• The Korean Journal of Physiology and Pharmacology
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• 제12권6호
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• pp.315-321
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• 2008

Eugenol is widely used in dentistry to relieve pain. We have recently demonstrated voltage-gated $Na^+$ and $Ca^{2+}$ channels as molecular targets for its analgesic effects, and hypothesized that eugenol acts on $P2X_3$, another pain receptor expressed in trigeminal ganglion (TG), and tested the effects of eugenol by whole-cell patch clamp and $Ca^{2+}$ imaging techniques. In the present study, we investigated whether eugenol would modulate 5'-triphosphate (ATP)-induced currents in rat TG neurons and $P2X_3$-expressing human embryonic kidney (HEK) 293 cells. ATP-induced currents in TG neurons exhibited electrophysiological properties similar to those in HEK293 cells, and both ATP- and $\alpha$, $\beta$-meATP-induced currents in TG neurons were effectively blocked by TNP-ATP, suggesting that $P2X_3$ mediates the majority of ATP-induced currents in TG neurons. Eugenol inhibited ATP-induced currents in both capsaicin-sensitive and capsaicin-insensitive TG neurons with similar extent, and most ATP-responsive neurons were IB4-positive. Eugenol inhibited not only $Ca^{2+}$ transients evoked by $\alpha$, $\beta$-meATP, the selective $P2X_3$ agonist, in capsaicin-insensitive TG neurons, but also ATP-induced currents in $P2X_3$-expressing HEK293 cells without co-expression of transient receptor potential vanilloid 1 (TRPV1). We suggest, therefore, that eugenol inhibits $P2X_3$ currents in a TRPV1-independent manner, which contributes to its analgesic effect.

• 대한화장품학회지
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• 제32권1호
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• pp.7-15
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• 2006

본 연구에서는 발아 검은 쌀로부터 분리한 올리고펩타이드($Oligosproutin^{(R)}$)의 피부 탄력 개선 효과에 대하여 연구하였다. 올리고펩타이드가 각질형성세포의 유전자 발현에 미치는 영향을 알아보고자 DNA microarray를 수행하였다. 각질형성세포에 올리고펩타이드를 처리하여 유전자의 발현이 2배 이상 증가하는 유전자는 745개이고, 2배 이상 감소하는 유전자는 1011개로 나타났다. 이 중 hyaluronan synthase 2 (HAS2)의 유전자 발현이 2배 이상 증가함을 확인하였다. 올리고펩타이드를 처리한 각질형성세포에서 세포활성 증가, HAS2 유전자 발현 증가, 세포내 항산화 효과를 확인하였다. 올리고펩타이드를 함유한 O/W 에멀젼을 이용한 임상실험에서 피부의 탄력 개선 효과를 확인하였다. 본 연구를 통하여 발아 검은 쌀로부터 분리한 올리고펩타이드는 피부 탄력 개선을 주는 항노화 화장품 개발에 응용될 수 있을 것이라 기대된다.

• Journal of Ginseng Research
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• 제42권4호
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• pp.524-531
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• 2018

Background: Fermented black ginseng (FBG) is produced through several cycles of steam treatment of raw ginseng, at which point its color turns black. During this process, the original ginsenoside components of raw ginseng (e.g., Re, Rg1, Rb1, Rc, and Rb2) are altered, and less-polar ginsenosides are generated (e.g., Rg3, Rg5, Rk1, and Rh4). The aim of this study was to determine the effect of FBG on wound healing. Methods: The effects of FBG on tube formation and on scratch wound healing were measured using human umbilical vein endothelial cells (HUVECs) and HaCaT cells, respectively. Protein phosphorylation of mitogen-activated protein kinase was evaluated via Western blotting. Finally, the wound-healing effects of FBG were assessed using an experimental cutaneous wounds model in mice. Results and Conclusion: The results showed that FBG enhanced the tube formation in HUVECs and migration in HaCaT cells. Western blot analysis revealed that FBG stimulated the phosphorylation of p38 and extracellular signal-regulated kinase in HaCaT cells. Moreover, mice treated with $25{\mu}g/mL$ of FBG exhibited faster wound closure than the control mice did in the experimental cutaneous wounds model in mice.

