• Journal of Plant Biotechnology
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• v.31 no.4
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• pp.279-283
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• 2004

To lily (Lilium longflorum cv. Georgia) pollen, impacts by some physical, chemical and biological factors were examined in respects of its growth and transient gene expression via agro-infiltration. Rolling movement in liquid medium or vacuum pressure during Agro-infiltration was regarded as a impact that should be minimized for normal pollen growth. Pollen growth was maintained well in relatively broad range of temperature (19 to 27$^{\circ}C$) or pH (5.0 to 8.0). Chemical factors such as cefotaxime (up to 300mg/L), acetosyringone (up to 800 $\mu$M) and syringealdehyde (up to 800 $\mu$M) did not show any harmful effects but kanamycin severely did even at concentration as low as 25mg/L in some cases. For GUS gene expression, acetosyringone at 200 to 400 $\mu$M slightly improved the efficiency while syringealdehyde did not. Brief agro-infiltration followed by 18 hr of co-incubation of pollen along with Agrobacterium was suggested as a condition basically required for the transient expression system using lily pollen regardless of the presence of acetosyringone.

• Journal of Acupuncture Research
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• v.21 no.5
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• pp.205-218
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• 2004

Objectives : The purpose of this experiment is to study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Carthami Flos infusion solution(CTT-HAS). Methods : We injected Carthami Flos infusion solution into Chung-wan(CV12) of C57BL/6 mouse which is corresponding to human Chung-wan(CV12). We observed its effect on the number of $CD25^{+}/CD4^{+}$, $CD8^{+}/CD3e^{+}$, $CD69^{+}/B220^{+}$, $NK^{+}/CD3e^{+}$ cells in mouse PBMCs, the number of the pulmonary colony, and the effect on MST and ILS of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results Conclusions : 1. The spleen cells proliferation of the sample groups treated with CTT-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^{+}/CD4^{+}$, $CD8^{+}/CD3e^{+}$, $CD69^{+}/B220^{+}$, $NK^{+}/CD3e^{+}$ cells in C57BL/6 mouse PBMCs of the sample groups treated with CTT herbal-acupuncture has increased compared with that of the control group. 3. The lung colony number of the sample groups CTT Herbal-acupuncture has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups CTT herbal-acupuncture have increased significantly compared with those of the control group.

• Journal of Acupuncture Research
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• v.21 no.6
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• pp.85-102
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• 2004

Objective : The purpose of this experiment is to study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Sinomenii acuti lignun infusion solution(SAL-HAS). Methods : We injected Sinomenii acuti Lignum infusion solution into Chung-wan(CV12) of C57BL/6 mouse which is corresponding to human Chung-wan(CV12). We observed its effect on the nunter of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK^+/CD3e^+$ cells in mouse PBMCs, the number of the pulmonary colony, and the effect on MST and ILs of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results & Conclusions : 1. The spleen cells proliferation of the sample groups treated with SAL-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK^+/CD3e^+$ cells in C57BL/6 mouse PBMCs of the sample groups treated with SAL herbal-acupuncture has increased compared with that of the control group. 3. The lung colony number of the sample groups SAL Herbal-acupuncture has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups SAL herbal-acupuncture have increased significantly compared with those of the control group.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.284-287
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• 2010

A new standard rose cv. 'Honey Lemon' was bred by the cross between white standard cv. 'Escimo' and light pink standard cv. 'Medeo' at the National Institute of Horticultural Research Institute. The cross was made in 1999 and 'Honey Lemon' was finally selected in 2003 after investigating characteristics for three years from 2001 to 2003. 'Honey Lemon', a white standard cultivar grows vigorously and has good flower shape. The major characteristics of this cultivar are $248.0stems/m^2/year$ in yield, 53.0 cm in length of cut flower, 8.0 cm in flower diameter, 17.0 in petal number, and 16.9 days in vase life. This cultivar can be propagated by both cutting and grafting. The consumer's preference of this cultivar is relatively higher than that of control cv., 'Tineke'. (Registration number: 1151).

