus drainage from the psoas muscle, titanium mesh cage, filled with morselized autogenous bone, was inserted. All the patients had antituberculosis medication for 18 months. The degree of kyphosis correction and the subsidence of cage were measured in the 15 patients available at a minimum of 2 years. Outcome was assessed with various cross-sectional outcome measures. Recurrent infection was identified by serial ESR[Erythrocyte Sedimentation Rate] and CRP[Cross Reactive Protein]. Results : There was no complication from the use of a titanium mesh cage. Recurrent infection was not detected in any case. Average preoperative of $9.2^{\circ}$ was reduced to $-2^{\circ}$ at immediate postoperative period, and on final follow up period kyphotic angle was measured to be $4.5^{\circ}$. Postoperatively, subsidence was detected in most patients especially at ambulation period, however further subsidence was prevented by the titanium mesh cage. Osseous union was identified in all cases at the final follow-up. Conclusion : The cylindrical mesh cage is a successful instrument in restoring and maintaining sagittal plane alignment without infection recurrence after vertebrectomy for tuberculous spondylitis.

• 한국가금학회지
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• 제14권1호
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• pp.1-8
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• 1987

사료의 에너지와 단백질 수준이 산란계의 능력에 미치는 영향을 조사하기 위하여 3회의 시험을 실시하였다. 시험1에서는 352일령의 마니나 브라운종을, 시험 2에서는 280일령의 갈색 와렌종을, 시험 3에서는 317일령의 갈색 와렌종을 각각 이용하였다. 각 시험에서 공히 총 360수의 공시계를 18수씩 20개구로 나누어 4개처리에 5반복으로 배치하였다. 시험 설계는 2가지 수준의 에너지(2500과 2800 kcal ME/kg)와 2가지 수준의 단백질 (13% 및 16 %)을 조합한 2$\times$2 요인시험을 실시하였다. 모든 공시계는 산란계용 Cage에 cage 당 2수씩 수용하였으며 시험 1과 2에서는 4주간의 시험기간동안 시험사료와 물을 자유로이 급여하였고 시험3에서는 2주간의 시험기간동안 사료섭취량을 1일 수당 130g으로 제한하여 급여하였다. 시험 1과 2에서 고에너지 사료를 섭취한 닭이 산란율이 다소 높았으나 유의성은 없었다. 그러나 시험 1에서는 단백질 수준이 산란율에 영향을 미치지 못하였다. 사료를 제한 급여한 시험3에서는 고에너지 사료에서 산란율이 개선되었으며(P〈0.05) 단백질 수준도 산란율에 영향을 미쳤으나 유의성은 없었다. 시험 1과 2에서 란중은 에너지 수준이나 단백질 수준에 의하여 영향을 받지 않았으나 시험 3에서는 고에너지 수준에서 란중도 약간 개선되는 경향을 보였다. 3회의 시험 모두에서 사료의 단백질이나 에너지 수준이 증가할 때 egg mass가 증가하는 경향을 보였다. 사료의 에너지나 단백질 수준이 증가할 때 사료섭취량은 감소하고 사료효율이 개선되는 경향을 보였다. 또한 사료의 에너지 수준이 증가할 때 단백질 효율(egg mass/단백질섭취량)이 개선되었으며 사료의 단백질 수준이 증가할 때 에너지 효율(egg mass /ME 섭취량) 이 개선되었다.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2559-2562
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• 2014