• 한국전기전자재료학회논문지
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• 제15권12호
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• pp.1033-1038
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• 2002

In this study, urethane acrylate macromer was synthesized and it was used in a gel polymer electrolyte (GPE), and then its electrochemical performances were evaluated. LiCoO$_2$/GPE/graphite cells were Prepared and their performances depending on discharge currents and temperatures were evaluated. The precursor consisting of urethane acrylate (UA), hexanediol dimethacrylate (HDDA) and benzoyl peroxide (BPO) had a low viscosity relatively ionic conductivity of the gel polymer electrolyte with UA at room temperature and -20$\^{C}$ was ca. 4.5 $\times$ 10$\^$-3/S$.$cm$\^$-1/ and 1.7 x 10$\^$-3/ S$.$cm$\^$-1/, respectively GPR was stable electrochemically up to potential of 4.i V vs. Li/Li$\^$+/. LiCoO$_2$/GPE/graphite cells showed good a high-rate and a low-temperature performance.

• 한국전기전자재료학회논문지
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• 제16권11호
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• pp.994-1000
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• 2003

In this study, gel polymer electrolytes (GPE) with semi-interpenerating network of poly (methyl methacrylate) and hexanediol dimethacrylate were synthesized and their electrochemical performances were evaluated. LiCoO$_2$/GPE/graphite cells were prepared and their performances depending on discharge currents and temperatures were evaluated. The precursor containing 5 vol% curable mixture had a low viscosity relatively. GPE showed good electrochemical stability up to potential of 4.8 V vs. Li/Li$\^$+/. Ionic conductivity of the gel polymer electrolyte at room temperature and -20$^{\circ}C$ was ca. 5.9 and 1.4${\times}$10$\^$-3/ Scm$\^$-1/, respectively. LiCoO$_2$/GPE/graphite cells showed good rate capability, low-temperature performance and cycleability.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.353-357
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• 2002

We have previously demonstrated that kudzu root extracts have a hypocholesterolemic effect on rats fed diets high in fat and cholesterol. To further elucidate the mechanism involved, in this study we investigated the effect of water extracts of kudzu root, Pueraria radix, on the production of apolipoprotein B$_{100}$ (APo B$_{100}$) in HepG$_2$ liver cells and secretion of apolipoprotein B$_{48}$ (Apo B$_{48}$) in Caco$_2$ cells. Human cell lines, HepG$_2$ liver cells and Caco$_2$ intestinal epithelial cells, were grown with various concentrations (0%, 0.5%, 1.0%, 1.5%, 2.0%) of water extracts of kudzu root in the media. The kudzu root extract decreased Apo B$_{100}$ production and secretion. Treatment of HeP G$_2$ cells with the kudzu root extract also significantly decreased the intracellular total and free cholesterol concentration, and also decreased esterified cholesterol but was only significant at the highest dose of 2%. Apo B$_{48}$ production, but not secretion, from enterocytes was lowered by the kudzu root extracts. This research provided evidence that the hypocholesterolemic properties of kudzu root may be a consequence of decreased production and secretion of Apo B$_{100}$ in the liver and Apo B$_{48}$ in the intestine.

• The Korean Journal of Physiology and Pharmacology
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• 제17권5호
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• pp.427-433
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• 2013

Receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclastogenesis is accompanied by intracellular $Ca^{2+}$ mobilization in a form of oscillations, which plays essential roles by activating sequentially $Ca^{2+}$/calmodulin-dependent protein kinase, calcineurin and NFATc1, necessary in the osteoclast differentiation. However, it is not known whether $Ca^{2+}$ mobilization which is evoked in RANKL-independent way induces to differentiate into osteoclasts. In present study, we investigated $Ca^{2+}$ mobilization induced by aluminum fluoride ($AlF_4^-$), a G-protein activator, with or without RANKL and the effects of $AlF_4^-$ on the osteoclastogenesis in primary cultured mouse bone marrow-derived macrophages (BMMs). We show here that $AlF_4^-$ induces intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) oscillations, which is dependent on extracellular $Ca^{2+}$ influx. Notably, co-stimulation of $AlF_4^-$ with RANKL resulted in enhanced NFATc1 expression and formation of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells. Additionally, we confirmed that mitogen-activated protein kinase (MAPK) is also activated by $AlF_4^-$. Taken together, these results demonstrate that G-protein would be a novel modulator responsible for $[Ca^{2+}]_i$ oscillations and MAPK activation which lead to enhancement of RANKL-mediated osteoclastogenesis.

