• Proceedings of KOSOMES biannual meeting
• /
• 2017.04a
• /
• pp.215-215
• /
• 2017

• Journal of the Korea Convergence Society
• /
• v.8 no.10
• /
• pp.323-332
• /
• 2017

This study is convergence study of path model for the relationship among self esteem(SE), critical thinking disposition(CTD), problem solving skills(PSS) and motivation toward learning(MTL). The survey was performed on 207 nursing students in one college. Data was collected using a structured questionnaires and analysed with PASW 18.0 and AMOS 18.0. Model fit indices were in good agreement with recommended level(${\chi}^2/df=2.128$, GFI=.995, AGFI=.949, RMSEA=.074, CFI=.993, NFI=.988). Based on the path model, SE(${\beta}=.205$), PSS(${\beta}=.420$) showed a direct effect on MTL while CTD(${\beta}=.182$) showed a indirect effect on MTL. The SE showed a direct effect on CTD(${\beta}=.398$) and CTD(${\beta}=.433$) showed a direct effect on PSS. The findings indicate the necessity of developing educational programs to enhance nursing student's SE for increasing of MTL.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.6
• /
• pp.789-793
• /
• 2012

Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryonic genome activation (EGA). RNA polymerase II (RNA Pol II) is responsible for the synthesis of mRNAs and most small nuclear RNAs, and consists of 12 subunits, the largest of which characteristically harbors a unique C-terminal domain (CTD). Transcriptional activity of RNA Pol II is highly regulated, in particular, by phosphorylation of serine residues in the CTD. Here, we have shown the presence of RNA Pol II CTD phosphoisoforms in porcine oocytes and preimplantation embryos. The distribution pattern as well as phosphorylation dynamics in germinal vesicles and during embryogenesis differed in developmental stages with these isoforms, indicating a role of RNA Pol II CTD phosphorylation at the serine residue in transcriptional activation during both oocyte growth and embryonic genome activation. We additionally examined the effects of the RNA Pol II inhibitor, ${\alpha}$-amanitin, on embryo development. Our results show that inhibition of polymerase, even at very early stages and for a short period of time, dramatically impaired blastocyst formation. These findings collectively suggest that the functionality of maternal RNA Pol II, and consequently, expression of early genes regulated by this enzyme are essential for proper embryo development.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• v.12 no.1
• /
• pp.115-124
• /
• 2001

Objective：The purpose of this study was to compare the clinical features of children with three subdiagnoses of tic disorder(transient tic disorder(TTD), chronic tic disorder(CTD) and Tourette’s disorder (TD)) and to exam the factors related with illness severity of them. Method：Subjects were 69 children who met DSM-IV criteria for tic disorder and 43 control children. All subjects were investigated demographic and clinical factors related to tic. The severity of tic was assessed with the Yale Global Tic Severity Scale(YGTSS) in tic disorder children. The Child Behavior Checklist(CBCL) was accomplished by parents of all subjects. Results：Children with TTD had not only shorter duration of tic symtoms but also milder tic severity and impairment than those with CTD and TD. They also had significantly lower scores on most CBCL subscales than children with CTD and TD, while they were similar with controls in all the CBCL subcale scores except aggressive behavior. Children with CTD and those with TD were similar to each other in clinical variables except number of tic symptom and scores on CBCL social problem subscale. The interference and intensity of motor tic symptoms and duration of tic symptoms were significant predictors of global impairment score on YGTSS, while the presence of comorbid ADHD was a preictor of the total behavior problem score of CBCL. Conclusion：These findings indicate that duration of tic symptoms and the presence of comorbid ADHD as well as the severity of tic symptoms strongly associated with the illness severity of children with tic disorder. These results also suggest that those clinical factors may be more important for assessing the severity of illness and determining the treatment strategy than the sub-diagnosis itself in children with tic disorder.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.13 no.3
• /
• pp.178-185
• /
• 2005

Crack tip displacement is originated by tensile stress component, s and shear stress component, t on pure Mode I and pure Mode II. The crack tip displacement(CTD) depends on combined types of different two stress components under mixed-mode loading conditions (MMLC). Thus, the analysis of crack tip displacement must be CTD vector, dv which is composition of ds and dt under MMLC. In this paper, various effects of MMLC on the crack closure are studied experimentally. The crack closure magnitude is calculated from the information of crack tip displacement under MMLC. This information has been obtained from the high resolution optical microscope in direct observations of crack displacement behavior at the crack tip. Observed crack tip displacement is analyzed by using CTD vector to determine crack opening load. The various effects of MMLC on the crack closure are explained using crack opening ratio with crack length and mode mixture. The effective stress intensity factor considering crack closure is also discussed.