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.235-243
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• 2014

This research was conducted to investigate the influence of leaching fractions (LF) in each irrigation or fertigation on plant growth and changes in chemical properties of root media during the production of seedling grafts of tomato. Two root media containing Sphagnum peat moss plus vermiculite (5:5, v/v, PV) and coir dust plus vermiculite (5:5, v/v, CV) were formulated and pre-planting fertilizers were incorporated during formulation. Then, each medium was packed into 50 cell (volume 33 cc) and 105 cell (volume 18 cc) trays and the rootstock (cv. J3B Strong) and scion (cv. Sunmyung) were grown, respectively. The seedlings were grafted at 31 days after sowing and then the cut seedling grafts (Sunmyung scion/J3B Strong rootstock) were planted into 50 cell plug trays containing each of the two root media. After induction of the graft union and new adventitious roots for 7 days, the seedling grafts were fed with fertilizer solution once a week containing 4 different N concentrations (0, 50, 100, $200mg{\cdot}L^{-1}$). When determined after 31 days from seed sowing, the highest fresh weights of the root stock seedlings were obtained with 0.75 LF in PV (8.96g/seedling) and CV (7.11g/seedling) mixes. The EC of the both mixes were 0.93 and $1.09dS{\cdot}m^{-1}$, respectively. The fresh weights of the scion seedlings 31 days after seed sowing were 4.29g with 0.50 LF in the PV and 3.13g with 0.50 LF in the CV. The root medium ECs of the two treatments were 0.76 and $1.34dS{\cdot}m^{-1}$, respectively. Fresh weights of the seedling grafts grown for 31 days were greatly influenced by post-planting fertilizer concentrations. The heavier plants were obtained in $100mg{\cdot}L^{-1}$ N treatment than any other treatments in same mixes. The substrate ECs in these two treatments were 0.98 and $1.93dS{\cdot}m^{-1}$, respectively, indicating that the desirable range of soluble salts in soil extracts is higher in the CV mix than the PV mix. Results of this study suggest that optimum EC range is different in each medium and LF need to be adjusted differently for each root medium to produce high quality seedling grafts of tomato.

• Korean Journal of Organic Agriculture
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• v.14 no.2
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• pp.191-204
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• 2006

This study was conducted to identify the effect of planting date and cultivation method on soybean growth and yield in paddy field. The plant height of soybeans in single cropping(SC) was higher than those in cultivating after barley culture (CB). Considering planting date and cultivation method, plant height tend to be higher in SC with level row cultivation(LR) and in CB with high ridge cultivation(HR). In this SC method, nodule formation in LR was better than in HR, but number of nodules of cv. Hwangkeumkong was highest, regardless of planting date and cultivation method. In the progress of growth stage, leaf areas of all cultivars were distinctively increased in CB than in SC. However, dry weight of top plants and roots in SC was comparably higher than that in CB due to growing periods of the soybeans. In cultivation methods, general type of dry weight of top plants was higher in LR than in HR, however, root dry weight was via verses. Growth responses varied depending on cultivars, cultivation methods, and planting date and these factors affected to shoot root (T/R) ratio. The T/R ratios in LR and SC were higher than those in HR and CB. In R8 stage, number of pods and ripened seed varied depending on cultivars. cv. Hwaeomputkong, which showed early maturing trait, was lowest. However, both yield factors tended to be higher in HR and CB than in LR and SC. The ratios of ripened seeds percentage of cv. Hwangkeumkong and cv. Eunhakong were higher in CB than in SC. However, yields of cv. Daewonkon and cv. Taekwangkong were higher in CB than in SC.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.94-101
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• 2004

Commercial value of soybean sprouts should be affected by their morphology including the lateral roots. This study was carried out to determine the effect of benzyladenopurine (BA) concentration on growth and morphology of soybean sprouts in order to compare them with the selling products collected from the markets. Four cultivars (cv. Eunhakong, Pungsannamulkong, Sowonkong, and Junjery) were cultured for 6 days after imbibed for 5 hours into different BA solutions (0, 1,2,4, and 8 ppm). On the 6th day, hypocotyl length was measured to calculate the composition rate of ＞ 7 cm (A), 4 to 7 cm (B), ＜ 4 cm (C) and non-germination (D), and the ratio of hypocotyl to root length (H/R ratio) on the base of hypocotyl length as well as lateral root, hypocotyl diameter, and fraction fresh and dry weights. The composition rates classified with hypocotyl length showed big differences between 4 cultivars in class A and C; Class A, in which cv. Sowonkong and Junjery had higher rates than cv. Eunhakong and Pungsannamulkong, was decreased with BA concentration of higher than 4 ppm, but class C showed the reverse result to class A. Any lateral root was not formed in higher than 4 ppm BA solutions although fewer in cv. Pungsannamulkong and Junjery than in the two other cultivars. Hypocotyl and root lengths were decreased with increased BA concentrations, and in all BA concentration, hypocotyl length of cv. Junjery was the longest. Hypocotyl diameter, hypocotyl and total fresh weights were thicker and heavier in 2 to 4 ppm BA concentrations than in the other ones, although in case of BA application, cv, Eunhakong and Sowonkong were heavier. The H/R ratios were increased with increased BA concentration. The ratios of nearly all the selling products ranged from 1 to 2 ppm when compared to them from our experiment.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.16 no.4
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• pp.165-170
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• 2016