• 생명과학회지
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• 제24권2호
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• pp.112-117
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• 2014

본 연구는 복분자와 쑥 추출물이 UVB (자외선-B)에 의한 유전독성의 감소에 효과를 보이는 지를 HaCaT 세포를 이용하여 분석하였다. 자외선을 조사하지 않은 정상 세포의 세포활성은 복분자와 쑥 추출물을 처리한 경우 처리하지 않은 대조군과 차이를 나타내지 않았지만, 자외선을 조사한 세포에 복분자와 쑥 추출물을 처리한 경우 처리하지 않은 대조군에 비해 농도 의존적으로 세포 활성을 증가하였다. UVB를 조사한 후 세포의 핵 분절율은 복분자와 쑥 추출물을 처리한 경우 정상 배양액으로 배양한 대조군에 비해 핵 분절율을 각각 약 20%, 15% 정도 감소하였다. DNA 상해 회복에 반응하는 cyclobutane pyrimidine dimer (CPD)의 잔여량을 확인한 결과, 복분자와 쑥 추출만 처리한 경우 처리하지 않은 대조군에 비해 차이를 나타내지 않았으나 UVB 조사한 후 복분자와 쑥추출물을 처리한 경우 정상 배양액으로 배양한 대조군에 비해 농도의존적으로 감소하였다. 세포 상해에 반응하는 유전자인 phospho-p53과 GADD45의 단백질 수준을 Western blot으로 분석한 결과, 자외선을 조사하지 않은 정상세포에 복분자와 쑥 추출물만 처리한 경우 처리하지 않은 대조군과 비교하여 유의적인 차이를 나타내지 않았지만 UVB 조사한 후 복분자와 쑥 추출물을 처리한 경우 농도 의존적으로 phospho-p53과 GADD45 단백질 발현량이 감소하였다. 위의 결과에서 복분자와 쑥 추출물은 UVB에 유도된 DNA 상해와 p53 및 GADD45와 같은 상해 반응단백질의 수준을 감소시키는 것으로 보아 UVB에 의해 손상된 세포의 유전독성을 회복하는 효능이 있다고 생각된다.

• Journal of Ginseng Research
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• 제43권2호
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• pp.326-334
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• 2019

Background: The objective of our study was to analyze the neuroprotective effects of ginsenoside derivatives Rb1, Rb2, Rc, Rd, Rg1, and Rg3 against glutamate-mediated neurotoxicity in HT22 hippocampal mouse neuron cells. Methods: The neuroprotective effect of ginsenosides were evaluated by measuring cell viability. Protein expressions of mitogen-activated protein kinase (MAPK), Bcl2, Bax, and apoptosis-inducing factor (AIF) were determined by Western blot analysis. The occurrence of apoptotic and death cells was determined by flow cytometry. Cellular level of $Ca^{2+}$ and reactive oxygen species (ROS) levels were evaluated by image analysis using the fluorescent probes Fluor-3 and 2',7'-dichlorodihydrofluorescein diacetate, respectively. In vivo efficacy of neuroprotection was evaluated using the Mongolian gerbil of ischemic brain injury model. Result: Reduction of cell viability by glutamate (5 mM) was significantly suppressed by treatment with ginsenoside Rb2. Phosphorylation of MAPKs, Bax, and nuclear AIF was gradually increased by treatment with 5 mM of glutamate and decreased by co-treatment with Rb2. The occurrence of apoptotic cells was decreased by treatment with Rb2 ($25.7{\mu}M$). Cellular $Ca^{2+}$ and ROS levels were decreased in the presence of Rb2, and in vivo data indicated that Rb2 treatment (10 mg/kg) significantly diminished the number of degenerated neurons. Conclusion: Our results suggest that Rb2 possesses neuroprotective properties that suppress glutamate-induced neurotoxicity. The molecular mechanism of Rb2 is by suppressing the MAPKs activity and AIF translocation.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
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• pp.79-85
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• 1990

The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and comitogenic activities in human peripheral blood Iymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose. DEAE-Sepharose) yielded a semi-purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in Iymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.