• BMB Reports
• /
• v.47 no.10
• /
• pp.593-598
• /
• 2014

RNA polymerase II carboxyl-terminal domain (RNAPII CTD) phosphatases are responsible for the dephosphorylation of the C-terminal domain of the small subunit of RNAPII in eukaryotes. Recently, we demonstrated the identification of several interacting partners with human small CTD phosphatase1 (hSCP1) and the substrate specificity to delineate an appearance of the dephosphorylation catalyzed by SCP1. In this study, using the established cells for inducibly expressing hSCP1 proteins, we monitored the modification of ${\beta}$-O-linked N-acetylglucosamine (O-GlcNAc). O-GlcNAcylation is one of the most common post-translational modifications (PTMs). To gain insight into the PTM of hSCP1, we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutinin-precipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the $Ser^{41}$ residue of hSCP1 as the O-GlcNAc modification site. These results suggest that hSCP1 may be an O-GlcNAcylated protein in vivo, and its N-terminus may function a possible role in the PTM, providing a scaffold for binding the protein(s).

• The Journal of the Acoustical Society of Korea
• /
• v.28 no.3
• /
• pp.187-197
• /
• 2009

The bubbles are created by waves, raindrops, water collision, vessels sailing at sea, life activities of various marine organisms in the ocean and other sources. The bubbles affect the intensity and sound speed of acoustic waves in the ocean. We indirectly observed bubbles in order to understand the creation of and the effects of bubbles on sound waves, using an Acoustic Bubble Spectrometer (ABS) and CTD, from 04:00 to 17:00, 19 September, 2007. We also analyzed the correlation of wind speed and the generation of bubbles, the amount of bubbles, and the sound speed variation at 50, 60, and 70 kHz. Finally, We simulated the way how bubbles affect sound transmission based on the analysis results.

• Korean Journal of Microbiology
• /
• v.55 no.2
• /
• pp.123-130
• /
• 2019

Chelatase catalyzes the insertion of divalent metal into tetrapyrrole and plays a key role in the biosynthesis of metallated tetrapyrroles, such as cobalamin, siroheme, heme, and chlorophyll. SirB is a sirohydrochlorin (SHC) chelatase that generates cobalt-SHC or iron-SHC by inserting cobalt or iron into the center of sirohydrochlorin tetrapyrrole. To provide structural insights into the metal-binding and SHC-recognition mechanisms of SirB, we determined the crystal structure of SirB from Bacillus subtilis subsp. spizizenii (bssSirB) in complex with cobalt ions. bssSirB forms a monomeric ${\alpha}/{\beta}$ structure that consists of two domains, an N-terminal domain (NTD) and a C-terminal domain (CTD). The NTD and CTD of bssSirB adopt similar structures with a four-stranded ${\beta}-sheet$ that is decorated by ${\alpha}-helices$. bssSirB presents a highly conserved cavity that is generated between the NTD and CTD and interacts with a cobalt ion on top of the cavity using two histidine residues of the NTD. Moreover, our comparative structural analysis suggests that bssSirB would accommodate an SHC molecule into the interdomain cavity. Based on these structural findings, we propose that the cavity of bssSirB functions as the active site where cobalt insertion into SHC occurs.

• BMB Reports
• /
• v.49 no.6
• /
• pp.319-324
• /
• 2016

RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg⁺²-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2.

• Communications of the Korean Mathematical Society
• /
• v.27 no.4
• /
• pp.843-849
• /
• 2012

Let G = (V, E) be a graph with chromatic number ${\chi}(G)$. dominating set D of G is called a chromatic transversal dominating set (ctd-set) if D intersects every color class of every ${\chi}$-partition of G. The minimum cardinality of a ctd-set of G is called the chromatic transversal domination number of G and is denoted by ${\gamma}_{ct}$(G). In this paper we characterize the class of trees, unicyclic graphs and cubic graphs for which the chromatic transversal domination number is equal to the connected domination number.