The XLPE(or CV) cables installed to deliver high electric power from generator at the power station have operating for the last 40 years in Korea. In 6-8 years from the time of initial operation, the cables in operation start a deteriorating process, depending on the installation and usage conditions. Some cables, even before 6-8 years from the installation, can cause accidents due to the faulty construction or other inadequate environments. In order to prevent cable accident in advance, the regular auditing of power cables are required. For a systematic realization of surveillance and prevention of accidents, we have invented a measuring device. In this paper, we present the device installed at Korean Western Power Co. Ltd., in order to detect the deterioration status of insulation resistances of dielectrics in the cables. We present that the measured results by the device we developed show the deteriorating processes in the cables in operation.

• Korean Journal of Acupuncture
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• v.27 no.2
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• pp.57-70
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• 2010

Objectives : To investigate the meaning and the importance of needling or moxibustion- prohibited acupoints in Chimgugapeulgyeong(鍼灸甲乙經). Methods : We found needling or moxibustion-prohibited acupoints in Chimgugapeulgyeong, then investigated the causes of the prohibitions from the various literatures and the anatomical structures near the acupoints. Results : In Chimgugapeulgyeong, the needling and moxibustion-prohibited points were ST9, ST17, ST32, CV5, and CV15. The needling-prohibited points were LU2, LI13, ST12, BL56, KI2, KI7, TE8, TE19, GB3, CV8, GV24, and jwagak(左角). The moxibustion-prohibited points were LU3, LU8, ST1, ST7, ST8, ST30, ST33, BL5, BL6, BL15, BL30, TE18, TE21, TE23, GB22, GB33, GB42, GV6, GV15, GV16, GV17 and GV25. The major cause of developing prohibited needling or moxibustion was due to the possibility to damage vessels or organs near them; other causes were side effects brought by applying wrong stimulating method or inducing women sterilized. Conclusions : The prohibition of needling or moxibustion on the points found in Chimgugapeulgyeong had acceptable causes. Therefore the techniques of needling or moxibustion on the acupoint should be performed with care.

• Journal of Acupuncture Research
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• v.29 no.6
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• pp.35-45
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• 2012

Objectives : This study was designed to investigate Effects of Sujeom powder(SJP) pharmacopuncture Injected at Jung-wan($CV_{12}$) in rats with caerulein-induced acute pancreatitis(AP). Methods : We examined changes of organ weight, histology, immunohistochemistry and gene expression of cycolooxygenase 2(COX-2) in the pancreas. Twenty adult male Sprague-Dawley rats were divided into four groups as follow: normal(Nor), caerulein-induced(Con), caerulein+SJP pharmacopuncture 0.2mL injected at Jung-wan($CV_{12}$)(SA), and caerulein+SJP pharmacopuncture 0.8 mL injected at Jung-wan($CV_{12}$)(SB) groups. Pancreatic tissues of rats from all groups were removed for histological observation and light microscopic examination. Interleukin-6(IL-6) levels were determined spectrophotometrically. Results : The ratio of pancreas/body weights was significantly(p<0.05) increased in the Con, the SA and the SB compared with the Nor, but was slightly decreased in the SA and in the SB groups compared with the Con. Caerulein administration has significantly(p<0.05) increased in the levels of amylase, but the SA, the SB significantly(p<0.05) decreased in the levels of these enzyme. The levels of amylase were increased significantly with caerulein administration, but were inhibited significantly in the SA and in the SB groups. Interleukin-6(IL-6) levels were significantly(p<005) increased in all groups compared with the Nor, especially in the SB. were significantly increased. The levels of Tumor necrosis factor(TNF)-${\alpha}$ levels were significantly increased in all groups compared with the Nor. In the conclusion, the datum of IL-6 and TNF-${\alpha}$ are suggested that the inflamation was still existed actively at a point of measurement(24 hours later). The COX-2 positive materials are observed in the pancreas from the Con, but these positive materials are decreased in the SJP pharmacopuncture at Jung-wan($CV_{12}$) treatment group. Conclusion : SJP pharmacopuncture injected at Jung-wan($CV_{12}$) is potentially capable of limiting pancreatic damage during AP by restoring the fine structure of acinar cells and tissues. Therefore we can say that SJP pharmacopuncture Injected at Jung-wan($CV_{12}$) may have beneficial effects in the treatment of caerulein-induded AP. Further studies about the adequate amount of the SJP pharmacopuncture and about more effective route of administration is still required.